PL2057274T3 - Fosfolipazy, kodujące je kwasy nukleinowe i sposoby ich wytwarzania i stosowania - Google Patents

Fosfolipazy, kodujące je kwasy nukleinowe i sposoby ich wytwarzania i stosowania

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Publication number
PL2057274T3
PL2057274T3 PL07853585T PL07853585T PL2057274T3 PL 2057274 T3 PL2057274 T3 PL 2057274T3 PL 07853585 T PL07853585 T PL 07853585T PL 07853585 T PL07853585 T PL 07853585T PL 2057274 T3 PL2057274 T3 PL 2057274T3
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PL
Poland
Prior art keywords
phospholipases
making
methods
nucleic acids
acids encoding
Prior art date
Application number
PL07853585T
Other languages
English (en)
Inventor
Eileen O'donoghue
Nelson R Barton
Original Assignee
Dsm Ip Assets Bv
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Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of PL2057274T3 publication Critical patent/PL2057274T3/pl

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409Fatty acids
    • C12P7/6418Fatty acids by hydrolysis of fatty acid esters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/6445Glycerides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/6445Glycerides
    • C12P7/6458Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/6445Glycerides
    • C12P7/6481Phosphoglycerides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436Fatty acid esters
    • C12P7/649Biodiesel, i.e. fatty acid alkyl esters
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Nutrition Science (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
PL07853585T 2006-09-21 2007-09-21 Fosfolipazy, kodujące je kwasy nukleinowe i sposoby ich wytwarzania i stosowania PL2057274T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US82651206P 2006-09-21 2006-09-21
EP07853585.3A EP2057274B1 (en) 2006-09-21 2007-09-21 Phospholipases, nucleic acids encoding them and methods for making and using them
PCT/US2007/079109 WO2008036863A2 (en) 2006-09-21 2007-09-21 Phospholipases, nucleic acids encoding them and methods for making and using them

Publications (1)

Publication Number Publication Date
PL2057274T3 true PL2057274T3 (pl) 2014-05-30

Family

ID=39201292

Family Applications (1)

Application Number Title Priority Date Filing Date
PL07853585T PL2057274T3 (pl) 2006-09-21 2007-09-21 Fosfolipazy, kodujące je kwasy nukleinowe i sposoby ich wytwarzania i stosowania

Country Status (13)

Country Link
US (1) US8637290B2 (pl)
EP (1) EP2057274B1 (pl)
KR (1) KR20090068266A (pl)
CN (1) CN101558154B (pl)
AR (1) AR062947A1 (pl)
BR (1) BRPI0716872A2 (pl)
CA (1) CA2663001A1 (pl)
EA (1) EA015591B1 (pl)
ES (1) ES2451266T3 (pl)
MX (1) MX2009003034A (pl)
PL (1) PL2057274T3 (pl)
PT (1) PT2057274E (pl)
WO (1) WO2008036863A2 (pl)

