PL3033184T3 - Selektywne dostarczanie materiału do komórek - Google Patents

Selektywne dostarczanie materiału do komórek

Info

Publication number
PL3033184T3
PL3033184T3 PL14836593T PL14836593T PL3033184T3 PL 3033184 T3 PL3033184 T3 PL 3033184T3 PL 14836593 T PL14836593 T PL 14836593T PL 14836593 T PL14836593 T PL 14836593T PL 3033184 T3 PL3033184 T3 PL 3033184T3
Authority
PL
Poland
Prior art keywords
cells
selective delivery
selective
delivery
Prior art date
Application number
PL14836593T
Other languages
English (en)
Inventor
Armon R. Sharei
Viktor A. Adalsteinsson
Nahyun CHO
Robert S. Langer
J. Christopher Love
Klavs F. Jensen
Original Assignee
Massachusetts Institute Of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Massachusetts Institute Of Technology filed Critical Massachusetts Institute Of Technology
Publication of PL3033184T3 publication Critical patent/PL3033184T3/pl

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00Nanotechnology for materials or surface science, e.g. nanocomposites
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0693Tumour cells; Cancer cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484Optical investigation techniques, e.g. flow cytometry microstructural devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652Sorting or classification of particles or molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0475Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/16Microfluidic devices; Capillary tubes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1006Investigating individual particles for cytology
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1028Sorting particles

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Nanotechnology (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Fluid Mechanics (AREA)
  • Clinical Laboratory Science (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Composite Materials (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Plant Pathology (AREA)
PL14836593T 2013-08-16 2014-08-15 Selektywne dostarczanie materiału do komórek PL3033184T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361866972P 2013-08-16 2013-08-16
EP14836593.5A EP3033184B1 (en) 2013-08-16 2014-08-15 Selective delivery of material to cells
PCT/US2014/051343 WO2015023982A1 (en) 2013-08-16 2014-08-15 Selective delivery of material to cells

Publications (1)

Publication Number Publication Date
PL3033184T3 true PL3033184T3 (pl) 2021-09-20

Family

ID=52468732

Family Applications (1)

Application Number Title Priority Date Filing Date
PL14836593T PL3033184T3 (pl) 2013-08-16 2014-08-15 Selektywne dostarczanie materiału do komórek

Country Status (13)

