PL450106A1 - Method of immobilizing laccase on the surface of a membrane covered with an aluminum layer - Google Patents

Method of immobilizing laccase on the surface of a membrane covered with an aluminum layer

Info

Publication number
PL450106A1
PL450106A1 PL450106A PL45010624A PL450106A1 PL 450106 A1 PL450106 A1 PL 450106A1 PL 450106 A PL450106 A PL 450106A PL 45010624 A PL45010624 A PL 45010624A PL 450106 A1 PL450106 A1 PL 450106A1
Authority
PL
Poland
Prior art keywords
laccase
aluminum layer
immobilizing
membrane covered
immobilizing laccase
Prior art date
Application number
PL450106A
Other languages
Polish (pl)
Inventor
Agata Zdarta
Oliwia Rożnowska
Original Assignee
Politechnika Poznańska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Poznańska filed Critical Politechnika Poznańska
Priority to PL450106A priority Critical patent/PL450106A1/en
Publication of PL450106A1 publication Critical patent/PL450106A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób immobilizacja lakazy na powierzchni membrany polimerowej polegający na tym, że w reaktorze membranowym instaluje się membranę polimerową pokrytą aluminium o powierzchni 4,9 cm2, następnie dodaje się 10 mL roztworu lakazy Trametes versicolor w buforze octanowym o pH 5 i stężeniu w zakresie od 1 do 10 mg/mL, korzystnie 5 mg/mL i prowadzi się proces immobilizacji w czasie 24 godzin przy ciągłym mieszaniu z prędkością 200 rpm.The subject of the application is a method for immobilizing laccase on the surface of a polymer membrane, which consists in installing a polymer membrane coated with aluminum with a surface of 4.9 cm2 in a membrane reactor, then adding 10 mL of a solution of Trametes versicolor laccase in an acetate buffer with a pH of 5 and a concentration in the range of 1 to 10 mg/mL, preferably 5 mg/mL, and carrying out the immobilization process for 24 hours with continuous stirring at a speed of 200 rpm.

PL450106A 2024-10-22 2024-10-22 Method of immobilizing laccase on the surface of a membrane covered with an aluminum layer PL450106A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL450106A PL450106A1 (en) 2024-10-22 2024-10-22 Method of immobilizing laccase on the surface of a membrane covered with an aluminum layer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL450106A PL450106A1 (en) 2024-10-22 2024-10-22 Method of immobilizing laccase on the surface of a membrane covered with an aluminum layer

Publications (1)

Publication Number Publication Date
PL450106A1 true PL450106A1 (en) 2026-04-27

Family

ID=99547484

Family Applications (1)

Application Number Title Priority Date Filing Date
PL450106A PL450106A1 (en) 2024-10-22 2024-10-22 Method of immobilizing laccase on the surface of a membrane covered with an aluminum layer

Country Status (1)

Country Link
PL (1) PL450106A1 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010097619A1 (en) * 2009-02-25 2010-09-02 The University Of Hull Immobilized enzymes and co-factors

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010097619A1 (en) * 2009-02-25 2010-09-02 The University Of Hull Immobilized enzymes and co-factors

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ADAMIAN Y. I IN.,: "Front. Bioeng. Biotechnol., 2021, 9: 778239", RECENT DEVELOPMENTS IN THE IMMOBILIZATION OF LACCASE ON CARBONACEOUS SUPPORTS FOR ENVIRONMENTAL APPLICATIONS – A CRITICAL REVIEW, DOI: 10.3389/fbioe.2021.778239 *
ANJUM HAMID RATHER I IN.,: "Biotechnol. Bioeng., 2022, 119: 9-33", OVERVIEW IN IMMOBILIZATION OF ENZYMES ON SYNTHETIC POLYMERIC NANOFIBERS FABRICATED BY ELECTROSPINNING, DOI: 10.1002/bit.27963 *
CLAUDE JOLIVALT I IN.,: "Journal of Membrane Science, 2000, 180 (1), pp.103-113", IMMOBILIZATION OF LACCASE FROM TRAMETES VERSICOLOR ON A MODIFIED PVDF MICROFILTRATION MEMBRANE: CHARACTERIZATION OF THE GRAFTED SUPPORT AND APPLICATION IN REMOVING A PHENYLUREA PESTICIDE IN WASTEWATER, DOI: ff10.1016/S0376-7388(00)00522-6ff. ffhal-02698967f *
JAKUB ZDARTA I IN.,: "Chemosphere, 2022, 304, 135374", LACCASE IMMOBILIZATION IN POLYELECTROLYTE MULTILAYER MEMBRANES FOR 17Α-ETHYNYLESTRADIOL REMOVAL: BIOCATALYTIC APPROACH FOR PHARMACEUTICALS DEGRADATION, DOI: doi.org/10.1016/j.chemosphere *

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