Families Citing this family (65)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2284897T3 (es) 2001-05-18 2007-11-16 Danisco A/S Procedimiento para la preparacion de una masa con una enzima.
US20080070291A1 (en) 2004-06-16 2008-03-20 David Lam Compositions and Methods for Enzymatic Decolorization of Chlorophyll
CN102533440B (zh) 2004-07-16 2017-09-19 杜邦营养生物科学有限公司 用酶使油脱胶的方法
WO2009081094A2 (en) 2007-12-21 2009-07-02 Danisco A/S Process
EP2246440A1 (en) * 2009-04-29 2010-11-03 Aterovax Enzymatic assay for the quantitative determination of phospholipase a1 or a2 activity in a sample
UA109884C2 (uk) * 2009-10-16 2015-10-26 Поліпептид, що має активність ферменту фосфатидилінозитол-специфічної фосфоліпази с, нуклеїнова кислота, що його кодує, та спосіб його виробництва і застосування
UA111708C2 (uk) 2009-10-16 2016-06-10 Бандж Ойлз, Інк. Спосіб рафінування олії
BR112013017319A2 (pt) 2011-01-21 2019-09-24 Novozymes As processo para produção de alquil ésteres de ácidos graxos
US20130014431A1 (en) * 2011-07-11 2013-01-17 Phillips 66 Company Advanced, biomass-derived, low-sulfur bunker fuels
KR101336785B1 (ko) * 2011-11-18 2013-12-04 한국생명공학연구원 갯벌 메타게놈 유래 신규 유전자 및 이로부터 얻어지는 포스포리파제 및 리파제 동시 활성을 보이는 신규 단백질
ES2676455T3 (es) 2012-02-17 2018-07-19 Clariant Produkte (Deutschland) Gmbh Procedimiento para la desmucilaginación enzimática de aceite
GB201208105D0 (en) * 2012-05-09 2012-06-20 Univ Dundee Modified plants
US10006041B2 (en) 2012-08-16 2018-06-26 Vib Vzw Means and methods for altering the lignin pathway in plants
RU2517538C1 (ru) * 2012-11-22 2014-05-27 Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) Фосфолипидный флуоресцентный зонд и тест-система для определения активности фосфолипазы а2 в сыворотке крови
RU2015126866A (ru) * 2012-12-11 2017-01-19 Новозимс А/С Полипептиды, обладающие активностью фосфолипазы с, и полинуклеотиды, кодирующие их
BR112015014339B1 (pt) * 2012-12-20 2021-08-10 Cargill, Incorporated Processo para a produção de um fluido dielétrico
US9828609B2 (en) 2013-03-12 2017-11-28 International Park Of Creativity Biological devices and methods for increasing the production of lycopene from plants
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
EP4331605A3 (en) 2013-09-13 2024-05-22 F. Hoffmann-La Roche AG Methods and compositions comprising purified recombinant polypeptides
ES2915378T3 (es) 2013-09-13 2022-06-22 Hoffmann La Roche Procedimientos para detectar y cuantificar una proteína de célula huésped en líneas celulares
CN104560910B (zh) * 2013-10-16 2021-03-26 丰益(上海)生物技术研发中心有限公司 提高毕赤酵母发酵产磷脂酶c产量的培养基及方法
CN104630175B (zh) * 2013-11-14 2019-10-25 丰益(上海)生物技术研发中心有限公司 一种磷脂酶c
EP2910129A1 (de) * 2014-02-21 2015-08-26 Clariant Produkte (Deutschland) GmbH Zusammensetzung für die enzymatische Ölentschleimung
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
CN106459935A (zh) * 2014-03-27 2017-02-22 诺维信公司 具有磷脂酶c活性的多肽和编码它们的多核苷酸
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
EP3282856A1 (en) 2015-04-14 2018-02-21 DSM IP Assets B.V. Use of phospholipase c
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
EP3359679A1 (en) 2015-10-09 2018-08-15 Novozymes A/S Enzymatic or non-enzymatic biodiesel polishing process
CA3014326C (en) 2016-02-19 2024-01-09 Basf Se Baking lipases
CN105567713B (zh) * 2016-03-09 2019-01-08 河北省农林科学院粮油作物研究所 花生AhPLDα3蛋白及其编码基因与应用