Country Link
US (3) US10124336B2 (pl)
EP (2) EP3033184B1 (pl)
JP (1) JP6502940B2 (pl)
KR (4) KR102243597B1 (pl)
CN (1) CN105848793B (pl)
AU (2) AU2014306423B2 (pl)
CA (1) CA2921579C (pl)
ES (1) ES2865107T3 (pl)
IL (1) IL244113B (pl)
MX (2) MX382654B (pl)
PL (1) PL3033184T3 (pl)
SG (2) SG10201801188XA (pl)
WO (1) WO2015023982A1 (pl)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT2768942T (pt) * 2011-10-17 2020-01-21 Massachusetts Inst Technology Entrega intracelular
KR102243597B1 (ko) 2013-08-16 2021-04-26 메사추세츠 인스티튜트 오브 테크놀로지 세포로의 선택적 물질 전달
KR20170074235A (ko) 2014-10-31 2017-06-29 메사추세츠 인스티튜트 오브 테크놀로지 면역 세포로의 생체분자의 전달
CN107002089B (zh) * 2014-11-14 2021-09-07 麻省理工学院 化合物和组合物向细胞中的破坏和场实现的递送
CN107250373A (zh) 2015-01-12 2017-10-13 麻省理工学院 通过微流体递送实现的基因编辑
AU2016289530B2 (en) 2015-07-09 2021-05-06 Massachusetts Institute Of Technology Delivery of materials to anucleate cells
EP3344575B1 (en) 2015-09-04 2020-04-15 SQZ Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
PL3344747T3 (pl) 2015-09-04 2023-06-19 Sqz Biotechnologies Company Wewnątrzkomórkowe dostarczanie biocząsteczek za pośrednictwem powierzchni z porami
US20180327706A1 (en) * 2015-10-19 2018-11-15 The Methodist Hospital Crispr-cas9 delivery to hard-to-transfect cells via membrane deformation
MX2018007987A (es) 2015-12-28 2019-01-10 Novartis Ag Composiciones y metodos para el tratamiento de hemoglobinopatias.
JP7033535B2 (ja) 2016-01-12 2022-03-10 スクイーズ バイオテクノロジーズ カンパニー 複合体の細胞内送達
WO2017173373A1 (en) 2016-03-31 2017-10-05 Massachusetts Institute Of Technology Flow-through microfluidic methods and devices featuring membrane-perturbing surface interactions for intracellular delivery
AU2017259988B2 (en) 2016-05-03 2023-04-27 Sqz Biotechnologies Company Intracellular delivery of biomolecules to induce tolerance
CA3023092A1 (en) * 2016-05-03 2017-11-09 Sqz Biotechnologies Company Intracellular delivery of biomolecules to induce tolerance
CN106378213B (zh) * 2016-05-05 2018-08-17 海南大学 基于介电泳的可变形微颗粒分离芯片
US11999931B2 (en) 2016-08-20 2024-06-04 The Regents Of The University Of California High-throughput system and method for the temporary permeabilization of cells
WO2018089497A1 (en) * 2016-11-08 2018-05-17 Georgia Tech Research Corporation Methods for convectively-driven intracellular delivery
TW201839136A (zh) 2017-02-06 2018-11-01 瑞士商諾華公司 治療血色素異常症之組合物及方法
US11383241B2 (en) * 2017-10-11 2022-07-12 The Regents Of The University Of California Mechano-node pore sensing
WO2019089034A1 (en) * 2017-11-02 2019-05-09 The Methodist Hospital Crispr-cas9 delivery to hard-to-transfect cells via membrane deformation
EP3720878A1 (en) 2017-12-05 2020-10-14 SQZ Biotechnologies Company Intracellular delivery of biomolecules to modulate antibody production
US11365390B2 (en) 2017-12-19 2022-06-21 Xcell Biosciences, Inc. Methods of modulating cell phenotype by way of regulating the gaseous environment
EP3728559A1 (en) 2017-12-20 2020-10-28 SQZ Biotechnologies Company System for delivery of a payload into a cell
AU2019234549B2 (en) 2018-03-12 2026-02-12 Sqz Biotechnologies Company Methods for treating HPV-associated diseases
JP2019154314A (ja) * 2018-03-13 2019-09-19 国立大学法人九州大学 キャピラリーチップ及びこれを備える装置、並びに細胞への外来物質の導入方法
EP3556845A1 (en) 2018-04-20 2019-10-23 Cellix Limited A method and device for transfecting cells
