OA11998A - Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins. - Google Patents

Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins. Download PDF

Info

Publication number: OA11998A
Authority: OA; OAPI
Prior art keywords: leu; ala; val; gly; avec
Prior art date: 1999-08-12

Application number

OA1200200025A

Other languages

English (en)

Inventor

Yan Chen

Claes Gustafsson

Anke Krebber

Jeremy Stephen Minshull

Sun Ai Raillard

Kim Jonelle Stutzman-Engwall

Original Assignee

Pfizer Prod Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-08-12

Filing date

2000-07-24

Publication date

2006-04-18

2000-07-24 Application filed by Pfizer Prod Inc filed Critical Pfizer Prod Inc

2006-04-18 Publication of OA11998A publication Critical patent/OA11998A/en

Links

241001468227 Streptomyces avermitilis Species 0.000 title claims abstract description 243
108090000623 proteins and genes Proteins 0.000 title claims abstract description 243
239000005660 Abamectin Substances 0.000 title claims abstract description 222
230000008859 change Effects 0.000 claims abstract description 106
108091033319 polynucleotide Proteins 0.000 claims abstract description 95
102000040430 polynucleotide Human genes 0.000 claims abstract description 95
239000002157 polynucleotide Substances 0.000 claims abstract description 95
239000013598 vector Substances 0.000 claims abstract description 55
108700028369 Alleles Proteins 0.000 claims description 157
230000035772 mutation Effects 0.000 claims description 112
125000000539 amino acid group Chemical group 0.000 claims description 98
238000000034 method Methods 0.000 claims description 79
238000006467 substitution reaction Methods 0.000 claims description 75
150000001413 amino acids Chemical class 0.000 claims description 67
125000003275 alpha amino acid group Chemical group 0.000 claims description 65
238000004519 manufacturing process Methods 0.000 claims description 52
239000000203 mixture Substances 0.000 claims description 35
125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 32
241000187391 Streptomyces hygroscopicus Species 0.000 claims description 27
125000000113 cyclohexyl group Chemical group [H]C1([H])C([H])([H])C([H])([H])C([H])(*)C([H])([H])C1([H])[H] 0.000 claims description 21
241000187747 Streptomyces Species 0.000 claims description 20
230000002829 reductive effect Effects 0.000 claims description 17
239000002253 acid Substances 0.000 claims description 16
239000001963 growth medium Substances 0.000 claims description 9
229910052698 phosphorus Inorganic materials 0.000 claims description 8
229910052717 sulfur Inorganic materials 0.000 claims description 8
238000012258 culturing Methods 0.000 claims description 7
102200012537 rs111033646 Human genes 0.000 claims description 7
102200049565 rs121913045 Human genes 0.000 claims description 7
241000970979 Streptomyces griseochromogenes Species 0.000 claims description 6
230000008569 process Effects 0.000 claims description 6
102220044659 rs587781460 Human genes 0.000 claims description 6
229910052739 hydrogen Inorganic materials 0.000 claims description 5
239000004281 calcium formate Substances 0.000 claims description 4