CN110218712B (zh) * 2016-06-02 2021-08-03 天津科技大学 磷脂酶d突变体及其在制备磷脂酸、磷脂酰丝氨酸中的应用
HUE070566T2 (hu) 2016-06-16 2025-06-28 Novozymes As Foszfolipidek csökkentése foszfolipidet tartalmazó olajos anyagban
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
BR112019019581A2 (pt) * 2017-03-20 2020-04-14 Novozymes As métodos para refinar um óleo vegetal e para produzir um polipeptídeo, uso de uma enzima, óleo vegetal refinado ou borra, polipeptídeo isolado ou purificado, composição, construto de ácido nucleico ou vetor de expressão, e, célula hospedeira recombinante
RU2019140871A (ru) 2017-05-12 2021-06-16 Басф Се Способ применения липазных ферментов для очистки
US12110531B2 (en) 2017-06-16 2024-10-08 Danstar Ferment Ag Method for increasing oil yield during ethanol production
CA3076578A1 (en) 2017-10-25 2019-05-02 Basf Se Beta-amylase enzymes
CN109321546B (zh) 2017-12-12 2021-08-31 丰益(上海)生物技术研发中心有限公司 磷脂酶c及其编码基因
US10258917B1 (en) 2018-02-09 2019-04-16 Tenneco Automotive Operating Company Inc. System for removing water and particulates from engine exhaust
CN108359654B (zh) * 2018-03-02 2021-11-05 江苏中酶生物科技有限公司 一种具有磷脂酶b活性的多肽或蛋白质及其应用
EP3769322A4 (en) 2018-03-21 2022-01-12 Cargill, Incorporated MINERAL OIL AND SYNTHETIC ESTER DIELECTRIC FLUIDS WITH INCREASED STABILITY
EP3776557B1 (en) * 2018-04-13 2025-01-29 The Hong Kong Polytechnic University Data storage using peptides
US11732250B2 (en) 2018-04-26 2023-08-22 Basf Se Lipase enzymes
US20210115422A1 (en) 2018-05-03 2021-04-22 Basf Se Amylase enzymes
CN109134645A (zh) * 2018-09-12 2019-01-04 深圳市菲鹏生物制药股份有限公司 一种细胞浓缩补料培养工艺
CN111378634B (zh) * 2018-12-28 2024-04-02 丰益(上海)生物技术研发中心有限公司 具有磷脂酶c活性的多肽及其用途
CN111051505B (zh) * 2018-12-29 2023-11-03 邦泰生物工程(深圳)有限公司 一种磷脂酶d突变体、其应用及其制备磷酯酰丝氨酸的方法
MX2021010109A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de un medio definido y una alimentacion de oligoelementos.
US20220186177A1 (en) 2019-02-20 2022-06-16 Basf Se Industrial fermentation process for bacillus using defined medium and magnesium feed
WO2020185737A1 (en) 2019-03-11 2020-09-17 Basf Se Amylases and methods for making and using them
WO2020193532A1 (en) 2019-03-25 2020-10-01 Basf Se Cleaning composition having amylase enzymes
US20220162576A1 (en) 2019-03-25 2022-05-26 Basf Se Amylase enzymes
US12606809B2 (en) 2019-03-25 2026-04-21 Basf Se Amylase enzymes
MX2021015364A (es) 2019-06-13 2022-01-24 Basf Se Metodo de recuperacion de una proteina de un caldo de fermentacion mediante el uso de un cation divalente.
PL3959326T3 (pl) 2019-07-05 2023-10-09 Basf Se Przemysłowy sposób fermentacji dla komórek bakteryjnych z wykorzystaniem kultury wstępnej okresowej z zasilaniem
CN114364795A (zh) 2019-08-22 2022-04-15 巴斯夫欧洲公司 淀粉酶变体
CN111004787B (zh) * 2020-01-06 2022-05-10 江南大学 一种链霉菌磷脂酶d突变体、改造方法及其应用
WO2022032011A1 (en) * 2020-08-06 2022-02-10 Poet Research, Inc. Endogenous lipase for metal reduction in distillers corn oil
CN112452277A (zh) * 2020-11-16 2021-03-09 西南石油大学 一种记忆效应影响下水合物生成实验装置
US20240218404A1 (en) 2021-05-04 2024-07-04 Novozymes A/S Enzymatic treatment of feedstock for hvo production
CN120640986A (zh) 2023-02-24 2025-09-12 卡拉万成分股份有限公司 蛋类替代物组合物及其制备和使用方法
WO2024243330A1 (en) 2023-05-22 2024-11-28 Caravan Ingredients Inc. Baked goods enhancer and methods of making and using the same
CN117187211B (zh) * 2023-08-04 2025-04-01 华南理工大学 一种磷脂酶c晶体及磷脂酶c突变体的制备方法与应用