CN109351370B (zh) * 2018-11-21 2020-05-05 晶准生物医学(深圳)有限公司 微流控芯片和细胞筛选方法
WO2020154696A1 (en) 2019-01-25 2020-07-30 Sqz Biotechnologies Company Anucleate cell-derived vaccines
CA3131701A1 (en) 2019-02-28 2020-09-03 Sqz Biotechnologies Company Delivery of biomolecules to pbmcs to modify an immune response
DE102019108155B3 (de) 2019-03-29 2020-06-04 Leibniz-Institut Für Photonische Technologien E.V. Mikrotropfenrückhalteanordnung
KR20210149791A (ko) 2019-04-08 2021-12-09 에스큐지 바이오테크놀로지스 컴퍼니 페이로드를 세포 내로 전달하기 위한 시스템에서 이용하기 위한 카트리지
US12465912B2 (en) 2019-04-11 2025-11-11 The Chinese University Of Hong Kong Systems and methods for controlled membrane disruption
US20220213422A1 (en) * 2019-05-15 2022-07-07 Cellfe, Inc. Methods and systems for intracellular delivery and products thereof
WO2020252215A1 (en) 2019-06-12 2020-12-17 Cellfe, Inc. Methods and systems for cell labeling and imaging
JP7846007B2 (ja) 2019-12-18 2026-04-14 ノバルティス アーゲー 異常ヘモグロビン症の治療用組成物及び方法
CN111413257B (zh) * 2020-01-21 2021-05-14 中国科学院电子学研究所 细胞核电学性能检测装置及方法
JP2023527690A (ja) 2020-05-11 2023-06-30 エフ・ホフマン-ラ・ロシュ・アクチェンゲゼルシャフト 改変pbmcと免疫抱合体との併用療法
US20210371881A1 (en) * 2020-05-26 2021-12-02 TransCytos, LLC Devices and methods for transfection
US20250255961A1 (en) 2020-07-29 2025-08-14 Stemcell Technologies Canada Inc. Methods to stimulate immune responses to mutant ras using nucleated cells
KR20230058388A (ko) 2020-07-29 2023-05-03 에스큐지 바이오테크놀로지스 컴퍼니 무핵 세포를 사용해 돌연변이체 ras에 대한 면역 반응을 자극하는 방법
CA3201944A1 (en) 2020-11-18 2022-05-27 Cellfe, Inc. Methods and systems for mechanoporation-based payload delivery into biological cells
US12227729B2 (en) 2020-12-24 2025-02-18 Cellfe, Inc. Methods and systems for high-throughput cell processing
US12018240B2 (en) 2020-12-29 2024-06-25 Stemcell Technologies Canada Inc. High-throughput microfluidic chip having parallelized constrictions for perturbing cell membranes
EP4271793A1 (en) 2020-12-29 2023-11-08 SQZ Biotechnologies Company Microfluidic chip having increased throughput for use in a system for delivery of a payload into a cell
EP4271407A1 (en) 2020-12-29 2023-11-08 SQZ Biotechnologies Company Formulations of activating antigen carriers
US20220296691A1 (en) 2020-12-29 2022-09-22 Sqz Biotechnologies Company Methods for treating cancer with activating antigen carriers
EP4271711A1 (en) 2020-12-29 2023-11-08 SQZ Biotechnologies Company Methods for treating cancers with modified pbmcs
US12150982B2 (en) 2020-12-29 2024-11-26 Stemcell Technologies Canada Inc. Formulations of PBMCs
US20250127809A1 (en) 2021-06-23 2025-04-24 Novartis Ag Compositions and methods for the treatment of hemoglobinopathies
US20250327027A1 (en) 2021-07-29 2025-10-23 Stemcell Technologies Canada Inc. Methods to generate enhanced tumor infiltrating lymphocytes through microfluidic delivery
JP2024545582A (ja) 2021-11-11 2024-12-10 ステムセル テクノロジーズ カナダ インコーポレイテッド マイクロ流体送達による増強された腫瘍浸潤リンパ球を作製する方法
AU2023277543A1 (en) 2022-05-27 2024-12-19 Stemcell Technologies Canada Inc. Cartridges and devices for use in a system for delivery of a payload into a cell
EP4561620A1 (en) 2022-07-28 2025-06-04 Stemcell Technologies Canada Inc. Methods for treating cancer with enhanced antigen presenting cells
WO2024026491A2 (en) 2022-07-28 2024-02-01 Sqz Biotechnologies Company Enhanced antigen presenting cell formulations