241000282326 Felis catus Species 0.000 claims description 3
SGPGESCZOCHFCL-UHFFFAOYSA-N Tilisolol hydrochloride Chemical compound [Cl-].C1=CC=C2C(=O)N(C)C=C(OCC(O)C[NH2+]C(C)(C)C)C2=C1 SGPGESCZOCHFCL-UHFFFAOYSA-N 0.000 claims 51
VPZXBVLAVMBEQI-UHFFFAOYSA-N glycyl-DL-alpha-alanine Natural products OC(=O)C(C)NC(=O)CN VPZXBVLAVMBEQI-UHFFFAOYSA-N 0.000 claims 11
108010050848 glycylleucine Proteins 0.000 claims 10
241000880493 Leptailurus serval Species 0.000 claims 7
108010079364 N-glycylalanine Proteins 0.000 claims 7
102200005898 rs137852378 Human genes 0.000 claims 7
AJHCSUXXECOXOY-UHFFFAOYSA-N N-glycyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)CN)C(O)=O)=CNC2=C1 AJHCSUXXECOXOY-UHFFFAOYSA-N 0.000 claims 5
FKYWFUYPVKLJLP-DCAQKATOSA-N Ser-Pro-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CO FKYWFUYPVKLJLP-DCAQKATOSA-N 0.000 claims 5
AEFJNECXZCODJM-UWVGGRQHSA-N Val-Val-Gly Chemical compound CC(C)[C@H]([NH3+])C(=O)N[C@@H](C(C)C)C(=O)NCC([O-])=O AEFJNECXZCODJM-UWVGGRQHSA-N 0.000 claims 5
108010092114 histidylphenylalanine Proteins 0.000 claims 5
108010090894 prolylleucine Proteins 0.000 claims 5
XEPSCVXTCUUHDT-AVGNSLFASA-N Arg-Arg-Leu Natural products CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](N)CCCN=C(N)N XEPSCVXTCUUHDT-AVGNSLFASA-N 0.000 claims 4
HYIFFZAQXPUEAU-QWRGUYRKSA-N Leu-Gly-Leu Chemical compound CC(C)C[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CC(C)C HYIFFZAQXPUEAU-QWRGUYRKSA-N 0.000 claims 4
IRAUYEAFPFPVND-UVBJJODRSA-N Val-Trp-Ala Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](N)C(C)C)C(=O)N[C@@H](C)C(O)=O)=CNC2=C1 IRAUYEAFPFPVND-UVBJJODRSA-N 0.000 claims 4
108010027668 glycyl-alanyl-valine Proteins 0.000 claims 4
108010057821 leucylproline Proteins 0.000 claims 4
108010073025 phenylalanylphenylalanine Proteins 0.000 claims 4
MNZHHDPWDWQJCQ-YUMQZZPRSA-N Ala-Leu-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)NCC(O)=O MNZHHDPWDWQJCQ-YUMQZZPRSA-N 0.000 claims 3
OYJCVIGKMXUVKB-GARJFASQSA-N Ala-Leu-Pro Chemical compound C[C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@@H]1C(=O)O)N OYJCVIGKMXUVKB-GARJFASQSA-N 0.000 claims 3
AETQNIIFKCMVHP-UVBJJODRSA-N Ala-Trp-Arg Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O AETQNIIFKCMVHP-UVBJJODRSA-N 0.000 claims 3
FKJQNJCQTKUBCD-XPUUQOCRSA-N Gly-Ala-His Chemical compound NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)O FKJQNJCQTKUBCD-XPUUQOCRSA-N 0.000 claims 3
KFKWRHQBZQICHA-STQMWFEESA-N L-leucyl-L-phenylalanine Natural products CC(C)C[C@H](N)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 KFKWRHQBZQICHA-STQMWFEESA-N 0.000 claims 3
BQSLGJHIAGOZCD-CIUDSAMLSA-N Leu-Ala-Ser Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O BQSLGJHIAGOZCD-CIUDSAMLSA-N 0.000 claims 3
KZNQNBZMBZJQJO-UHFFFAOYSA-N N-glycyl-L-proline Natural products NCC(=O)N1CCCC1C(O)=O KZNQNBZMBZJQJO-UHFFFAOYSA-N 0.000 claims 3
BRPKEERLGYNCNC-NHCYSSNCSA-N Val-Glu-Arg Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@H](C(O)=O)CCCN=C(N)N BRPKEERLGYNCNC-NHCYSSNCSA-N 0.000 claims 3
108010044056 leucyl-phenylalanine Proteins 0.000 claims 3