Family Cites Families (156)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE318048B (pl) 1959-09-21 1969-12-01 Pellerin Ab Zenith
GB1541017A (en) 1975-03-10 1979-02-21 Unilever Ltd Degumming process for triglyceride oils
SE417441B (sv) 1977-08-19 1981-03-16 Kare Larsson Forfarande for att isolera polera lipider ur en blandning av polera och opolera cerealiclipider genom blandning med vatten och derpa foljande tyngdkraftsseparation
US4240972A (en) 1978-12-19 1980-12-23 Canada Packers Limited Continuous process for contacting of triglyceride oils with _an acid
US5366558A (en) 1979-03-23 1994-11-22 Brink David L Method of treating biomass material
WO1981000857A1 (fr) 1979-09-28 1981-04-02 Vsesoyuzny Ni Biotek Inst Procede de production d'alcool ethylique a partir d'un materiau d'amidon brut
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4707364A (en) 1984-01-27 1987-11-17 Miles Laboratories, Inc. Composition for accelerating cheese aging
US5087571A (en) 1984-06-22 1992-02-11 President And Fellows Of Harvard College Method for providing a cell culture from a transgenic non-human mammal
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
GB2183661B (en) 1985-03-30 1989-06-28 Marc Ballivet Method for obtaining dna, rna, peptides, polypeptides or proteins by means of a dna recombinant technique
US4752483A (en) 1986-05-30 1988-06-21 Campbell Soup Company Method for producing a highly flavored cheese ingredient
US5030240A (en) 1986-06-09 1991-07-09 The Clorox Company Enzymatic peracid bleaching system
US4946778A (en) 1987-09-21 1990-08-07 Genex Corporation Single polypeptide chain binding molecules
US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US5015580A (en) 1987-07-29 1991-05-14 Agracetus Particle-mediated transformation of soybean plants and lines
US5573933A (en) 1987-04-14 1996-11-12 Luminis Pty, Ltd. Transgenic pigs
US6054270A (en) 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US5217879A (en) 1989-01-12 1993-06-08 Washington University Infectious Sindbis virus vectors
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5527681A (en) 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5288619A (en) 1989-12-18 1994-02-22 Kraft General Foods, Inc. Enzymatic method for preparing transesterified oils
WO1991016427A1 (en) 1990-04-24 1991-10-31 Stratagene Methods for phenotype creation from multiple gene populations
US5326477A (en) 1990-05-07 1994-07-05 Bio-Sep, Inc. Process for digesting solid waste
US5387742A (en) 1990-06-15 1995-02-07 Scios Nova Inc. Transgenic mice displaying the amyloid-forming pathology of alzheimer's disease
DE69133512T2 (de) 1990-11-23 2006-09-28 Bayer Bioscience N.V. Verfahren zur Transformation monokotyler Pflanzen
EP0563296B1 (en) 1990-12-20 1999-03-17 Ixsys, Inc. Optimization of binding proteins
US6110700A (en) 1991-03-11 2000-08-29 The General Hospital Corporation PRAD1 cyclin and its cDNA
DE4115938A1 (de) 1991-05-16 1992-11-19 Metallgesellschaft Ag Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen
US5922854A (en) 1991-06-14 1999-07-13 Kumar; Ramesh Purifying Human Hemogloblin from transgenic pig red cells and plasmids containing pig globin nucleic acids
CA2075135A1 (en) 1991-08-02 1993-02-03 David E. Ellis Particle-mediated transformation of gymnosperms
US5632957A (en) 1993-11-01 1997-05-27 Nanogen Molecular biological diagnostic systems including electrodes
JP2626380B2 (ja) 1991-11-12 1997-07-02 株式会社大林組 クレーン用吊治具の旋回防止装置
JPH05236997A (ja) 1992-02-28 1993-09-17 Hitachi Ltd ポリヌクレオチド捕捉用チップ
JP2937746B2 (ja) 1993-04-25 1999-08-23 昭和産業株式会社 油脂の精製方法
JPH06306386A (ja) 1993-04-25 1994-11-01 Showa Sangyo Co Ltd 油脂の精製方法
EP0670670A4 (en) 1993-09-30 1996-04-24 Agracetus HETEROLOGICAL PEROXIDASE PROCESSING TRANSGENIC COTTON PLANTS.
US6045996A (en) 1993-10-26 2000-04-04 Affymetrix, Inc. Hybridization assays on oligonucleotide arrays
US6468742B2 (en) 1993-11-01 2002-10-22 Nanogen, Inc. Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip
US5965452A (en) 1996-07-09 1999-10-12 Nanogen, Inc. Multiplexed active biologic array