Family Cites Families (123)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2502621C3 (de) 1975-01-23 1978-09-14 Kernforschungsanlage Juelich Gmbh, 5170 Juelich Messung elastischer und dielektrischer Eigenschaften der Membran lebender Zellen
US4376634A (en) 1980-05-30 1983-03-15 Mallinckrodt, Inc. Assay kit having syringe, dilution device and reagents within sealed container
US4478824A (en) 1983-08-08 1984-10-23 Franco Robert S Method for altering red blood cell function and survival
FR2569477B1 (fr) 1984-08-24 1987-01-02 Descartes Universite Rene Appareil et procede pour la determination de la deformabilite des globules rouges du sang
JP2720161B2 (ja) 1988-02-01 1998-02-25 株式会社アドバンス 細胞変形能測定装置
JP2685544B2 (ja) 1988-11-11 1997-12-03 株式会社日立製作所 血液フィルタおよび血液検査方法並びに血液検査装置
JP2532707B2 (ja) 1990-03-08 1996-09-11 佑二 菊池 血液回路及びこれを用いた血液測定装置及び血液測定方法
US5643577A (en) 1990-04-24 1997-07-01 The University Of Newcastle Research Associates Limited Oral vaccine comprising antigen surface-associated with red blood cells
US5658892A (en) 1993-01-15 1997-08-19 The General Hospital Corporation Compound delivery using high-pressure impulse transients
US6218166B1 (en) 1994-12-09 2001-04-17 John Wayne Cancer Institute Adjuvant incorporation into antigen carrying cells: compositions and methods
CA2214649C (en) 1995-03-08 2007-06-12 Zeling Cai Antigen presenting system and methods for activation of t-cells
US6051409A (en) 1995-09-25 2000-04-18 Novartis Finance Corporation Method for achieving integration of exogenous DNA delivered by non-biological means to plant cells
US6133503A (en) 1995-10-31 2000-10-17 The Regents Of The University Of California Mammalian artificial chromosomes and methods of using same
AU725669B2 (en) 1995-12-04 2000-10-19 Puget Sound Blood Center Non-immunogenic and toleragenic platelet and red blood cell compositions
US5951976A (en) 1996-03-28 1999-09-14 Whitenead Institute For Biomedical Research Opsonin-enhanced cells, and methods of modulating an immune response to an antigen
US5885470A (en) 1997-04-14 1999-03-23 Caliper Technologies Corporation Controlled fluid transport in microfabricated polymeric substrates
EP0882448B1 (en) 1997-05-05 2005-01-12 DIDECO S.r.l. Method of encapsulating biologically active agents within erythrocytes and apparatus therefor
US5842787A (en) 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
SE9704076D0 (sv) 1997-11-06 1997-11-06 Holdingbolaget Vid Goeteborgs Method for permeabilisation of cell structures and use thereof
GB9816583D0 (en) 1998-07-31 1998-09-30 Univ Ulster Nucleic acid carrier
WO2000078920A1 (en) 1999-06-21 2000-12-28 The General Hospital Corporation Methods and devices for cell culturing and organ assist systems
JP2002325572A (ja) 2000-12-25 2002-11-12 Univ Osaka 外来物質の導入方法
AU2002252073A1 (en) 2001-02-22 2002-09-12 The Scepens Eye Research Institute, Inc. Tolerogenic antigen presenting cells and in treating immune-inflammatory conditions
WO2003020039A1 (en) 2001-08-28 2003-03-13 Rush-Presbyterian-St. Luke's Medical Center Immune tolerance to predetermined antigens
CA2468424A1 (en) 2001-11-27 2003-06-05 Cecilia Farre A method for combined parallel agent delivery and electroporation for cell structures and use thereof
US20030133922A1 (en) 2002-01-15 2003-07-17 Kasha John R. Oral tolerance using allogeneic platelets in ITP
US7501278B2 (en) 2002-06-05 2009-03-10 Panasonic Corporation Extracellular potential measuring device and method for fabricating the same
GB0214528D0 (en) 2002-06-24 2002-08-07 Univ Aberdeen Materials and methods for induction of immune tolerance
CA2513072A1 (en) 2003-01-09 2004-07-29 Invitrogen Corporation Cellular delivery and activation polypeptide-nucleic acid complexes