PKOHVHWNGUHYRE-ZFWWWQNUSA-N (2s)-1-[2-[[(2s)-2-amino-3-(1h-indol-3-yl)propanoyl]amino]acetyl]pyrrolidine-2-carboxylic acid Chemical compound O=C([C@H](CC=1C2=CC=CC=C2NC=1)N)NCC(=O)N1CCC[C@H]1C(O)=O PKOHVHWNGUHYRE-ZFWWWQNUSA-N 0.000 claims 2
ZFDBLGBZACCLMH-UHFFFAOYSA-N 2-[[2-[2-[2-[(2-aminoacetyl)amino]propanoylamino]propanoylamino]-4-methylpentanoyl]amino]-4-methylpentanoic acid Chemical compound CC(C)CC(C(O)=O)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(C)NC(=O)CN ZFDBLGBZACCLMH-UHFFFAOYSA-N 0.000 claims 2
KVWLTGNCJYDJET-LSJOCFKGSA-N Ala-Arg-His Chemical compound C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N KVWLTGNCJYDJET-LSJOCFKGSA-N 0.000 claims 2
KIUYPHAMDKDICO-WHFBIAKZSA-N Ala-Asp-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)NCC(O)=O KIUYPHAMDKDICO-WHFBIAKZSA-N 0.000 claims 2
SMCGQGDVTPFXKB-XPUUQOCRSA-N Ala-Gly-Val Chemical compound CC(C)[C@@H](C(O)=O)NC(=O)CNC(=O)[C@H](C)N SMCGQGDVTPFXKB-XPUUQOCRSA-N 0.000 claims 2
IPZQNYYAYVRKKK-FXQIFTODSA-N Ala-Pro-Ala Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(O)=O IPZQNYYAYVRKKK-FXQIFTODSA-N 0.000 claims 2
HOVPGJUNRLMIOZ-CIUDSAMLSA-N Ala-Ser-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)[C@H](C)N HOVPGJUNRLMIOZ-CIUDSAMLSA-N 0.000 claims 2
ARHJJAAWNWOACN-FXQIFTODSA-N Ala-Ser-Val Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(O)=O ARHJJAAWNWOACN-FXQIFTODSA-N 0.000 claims 2
IOFVWPYSRSCWHI-JXUBOQSCSA-N Ala-Thr-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](C)N IOFVWPYSRSCWHI-JXUBOQSCSA-N 0.000 claims 2
BOKLLPVAQDSLHC-FXQIFTODSA-N Ala-Val-Cys Chemical compound C[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CS)C(=O)O)N BOKLLPVAQDSLHC-FXQIFTODSA-N 0.000 claims 2
VHAQSYHSDKERBS-XPUUQOCRSA-N Ala-Val-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)NCC(O)=O VHAQSYHSDKERBS-XPUUQOCRSA-N 0.000 claims 2
KJGNDQCYBNBXDA-GUBZILKMSA-N Arg-Arg-Cys Chemical compound C(C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CS)C(=O)O)N)CN=C(N)N KJGNDQCYBNBXDA-GUBZILKMSA-N 0.000 claims 2
OVVUNXXROOFSIM-SDDRHHMPSA-N Arg-Arg-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CCCN=C(N)N)N)C(=O)O OVVUNXXROOFSIM-SDDRHHMPSA-N 0.000 claims 2
HJAICMSAKODKRF-GUBZILKMSA-N Arg-Cys-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O HJAICMSAKODKRF-GUBZILKMSA-N 0.000 claims 2
CYXCAHZVPFREJD-LURJTMIESA-N Arg-Gly-Gly Chemical compound NC(=N)NCCC[C@H](N)C(=O)NCC(=O)NCC(O)=O CYXCAHZVPFREJD-LURJTMIESA-N 0.000 claims 2
UGZUVYDKAYNCII-ULQDDVLXSA-N Arg-Phe-Leu Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(C)C)C(O)=O UGZUVYDKAYNCII-ULQDDVLXSA-N 0.000 claims 2
BSYKSCBTTQKOJG-GUBZILKMSA-N Arg-Pro-Ala Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(O)=O BSYKSCBTTQKOJG-GUBZILKMSA-N 0.000 claims 2
PYDIIVKGTBRIEL-SZMVWBNQSA-N Arg-Trp-Pro Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N1CCC[C@H]1C(O)=O PYDIIVKGTBRIEL-SZMVWBNQSA-N 0.000 claims 2
GMRGSBAMMMVDGG-GUBZILKMSA-N Asn-Arg-Arg Chemical compound C(C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)NC(=O)[C@H](CC(=O)N)N)CN=C(N)N GMRGSBAMMMVDGG-GUBZILKMSA-N 0.000 claims 2
CPYHLXSGDBDULY-IHPCNDPISA-N Asn-Trp-Phe Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O CPYHLXSGDBDULY-IHPCNDPISA-N 0.000 claims 2
