PL450096A1 - Method of immobilizing laccase on the surface of a membrane covered with a gold layer - Google Patents

Method of immobilizing laccase on the surface of a membrane covered with a gold layer

Info

Publication number
PL450096A1
PL450096A1 PL450096A PL45009624A PL450096A1 PL 450096 A1 PL450096 A1 PL 450096A1 PL 450096 A PL450096 A PL 450096A PL 45009624 A PL45009624 A PL 45009624A PL 450096 A1 PL450096 A1 PL 450096A1
Authority
PL
Poland
Prior art keywords
laccase
gold layer
immobilizing
membrane covered
membrane
Prior art date
Application number
PL450096A
Other languages
Polish (pl)
Inventor
Agata Zdarta
Jakub Zdarta
Teofil Jesionowski
Agnieszka Rybarczyk
Original Assignee
Politechnika Poznańska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Poznańska filed Critical Politechnika Poznańska
Priority to PL450096A priority Critical patent/PL450096A1/en
Publication of PL450096A1 publication Critical patent/PL450096A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób immobilizacja lakazy na powierzchni membrany polimerowej polegający na tym, że w reaktorze membranowym instaluje się membranę polimerową pokrytą złotem o powierzchni 4,9 cm2, następnie dodaje się 10 mL, roztworu lakazy Trametes versicolor w buforze fosforanowym o pH 5 i stężeniu w zakresie od 1 do 10 mg/mL, korzystnie 5 mg/mL i prowadzi się proces immobilizacji w czasie 24 godzin przy ciągłym mieszaniu z prędkością 200 rpm.The subject of the application is a method for immobilizing laccase on the surface of a polymer membrane, which consists in installing a gold-coated polymer membrane with a surface of 4.9 cm2 in a membrane reactor, then adding 10 mL of a solution of Trametes versicolor laccase in a phosphate buffer with a pH of 5 and a concentration in the range of 1 to 10 mg/mL, preferably 5 mg/mL, and carrying out the immobilization process for 24 hours with continuous stirring at a speed of 200 rpm.

PL450096A 2024-10-22 2024-10-22 Method of immobilizing laccase on the surface of a membrane covered with a gold layer PL450096A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL450096A PL450096A1 (en) 2024-10-22 2024-10-22 Method of immobilizing laccase on the surface of a membrane covered with a gold layer

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL450096A PL450096A1 (en) 2024-10-22 2024-10-22 Method of immobilizing laccase on the surface of a membrane covered with a gold layer

Publications (1)

Publication Number Publication Date
PL450096A1 true PL450096A1 (en) 2026-04-27

Family

ID=99547501

Family Applications (1)

Application Number Title Priority Date Filing Date
PL450096A PL450096A1 (en) 2024-10-22 2024-10-22 Method of immobilizing laccase on the surface of a membrane covered with a gold layer

Country Status (1)

Country Link
PL (1) PL450096A1 (en)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
ADAMIAN Y. I IN.,: "Front. Bioeng. Biotechnol., 2021, 9: 778239.", RECENT DEVELOPMENTS IN THE IMMOBILIZATION OF LACCASE ON CARBONACEOUS SUPPORTS FOR ENVIRONMENTAL APPLICATIONS – A CRITICAL REVIEW, DOI: 10.3389/fbioe.2021.778239 *
HUAJUN QIU I IN.,: "J. Phys. Chem. C, 2009, 113, 6, 2521–2525,", IMMOBILIZATION OF LACCASE ON NANOPOROUS GOLD: COMPARATIVE STUDIES ON THE IMMOBILIZATION STRATEGIES AND THE PARTICLE SIZE EFFECTS, DOI: https://doi.org/10.1021/jp8090304 *
JAKUB ZDARTA I IN.,: "Chemosphere, 2022, 304, 135374", LACCASE IMMOBILIZATION IN POLYELECTROLYTE MULTILAYER MEMBRANES FOR 17Α-ETHYNYLESTRADIOL REMOVAL: BIOCATALYTIC APPROACH FOR PHARMACEUTICALS DEGRADATION,, DOI: doi.org/10.1016/j.chemosphere. *

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