UA48951C2 - Ізольований фрагмент днк промотору гена синтази ацетооксикислот (ahas) для експресії генів в рослинах, вектор для трансформації рослин, спосіб високорівневого експресування гетерологічного гена в рослині та в різноманітних тканинах цієї рослини, конструкція нуклеїнової кислоти, спосіб селекції трансгенного рослинного матеріалу - Google Patents
Ізольований фрагмент днк промотору гена синтази ацетооксикислот (ahas) для експресії генів в рослинах, вектор для трансформації рослин, спосіб високорівневого експресування гетерологічного гена в рослині та в різноманітних тканинах цієї рослини, конструкція нуклеїнової кислоти, спосіб селекції трансгенного рослинного матеріалуInfo
- Publication number
- UA48951C2 UA48951C2 UA97041679A UA97041679A UA48951C2 UA 48951 C2 UA48951 C2 UA 48951C2 UA 97041679 A UA97041679 A UA 97041679A UA 97041679 A UA97041679 A UA 97041679A UA 48951 C2 UA48951 C2 UA 48951C2
- Authority
- UA
- Ukraine
- Prior art keywords
- plants
- plant
- ahas
- heterologous gene
- transformation
- Prior art date
Links
- 239000012634 fragment Substances 0.000 title abstract 6
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 108010000700 Acetolactate synthase Proteins 0.000 title abstract 2
- 230000014509 gene expression Effects 0.000 title abstract 2
- 239000013598 vector Substances 0.000 title abstract 2
- 239000002253 acid Substances 0.000 title 1
- 230000009466 transformation Effects 0.000 title 1
- 230000009261 transgenic effect Effects 0.000 title 1
- 235000013311 vegetables Nutrition 0.000 title 1
- 240000008042 Zea mays Species 0.000 abstract 3
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 abstract 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 3
- 235000009973 maize Nutrition 0.000 abstract 3
- 241000196324 Embryophyta Species 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 abstract 1
- 102000053187 Glucuronidase Human genes 0.000 abstract 1
- 108010060309 Glucuronidase Proteins 0.000 abstract 1
- 108091092724 Noncoding DNA Proteins 0.000 abstract 1
- 108700008625 Reporter Genes Proteins 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Цей винахід відноситься до ізольованих фрагментів ДНК, що не кодують, корисним для використання в якості промоторів для експресії гетерологічних генів у рослин. Дійсний винахід також відноситься до векторів, що включають ці ізольовані нуклеотидні послідовності. Для експресії інтродуційованих генів на високому рівні та у різних тканинах рослин використовуються промотори гена синтази ацетооксикислот (AHAS) з кукурудзи. Були клоновані і секвеновані промотори з als1 та als2 генів кукурудзи, і регіони промоторів цих генів потім введені в плазміду 5' до гена-репортера β-глюкуронідази (GUS). Обидва фрагменти промотору походять з лінії кукурудзи XI12. Фрагмент промотору als1 довжиною приблизно 1400 пар основ, у той час як промотор als2 містить 819 пар основ.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/303,266 US5750866A (en) | 1994-09-08 | 1994-09-08 | AHAS promoter useful for expression of introduced genes in plants |
| PCT/US1995/011199 WO1996007746A1 (en) | 1994-09-08 | 1995-09-05 | Acetohydroxyacid synthase promoter expression of introduced genes in plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| UA48951C2 true UA48951C2 (uk) | 2002-09-16 |
Family
ID=23171273
