PL450099A1 - Sposób immobilizacji proteazy na powierzchni membrany polimerowej - Google Patents

Sposób immobilizacji proteazy na powierzchni membrany polimerowej

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Publication number
PL450099A1
PL450099A1 PL450099A PL45009924A PL450099A1 PL 450099 A1 PL450099 A1 PL 450099A1 PL 450099 A PL450099 A PL 450099A PL 45009924 A PL45009924 A PL 45009924A PL 450099 A1 PL450099 A1 PL 450099A1
Authority
PL
Poland
Prior art keywords
polymer membrane
protease
immobilizing
immobilizing protease
membrane
Prior art date
Application number
PL450099A
Other languages
English (en)
Inventor
Agata Zdarta
Jakub Zdarta
Teofil Jesionowski
Agnieszka Rybarczyk
Original Assignee
Politechnika Poznańska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Poznańska filed Critical Politechnika Poznańska
Priority to PL450099A priority Critical patent/PL450099A1/pl
Publication of PL450099A1 publication Critical patent/PL450099A1/pl

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób immobilizacja proteazy na powierzchni membrany polimerowej polegający na tym, że w reaktorze membranowym instaluje się membranę polimerową pokrytą złotem o powierzchni 4.9 cm2, następnie dodaje się 10 mL roztworu proteazy Aspergillus saitoi w buforze tris-HCl o pH 7,5 i stężeniu w zakresie od 1 do 10 mg/mL, korzystnie 5 mg/mL i prowadzi się proces immobilizacji w czasie 24 godzin przy ciągłym mieszaniu z prędkością 200 rpm.
PL450099A 2024-10-22 2024-10-22 Sposób immobilizacji proteazy na powierzchni membrany polimerowej PL450099A1 (pl)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL450099A PL450099A1 (pl) 2024-10-22 2024-10-22 Sposób immobilizacji proteazy na powierzchni membrany polimerowej

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL450099A PL450099A1 (pl) 2024-10-22 2024-10-22 Sposób immobilizacji proteazy na powierzchni membrany polimerowej

Publications (1)

Publication Number Publication Date
PL450099A1 true PL450099A1 (pl) 2026-04-27

Family

ID=99547493

Family Applications (1)

Application Number Title Priority Date Filing Date
PL450099A PL450099A1 (pl) 2024-10-22 2024-10-22 Sposób immobilizacji proteazy na powierzchni membrany polimerowej

Country Status (1)

Country Link
PL (1) PL450099A1 (pl)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DUAN, C., MEYERHOFF, M.E.: "Mikrochim Acta 117, 195–206 (1995).", IMMOBILIZATION OF PROTEINS ON GOLD COATED POROUS MEMBRANES VIA AN ACTIVATED SELF-ASSEMBLED MONOLAYER OF THIOCTIC ACID, DOI: https://doi.org/10.1007/BF01244890 *
GARIMA GOYAL I IN.: "Sensors and Actuators B: Chemical, Volume 305, 2020, 127442,", PROTEASE FUNCTIONAL ASSAY ON MEMBRANE, DOI: https://doi.org/10.1016/j.snb.2019.127442. *
STEEGMÜLLER T.,: "Membranes, 24.01.2024, 14, 31.", DEVELOPMENT OF A NEW AFFINITY GOLD POLYMER MEMBRANE WITH IMMOBILIZED PROTEIN A, DOI: https:// doi.org/10.3390/membranes14020031. *
SUMANT PHADTARE I IN.: "Biotechnology and Bioengineering, Vol. 85, NO. 6, 2004,", IMMOBILIZATION AND BIOCATALYTIC ACTIVITY OF FUNGAL PROTEASE ON GOLD NANOPARTICLE LOADED ZEOLITE MICROSPHERES, DOI: 10.1002/bit.10856 *

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