US20060134067A1 (en) 2003-02-18 2006-06-22 Maxcyte, Inc. Loading of cells with antigens by electroporation
WO2005123905A1 (ja) 2004-06-17 2005-12-29 Ken Nakata 生体力学的刺激負荷による細胞培養方法及びその装置
DE602005023616D1 (de) 2004-07-27 2010-10-28 Dsm Ip Assets Bv Verfahren zur herstellung von verbundfasern aus kohlenstoffnanoröhren und ultrahochmolekularem polyethylen
FR2873925B1 (fr) 2004-08-05 2006-10-13 Erytech Pharma Soc Par Actions Procede et dispositif de lyse-rescellement pour l'incorporation de principe actif notamment asparaginase ou inositol hexaphosphate, dans des erythrocytes
CN100591761C (zh) 2004-08-19 2010-02-24 加的夫大学学院咨询有限公司 呈递抗原的人γδT细胞的制备和在免疫治疗中的用途
US20060134772A1 (en) 2004-11-18 2006-06-22 The Regents Of The University Of California System for locating cells and for cellular analysis
US8420078B2 (en) 2005-03-10 2013-04-16 Yeda Research And Development Co. Ltd. Methods and immunogenic cell preparations for treating antigen-associated diseases
US7704743B2 (en) 2005-03-30 2010-04-27 Georgia Tech Research Corporation Electrosonic cell manipulation device and method of use thereof
KR100760309B1 (ko) 2005-07-06 2007-10-05 한국과학기술원 미소필터를 이용한 미소입자 변형성 분석기
US7993821B2 (en) 2005-08-11 2011-08-09 University Of Washington Methods and apparatus for the isolation and enrichment of circulating tumor cells
US9005974B2 (en) 2005-12-09 2015-04-14 Academish Medisch Centrum Bij De Universiteit Van Amsterdam Means and methods for influencing the stability of cells
WO2007097934A2 (en) 2006-02-17 2007-08-30 Elusys Therapeutics, Inc. Methods and compositions for using erythrocytes as carriers for delivery of drugs
US8293524B2 (en) 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
US20070249038A1 (en) 2006-04-21 2007-10-25 Andrea Adamo Microfluidic device for single cell targeted operations
CA2662826A1 (en) 2006-08-17 2008-02-21 Massachusetts Institute Of Technology Method and apparatus for microfluidic injection
WO2008098094A1 (en) 2007-02-06 2008-08-14 Network Biosystems, Inc. Devices and methods for the performance of miniaturized in vitro assays
US20080311140A1 (en) 2007-05-29 2008-12-18 Baylor College Of Medicine Antigen specific immunosuppression by dendritic cell therapy
US8841135B2 (en) * 2007-06-20 2014-09-23 University Of Washington Biochip for high-throughput screening of circulating tumor cells
US8420891B2 (en) 2007-06-21 2013-04-16 National University Corporation Nagoya University Method for introducing foreign substance into cell having cell wall
FR2919804B1 (fr) 2007-08-08 2010-08-27 Erytech Pharma Composition et vaccin therapeutique anti-tumoral
GB0718160D0 (en) 2007-09-18 2007-10-24 Medical Res Council Methods
EP2057998A1 (en) 2007-10-31 2009-05-13 Universitätsklinikum Hamburg-Eppendorf Use of modified cells for the treatment of multiple sclerosis
KR100891487B1 (ko) * 2007-11-01 2009-04-01 한국과학기술원 미세유체제어기술 기반으로 한 난모세포 또는 난자의 핵제거 시스템
CN101874201B (zh) 2007-11-28 2012-08-15 柯尼卡美能达精密光学株式会社 血液流动性测量装置及血液流动性测量方法
US20090280518A1 (en) 2008-05-12 2009-11-12 Massachusetts Institute Of Technology System for high throughput measurement of mechanical properties of cells
JP5137129B2 (ja) 2008-07-23 2013-02-06 国立大学法人山口大学 血液細胞の力学的特性測定装置
US9115340B2 (en) 2008-08-08 2015-08-25 Agency For Science Technology & Research Microfluidic continuous flow device
US8211656B2 (en) 2008-08-13 2012-07-03 The Invention Science Fund I, Llc Biological targeting compositions and methods of using the same
US20110091973A1 (en) 2008-12-09 2011-04-21 Glaser Larry F Modified and fusion enhanced erythrocytes, cells and uses thereof
US9157550B2 (en) 2009-01-05 2015-10-13 The Board Of Trustees Of The University Of Illinois Microfluidic systems and methods
WO2010105135A1 (en) 2009-03-13 2010-09-16 Tufts University Methods, apparatuses, and kits for introducing genetic material into living cells
