PL437111A1 - Oligonucleotide primers for detecting Phytophthora spp. phytopathogenic microorganisms and a method for their detection - Google Patents

Oligonucleotide primers for detecting Phytophthora spp. phytopathogenic microorganisms and a method for their detection

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Publication number
PL437111A1
PL437111A1 PL437111A PL43711121A PL437111A1 PL 437111 A1 PL437111 A1 PL 437111A1 PL 437111 A PL437111 A PL 437111A PL 43711121 A PL43711121 A PL 43711121A PL 437111 A1 PL437111 A1 PL 437111A1
Authority
PL
Poland
Prior art keywords
detection
oligonucleotide primers
phytopathogenic microorganisms
phytophthora spp
detecting
Prior art date
Application number
PL437111A
Other languages
Polish (pl)
Other versions
PL242988B1 (en
Inventor
Jacek Panek
Dominika Malarczyk
Magdalena Frąc
Original Assignee
Instytut Agrofizyki Im. Bohdana Dobrzańskiego Polskiej Akademii Nauk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Instytut Agrofizyki Im. Bohdana Dobrzańskiego Polskiej Akademii Nauk filed Critical Instytut Agrofizyki Im. Bohdana Dobrzańskiego Polskiej Akademii Nauk
Priority to PL437111A priority Critical patent/PL242988B1/en
Publication of PL437111A1 publication Critical patent/PL437111A1/en
Publication of PL242988B1 publication Critical patent/PL242988B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6893—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for protozoa
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia są startery oligonukleotydowe do wykrywania patogenów należących do rodzaju Phytophthora o sekwencjach nr l, 2, 3, 4, 5 i 6 przedstawionych na liście sekwencji. Zgłoszenie obejmuje też sposób wykrywania fitopatogenicznego organizmu z rodzaju Phytophthora, w którym w reakcji LAMP z zastosowaniem zestawu starterów dochodzi do amplifikacji określonego fragmentu DNA, objawiającej się pojawieniem się krzywej amplifikacji, potwierdzając detekcję powstałego produktu, charakteryzujący się tym że, zestaw starterów stanowią startery oligonukleotydowe przedstawione na liście sekwencji.The subject of the application are oligonucleotide primers for the detection of pathogens belonging to the genus Phytophthora with the sequences No. 1, 2, 3, 4, 5 and 6 shown in the sequence listing. The application also covers a method of detecting a phytophthora organism of the genus Phytophthora, in which, in the LAMP reaction with the use of a set of primers, a specific DNA fragment is amplified, which is manifested by the appearance of an amplification curve, confirming the detection of the resulting product, characterized in that the primer set consists of the oligonucleotide primers shown in the sequence list.

PL437111A 2021-02-24 2021-02-24 Oligonucleotide primers for detecting Phytophthora spp. phytopathogenic microorganisms and a method for their detection PL242988B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL437111A PL242988B1 (en) 2021-02-24 2021-02-24 Oligonucleotide primers for detecting Phytophthora spp. phytopathogenic microorganisms and a method for their detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL437111A PL242988B1 (en) 2021-02-24 2021-02-24 Oligonucleotide primers for detecting Phytophthora spp. phytopathogenic microorganisms and a method for their detection

Publications (2)

Publication Number Publication Date
PL437111A1 true PL437111A1 (en) 2022-08-29
PL242988B1 PL242988B1 (en) 2023-05-29

Family

ID=83723955

Family Applications (1)

Application Number Title Priority Date Filing Date
PL437111A PL242988B1 (en) 2021-02-24 2021-02-24 Oligonucleotide primers for detecting Phytophthora spp. phytopathogenic microorganisms and a method for their detection

Country Status (1)

Country Link
PL (1) PL242988B1 (en)

Also Published As

Publication number Publication date
PL242988B1 (en) 2023-05-29

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