PL450108A1 - Method of immobilizing protease on the surface of a polymer membrane - Google Patents

Method of immobilizing protease on the surface of a polymer membrane

Info

Publication number
PL450108A1
PL450108A1 PL450108A PL45010824A PL450108A1 PL 450108 A1 PL450108 A1 PL 450108A1 PL 450108 A PL450108 A PL 450108A PL 45010824 A PL45010824 A PL 45010824A PL 450108 A1 PL450108 A1 PL 450108A1
Authority
PL
Poland
Prior art keywords
polymer membrane
protease
immobilizing
immobilizing protease
membrane
Prior art date
Application number
PL450108A
Other languages
Polish (pl)
Inventor
Agata Zdarta
Oliwia Rożnowska
Original Assignee
Politechnika Poznańska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Poznańska filed Critical Politechnika Poznańska
Priority to PL450108A priority Critical patent/PL450108A1/en
Publication of PL450108A1 publication Critical patent/PL450108A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób immobilizacji proteazy na powierzchni membrany polimerowej polegającym na tym, że w reaktorze membranowym instaluje się membranę polimerową pokrytą aluminium o powierzchni 4,9 cm2, następnie dodaje się 10 mL roztworu proteazy Aspergillus saitoi w buforze tris-HCl o pH 7,5 i stężeniu w zakresie od 1 do 10 mg/mL, korzystnie 5 mg/mL i prowadzi się proces immobilizacji w czasie 24 godzin przy ciągłym mieszaniu z prędkością 200 rpm.The subject of the application is a method of immobilizing protease on the surface of a polymer membrane, which consists in installing a polymer membrane coated with aluminum with a surface of 4.9 cm2 in a membrane reactor, then adding 10 mL of Aspergillus saitoi protease solution in a tris-HCl buffer with a pH of 7.5 and a concentration in the range of 1 to 10 mg/mL, preferably 5 mg/mL, and carrying out the immobilization process for 24 hours with continuous stirring at a speed of 200 rpm.

PL450108A 2024-10-22 2024-10-22 Method of immobilizing protease on the surface of a polymer membrane PL450108A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL450108A PL450108A1 (en) 2024-10-22 2024-10-22 Method of immobilizing protease on the surface of a polymer membrane

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL450108A PL450108A1 (en) 2024-10-22 2024-10-22 Method of immobilizing protease on the surface of a polymer membrane

Publications (1)

Publication Number Publication Date
PL450108A1 true PL450108A1 (en) 2026-04-27

Family

ID=99547490

Family Applications (1)

Application Number Title Priority Date Filing Date
PL450108A PL450108A1 (en) 2024-10-22 2024-10-22 Method of immobilizing protease on the surface of a polymer membrane

Country Status (1)

Country Link
PL (1) PL450108A1 (en)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ANJUM HAMID RATHER I IN.,: "Biotechnol. Bioeng., 2022, 119: 9-33,", OVERVIEW IN IMMOBILIZATION OF ENZYMES ON SYNTHETIC POLYMERIC NANOFIBERS FABRICATED BY ELECTROSPINNING, DOI: 10.1002/bit.27963 *
FRANCESCO PETROSINO I IN.,: "Computation 2019, 7, 56", ENZYME IMMOBILIZATION ON POLYMER MEMBRANES: A QUANTUM AND MOLECULAR MECHANICS STUDY, DOI: 10.3390/computation7040056 *
GIVANILDO BEZERRA OLIVEIRA I IN.,: "Reactive and Functional Polymers, Volume 68, Issue 1, 2008, Pages 27-32,", ENZYME IMMOBILIZATION ON ANODIC ALUMINUM OXIDE/POLYETHYLENEIMINE OR POLYANILINE COMPOSITES,, DOI: https://doi.org/10.1016/j.reactfunctpolym.2007.10.009. *
LYU X. I IN.,: "Catalysts 2021, 11, 1211.", IMMOBILIZATION OF ENZYMES BY POLYMERIC MATERIALS, DOI: https://doi.org/10.3390/catal11101211 *

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