KGHLGJAXYSVNJP-WHFBIAKZSA-N Asp-Ser-Gly Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CO)C(=O)NCC(O)=O KGHLGJAXYSVNJP-WHFBIAKZSA-N 0.000 claims 2
OQXDUSZKISQQSS-GUBZILKMSA-N Glu-Lys-Ala Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O OQXDUSZKISQQSS-GUBZILKMSA-N 0.000 claims 2
WGYHAAXZWPEBDQ-IFFSRLJSSA-N Glu-Val-Thr Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O WGYHAAXZWPEBDQ-IFFSRLJSSA-N 0.000 claims 2
UGVQELHRNUDMAA-BYPYZUCNSA-N Gly-Ala-Gly Chemical compound [NH3+]CC(=O)N[C@@H](C)C(=O)NCC([O-])=O UGVQELHRNUDMAA-BYPYZUCNSA-N 0.000 claims 2
LCNXZQROPKFGQK-WHFBIAKZSA-N Gly-Asp-Ser Chemical compound NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(O)=O LCNXZQROPKFGQK-WHFBIAKZSA-N 0.000 claims 2
PAWIVEIWWYGBAM-YUMQZZPRSA-N Gly-Leu-Ala Chemical compound NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(O)=O PAWIVEIWWYGBAM-YUMQZZPRSA-N 0.000 claims 2
JNGHLWWFPGIJER-STQMWFEESA-N Gly-Pro-Tyr Chemical compound NCC(=O)N1CCC[C@H]1C(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 JNGHLWWFPGIJER-STQMWFEESA-N 0.000 claims 2
YDIDLLVFCYSXNY-RCOVLWMOSA-N Gly-Val-Asn Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)O)NC(=O)CN YDIDLLVFCYSXNY-RCOVLWMOSA-N 0.000 claims 2
VPZXBVLAVMBEQI-VKHMYHEASA-N Glycyl-alanine Chemical compound OC(=O)[C@H](C)NC(=O)CN VPZXBVLAVMBEQI-VKHMYHEASA-N 0.000 claims 2
VAXBXNPRXPHGHG-BJDJZHNGSA-N Ile-Ala-Leu Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)O)N VAXBXNPRXPHGHG-BJDJZHNGSA-N 0.000 claims 2
CNMOKANDJMLAIF-CIQUZCHMSA-N Ile-Thr-Ala Chemical compound CC[C@H](C)[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(O)=O CNMOKANDJMLAIF-CIQUZCHMSA-N 0.000 claims 2
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DRWMRVFCKKXHCH-BZSNNMDCSA-N Leu-Phe-Leu Chemical compound CC(C)C[C@H]([NH3+])C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C([O-])=O)CC1=CC=CC=C1 DRWMRVFCKKXHCH-BZSNNMDCSA-N 0.000 claims 2
BMVFXOQHDQZAQU-DCAQKATOSA-N Leu-Pro-Asp Chemical compound CC(C)C[C@@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(=O)O)C(=O)O)N BMVFXOQHDQZAQU-DCAQKATOSA-N 0.000 claims 2
WBRJVRXEGQIDRK-XIRDDKMYSA-N Leu-Trp-Ser Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](N)CC(C)C)C(=O)N[C@@H](CO)C(O)=O)=CNC2=C1 WBRJVRXEGQIDRK-XIRDDKMYSA-N 0.000 claims 2
WUGMRIBZSVSJNP-UHFFFAOYSA-N N-L-alanyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)C(N)C)C(O)=O)=CNC2=C1 WUGMRIBZSVSJNP-UHFFFAOYSA-N 0.000 claims 2
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108010002311 N-glycylglutamic acid Proteins 0.000 claims 2
BJEYSVHMGIJORT-NHCYSSNCSA-N Phe-Ala-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC1=CC=CC=C1 BJEYSVHMGIJORT-NHCYSSNCSA-N 0.000 claims 2
LDSOBEJVGGVWGD-DLOVCJGASA-N Phe-Asp-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CC1=CC=CC=C1 LDSOBEJVGGVWGD-DLOVCJGASA-N 0.000 claims 2
MJQFZGOIVBDIMZ-WHOFXGATSA-N Phe-Ile-Gly Chemical compound N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)O MJQFZGOIVBDIMZ-WHOFXGATSA-N 0.000 claims 2
WWPAHTZOWURIMR-ULQDDVLXSA-N Phe-Pro-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CC1=CC=CC=C1 WWPAHTZOWURIMR-ULQDDVLXSA-N 0.000 claims 2