US9017991B2 (en) 2009-03-13 2015-04-28 Tufts University Methods tip assemblies and kits for introducing material into cells
US20120107925A1 (en) 2009-03-20 2012-05-03 Mo Huang Li Devices for separating cells and methods of using them
JP4849278B2 (ja) * 2009-03-27 2012-01-11 セイコーエプソン株式会社 細胞処理デバイス、細胞処理カートリッジおよび体液処理システム
WO2010129671A2 (en) 2009-05-08 2010-11-11 Chauncey Sayre Method of and apparatus for changing one type of cell into another type of cell
GB0909754D0 (en) 2009-06-05 2009-07-22 Magnani Mauro Drug delivery systems
EP2449135B1 (en) 2009-06-30 2016-01-06 Sangamo BioSciences, Inc. Rapid screening of biologically active nucleases and isolation of nuclease-modified cells
CA2766907A1 (en) 2009-07-06 2011-01-13 Novartis Ag Self replicating rna molecules and uses thereof
KR101125060B1 (ko) * 2009-07-22 2012-03-21 한국과학기술원 입자를 포획하는 미세유체 소자 및 이를 이용한 입자 포획 방법
US9364831B2 (en) 2009-08-08 2016-06-14 The Regents Of The University Of California Pulsed laser triggered high speed microfluidic switch and applications in fluorescent activated cell sorting
AU2010311515B2 (en) 2009-10-27 2014-02-20 Erytech Pharma Composition to induce specific immune tolerance
CN102791865B (zh) 2009-12-21 2015-07-01 凯津公司 用于转染原生质体的改进技术
MX344460B (es) 2009-12-23 2016-12-14 Cytovera Inc * Sistema y método para la filtración de partículas.
US9005579B2 (en) 2010-01-05 2015-04-14 Contrafect Corporation Methods and compositions for enhanced immunological therapy and targeting of gram-positive bacteria
US8844570B2 (en) 2010-02-01 2014-09-30 California Institute Of Technology Generating binary states using a microfluidic channel
JP5704590B2 (ja) * 2010-02-05 2015-04-22 国立大学法人東京農工大学 サイズ選択マイクロキャビティアレイを用いた循環腫瘍細胞の検出
SG183468A1 (en) 2010-03-04 2012-09-27 Univ Singapore Microfluidics sorter for cell detection and isolation
US20110293558A1 (en) 2010-03-22 2011-12-01 Massachusetts Institute Of Technology Material properties of t cells and related methods and compositions
WO2012074588A2 (en) 2010-08-30 2012-06-07 President And Fellows Of Harvard College Shear controlled release for stenotic lesions and thrombolytic therapies
DE11787873T1 (de) 2010-11-25 2013-12-12 Imnate Sarl Immugene Peptide zur Verwendung bei der Vorbeugung und/oder Behandlung von Infektionskrankheiten, Autoimmunerkrankungen, Immunreaktionen auf Allofaktoren, allergischen Erkrankungen, Tumoren, Transplantatabstossungen und Immunreaktionen auf virale Vektoren
CA2825093A1 (en) * 2011-01-19 2012-07-26 The University Of British Columbia Apparatus and method for particle separation
MX363013B (es) 2011-02-25 2019-03-04 Recombinetics Inc Animales genéticamente modificados y métodos para su obtención.
DK2681306T3 (en) 2011-02-28 2019-04-23 Harvard College CELL CULTURE SYSTEM
US9902990B2 (en) 2011-05-27 2018-02-27 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
MX2013013243A (es) 2011-07-05 2014-05-30 Sotio As Medios y metodos para activar la inmunoterapia celular del cancer utilizando celulas cancerosas destruidas mediante presion hidrostatica y celulas dendriticas.
US20130065314A1 (en) 2011-08-25 2013-03-14 Phytocentric Biotech Inc. Algal transformation systems, compositions and methods
PT2768942T (pt) 2011-10-17 2020-01-21 Massachusetts Inst Technology Entrega intracelular
CN104684546A (zh) 2012-06-07 2015-06-03 哈佛大学校长及研究员协会 用于药物靶向的纳米疗法
US9255245B2 (en) 2012-07-03 2016-02-09 Agilent Technologies, Inc. Sample probes and methods for sampling intracellular material
AU2013335451C1 (en) 2012-10-23 2024-07-04 Toolgen Incorporated Composition for cleaving a target DNA comprising a guide RNA specific for the target DNA and Cas protein-encoding nucleic acid or Cas protein, and use thereof
CN102925337B (zh) 2012-11-08 2014-06-18 武汉友芝友生物制药有限公司 一种微流体细胞捕获芯片及其制备方法