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| UA97041679A UA48951C2 (uk) | 1994-09-08 | 1995-09-05 | Ізольований фрагмент днк промотору гена синтази ацетооксикислот (ahas) для експресії генів в рослинах, вектор для трансформації рослин, спосіб високорівневого експресування гетерологічного гена в рослині та в різноманітних тканинах цієї рослини, конструкція нуклеїнової кислоти, спосіб селекції трансгенного рослинного матеріалу |
Country Status (26)
| Country | Link |
|---|---|
| US (2) | US5750866A (uk) |
| EP (1) | EP0779928B1 (uk) |
| JP (1) | JP3655922B2 (uk) |
| KR (1) | KR970705637A (uk) |
| CN (1) | CN1210404C (uk) |
| AT (1) | ATE291631T1 (uk) |
| AU (1) | AU701239B2 (uk) |
| BG (1) | BG64062B1 (uk) |
| BR (1) | BR9509202A (uk) |
| CA (1) | CA2198497C (uk) |
| CZ (1) | CZ69597A3 (uk) |
| DE (1) | DE69534101T2 (uk) |
| EE (1) | EE03933B1 (uk) |
| ES (1) | ES2240978T3 (uk) |
| FI (1) | FI119818B (uk) |
| HU (1) | HU221795B1 (uk) |
| MD (1) | MD2312B2 (uk) |
| MX (1) | MX9701684A (uk) |
| NO (1) | NO323171B1 (uk) |
| NZ (1) | NZ293093A (uk) |
| PL (1) | PL188062B1 (uk) |
| RO (1) | RO117190B1 (uk) |
| RU (1) | RU2197527C2 (uk) |
| SK (1) | SK30697A3 (uk) |
| UA (1) | UA48951C2 (uk) |
| WO (1) | WO1996007746A1 (uk) |
Families Citing this family (56)
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|---|---|---|---|---|
| US5659026A (en) * | 1995-03-24 | 1997-08-19 | Pioneer Hi-Bred International | ALS3 promoter |
| ATE342985T1 (de) * | 1998-02-26 | 2006-11-15 | Pioneer Hi Bred Int | Mais alpha-tubulin 3-18 promoter |
| EP1462522B1 (en) * | 1998-02-26 | 2006-08-16 | Pioneer Hi-Bred International, Inc. | Maize Met-1 promoter |
| US6504083B1 (en) * | 1998-10-06 | 2003-01-07 | Pioneer Hi-Bred International, Inc. | Maize Gos-2 promoters |
| US6528701B1 (en) | 1999-03-02 | 2003-03-04 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Rice ubiquitin-derived promoters |
| US7122721B1 (en) | 1999-10-05 | 2006-10-17 | Basf Aktiengesellschaft | Plant gene expression under the control of constitutive plant V-ATPase promoters |
| WO2001082685A1 (en) | 2000-04-28 | 2001-11-08 | Basf Aktiengesellschaft | Use of the maize x112 mutant ahas 2 gene and imidazolinone herbicides for selection of transgenic monocots, maize, rice and wheat plants resistant to the imidazolinone herbicides |
| EP2292768A1 (en) | 2002-07-09 | 2011-03-09 | BASF Plant Science GmbH | Use of AHAS mutant genes as selection marker in potato transformation |
| CA2494626A1 (en) | 2002-08-07 | 2004-03-04 | Basf Plant Science Gmbh | Nucleic acid sequences encoding proteins associated with abiotic stress response |
| CA2516042A1 (en) | 2003-02-17 | 2004-09-02 | Metanomics Gmbh | Preparation of transgenic plants comprising a nucleic acid molecule encoding an l450 polypeptide-exhibiting faster growth and/or increased yield |
| EP1641930B1 (en) | 2003-04-15 | 2011-06-29 | BASF Plant Science GmbH | Nucleic acid sequences encoding proteins associated with abiotic stress response and plant cells and plants with increased tolerance to environmental stress |
| WO2005014828A2 (en) | 2003-08-01 | 2005-02-17 | Basf Plant Science Gmbh | Process for the production of fine chemicals in plants |
| EP1491634A1 (en) * | 2003-06-26 | 2004-12-29 | Degussa AG | Feedback resistant acetohydroxy acid synthetase mutants |