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ZKQOUHVVXABNDG-IUCAKERBSA-N Pro-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1 ZKQOUHVVXABNDG-IUCAKERBSA-N 0.000 claims 2
GZFAWAQTEYDKII-YUMQZZPRSA-N Ser-Gly-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)CO GZFAWAQTEYDKII-YUMQZZPRSA-N 0.000 claims 2
ADJDNJCSPNFFPI-FXQIFTODSA-N Ser-Pro-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CO ADJDNJCSPNFFPI-FXQIFTODSA-N 0.000 claims 2
LUMXICQAOKVQOB-YWIQKCBGSA-N Thr-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@@H](N)[C@@H](C)O LUMXICQAOKVQOB-YWIQKCBGSA-N 0.000 claims 2
WYLAVUAWOUVUCA-XVSYOHENSA-N Thr-Phe-Asp Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(O)=O)C(O)=O WYLAVUAWOUVUCA-XVSYOHENSA-N 0.000 claims 2
ZOCJFNXUVSGBQI-HSHDSVGOSA-N Thr-Trp-Arg Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CNC2=CC=CC=C21)C(=O)N[C@@H](CCCN=C(N)N)C(=O)O)N)O ZOCJFNXUVSGBQI-HSHDSVGOSA-N 0.000 claims 2
CXPJPTFWKXNDKV-NUTKFTJISA-N Trp-Leu-Ala Chemical compound C1=CC=C2C(C[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(O)=O)=CNC2=C1 CXPJPTFWKXNDKV-NUTKFTJISA-N 0.000 claims 2
KDGFPPHLXCEQRN-STECZYCISA-N Tyr-Arg-Ile Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O KDGFPPHLXCEQRN-STECZYCISA-N 0.000 claims 2
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238000011218 seed culture Methods 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
238000002741 site-directed mutagenesis Methods 0.000 description 1
238000001542 size-exclusion chromatography Methods 0.000 description 1
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235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
150000007970 thio esters Chemical class 0.000 description 1
125000000341 threoninyl group Chemical group [H]OC([H])(C([H])([H])[H])C([H])(N([H])[H])C(*)=O 0.000 description 1
229960004072 thrombin Drugs 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
230000002103 transcriptional effect Effects 0.000 description 1
238000010361 transduction Methods 0.000 description 1
230000026683 transduction Effects 0.000 description 1
238000000844 transformation Methods 0.000 description 1
230000032258 transport Effects 0.000 description 1
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101150044170 trpE gene Proteins 0.000 description 1
108010050327 trypticase-soy broth Proteins 0.000 description 1
239000012137 tryptone Substances 0.000 description 1
125000002987 valine group Chemical group [H]N([H])C([H])(C(*)=O)C([H])(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
108700026220 vif Genes Proteins 0.000 description 1
239000000080 wetting agent Substances 0.000 description 1
210000005253 yeast cell Anatomy 0.000 description 1
NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
229960001763 zinc sulfate Drugs 0.000 description 1
229910000368 zinc sulfate Inorganic materials 0.000 description 1
RZLVQBNCHSJZPX-UHFFFAOYSA-L zinc sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Zn+2].[O-]S([O-])(=O)=O RZLVQBNCHSJZPX-UHFFFAOYSA-L 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
- C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
- C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
- C12P19/623—Avermectin; Milbemycin; Ivermectin; C-076