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
GB201300052D0 (en) 2013-01-03 2013-02-20 Transimmune Ag Method for obtaining immuno-suppressive dendritic cells
GB201300049D0 (en) 2013-01-03 2013-02-20 Transimmune Ag Method for obtaining immuno-stimulatory dendritic cells
US20140295490A1 (en) 2013-01-30 2014-10-02 Asta Fluidic Technologies, Inc. Fetal red blood cell detection
US9725709B2 (en) 2013-03-12 2017-08-08 OpenCell Technologies, Inc. Intracellular delivery and transfection methods and devices
CN105452471A (zh) 2013-03-15 2016-03-30 加利福尼亚大学董事会 使用光热平台向活细胞中的高通量运输物递送
KR20140115560A (ko) 2013-03-21 2014-10-01 인하대학교 산학협력단 기계적 스트레스를 이용한 단세포 생물체 개질 방법 및 이에 사용되는 장치
WO2014165707A2 (en) 2013-04-03 2014-10-09 Memorial Sloan-Kettering Cancer Center Effective generation of tumor-targeted t-cells derived from pluripotent stem cells
KR101508974B1 (ko) * 2013-05-14 2015-04-07 한국과학기술연구원 종양 세포의 검출장치 및 검출방법
KR102243597B1 (ko) 2013-08-16 2021-04-26 메사추세츠 인스티튜트 오브 테크놀로지 세포로의 선택적 물질 전달
CA2927947A1 (en) 2013-10-22 2015-04-30 Cellanyx Diagnostics, Llc Systems, devices and methods for microfluidic culturing, manipulation and analysis of tissues and cells
US10137673B2 (en) 2013-12-31 2018-11-27 Canon U.S. Life Sciences, Inc. Methods and systems for continuous flow cell lysis in a microfluidic device
MA39819A (fr) 2014-04-01 2017-02-08 Rubius Therapeutics Inc Méthodes et compositions d'immunomodulation
WO2015161276A2 (en) 2014-04-18 2015-10-22 Editas Medicine, Inc. Crispr-cas-related methods, compositions and components for cancer immunotherapy
EP3164482B1 (en) 2014-07-03 2024-05-22 Massachusetts Institute of Technology Apparatus and method for optimization of cell electroporation
KR20170074235A (ko) 2014-10-31 2017-06-29 메사추세츠 인스티튜트 오브 테크놀로지 면역 세포로의 생체분자의 전달
CN107002089B (zh) 2014-11-14 2021-09-07 麻省理工学院 化合物和组合物向细胞中的破坏和场实现的递送
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
CA2973117C (en) 2015-01-07 2019-04-16 Indee. Inc. A method for mechanical and hydrodynamic microfluidic transfection and apparatus therefor
CN107250373A (zh) 2015-01-12 2017-10-13 麻省理工学院 通过微流体递送实现的基因编辑
US20180135012A1 (en) 2015-05-13 2018-05-17 Rubius Therapeutics, Inc. Membrane-receiver complex therapeutics
GB201513278D0 (en) 2015-07-03 2015-09-09 Transimmune Ag And Yale University Device and method for obtaining immunostimulatory antigen-presenting cells
AU2016289530B2 (en) 2015-07-09 2021-05-06 Massachusetts Institute Of Technology Delivery of materials to anucleate cells
EP3344575B1 (en) 2015-09-04 2020-04-15 SQZ Biotechnologies Company Intracellular delivery of biomolecules to cells comprising a cell wall
PL3344747T3 (pl) 2015-09-04 2023-06-19 Sqz Biotechnologies Company Wewnątrzkomórkowe dostarczanie biocząsteczek za pośrednictwem powierzchni z porami
CA3009369A1 (en) 2015-12-22 2017-06-29 Sangui Bio Pty. Ltd Therapeutic methods using erythrocytes
SI3402491T1 (sl) 2016-01-11 2022-05-31 Rubius Therapeutics, Inc. Sestavki in postopki v zvezi z multimodalnimi terapevtskimi celičnimi sistemi za indikacije raka
JP7033535B2 (ja) 2016-01-12 2022-03-10 スクイーズ バイオテクノロジーズ カンパニー 複合体の細胞内送達
CA3023092A1 (en) 2016-05-03 2017-11-09 Sqz Biotechnologies Company Intracellular delivery of biomolecules to induce tolerance
AU2017259988B2 (en) 2016-05-03 2023-04-27 Sqz Biotechnologies Company Intracellular delivery of biomolecules to induce tolerance
US20170326213A1 (en) 2016-05-16 2017-11-16 Augusta University Research Institute, Inc. Protein-Coupled Red Blood Cell Compositions and Methods of Their Use
CN106244543A (zh) 2016-07-29 2016-12-21 北京时合生物科技有限公司 一种pbmc体外诱导分化成为树突状细胞的方法
WO2018089497A1 (en) 2016-11-08 2018-05-17 Georgia Tech Research Corporation Methods for convectively-driven intracellular delivery

