PL450098A1 - Sposób immobilizacji lipazy na powierzchni membrany polimerowej - Google Patents

Sposób immobilizacji lipazy na powierzchni membrany polimerowej

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Publication number
PL450098A1
PL450098A1 PL450098A PL45009824A PL450098A1 PL 450098 A1 PL450098 A1 PL 450098A1 PL 450098 A PL450098 A PL 450098A PL 45009824 A PL45009824 A PL 45009824A PL 450098 A1 PL450098 A1 PL 450098A1
Authority
PL
Poland
Prior art keywords
polymer membrane
lipase
immobilizing lipase
immobilizing
membrane
Prior art date
Application number
PL450098A
Other languages
English (en)
Inventor
Agata Zdarta
Jakub Zdarta
Teofil Jesionowski
Agnieszka Rybarczyk
Original Assignee
Politechnika Poznańska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Poznańska filed Critical Politechnika Poznańska
Priority to PL450098A priority Critical patent/PL450098A1/pl
Publication of PL450098A1 publication Critical patent/PL450098A1/pl

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób immobilizacja lipazy na powierzchni membrany polimerowej polegający na tym, że w reaktorze membranowym instaluje się membranę polimerową pokrytą złotem o powierzchni 4,9 cm2, następnie dodaje się 10 roztworu lipazy Candida rugosa w buforze fosforanowym o pH 7 i stężeniu w zakresie od 1 do 10 mg/mL, korzystnie 5 mg/mL prowadzi się proces immobilizacji w czasie 24 godzin przy ciągłym mieszaniu z prędkością 200 rpm.
PL450098A 2024-10-22 2024-10-22 Sposób immobilizacji lipazy na powierzchni membrany polimerowej PL450098A1 (pl)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL450098A PL450098A1 (pl) 2024-10-22 2024-10-22 Sposób immobilizacji lipazy na powierzchni membrany polimerowej

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL450098A PL450098A1 (pl) 2024-10-22 2024-10-22 Sposób immobilizacji lipazy na powierzchni membrany polimerowej

Publications (1)

Publication Number Publication Date
PL450098A1 true PL450098A1 (pl) 2026-04-27

Family

ID=99547486

Family Applications (1)

Application Number Title Priority Date Filing Date
PL450098A PL450098A1 (pl) 2024-10-22 2024-10-22 Sposób immobilizacji lipazy na powierzchni membrany polimerowej

Country Status (1)

Country Link
PL (1) PL450098A1 (pl)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ELAHE DARVISHI I IN.,: "J Appl Biotechnol Rep., 20.09.2024;11(3): 1396-1405,", BIOCHEMICAL CHARACTERIZATION OF CANDIDA RUGOSA LIPASE IMMOBILIZED ON GREEN SYNTHESIZED GOLD NANOPARTICLES, DOI: doi 10.30491/JABR.2024.403588.1647 *
IOLE VENDITTI I IN.,: "Colloids and Surfaces B: Biointerfaces, Volume 131, 2015, Pages 93-101", CANDIDA RUGOSA LIPASE IMMOBILIZATION ON HYDROPHILIC CHARGED GOLD NANOPARTICLES AS PROMISING BIOCATALYSTS: ACTIVITY AND STABILITY INVESTIGATIONS, DOI: https://doi.org/10.1016/j.colsurfb.2015.04.046. *
XIAOYU DU I IN.,: "Nanoscale Research Letters, 2013, 8:180 str.: 2-6", EFFICIENT BIOCATALYST BY ENCAPSULATING LIPASE INTO NANOPOROUS GOLD, DOI: http://www.nanoscalereslett.com/content/8/1/180 *
ZORICA KNEZEVIC I IN.,: "Biochemical Engineering Journal, Volume 30, Issue 3, 2006, Pages 269-278", IMMOBILIZATION OF LIPASE FROM CANDIDA RUGOSA ON EUPERGIT® C SUPPORTS BY COVALENT ATTACHMENT, DOI: https://doi.org/10.1016/j.bej.2006.05.009 *

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