PL450099A1 - Method of immobilizing protease on the surface of a polymer membrane - Google Patents

Method of immobilizing protease on the surface of a polymer membrane

Info

Publication number
PL450099A1
PL450099A1 PL450099A PL45009924A PL450099A1 PL 450099 A1 PL450099 A1 PL 450099A1 PL 450099 A PL450099 A PL 450099A PL 45009924 A PL45009924 A PL 45009924A PL 450099 A1 PL450099 A1 PL 450099A1
Authority
PL
Poland
Prior art keywords
polymer membrane
protease
immobilizing
immobilizing protease
membrane
Prior art date
Application number
PL450099A
Other languages
Polish (pl)
Inventor
Agata Zdarta
Jakub Zdarta
Teofil Jesionowski
Agnieszka Rybarczyk
Original Assignee
Politechnika Poznańska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Poznańska filed Critical Politechnika Poznańska
Priority to PL450099A priority Critical patent/PL450099A1/en
Publication of PL450099A1 publication Critical patent/PL450099A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób immobilizacja proteazy na powierzchni membrany polimerowej polegający na tym, że w reaktorze membranowym instaluje się membranę polimerową pokrytą złotem o powierzchni 4.9 cm2, następnie dodaje się 10 mL roztworu proteazy Aspergillus saitoi w buforze tris-HCl o pH 7,5 i stężeniu w zakresie od 1 do 10 mg/mL, korzystnie 5 mg/mL i prowadzi się proces immobilizacji w czasie 24 godzin przy ciągłym mieszaniu z prędkością 200 rpm.The subject of the application is a method for immobilizing protease on the surface of a polymer membrane, which consists in installing a gold-coated polymer membrane with a surface of 4.9 cm2 in a membrane reactor, then adding 10 mL of Aspergillus saitoi protease solution in a tris-HCl buffer with a pH of 7.5 and a concentration in the range of 1 to 10 mg/mL, preferably 5 mg/mL, and carrying out the immobilization process for 24 hours with continuous stirring at a speed of 200 rpm.

PL450099A 2024-10-22 2024-10-22 Method of immobilizing protease on the surface of a polymer membrane PL450099A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL450099A PL450099A1 (en) 2024-10-22 2024-10-22 Method of immobilizing protease on the surface of a polymer membrane

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL450099A PL450099A1 (en) 2024-10-22 2024-10-22 Method of immobilizing protease on the surface of a polymer membrane

Publications (1)

Publication Number Publication Date
PL450099A1 true PL450099A1 (en) 2026-04-27

Family

ID=99547493

Family Applications (1)

Application Number Title Priority Date Filing Date
PL450099A PL450099A1 (en) 2024-10-22 2024-10-22 Method of immobilizing protease on the surface of a polymer membrane

Country Status (1)

Country Link
PL (1) PL450099A1 (en)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DUAN, C., MEYERHOFF, M.E.: "Mikrochim Acta 117, 195–206 (1995).", IMMOBILIZATION OF PROTEINS ON GOLD COATED POROUS MEMBRANES VIA AN ACTIVATED SELF-ASSEMBLED MONOLAYER OF THIOCTIC ACID, DOI: https://doi.org/10.1007/BF01244890 *
GARIMA GOYAL I IN.: "Sensors and Actuators B: Chemical, Volume 305, 2020, 127442,", PROTEASE FUNCTIONAL ASSAY ON MEMBRANE, DOI: https://doi.org/10.1016/j.snb.2019.127442. *
STEEGMÜLLER T.,: "Membranes, 24.01.2024, 14, 31.", DEVELOPMENT OF A NEW AFFINITY GOLD POLYMER MEMBRANE WITH IMMOBILIZED PROTEIN A, DOI: https:// doi.org/10.3390/membranes14020031. *
SUMANT PHADTARE I IN.: "Biotechnology and Bioengineering, Vol. 85, NO. 6, 2004,", IMMOBILIZATION AND BIOCATALYTIC ACTIVITY OF FUNGAL PROTEASE ON GOLD NANOPARTICLE LOADED ZEOLITE MICROSPHERES, DOI: 10.1002/bit.10856 *

Similar Documents

Publication Publication Date Title
Pavlostathis et al. Kinetics of anaerobic treatment
Boone et al. Diffusion of the interspecies electron carriers H2 and formate in methanogenic ecosystems and its implications in the measurement of K m for H2 or formate uptake
BRPI0516035A (en) method for the treatment of biomass and organic waste for the purpose of generating desired biobased products
CN103180452A (en) Organic acid fermentation method using cellulose-containing waste from rumen juice
Petruccioli et al. Effect of stirrer speed and buffering agents on the production of glucose oxidase and catalase by Penicillium variabile (P16) in benchtop bioreactor
JP2001321792A (en) Anaerobic treatment method and treatment system for oil and fat-containing pollutants
EP0109083B1 (en) Method for cultivation of pseudomonas bacteria
WO2010147111A1 (en) Cell-free protein synthesis solution, cell-free protein synthesis kit, and protein synthesis method
BR0110148A (en) Improved process for converting an aqueous biomass hydrolyzate into fuels or chemicals by selectively removing fermentation inhibitors and fermentable media
PL450099A1 (en) Method of immobilizing protease on the surface of a polymer membrane
PL450097A1 (en) Method of immobilizing cellulase on the surface of a polymer membrane
CN116161780B (en) A method for enhancing the low-temperature denitrification performance of anaerobic ammonium-oxidizing bacteria
PL450108A1 (en) Method of immobilizing protease on the surface of a polymer membrane
PL450098A1 (en) Method of immobilizing lipase on the surface of a polymer membrane
CN109437394B (en) Method for controlling short-cut nitrification by using combined inhibitor
MIAH et al. Enhancement of biogas production from sewage sludge with the addition of Geobacillus sp. strain AT1 culture
Jacobson et al. Control of arylsulfatase in a serine auxotroph of Neurospora
PL450096A1 (en) Method of immobilizing laccase on the surface of a membrane covered with a gold layer
JPH01256382A (en) Heat-stable amylase and pullulanase
PL450107A1 (en) Method of immobilizing cellulase on the surface of an aluminum-coated membrane
PL450106A1 (en) Method of immobilizing laccase on the surface of a membrane covered with an aluminum layer
Paveto et al. A guanine nucleotide-sensitive, glugagon-stimulated adenylyl cyclase in candida albicans: Effect of glucagon on cell morphology
PL450095A1 (en) Method of immobilizing lipase on the surface of a polymer membrane covered with an aluminum layer
CN108504606A (en) A kind of unwrapping wire bacterium culture medium and its application
JPH0630782A (en) Enzyme reaction method