DE4339556C1 (de) 1993-11-19 1995-02-02 Metallgesellschaft Ag Verfahren zum Entschleimen von Pflanzenöl mittels Enzymen
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5639940A (en) 1994-03-03 1997-06-17 Pharmaceutical Proteins Ltd. Production of fibrinogen in transgenic animals
MX198456B (es) 1994-03-09 2000-09-05 Abbott Lab Animales trangenicos que producen oligosacaridos y glucoconjugados.
WO1995024494A1 (en) 1994-03-09 1995-09-14 Abbott Laboratories Humanized milk
DK0753057T3 (da) 1994-03-29 2006-01-30 Novozymes As Alkalisk Bacillus-amylase
US5807522A (en) 1994-06-17 1998-09-15 The Board Of Trustees Of The Leland Stanford Junior University Methods for fabricating microarrays of biological samples
US5959171A (en) 1994-08-17 1999-09-28 Pharming B.V. Method for the production of biologically active polypeptides in a mammal's
EP0778886A4 (en) 1994-09-01 2001-05-02 TRANSGENIC ANIMAL WHICH EXPRESSES A FAMILY FORM OF THE HUMAN AMYLOID FOREQUARTER PROTEIN
US5795737A (en) 1994-09-19 1998-08-18 The General Hospital Corporation High level expression of proteins
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
US5880327A (en) 1994-09-21 1999-03-09 American National Red Cross Transgenic mammals expressing human coagulation factor VIII
US5556752A (en) 1994-10-24 1996-09-17 Affymetrix, Inc. Surface-bound, unimolecular, double-stranded DNA
US6187992B1 (en) 1994-12-05 2001-02-13 Merck & Co., Inc. Transgenic mouse having a disrupted amyloid precursor protein gene
US5830645A (en) 1994-12-09 1998-11-03 The Regents Of The University Of California Comparative fluorescence hybridization to nucleic acid arrays
US5705369A (en) 1994-12-27 1998-01-06 Midwest Research Institute Prehydrolysis of lignocellulose
US6093562A (en) 1996-02-05 2000-07-25 Novo Nordisk A/S Amylase variants
US5959098A (en) 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
CA2223103A1 (en) 1995-06-06 1996-12-12 Isis Pharmaceuticals Inc. Oligonucleotides having phosphorothioate linkages of high chiral purity
BR9608589A (pt) 1995-06-07 1999-09-14 Willian C Orr Composições e processo de combustão em fase vapor
RU2182163C2 (ru) * 1995-06-07 2002-05-10 Уильям К. Орр Состав топлива
TR199701705T1 (xx) 1995-06-27 1998-04-21 Unilever N.V. Trigliserid ya�lar�n i�lenmesinde hareketsiz enzim ve kullan�m�.
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5985662A (en) 1995-07-13 1999-11-16 Isis Pharmaceuticals Inc. Antisense inhibition of hepatitis B virus replication
US5958672A (en) 1995-07-18 1999-09-28 Diversa Corporation Protein activity screening of clones having DNA from uncultivated microorganisms
DE19527274A1 (de) 1995-07-26 1997-01-30 Metallgesellschaft Ag Enzymatisches Verfahren zur Entschleimung von pflanzlichen Ölen mit Aspergillus-Phospholipase
SE504664C2 (sv) 1995-09-22 1997-03-24 Scotia Lipidteknik Ab Sätt att framställa fraktionerad olja, oljan, dess användning samt emulsionskomposition innehållande oljan
US5721118A (en) 1995-10-31 1998-02-24 The Regents Of The University Of California, San Diego Mammalian artificial chromosomes and methods of using same
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6171820B1 (en) 1995-12-07 2001-01-09 Diversa Corporation Saturation mutagenesis in directed evolution
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US6238884B1 (en) 1995-12-07 2001-05-29 Diversa Corporation End selection in directed evolution
US6022963A (en) 1995-12-15 2000-02-08 Affymetrix, Inc. Synthesis of oligonucleotide arrays using photocleavable protecting groups
US6013440A (en) 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US6096548A (en) 1996-03-25 2000-08-01 Maxygen, Inc. Method for directing evolution of a virus
US6025155A (en) 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US5697987A (en) 1996-05-10 1997-12-16 The Trustees Of Princeton University Alternative fuel
US6197070B1 (en) 1996-05-15 2001-03-06 The Procter & Gamble Company Detergent compositions comprising alpha combination of α-amylases for malodor stripping
