PT86881A - Metodo para a purificacao, separacao e hibridacao de acidos nucleicos utilizando suportes policationicos - Google Patents

Metodo para a purificacao, separacao e hibridacao de acidos nucleicos utilizando suportes policationicos

Info

Publication number
PT86881A
PT86881A PT86881A PT8688188A PT86881A PT 86881 A PT86881 A PT 86881A PT 86881 A PT86881 A PT 86881A PT 8688188 A PT8688188 A PT 8688188A PT 86881 A PT86881 A PT 86881A
Authority
PT
Portugal
Prior art keywords
nucleic acids
separation
supports
purification
polycytatic
Prior art date
Application number
PT86881A
Other languages
English (en)
Original Assignee
Lyle J Arnold Jr
Alexander Atkinson Waldrop Iii
Nelson Norman C
Mark A Reynolds
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lyle J Arnold Jr, Alexander Atkinson Waldrop Iii, Nelson Norman C, Mark A Reynolds filed Critical Lyle J Arnold Jr
Publication of PT86881A publication Critical patent/PT86881A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Cleaning Or Drying Semiconductors (AREA)
  • Detergent Compositions (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Cleaning And De-Greasing Of Metallic Materials By Chemical Methods (AREA)
  • Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
  • Extraction Or Liquid Replacement (AREA)
PT86881A 1987-03-02 1988-03-02 Metodo para a purificacao, separacao e hibridacao de acidos nucleicos utilizando suportes policationicos PT86881A (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2086687A 1987-03-02 1987-03-02

Publications (1)

Publication Number Publication Date
PT86881A true PT86881A (pt) 1989-03-30

Family

ID=21801014

Family Applications (1)

Application Number Title Priority Date Filing Date
PT86881A PT86881A (pt) 1987-03-02 1988-03-02 Metodo para a purificacao, separacao e hibridacao de acidos nucleicos utilizando suportes policationicos

Country Status (12)