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Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Health & Medical Sciences (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Biomedical Technology (AREA)
General Chemical & Material Sciences (AREA)
Chemical Kinetics & Catalysis (AREA)
Molecular Biology (AREA)
Medicinal Chemistry (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Plant Pathology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)

OA1200200025A 1999-08-12 2000-07-24 Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins. OA11998A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US14864599P	1999-08-12	1999-08-12

Publications (1)

Publication Number	Publication Date
OA11998A true OA11998A (en)	2006-04-18

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ID=22526696

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Application Number	Title	Priority Date	Filing Date
OA1200200025A OA11998A (en)	1999-08-12	2000-07-24	Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins.

Country Status (39)

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US (3)	US6632673B1 (de)
EP (1)	EP1200606B1 (de)
JP (1)	JP3884651B2 (de)
KR (1)	KR100489856B1 (de)
CN (1)	CN1186446C (de)
AP (1)	AP2002002418A0 (de)
AR (1)	AR022646A1 (de)
AT (1)	ATE341632T1 (de)
AU (1)	AU778837B2 (de)
BG (1)	BG65902B1 (de)
BR (1)	BRPI0013119B8 (de)
CA (1)	CA2380872C (de)
CO (1)	CO5290295A1 (de)
CY (1)	CY1105778T1 (de)
CZ (1)	CZ304402B6 (de)
DE (1)	DE60031126T2 (de)
DK (1)	DK1200606T3 (de)
EA (1)	EA200200056A1 (de)
ES (1)	ES2272299T3 (de)
GT (1)	GT200000136A (de)
HK (1)	HK1046710B (de)
HR (1)	HRP20020130A2 (de)
HU (1)	HU230600B1 (de)
IL (2)	IL147563A0 (de)
IS (1)	IS6250A (de)
MA (1)	MA26813A1 (de)
MX (1)	MXPA02001513A (de)
NO (1)	NO20020664D0 (de)
NZ (1)	NZ516517A (de)
OA (1)	OA11998A (de)
PA (1)	PA8499501A1 (de)
PE (1)	PE20010690A1 (de)
PL (1)	PL203818B1 (de)
PT (1)	PT1200606E (de)
SK (1)	SK288086B6 (de)
TN (1)	TNSN00171A1 (de)
UY (1)	UY26286A1 (de)
WO (1)	WO2001012821A1 (de)
ZA (1)	ZA200201072B (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2001012821A1 (en) *	1999-08-12	2001-02-22	Pfizer Products Inc.	Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins
RS50813B (sr) *	2002-02-12	2010-08-31	Pfizer Products Inc.	Streptomyces avermitilis gen koji određuje odnos b2:b1 avermektina
US7943160B2 (en) *	2002-05-09	2011-05-17	Scimetrics Limited Corp.	Pest control methods
US9493744B2 (en) *	2012-06-20	2016-11-15	Genentech, Inc.	Methods for viral inactivation and other adventitious agents
KR102657859B1 (ko)	2021-04-07	2024-04-16	윤재식	일체형 내화 소켓

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IN167980B (de) *	1987-01-23	1991-01-19	Pfizer
US5525506A (en)	1987-01-23	1996-06-11	Pfizer Inc.	Process for production of avermectins and cultures therefor
US5238848A (en)	1987-01-23	1993-08-24	Pfizer Inc	Cultures for production of avermectins
US5234831A (en)	1987-01-23	1993-08-10	Pfizer Inc	Cultures for production of B avermectins
ATE89604T1 (de)	1987-10-23	1993-06-15	Pfizer	Verfahren zur herstellung von aglykonen von avermectin und sie enthaltende kulturen.
US5240850A (en)	1987-10-23	1993-08-31	Pfizer Inc.	Cultures for production of avermectin aglycones
US5252474A (en)	1989-03-31	1993-10-12	Merck & Co., Inc.	Cloning genes from Streptomyces avermitilis for avermectin biosynthesis and the methods for their use
DE69023036T2 (de)	1989-03-31	1996-06-13	Merck & Co Inc	Klonierung von Streptomyces avermitilis Genen zur Biosynthese von Avermectin und Verfahren zu ihrer Verwendung.
KR100221385B1 (ko)	1993-07-30	1999-10-01	디. 제이. 우드	스트렙토마이세스 아베르미틸리스로 부터의 측쇄 알파-케토산 데하이드로게나제 복합체를 암호화하는 유전자
FI942725A7 (fi)	1993-12-16	1995-06-17	Pfizer	Haaraketjuista alfaketohappodenydrogenaasikompleksia koodaavia geenejä Streptomyces avermitiliksesta
US5837458A (en)	1994-02-17	1998-11-17	Maxygen, Inc.	Methods and compositions for cellular and metabolic engineering
US6335160B1 (en)	1995-02-17	2002-01-01	Maxygen, Inc.	Methods and compositions for polypeptide engineering
US6117679A (en)	1994-02-17	2000-09-12	Maxygen, Inc.	Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5605793A (en)	1994-02-17	1997-02-25	Affymax Technologies N.V.	Methods for in vitro recombination
US6096548A (en)	1996-03-25	2000-08-01	Maxygen, Inc.	Method for directing evolution of a virus
CA2266423A1 (en)	1996-09-27	1998-04-02	Maxygen, Inc.	Methods for optimization of gene therapy by recursive sequence shuffling and selection
EP1717322B1 (de)	1997-01-17	2012-07-18	Codexis Mayflower Holdings, LLC	Evolution ganzer Zellen durch rekursive Sequenzrekombination
AU1449499A (en)	1997-10-31	1999-05-24	Maxygen, Incorporated	Modification of virus tropism and host range by viral genome shuffling
PL194270B1 (pl) *	1998-02-13	2007-05-31	Pfizer Prod Inc	Wyizolowana cząsteczka polinukleotydowa, cząsteczka oligonukleotydowa, zrekombinowany wektor, komórka gospodarza, wyizolowany produkt genu AveC S. avermitilis, wyizolowany produkt homologu genu AveC S. hygroscopicus, sposób wytwarzania zrekombinowanego produktu genu AveC, sposób wytwarzania zrekombinowanego produktu homologu genu AveC, cząsteczka polinukleotydowa, wektor do zastępowania genu, sposób identyfikowania mutacji otwartej ramki odczytuAveC, sposób wytwarzania nowych szczepów S. avermitilis, szczep S. avermitilis i sposób wytwarzaniaawermektyn
US6248579B1 (en) *	1998-02-13	2001-06-19	Pfizer Inc	Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
WO2001012821A1 (en) *	1999-08-12	2001-02-22	Pfizer Products Inc.	Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins

