PL413941A1 - Sposób syntezy i oczyszczania nukleozydu i/lub nukleotydu, zmodyfikowany nukleozyd i/lub nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleozyd i/lub nukleotyd oraz zastosowanie biblioteki oligonuklotydów - Google Patents

Sposób syntezy i oczyszczania nukleozydu i/lub nukleotydu, zmodyfikowany nukleozyd i/lub nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleozyd i/lub nukleotyd oraz zastosowanie biblioteki oligonuklotydów

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Publication number
PL413941A1
PL413941A1 PL413941A PL41394115A PL413941A1 PL 413941 A1 PL413941 A1 PL 413941A1 PL 413941 A PL413941 A PL 413941A PL 41394115 A PL41394115 A PL 41394115A PL 413941 A1 PL413941 A1 PL 413941A1
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PL
Poland
Prior art keywords
nucleotide
nucleoside
modified nucleoside
application
purification
Prior art date
Application number
PL413941A
Other languages
English (en)
Other versions
PL239946B1 (pl
Inventor
Przemysław Jurek
Filip Jeleń
Maciej Mazurek
Piotr Jakimowicz
Original Assignee
Pure Biologics Spółka Z Ograniczoną Odpowiedzialnością
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pure Biologics Spółka Z Ograniczoną Odpowiedzialnością filed Critical Pure Biologics Spółka Z Ograniczoną Odpowiedzialnością
Priority to PL413941A priority Critical patent/PL239946B1/pl
Priority to PT168469443T priority patent/PT3350195T/pt
Priority to ES16846944T priority patent/ES2885824T3/es
Priority to PCT/PL2016/050038 priority patent/WO2017048147A1/en
Priority to PL16846944T priority patent/PL3350195T3/pl
Priority to US15/760,915 priority patent/US10450673B2/en
Priority to DK16846944.3T priority patent/DK3350195T3/da
Priority to SI201631318T priority patent/SI3350195T1/sl
Priority to EP16846944.3A priority patent/EP3350195B9/en
Priority to HUE16846944A priority patent/HUE055954T2/hu
Priority to LTEPPCT/PL2016/050038T priority patent/LT3350195T/lt
Publication of PL413941A1 publication Critical patent/PL413941A1/pl
Publication of PL239946B1 publication Critical patent/PL239946B1/pl

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/073—Pyrimidine radicals with 2-deoxyribosyl as the saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób syntezy i oczyszczania nukleozydu i/lub nukleotydu, stanowiącego mono-, di- lub trójfosforan, polegający na tym, że przeprowadza się reakcje cykloaddycji alkinowo-azydkowej Huisgena związku o wzorze ogólnym 1 ze związkiem wybranym z grupy związków o wzorach ogólnych od 2 do 5. Przedmiotem zgłoszenia jest również zmodyfikowany nukleozyd i/lub nukleotyd o wzorze ogólnym 1, cząsteczka DNA i biblioteka oligonukleotydów zawierające jeden lub więcej zmodyfikowany nukleotyd oraz zastosowanie biblioteki oligonukleotydów do selekcji aptamerów.
PL413941A 2015-09-17 2015-09-17 Sposób syntezy i oczyszczania nukleotydu, zmodyfikowany nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleotyd oraz zastosowanie biblioteki oligonukleotydów PL239946B1 (pl)

Priority Applications (11)

Application Number Priority Date Filing Date Title
PL413941A PL239946B1 (pl) 2015-09-17 2015-09-17 Sposób syntezy i oczyszczania nukleotydu, zmodyfikowany nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleotyd oraz zastosowanie biblioteki oligonukleotydów
PT168469443T PT3350195T (pt) 2015-09-17 2016-09-13 Método de síntese e purificação de um nucleósido e/ou um nucleótido, um nucleósido e/ou nucleótido modificado, uma molécula de adn e uma biblioteca de oligonucleótidos compreendendo o referido nucleósido e/ou nucleótido modificado e a utilização da referida biblioteca de oligonucleótidos
ES16846944T ES2885824T3 (es) 2015-09-17 2016-09-13 Procedimiento de síntesis y purificación de un nucleósido y/o un nucleótido, un nucleósido y/o nucleótido modificados, una molécula de ADN y una biblioteca de oligonucleótidos que comprende dicho nucleósido y/o nucleótido modificados y la utilización de dicha biblioteca de oligonucleótidos
PCT/PL2016/050038 WO2017048147A1 (en) 2015-09-17 2016-09-13 The method of synthesis and purification of a nucleoside and/or a nucleotide, a modified nucleoside and/or nucleotide, a dna molecule and an oligonucleotide library comprising said modified nucleoside and/or nucleotide and the use of said oligonucleotide library
PL16846944T PL3350195T3 (pl) 2015-09-17 2016-09-13 Sposób syntezy i oczyszczania nukleozydu i/lub nukleotydu, zmodyfikowany nukleozyd i/lub nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleozyd i/lub nukleotyd oraz zastosowanie biblioteki oligonukleotydów
US15/760,915 US10450673B2 (en) 2015-09-17 2016-09-13 Method of synthesis and purification of a nucleoside and/or a nucleotide, a modified nucleoside and/or nucleotide, a DNA molecule and an oligonucleotide library comprising said modified nucleoside and/or nucleotide and the use of said oligonucleotide library
DK16846944.3T DK3350195T3 (da) 2015-09-17 2016-09-13 Fremgangsmåde til syntese og oprensning af nukleosid og/eller nukleotid, modificeret nukleosid og/eller nukleotid, dna-molekyle og oligonukleotidbibliotek omfattende det modificerede nukleosid og/eller nukleotid og anvendelse af oligonukleotidet
SI201631318T SI3350195T1 (sl) 2015-09-17 2016-09-13 Postopek sinteze in čiščenja nukleozida in/ali nukleotida, modificiranega nukleozida in/ali nukleotida, molekule DNA in knjižnice oligonukleotidov, ki vsebije navedeni modificirani nukleozid in/ali nukleotid, ter uporaba navedene knjižnice oligonukleotidov
EP16846944.3A EP3350195B9 (en) 2015-09-17 2016-09-13 The method of synthesis and purification of a nucleoside and/or a nucleotide, a modified nucleoside and/or nucleotide, a dna molecule and an oligonucleotide library comprising said modified nucleoside and/or nucleotide and the use of said oligonucleotide library
HUE16846944A HUE055954T2 (hu) 2015-09-17 2016-09-13 Eljárás nukleozid és/vagy nukleotid szintézisére és tisztítására, módosított nukleozid és/vagy nukleotid, DNS-molekula és oligonukleotidkönyvtár a módosított nukleoziddal és/vagy nukleotiddal, és az oligonukleotidkönyvtár alkalmazása
LTEPPCT/PL2016/050038T LT3350195T (lt) 2015-09-17 2016-09-13 Nukleozido ir/arba nukleotido sintezės ir gryninimo būdas, modifikuotas nukleozidas ir/arba nukleotidas, dnr molekulė ir oligonukleotidu biblioteka, apimanti šį modifikuotą nukleozidą ir/arba nukleotidą, ir šios oligonukleotidu bibliotekos panaudojimas