AU3568897A (en) 1996-06-07 1998-01-05 Eos Biotechnology, Inc. Immobilised linear oligonucleotide arrays
US6326341B1 (en) 1996-09-11 2001-12-04 The Procter & Gamble Company Low foaming automatic dishwashing compositions
AU4772597A (en) 1996-10-31 1998-05-22 Novo Nordisk A/S Novel phospholipase, production and use thereof
US6127137A (en) 1996-10-31 2000-10-03 Novo Nordisk A/S Acidic phospholipase, production and methods using thereof
US6103505A (en) 1996-12-09 2000-08-15 Novo Nordisk A/S Method for reducing phosphorus content of edible oils
AU5187898A (en) 1996-12-09 1998-07-03 Novo Nordisk A/S Reduction of phosphorus containing components in edible oils comprising a high amount of non-hydratable phosphorus by use of a phospholipase, a phospholipase from a filamentous fungus having phospholipase a and/or b activity
JP4017082B2 (ja) 1996-12-19 2007-12-05 ユニリーバー・ナームローゼ・ベンノートシャープ 固定化酵素及びトリグリセリド油の処理のためのその使用
US6069122A (en) 1997-06-16 2000-05-30 The Procter & Gamble Company Dishwashing detergent compositions containing organic diamines for improved grease cleaning, sudsing, low temperature stability and dissolution
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
EP1717322B1 (en) 1997-01-17 2012-07-18 Codexis Mayflower Holdings, LLC Evolution of whole cells and organisms by recursive sequence recombination
DE19703364A1 (de) 1997-01-30 1998-08-06 Henkel Ecolab Gmbh & Co Ohg Pastenförmiges Wasch- und Reinigungsmittel
US6303803B1 (en) 1997-01-31 2001-10-16 Cargill, Incorporated Removal of sterols from fats and oils
US5880300A (en) 1997-01-31 1999-03-09 Cargill, Incorporated Phospholipid-based removal of sterols from fats and oils
JP4263248B2 (ja) 1997-03-18 2009-05-13 ノボザイムス アクティーゼルスカブ Dnaのシャッフリングによるライブラリーの作成方法
EP0973940B1 (en) 1997-03-18 2008-07-02 Novozymes A/S An in vitro method for the construction of a dna library
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6153410A (en) 1997-03-25 2000-11-28 California Institute Of Technology Recombination of polynucleotide sequences using random or defined primers
US6333181B1 (en) 1997-04-07 2001-12-25 University Of Florida Research Foundation, Inc. Ethanol production from lignocellulose
US5916780A (en) 1997-06-09 1999-06-29 Iogen Corporation Pretreatment process for conversion of cellulose to fuel ethanol
US6826296B2 (en) 1997-07-25 2004-11-30 Affymetrix, Inc. Method and system for providing a probe array chip design database
CN1273609A (zh) 1997-08-15 2000-11-15 希斯克有限公司 检测或量化核酸物类的方法和组合物
CN1677076A (zh) 1997-09-11 2005-10-05 生物风险公司 制备高密度阵列的方法
US6465178B2 (en) 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
AU1124499A (en) 1997-10-28 1999-05-17 Maxygen, Inc. Human papillomavirus vectors
CA2308119C (en) 1997-10-30 2014-06-03 Novo Nordisk A/S .alpha.-amylase mutants
WO1999023107A1 (en) 1997-10-31 1999-05-14 Maxygen, Incorporated Modification of virus tropism and host range by viral genome shuffling
EP1032644B1 (en) 1997-11-10 2003-03-19 The Procter & Gamble Company Process for preparing a detergent tablet
US6118044A (en) 1997-11-14 2000-09-12 Sankyo Company, Limited Transgenic animal allergy models and methods for their use
DK1036198T3 (da) 1997-12-08 2013-01-02 California Inst Of Techn Fremgangsmåde til fremstilling af polynukleotid- og polypeptidsekvenser
US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
CA2320958A1 (en) 1998-02-11 1999-08-19 Maxygen, Inc. Antigen library immunization
MXPA00007893A (es) 1998-02-11 2002-10-23 Maxygen Inc Direccion de vectores de vacunas geneticas.
AU3463699A (en) 1998-04-03 1999-10-25 Phylos, Inc. Addressable protein arrays
US6156952A (en) 1998-04-09 2000-12-05 Constituent Institution Of The University Of Maryland System HIV transgenic animals and uses therefor
AU3408199A (en) 1998-05-01 1999-11-23 Novo Nordisk A/S Enhancers such as n-hydroxyacetanilide
US6048695A (en) 1998-05-04 2000-04-11 Baylor College Of Medicine Chemically modified nucleic acids and methods for coupling nucleic acids to solid support