2000
- 2000-07-24 WO PCT/IB2000/001017 patent/WO2001012821A1/en not_active Ceased
- 2000-07-24 KR KR10-2002-7001807A patent/KR100489856B1/ko not_active Expired - Lifetime
- 2000-07-24 HU HU0202487A patent/HU230600B1/hu unknown
- 2000-07-24 ES ES00944154T patent/ES2272299T3/es not_active Expired - Lifetime
- 2000-07-24 PL PL353874A patent/PL203818B1/pl unknown
- 2000-07-24 HK HK02108367.6A patent/HK1046710B/zh unknown
- 2000-07-24 DE DE60031126T patent/DE60031126T2/de not_active Expired - Lifetime
- 2000-07-24 CZ CZ2002-296A patent/CZ304402B6/cs not_active IP Right Cessation
- 2000-07-24 OA OA1200200025A patent/OA11998A/en unknown
- 2000-07-24 AU AU58386/00A patent/AU778837B2/en not_active Expired
- 2000-07-24 BR BRPI0013119A patent/BRPI0013119B8/pt not_active IP Right Cessation
- 2000-07-24 EA EA200200056A patent/EA200200056A1/ru unknown
- 2000-07-24 AP APAP/P/2002/002418A patent/AP2002002418A0/en unknown
- 2000-07-24 AT AT00944154T patent/ATE341632T1/de active
- 2000-07-24 EP EP00944154A patent/EP1200606B1/de not_active Expired - Lifetime
- 2000-07-24 SK SK139-2002A patent/SK288086B6/sk not_active IP Right Cessation
- 2000-07-24 DK DK00944154T patent/DK1200606T3/da active
- 2000-07-24 PT PT00944154T patent/PT1200606E/pt unknown
- 2000-07-24 NZ NZ516517A patent/NZ516517A/en unknown
- 2000-07-24 JP JP2001516908A patent/JP3884651B2/ja not_active Expired - Lifetime
- 2000-07-24 CN CNB008117470A patent/CN1186446C/zh not_active Expired - Lifetime
- 2000-07-24 MX MXPA02001513A patent/MXPA02001513A/es active IP Right Grant
- 2000-07-24 CA CA002380872A patent/CA2380872C/en not_active Expired - Lifetime
- 2000-07-24 IL IL14756300A patent/IL147563A0/xx unknown
- 2000-07-24 HR HR20020130A patent/HRP20020130A2/hr not_active Application Discontinuation
- 2000-08-04 CO CO00058605A patent/CO5290295A1/es not_active Application Discontinuation
- 2000-08-10 US US09/637,040 patent/US6632673B1/en not_active Expired - Lifetime
- 2000-08-10 GT GT200000136A patent/GT200000136A/es unknown
- 2000-08-10 PE PE2000000812A patent/PE20010690A1/es not_active Application Discontinuation
- 2000-08-10 UY UY26286A patent/UY26286A1/es not_active Application Discontinuation
- 2000-08-10 PA PA20008499501A patent/PA8499501A1/es unknown
- 2000-08-10 AR ARP000104131A patent/AR022646A1/es active IP Right Grant
- 2000-08-11 TN TNTNSN00171A patent/TNSN00171A1/fr unknown
2002
- 2002-01-10 IL IL147563A patent/IL147563A/en active IP Right Grant
- 2002-01-25 IS IS6250A patent/IS6250A/is unknown
- 2002-02-07 ZA ZA200201072A patent/ZA200201072B/en unknown
- 2002-02-07 MA MA26513A patent/MA26813A1/fr unknown
- 2002-02-11 NO NO20020664A patent/NO20020664D0/no not_active Application Discontinuation
- 2002-03-05 BG BG106479A patent/BG65902B1/bg unknown
2003
- 2003-06-11 US US10/412,444 patent/US20040009560A1/en not_active Abandoned
- 2003-06-11 US US10/412,450 patent/US7858340B2/en not_active Expired - Fee Related
2006
- 2006-11-13 CY CY20061101647T patent/CY1105778T1/el unknown