Country Link
EP (1) EP0281390B1 (pt)
JP (1) JP2862547B2 (pt)
KR (1) KR960000479B1 (pt)
AT (1) ATE107654T1 (pt)
CA (1) CA1339446C (pt)
DE (1) DE3850273T2 (pt)
DK (1) DK607588A (pt)
ES (1) ES2054797T3 (pt)
FI (1) FI885022A0 (pt)
NO (1) NO884847L (pt)
PT (1) PT86881A (pt)
WO (1) WO1988006633A1 (pt)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310650A (en) * 1986-09-29 1994-05-10 Abbott Laboratoires Method and device for improved reaction kinetics in nucleic acid hybridizations
US6270961B1 (en) * 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US5512439A (en) * 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
WO1990006045A2 (en) * 1988-11-21 1990-06-14 Dynal As Nucleic acid probes
CA2012984A1 (en) * 1989-03-27 1990-09-27 Frank W. Hobbs Jr. Process for rapid nucleic acid detection by incorporating a reporter moiety into amplified target nucleic acid followed by affinity capture
DE69027910T2 (de) * 1989-05-31 1997-02-20 Amoco Corp Nukleinsäuresonden zum Nachweis von Chlamydia trachomatis und Chlamydia psittaci
CA1337639C (en) * 1989-08-01 1995-11-28 Joseph Eugene Celebuski Dna probe assay using neutrally charged probe strands
US5523231A (en) * 1990-02-13 1996-06-04 Amersham International Plc Method to isolate macromolecules using magnetically attractable beads which do not specifically bind the macromolecules
GB9003253D0 (en) * 1990-02-13 1990-04-11 Amersham Int Plc Precipitating polymers
FR2666096B1 (fr) * 1990-08-21 1993-12-31 Sfri Sa Nouveau procede de dosage d'acide desoxyribonucleique present en position extracellulaire dans un milieu.
DE4041080A1 (de) 1990-12-21 1992-06-25 Behringwerke Ag Verfahren zur bestimmung eines analyten
US6100099A (en) * 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
US6093538A (en) * 1992-05-06 2000-07-25 Gen-Probe Incorporated Nucleic acid probes to ureaplasma
WO1994028174A1 (en) * 1993-05-24 1994-12-08 Amoco Corporation Nucleic acid probes for bacteria of the genus legionella
GB9314249D0 (en) * 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
US5438127A (en) * 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane
US5610287A (en) * 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
DE69535240T2 (de) * 1994-10-28 2007-06-06 Gen-Probe Inc., San Diego Zusammensetzungen und Verfahren für die gleichzeitige Detektion und Quantifizierung von einer Mehrheit spezifischer Nuklein Säure Sequenzen
US5635349A (en) * 1994-12-02 1997-06-03 Tularik, Inc. High-throughput screening assay for inhibitors of nucleic acid polymerases
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
DE19520398B4 (de) † 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
KR100463475B1 (ko) 1995-06-08 2005-06-22 로셰 디아그노스틱스 게엠베하 자기성피그먼트
DE19534122A1 (de) * 1995-09-14 1997-03-20 Behringwerke Ag Homogener Gensondentest mit einem gegen das Label gerichteten Rezeptor
US5783686A (en) * 1995-09-15 1998-07-21 Beckman Instruments, Inc. Method for purifying nucleic acids from heterogenous mixtures
US5795784A (en) 1996-09-19 1998-08-18 Abbott Laboratories Method of performing a process for determining an item of interest in a sample
US5856194A (en) 1996-09-19 1999-01-05 Abbott Laboratories Method for determination of item of interest in a sample
DE19736366A1 (de) 1997-08-21 1999-02-25 Ernst Prof Dr Bayer Verfahren zur Isolierung von anionischen organischen Verbindungen aus wäßrigen Systemen mit kationischen Polymernanopartikeln
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US5817798A (en) * 1997-09-17 1998-10-06 Abbott Laboratories Rapid RNA isolation procedure in the presence of a transition metal ion
DE19743518A1 (de) * 1997-10-01 1999-04-15 Roche Diagnostics Gmbh Automatisierbare universell anwendbare Probenvorbereitungsmethode
GB2346615B (en) 1998-11-17 2003-10-15 Cambridge Molecular Tech Isolating nucleic acid
DE19937607A1 (de) * 1999-08-09 2001-02-15 Bilatec Ges Zur Entwicklung Bi Reagenzienkit zur Isolierung von Nukleinsäuren
DE10006590B4 (de) * 2000-02-11 2007-10-18 Qiagen North American Holdings, Inc. Verwendung funktionalisierter Membranen bzw. Matrizes zur Aufreinigung von Nukleinsäuren sowie entsprechende Verfahren
JP4519247B2 (ja) * 2000-03-09 2010-08-04 シスメックス株式会社 核酸の抽出精製用試薬
US6692700B2 (en) 2001-02-14 2004-02-17 Handylab, Inc. Heat-reduction methods and systems related to microfluidic devices
WO2004060278A2 (en) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Methods for rapid identification of pathogens in humans and animals