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL413941A PL239946B1 (pl) 2015-09-17 2015-09-17 Sposób syntezy i oczyszczania nukleotydu, zmodyfikowany nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleotyd oraz zastosowanie biblioteki oligonukleotydów

Publications (2)

Publication Number Publication Date
PL413941A1 true PL413941A1 (pl) 2017-03-27
PL239946B1 PL239946B1 (pl) 2022-01-31

Family

ID=58289513

Family Applications (2)

Application Number Title Priority Date Filing Date
PL413941A PL239946B1 (pl) 2015-09-17 2015-09-17 Sposób syntezy i oczyszczania nukleotydu, zmodyfikowany nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleotyd oraz zastosowanie biblioteki oligonukleotydów
PL16846944T PL3350195T3 (pl) 2015-09-17 2016-09-13 Sposób syntezy i oczyszczania nukleozydu i/lub nukleotydu, zmodyfikowany nukleozyd i/lub nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleozyd i/lub nukleotyd oraz zastosowanie biblioteki oligonukleotydów

Family Applications After (1)

Application Number Title Priority Date Filing Date
PL16846944T PL3350195T3 (pl) 2015-09-17 2016-09-13 Sposób syntezy i oczyszczania nukleozydu i/lub nukleotydu, zmodyfikowany nukleozyd i/lub nukleotyd, cząsteczka DNA i biblioteka oligonukleotydów zawierające zmodyfikowany nukleozyd i/lub nukleotyd oraz zastosowanie biblioteki oligonukleotydów

Country Status (10)

Country Link
US (1) US10450673B2 (pl)
EP (1) EP3350195B9 (pl)
DK (1) DK3350195T3 (pl)
ES (1) ES2885824T3 (pl)
HU (1) HUE055954T2 (pl)
LT (1) LT3350195T (pl)
PL (2) PL239946B1 (pl)
PT (1) PT3350195T (pl)
SI (1) SI3350195T1 (pl)
WO (1) WO2017048147A1 (pl)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL442186A1 (pl) * 2022-09-02 2024-03-04 Pure Biologics Spółka Akcyjna Sposób syntezy i oczyszczania nukleotydu i/lub nukleozydu, zmodyfikowany nukleotyd i/lub nukleozyd, cząsteczka DNA zawierająca jedno- lub dwuniciowy łańcuch oligonukleotydów, biblioteka oligonukleotydów, zastosowanie biblioteki oligonukleotydów
US20260000702A1 (en) 2024-06-26 2026-01-01 Flagship Pioneering Innovations Vii, Llc Therapeutic circular dna forms

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6175001B1 (en) 1998-10-16 2001-01-16 The Scripps Research Institute Functionalized pyrimidine nucleosides and nucleotides and DNA's incorporating same
EP2176277B1 (en) 2007-07-19 2016-02-24 Georgia State University Research Foundation, Inc. Nucleotides and aptamers containing boronic acid groups having biased binding to glycosylated proteins, and uses thereof

Also Published As

Publication number Publication date
PL239946B1 (pl) 2022-01-31
HUE055954T2 (hu) 2022-01-28
DK3350195T3 (da) 2021-08-30
EP3350195A4 (en) 2019-07-03
EP3350195A1 (en) 2018-07-25
US10450673B2 (en) 2019-10-22
US20180265862A1 (en) 2018-09-20
WO2017048147A1 (en) 2017-03-23
ES2885824T3 (es) 2021-12-15
EP3350195B1 (en) 2021-06-02
SI3350195T1 (sl) 2021-11-30
EP3350195B9 (en) 2021-09-29
PL3350195T3 (pl) 2021-10-11
LT3350195T (lt) 2021-09-10
PT3350195T (pt) 2021-09-06

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