PL450097A1 - Method of immobilizing cellulase on the surface of a polymer membrane - Google Patents

Method of immobilizing cellulase on the surface of a polymer membrane

Info

Publication number
PL450097A1
PL450097A1 PL450097A PL45009724A PL450097A1 PL 450097 A1 PL450097 A1 PL 450097A1 PL 450097 A PL450097 A PL 450097A PL 45009724 A PL45009724 A PL 45009724A PL 450097 A1 PL450097 A1 PL 450097A1
Authority
PL
Poland
Prior art keywords
polymer membrane
cellulase
immobilizing
immobilizing cellulase
membrane
Prior art date
Application number
PL450097A
Other languages
Polish (pl)
Inventor
Agata Zdarta
Jakub Zdarta
Teofil Jesionowski
Agnieszka Rybarczyk
Original Assignee
Politechnika Poznańska
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Politechnika Poznańska filed Critical Politechnika Poznańska
Priority to PL450097A priority Critical patent/PL450097A1/en
Publication of PL450097A1 publication Critical patent/PL450097A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Przedmiotem zgłoszenia jest sposób immobilizacja celulazy na powierzchni membrany polimerowej polegający na tym, że w reaktorze membranowym instaluje się membranę polimerową pokrytą złotem o powierzchni 4,9 cm2, następnie dodaje się 10 mL roztworu celulazy Aspergillus niger w buforze fosforanowym o pH 7 i stężeniu w zakresie od 1 do 10 mg/mL, korzystnie 5 mg/mL prowadzi się proces immobilizacji w czasie 24 godzin przy ciągłym mieszaniu z prędkością 200 rpm.The subject of the application is a method of immobilizing cellulase on the surface of a polymer membrane, which consists in installing a gold-coated polymer membrane with a surface of 4.9 cm2 in a membrane reactor, then adding 10 mL of Aspergillus niger cellulase solution in a phosphate buffer with a pH of 7 and a concentration in the range of 1 to 10 mg/mL, preferably 5 mg/mL, and carrying out the immobilization process for 24 hours with continuous stirring at a speed of 200 rpm.

PL450097A 2024-10-22 2024-10-22 Method of immobilizing cellulase on the surface of a polymer membrane PL450097A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL450097A PL450097A1 (en) 2024-10-22 2024-10-22 Method of immobilizing cellulase on the surface of a polymer membrane

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PL450097A PL450097A1 (en) 2024-10-22 2024-10-22 Method of immobilizing cellulase on the surface of a polymer membrane

Publications (1)

Publication Number Publication Date
PL450097A1 true PL450097A1 (en) 2026-04-27

Family

ID=99547500

Family Applications (1)

Application Number Title Priority Date Filing Date
PL450097A PL450097A1 (en) 2024-10-22 2024-10-22 Method of immobilizing cellulase on the surface of a polymer membrane

Country Status (1)

Country Link
PL (1) PL450097A1 (en)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DUAN, C., MEYERHOFF, M.E.: "Mikrochim Acta 117, 195–206 (1995).", IMMOBILIZATION OF PROTEINS ON GOLD COATED POROUS MEMBRANES VIA AN ACTIVATED SELF-ASSEMBLED MONOLAYER OF THIOCTIC ACID, DOI: https://doi.org/10.1007/BF01244890 *
KAMYAR KHOSHNEVISAN I IN.,: "Magnetochemistry, 2019, 5, 36", RECENT ADVANCES OF CELLULASE IMMOBILIZATION ONTO MAGNETIC NANOPARTICLES: AN UPDATE REVIEW *
POORAKBAR, E., I IN.,: "Protein J 39, 328–336 (2020).", IMMOBILIZATION OF CELLULASE ONTO CORE-SHELL MAGNETIC GOLD NANOPARTICLES FUNCTIONALIZED BY ASPARTIC ACID AND DETERMINATION OF ITS ACTIVITY, DOI: https://doi.org/10.1007/s10930-020-09906-z. *
STEEGMÜLLER, T. I IN.,: "Membranes, 24.01.2024, 14, 31.", DEVELOPMENT OF A NEW AFFINITY GOLD POLYMER MEMBRANE WITH IMMOBILIZED PROTEIN A, DOI: https://doi.org/10.3390/membranes14020031. *

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