PL450095A1 - Sposób immobilizacji lipazy na powierzchni membrany polimerowej pokrytej warstwą aluminium - Google Patents

Sposób immobilizacji lipazy na powierzchni membrany polimerowej pokrytej warstwą aluminium

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Publication number
PL450095A1
PL450095A1 PL450095A PL45009524A PL450095A1 PL 450095 A1 PL450095 A1 PL 450095A1 PL 450095 A PL450095 A PL 450095A PL 45009524 A PL45009524 A PL 45009524A PL 450095 A1 PL450095 A1 PL 450095A1
Authority
PL
Poland
Prior art keywords
polymer membrane
aluminum layer
membrane covered
lipase
immobilizing lipase
Prior art date
Application number
PL450095A
Other languages
English (en)
Inventor
Agata Zdarta
Aleksandra Rybak
Ewa Kaczorek
Original Assignee
Politechnika Poznańska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Poznańska filed Critical Politechnika Poznańska
Priority to PL450095A priority Critical patent/PL450095A1/pl
Publication of PL450095A1 publication Critical patent/PL450095A1/pl

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób immobilizacja lipazy na powierzchni membrany polimerowej polegający na tym, że w reaktorze membranowym instaluje się membranę polimerową pokrytą aluminium o powierzchni 4,9 cm2, następnie dodaje się 10 mL roztworu lipazy Candida rugosa w buforze fosforanowym o pH 7 i stężeniu w zakresie od 1 do 10 mg/mL, korzystnie 5 mg/mL i prowadzi się proces immobilizacji w czasie 24 godzin przy ciągłym mieszaniu z prędkością 200 rpm.
PL450095A 2024-10-22 2024-10-22 Sposób immobilizacji lipazy na powierzchni membrany polimerowej pokrytej warstwą aluminium PL450095A1 (pl)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL450095A PL450095A1 (pl) 2024-10-22 2024-10-22 Sposób immobilizacji lipazy na powierzchni membrany polimerowej pokrytej warstwą aluminium

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL450095A PL450095A1 (pl) 2024-10-22 2024-10-22 Sposób immobilizacji lipazy na powierzchni membrany polimerowej pokrytej warstwą aluminium

Publications (1)

Publication Number Publication Date
PL450095A1 true PL450095A1 (pl) 2026-04-27

Family

ID=99547496

Family Applications (1)

Application Number Title Priority Date Filing Date
PL450095A PL450095A1 (pl) 2024-10-22 2024-10-22 Sposób immobilizacji lipazy na powierzchni membrany polimerowej pokrytej warstwą aluminium

Country Status (1)

Country Link
PL (1) PL450095A1 (pl)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ514271A (en) * 1999-03-22 2003-08-29 Enzymotec Ltd Surfactant-lipase complex immobilized on insoluble matrix

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ514271A (en) * 1999-03-22 2003-08-29 Enzymotec Ltd Surfactant-lipase complex immobilized on insoluble matrix

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
E. LASOŃ I IN.,: "CHEMIK, 2010, nr 2, tom 64, 15-17", LIPASE – CHARACTERIZATION, APPLICATIONS AND METHODS OF IMMOBILIZATION *
JÉSSICA MULINARI I IN.,: "Separation and Purification Technology, Volume 330, Part B, 01.02.2024, 125493, ISSN 1383-5866", IN-SITU IMMOBILIZATION OF LIPASE ON Α-ALUMINA MEMBRANE FOR OIL FOULING CONTROL AND CLEANING, DOI: https://doi.org/10.1016/j.seppur.2023.125493 *
SILVA, M.V.C., I IN.,: "World J Microbiol Biotechnol 34, 169, 2018", OPTIMIZATION OF THE PARAMETERS THAT AFFECT THE SYNTHESIS OF MAGNETIC COPOLYMER STYRENE-DIVINILBEZENE TO BE USED AS EFFICIENT MATRIX FOR IMMOBILIZING LIPASES., DOI: https://doi.org/10.1007/s11274-018-2553-1. *
ZORICA KNEZEVIC I IN.,: "Biochemical Engineering Journal, Volume 30, Issue 3, 2006, Pages 269-278, ISSN 1369-703X", IMMOBILIZATION OF LIPASE FROM CANDIDA RUGOSA ON EUPERGIT® C SUPPORTS BY COVALENT ATTACHMENT, DOI: https://doi.org/10.1016/j.bej.2006.05.009 *

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