Also Published As

Publication number	Publication date
US20030232415A1 (en)	2003-12-18
SK288086B6 (sk)	2013-06-03
PL203818B1 (pl)	2009-11-30
KR20020029379A (ko)	2002-04-18
ATE341632T1 (de)	2006-10-15
CN1186446C (zh)	2005-01-26
ES2272299T3 (es)	2007-05-01
MA26813A1 (fr)	2004-12-20
CA2380872C (en)	2007-11-27
CA2380872A1 (en)	2001-02-22
BG65902B1 (bg)	2010-04-30
EP1200606B1 (de)	2006-10-04
CY1105778T1 (el)	2011-02-02
US6632673B1 (en)	2003-10-14
CZ2002296A3 (cs)	2002-08-14
JP3884651B2 (ja)	2007-02-21
KR100489856B1 (ko)	2005-05-17
BRPI0013119B8 (pt)	2021-05-25
IL147563A (en)	2009-06-15
DK1200606T3 (da)	2007-01-08
IS6250A (is)	2002-01-25
HRP20020130A2 (en)	2003-06-30
UY26286A1 (es)	2001-03-16
EA200200056A1 (ru)	2002-06-27
WO2001012821A1 (en)	2001-02-22
HU230600B1 (hu)	2017-02-28
HUP0202487A3 (en)	2004-07-28
PT1200606E (pt)	2006-12-29
HUP0202487A2 (en)	2002-10-28
BR0013119B1 (pt)	2014-02-25
PA8499501A1 (es)	2001-12-14
EP1200606A1 (de)	2002-05-02
HK1046710A1 (en)	2003-01-24
PL353874A1 (en)	2003-12-01
CO5290295A1 (es)	2003-06-27
NZ516517A (en)	2003-10-31
SK1392002A3 (en)	2002-12-03
ZA200201072B (en)	2003-04-30
HK1046710B (zh)	2005-05-13
MXPA02001513A (es)	2002-07-02
NO20020664L (no)	2002-02-11
PE20010690A1 (es)	2001-07-06
DE60031126T2 (de)	2007-02-08
AP2002002418A0 (en)	2002-03-31
AU778837B2 (en)	2004-12-23
BR0013119A (pt)	2002-04-23
US7858340B2 (en)	2010-12-28
BG106479A (bg)	2002-10-31
CZ304402B6 (cs)	2014-04-16
DE60031126D1 (de)	2006-11-16
JP2003507017A (ja)	2003-02-25
IL147563A0 (en)	2002-08-14
GT200000136A (es)	2002-02-01
NO20020664D0 (no)	2002-02-11
AU5838600A (en)	2001-03-13
TNSN00171A1 (fr)	2005-11-10
US20040009560A1 (en)	2004-01-15
AR022646A1 (es)	2002-09-04
CN1373809A (zh)	2002-10-09

Publication	Publication Date	Title
US7029887B2 (en)	2006-04-18	Methods related to Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
AU752343B2 (en)	2002-09-19	Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins
CA2380872C (en)	2007-11-27	Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins
CA2475214C (en)	2012-07-31	Streptomyces avermitilis gene directing the ratio of b2:b1 avermectins
AU779569B2 (en)	2005-01-27	Streptomyces avermitilis gene directing the ratio of B2:B1 avermectins