US20040121309A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in blood, bodily fluids, and bodily tissues
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US7718354B2 (en) 2001-03-02 2010-05-18 Ibis Biosciences, Inc. Methods for rapid identification of pathogens in humans and animals
US6852287B2 (en) 2001-09-12 2005-02-08 Handylab, Inc. Microfluidic devices having a reduced number of input and output connections
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US8895311B1 (en) 2001-03-28 2014-11-25 Handylab, Inc. Methods and systems for control of general purpose microfluidic devices
US7829025B2 (en) 2001-03-28 2010-11-09 Venture Lending & Leasing Iv, Inc. Systems and methods for thermal actuation of microfluidic devices
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
CA2473376A1 (en) 2002-01-16 2003-07-31 Invitrogen Dynal As Method for isolating nucleic acids and protein from a single sample
SE0202074D0 (sv) * 2002-06-28 2002-07-02 Amersham Biosciences Ab Isolation of nucleic acids
JP4437218B2 (ja) * 2002-11-26 2010-03-24 日生バイオ株式会社 Rna−プロタミン複合体、その製造方法およびそれを含む健康食品
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
US8046171B2 (en) 2003-04-18 2011-10-25 Ibis Biosciences, Inc. Methods and apparatus for genetic evaluation
EP1654066B1 (en) 2003-07-31 2014-11-12 Handylab, Inc. Processing particle-containing samples
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
US8119336B2 (en) 2004-03-03 2012-02-21 Ibis Biosciences, Inc. Compositions for use in identification of alphaviruses
EP2345739B8 (en) * 2004-05-03 2016-12-07 Handylab, Inc. A microfluidic device for processing polynucleotide-containing samples
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
ES2641832T3 (es) 2004-05-24 2017-11-14 Ibis Biosciences, Inc. Espectrometría de masas con filtración de iones selectiva por establecimiento de umbrales digitales
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
DE102005060392A1 (de) 2005-12-16 2007-06-21 Süd-Chemie AG Verfahren zur Abtrennung von Proteinen aus flüssigen Medien
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
DK2001990T3 (en) 2006-03-24 2016-10-03 Handylab Inc Integrated microfluidic sample processing system and method for its use
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP2079838A1 (en) * 2006-05-16 2009-07-22 NuGEN Technologies, Inc. Nucleic acid separation and purification method based on reversible charge interactions
AU2007353877B2 (en) 2006-09-14 2012-07-19 Ibis Biosciences, Inc. Targeted whole genome amplification method for identification of pathogens
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2008061165A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic cartridge and method of making same
JP5680304B2 (ja) 2007-02-23 2015-03-04 アイビス バイオサイエンシズ インコーポレイティッド 迅速な法医学的dna分析法
CA2629589C (en) 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US8324372B2 (en) 2007-07-13 2012-12-04 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8133671B2 (en) 2007-07-13 2012-03-13 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US9618139B2 (en) 2007-07-13 2017-04-11 Handylab, Inc. Integrated heater and magnetic separator
JP4545200B2 (ja) * 2008-02-04 2010-09-15 積水メディカル株式会社 核酸固定用磁性粒子
USD787087S1 (en) 2008-07-14 2017-05-16 Handylab, Inc. Housing
US8534447B2 (en) 2008-09-16 2013-09-17 Ibis Biosciences, Inc. Microplate handling systems and related computer program products and methods
WO2010033627A2 (en) 2008-09-16 2010-03-25 Ibis Biosciences, Inc. Sample processing units, systems, and related methods
EP2396803A4 (en) 2009-02-12 2016-10-26 Ibis Biosciences Inc IONIZATION PROBE ASSEMBLIES
US8950604B2 (en) 2009-07-17 2015-02-10 Ibis Biosciences, Inc. Lift and mount apparatus
EP2454000A4 (en) 2009-07-17 2016-08-10 Ibis Biosciences Inc SYSTEMS FOR IDENTIFYING BIOLOGICAL SUBSTANCES
EP2957641B1 (en) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple displacement amplification
EP2697657B8 (en) 2011-04-15 2017-08-16 Becton, Dickinson and Company Scanning real-time microfluidic thermocycler and methods for synchronized thermocycling and scanning optical detection
USD692162S1 (en) 2011-09-30 2013-10-22 Becton, Dickinson And Company Single piece reagent holder
DK3273253T3 (da) 2011-09-30 2020-10-12 Becton Dickinson Co Forenet reagensstrimmel
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
DK2810080T3 (da) 2012-02-03 2024-06-17 Becton Dickinson Co Eksterne filer til fordeling af molekylære diagnostiske tests og bestemmelse af kompatabilitet imellem tests