Also Published As

Publication number Publication date
US20190030536A1 (en) 2019-01-31
US11806714B2 (en) 2023-11-07
KR102304167B1 (ko) 2021-09-24
US10870112B2 (en) 2020-12-22
US20210170411A1 (en) 2021-06-10
MX2016002058A (es) 2016-08-17
SG10201801188XA (en) 2018-03-28
KR102386122B1 (ko) 2022-04-14
US10124336B2 (en) 2018-11-13
MX382654B (es) 2025-03-13
AU2014306423B2 (en) 2019-04-18
WO2015023982A1 (en) 2015-02-19
JP2016527907A (ja) 2016-09-15
KR20220049050A (ko) 2022-04-20
CN105848793B (zh) 2019-03-05
ES2865107T3 (es) 2021-10-15
IL244113A0 (en) 2016-04-21
EP3033184B1 (en) 2021-03-31
KR102530914B1 (ko) 2023-05-11
KR20210043736A (ko) 2021-04-21
EP3848695A1 (en) 2021-07-14
MX2021005777A (es) 2021-07-02
JP6502940B2 (ja) 2019-04-17
SG11201601927SA (en) 2016-04-28
US20160193605A1 (en) 2016-07-07
CA2921579C (en) 2021-09-21
EP3033184A4 (en) 2017-03-29
KR20160061332A (ko) 2016-05-31
CN105848793A (zh) 2016-08-10
AU2014306423A1 (en) 2016-03-17
KR102243597B1 (ko) 2021-04-26
KR20210116682A (ko) 2021-09-27
AU2019204480A1 (en) 2019-07-11
IL244113B (en) 2022-05-01
CA2921579A1 (en) 2015-02-19
EP3033184A1 (en) 2016-06-22

Similar Documents

Publication Publication Date Title
PL3033184T3 (pl) Selektywne dostarczanie materiału do komórek
IL245573B (en) Cell
DK3461491T3 (da) Forbedret adoptiv celleterapi
DK3812453T3 (da) Metabolisk optimeret cellkultur
LT3091141T (lt) Lengvesnės grindų plokštės iš mažiau medžiagų
EP2955772A4 (en) BATTERY PACK
ME03557B (me) Privremena zaštiтa normalnih ćelija током hemoterapije
ES1078818Y (es) Cápsula para preparar infusiones
FR3003076B1 (fr) Supercondensateur electrochimique
EP2911230A4 (en) CELL
EP2930778A4 (en) Electrode assembly
PL3030519T3 (pl) Trójpierścieniowe związki benzoksaborolu i ich zastosowania
EP3066193C0 (en) CELL CULTURE PROCESS
PL3007261T3 (pl) Nowy akumulator
EP2866277A4 (en) BATTERY PACK
EP3057155A4 (en) BATTERY PACK
PL2900676T3 (pl) Ulepszony sposób acylowania majtansynolu
PL3066228T3 (pl) Sposób otrzymywania materiału fotokatalitycznego
EP2982171A4 (en) BACKGROUND SEARCH OF SMALL CELLS
EP3067936A4 (en) SOLAR CELL
EP3062822C0 (en) CELL TRANSPORT
FI20136038L (fi) Uudet materiaalit
EP3067940A4 (en) SOLAR CELL
FR3015112B1 (fr) Cellule photovoltaique
TH1501000861A (th) วัสดุแอคทีฟอิเล็กโทรดลบ