DE19824705A1 (de) 1998-06-03 1999-12-09 Henkel Kgaa Amylase und Protease enthaltende Wasch- und Reinigungsmittel
US6022567A (en) 1998-06-23 2000-02-08 Nestec S.A. Flavor enhancer
ATE547532T1 (de) 1998-06-29 2012-03-15 Bristol Myers Squibb Co Verfahren zur erzeugung von hochgradig diversen bibliotheken
JP4335995B2 (ja) 1998-07-22 2009-09-30 昭 神谷 環境保全型粒状洗浄用組成物
FR2782323B1 (fr) 1998-08-12 2002-01-11 Proteus Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues
US6183962B1 (en) 1998-09-09 2001-02-06 Millennium Pharmaceuticals, Inc. Protein kinase molecules and uses therefor
US6277628B1 (en) 1998-10-02 2001-08-21 Incyte Genomics, Inc. Linear microarrays
US6172248B1 (en) 1998-11-20 2001-01-09 Ip Holdings, L.L.C. Methods for refining vegetable oils and byproducts thereof
US6277489B1 (en) 1998-12-04 2001-08-21 The Regents Of The University Of California Support for high performance affinity chromatography and other uses
FR2788782B1 (fr) 1999-01-25 2003-01-31 Gie Agro Ind Produit multienzymatique a activites glucoamylasique, proteolytique et xylanasique et procede pour sa production par fermentation a l'etat solide de son de ble avec aspergillus niger
US6617122B1 (en) 1999-03-15 2003-09-09 Xenon Genetics, Inc. Process for identifying modulators of ABC1 activity
AU766617B2 (en) 1999-03-16 2003-10-23 Novozymes A/S Process for producing cheese
US6399121B1 (en) 1999-03-16 2002-06-04 Novozymes A/S Process for producing cheese
US6511824B1 (en) 1999-03-17 2003-01-28 Exelixis, Inc. Nucleic acids and polypeptides of invertebrate TWIK channels and methods of use
US6309871B1 (en) 1999-03-31 2001-10-30 Novozymes A/S Polypeptides having alkaline α-amylase activity
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6653151B2 (en) 1999-07-30 2003-11-25 Large Scale Proteomics Corporation Dry deposition of materials for microarrays using matrix displacement
US6409841B1 (en) 1999-11-02 2002-06-25 Waste Energy Integrated Systems, Llc. Process for the production of organic products from diverse biomass sources
EP1106603A3 (en) 1999-12-06 2003-11-19 Fuji Photo Film Co., Ltd. DNA chip and reactive solid carrier
US6531644B1 (en) 2000-01-14 2003-03-11 Exelixis, Inc. Methods for identifying anti-cancer drug targets
ES2166316B1 (es) 2000-02-24 2003-02-16 Ct Investig Energeticas Ciemat Procedimiento de produccion de etanol a partir de biomasa lignocelulosica utilizando una nueva levadura termotolerante.
AU2001291685A1 (en) * 2000-07-31 2002-02-13 Bayer Aktiengesellschaft Regulation of human phosphatidylinositol-specific phospholipase c-like enzyme
US6423145B1 (en) 2000-08-09 2002-07-23 Midwest Research Institute Dilute acid/metal salt hydrolysis of lignocellulosics
JP2004518413A (ja) 2000-10-12 2004-06-24 エクセリクシス・インコーポレイテッド ヒトect2と使用方法
US6309872B1 (en) 2000-11-01 2001-10-30 Novozymes Biotech, Inc Polypeptides having glucoamylase activity and nucleic acids encoding same
US6660491B2 (en) * 2000-11-24 2003-12-09 Ikeda Food Research Co., Ltd. Process for producing dietary sterol fatty acid esters
JP2005517418A (ja) 2002-02-20 2005-06-16 ノボザイムス アクティーゼルスカブ チーズの製造方法
US7226771B2 (en) 2002-04-19 2007-06-05 Diversa Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
AU2003243157C1 (en) * 2002-04-19 2008-09-04 Verenium Corporation Phospholipases, nucleic acids encoding them and methods for making and using them
EP2853593B1 (en) 2003-03-07 2017-10-04 DSM IP Assets B.V. Hydrolases, nucleic acids encoding them and mehods for making and using them
US20060014260A1 (en) 2004-05-07 2006-01-19 Zhiliang Fan Lower cellulase requirements for biomass cellulose hydrolysis and fermentation
CA2601472A1 (en) 2005-03-08 2006-09-14 Verenium Corporation Hydrolases, nucleic acids encoding them and methods for improving paper strength
US9409174B2 (en) 2013-06-21 2016-08-09 Bio-Rad Laboratories, Inc. Microfluidic system with fluid pickups

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CA2663001A1 (en) 2008-03-27
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