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NL8203102A (nl) * 1982-08-04 1984-03-01 Katholieke Universiteit Facult Papier voor rna-blotting; werkwijze voor het maken daarvan; toepassingen van het papier.
US4672040A (en) * 1983-05-12 1987-06-09 Advanced Magnetics, Inc. Magnetic particles for use in separations
US4554088A (en) * 1983-05-12 1985-11-19 Advanced Magnetics Inc. Magnetic particles for use in separations
US4652517A (en) * 1984-06-11 1987-03-24 Diagnostic Research Limited Partnership Methods for the in vitro detection and identification of unknown pathogens or genetic entities
EP0189280A3 (en) * 1985-01-23 1987-01-07 Dekalb-Pfizer Genetics Qualitative testing for nucleic acid
WO1986005519A1 (en) * 1985-03-15 1986-09-25 James Summerton Polynucleotide assay reagent and method
US4806631A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on solvolyzed nylon supports
US4806546A (en) * 1985-09-30 1989-02-21 Miles Inc. Immobilization of nucleic acids on derivatized nylon supports

Also Published As

Publication number Publication date
DE3850273D1 (de) 1994-07-28
ATE107654T1 (de) 1994-07-15
FI885022A7 (fi) 1988-11-01
ES2054797T3 (es) 1994-08-16
NO884847L (no) 1988-12-22
KR960000479B1 (ko) 1996-01-08
JP2862547B2 (ja) 1999-03-03
CA1339446C (en) 1997-09-09
DK607588A (da) 1988-12-28
AU618951B2 (en) 1992-01-16
DK607588D0 (da) 1988-11-01
FI885022L (fi) 1988-11-01
KR890700682A (ko) 1989-04-26
WO1988006633A1 (en) 1988-09-07
EP0281390B1 (en) 1994-06-22
EP0281390A3 (en) 1990-04-25
FI885022A0 (fi) 1988-11-01
EP0281390A2 (en) 1988-09-07
DE3850273T2 (de) 1994-09-29
NO884847D0 (no) 1988-10-31
AU1426988A (en) 1988-09-26
JPH01502319A (ja) 1989-08-17

Similar Documents

Publication Publication Date Title
ATE107654T1 (de) Polykationische träger zur reinigung, trennung und hybridisierung von nukleinsäure.
GR3003056T3 (en) Method for increasing the sensitivity of nucleic acid hybridization assays
DE60039037D1 (de) Methode zur direkten sequenzierung von nukleinsäuren
ATE159762T1 (de) Verfahren zur dns-sequenzbestimmung mit dns- polymerase von thermus aquaticus
ATE192503T1 (de) Manipulation von nukleinsäuresequenzen
ES2085245T3 (es) Procedimiento de purificacion de acidos nucleicos.
DE69034177D1 (de) Verfahren zur Amplifikation von Nukleinsäuresequenzen
DE3750774D1 (de) Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe.
ATE147791T1 (de) Festphasendiagnose von medizinischen konditionen
DK1157264T3 (da) Biokemiske oprensningsindretninger med immobiliserede opfangningsprober og anvendelser deraf
DE69432419D1 (de) Ein elektrochemiluminescenter nachweis von nukleinsäuren auf basis der selbsttragenden sequenzreplikation
DE3689412D1 (de) Verfahren zur durchführung von hybridisierungstests von nukleinsäuren.
DK551088A (da) Hybridiseringsmetode og udstyr og reagenssaet til anvendelse ved hybridiseringen
EP1411340A3 (en) Biochemical purification devices with immobilized capture probes and their uses
ES2052607T3 (es) Metodos y kits para detectar secuencias de acidos nucleicos.
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
ATE144557T1 (de) Nucleinsäure-proben und verfahren zum nachweis von giardia lamblia
ATE123315T1 (de) Verfahren zur reinigung, vervielfachung und zum nachweis einer nukleinsäure.
EP0233257A4 (en) Method of attaching biological substances to solid substrates.
Katō Progress in HPLC.: Gel permeation and ion-exchange chromatography of proteins and peptides
ATE118823T1 (de) Schnelles verfahren zur identifizierung einer spezifischen sequenz von nukleinsäure.
DE3882674D1 (de) Verfahren zur bindung von biologischen reagenzien an poroese traeger.
GR3005459T3 (pt)
ATE98701T1 (de) Verfahren zur durchfuehrung von hybridisierungstests von nukleinsaeuren.

Legal Events

Date Code Title Description
FC3A Refusal

Effective date: 19930326