PL439738A1 - Method for producing 4',5-dihydroxy-7-O-β-D-(4''-O-methylglucopyranosyl)-isoflavone - Google Patents

Method for producing 4',5-dihydroxy-7-O-β-D-(4''-O-methylglucopyranosyl)-isoflavone

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Publication number
PL439738A1
PL439738A1 PL439738A PL43973821A PL439738A1 PL 439738 A1 PL439738 A1 PL 439738A1 PL 439738 A PL439738 A PL 439738A PL 43973821 A PL43973821 A PL 43973821A PL 439738 A1 PL439738 A1 PL 439738A1
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PL
Poland
Prior art keywords
methylglucopyranosyl
isoflavone
dihydroxy
formula
hours
Prior art date
Application number
PL439738A
Other languages
Polish (pl)
Other versions
PL247265B1 (en
Inventor
Monika Dymarska
Edyta Kostrzewa-Susłow
Tomasz Janeczko
Original Assignee
Uniwersytet Przyrodniczy we Wrocławiu
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Publication date
Application filed by Uniwersytet Przyrodniczy we Wrocławiu filed Critical Uniwersytet Przyrodniczy we Wrocławiu
Priority to PL439738A priority Critical patent/PL247265B1/en
Publication of PL439738A1 publication Critical patent/PL439738A1/en
Publication of PL247265B1 publication Critical patent/PL247265B1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
    • C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
    • C07H17/06—Benzopyran radicals
    • C07H17/065—Benzo[b]pyrans
    • C07H17/07—Benzo[b]pyran-4-ones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób wytwarzania 4',5-dihydroksy-7-O-β-D-(4"-O-metyloglukopiranozylo)-izoflawonu o wzorze 2, charakteryzujący się tym, że do podłoża odpowiedniego dla grzybów strzępkowych wprowadza się szczep Isaria fumosorosea KCH J2, następnie po upływie co najmniej 72 godzin do hodowli wprowadza się substrat, którym jest 4',5,7-trihydroksyizoflawon o wzorze 1, rozpuszczony w rozpuszczalniku organicznym mieszającym się z wodą, transformację prowadzi się w temperaturze od 20 do 30 stopni Celsjusza, przy ciągłym wstrząsaniu, co najmniej 96 godzin, po czym produkt ekstrahuje się rozpuszczalnikiem organicznym niemieszającym się z wodą i oczyszcza chromatograficznie, przy czym 4',5-dihydroksy-7-O-β-D-(4"-O-metyloglukopiranozylo)-izoflawon o wzorze 2 znajduje się we frakcji o najwyższej polarności.The subject of the application is a method for the production of 4',5-dihydroxy-7-O-β-D-(4"-O-methylglucopyranosyl)-isoflavone of the formula 2, characterized in that the strain Isaria fumosorosea is introduced into a substrate suitable for filamentous fungi KCH J2, then, after at least 72 hours, a substrate is introduced into the culture, which is 4',5,7-trihydroxyisoflavone of the formula 1, dissolved in an organic solvent miscible with water, the transformation is carried out at a temperature of 20 to 30 degrees Celsius with continuous shaking for at least 96 hours, after which the product is extracted with a water-immiscible organic solvent and purified by chromatography, the 4',5-dihydroxy-7-O-β-D-(4"-O-methylglucopyranosyl) -isoflavone of formula 2 is found in the fraction with the highest polarity.

PL439738A 2021-12-03 2021-12-03 Method for producing 4',5-dihydroxy-7-O-β-D-(4''-O-methylglucopyranosyl)-isoflavone PL247265B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL439738A PL247265B1 (en) 2021-12-03 2021-12-03 Method for producing 4',5-dihydroxy-7-O-β-D-(4''-O-methylglucopyranosyl)-isoflavone

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL439738A PL247265B1 (en) 2021-12-03 2021-12-03 Method for producing 4',5-dihydroxy-7-O-β-D-(4''-O-methylglucopyranosyl)-isoflavone

Publications (2)

Publication Number Publication Date
PL439738A1 true PL439738A1 (en) 2023-06-05
PL247265B1 PL247265B1 (en) 2025-06-02

Family

ID=86701152

Family Applications (1)

Application Number Title Priority Date Filing Date
PL439738A PL247265B1 (en) 2021-12-03 2021-12-03 Method for producing 4',5-dihydroxy-7-O-β-D-(4''-O-methylglucopyranosyl)-isoflavone

Country Status (1)

Country Link
PL (1) PL247265B1 (en)

Also Published As

Publication number Publication date
PL247265B1 (en) 2025-06-02

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