| EP1765857A2 (en) | 2004-07-02 | 2007-03-28 | Metanomics GmbH | Process for the production of fine chemicals |
| AU2005268943B2 (en) | 2004-07-31 | 2011-04-14 | Metanomics Gmbh | Preparation of organisms with faster growth and/or higher yield |
| CN103289961A (zh) | 2004-09-24 | 2013-09-11 | 巴斯福植物科学有限公司 | 编码与非生物性胁迫反应相关的蛋白质的核酸序列和环境胁迫抗性增加的植物细胞和植物 |
| AU2005286427B2 (en) | 2004-09-24 | 2011-09-15 | Basf Plant Science Gmbh | Plant cells and plants with increased tolerance to environmental stress |
| CA2585798A1 (en) | 2004-12-17 | 2006-06-17 | Metanomics Gmbh | Process for the control of production of fine chemicals |
| WO2006092449A2 (en) * | 2005-03-02 | 2006-09-08 | Metanomics Gmbh | Process for the production of fine chemicals |
| EP2573188A2 (en) | 2005-03-02 | 2013-03-27 | Metanomics GmbH | Process for the production of fine chemicals |
| US7737326B2 (en) * | 2005-04-29 | 2010-06-15 | Midwest Oil Seeds Inc. | EPSPS promoter from maize |
| MX2008012252A (es) | 2006-03-24 | 2009-01-14 | Basf Plant Science Gmbh | Proteinas que se relacionan con la respuesta al estres abiotico y homologos. |
| CA2644273A1 (en) | 2006-04-05 | 2008-03-27 | Metanomics Gmbh | Process for the production of a fine chemical |
| MX2008015090A (es) | 2006-05-31 | 2008-12-15 | Metanomics Gmbh | Manipulacion del metabolismo del nitrogeno. |
| US8344205B2 (en) | 2006-10-13 | 2013-01-01 | Basf Plant Science Gmbh | Plants with increased yield |
| US20080176225A1 (en) * | 2007-01-18 | 2008-07-24 | Steve Roffler | Membrane bound reporter gene system |
| UA126227C2 (uk) | 2007-04-04 | 2022-09-07 | Басф Плант Саєнс Гмбх | Злакова рослина, толерантна до імідозолінону або сульфонілсечовини |
| US20100162432A1 (en) | 2007-05-22 | 2010-06-24 | Basf Plant Science Gmbh | Plant cells and plants with increased tolerance and/or resistance to environmental stress and increased biomass production-ko |
| AR067318A1 (es) | 2007-05-22 | 2009-10-07 | Basf Plant Science Gmbh | Plantas con mayor tolerancia y/o resistencia aumentada al estres ambiental y mayor produccion de biomasa |
| AU2008300579B2 (en) | 2007-09-18 | 2014-11-13 | Basf Plant Science Gmbh | Plants with increased yield |
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| EP2352822A2 (en) | 2008-08-19 | 2011-08-10 | BASF Plant Science GmbH | Plants with increased yield by increasing or generating one or more activities in a plant or a part thereof |
| BRPI0919153A2 (pt) | 2008-09-23 | 2019-05-07 | Basf Plant Science Gmbh | método para produzir uma planta com rendimento aumentado, molécula de ácido nucleico, construto de ácido nucleico, vetor, processo para produzir um polipeptídeo, poliptídeo, anticorpo, núcleo de célula de planta, célula de planta, tecido de planta, material de propagação, pólen, progênie, material coletado, ou uma planta, ou uma parte da planta, planta transgênica, processo para identificar um composto, método para a produção de uma composição agrícola, composição, uso de ácido nucleico, e, método para a identificação de uma planta com um rendimento aumentado, e para aumentar o rendimento de uma população de plantas |
| CN104745624A (zh) | 2008-10-23 | 2015-07-01 | 巴斯夫植物科学有限公司 | 生产具有增加的γ-氨基丁酸(GABA)含量的转基因细胞的方法 |
| MX2011004270A (es) | 2008-10-23 | 2011-07-13 | Basf Plant Science Gmbh | Plantas con rendimiento aumentado (nue). |
| CN101475941B (zh) * | 2008-11-28 | 2011-09-07 | 北京市农林科学院 | 一种玉米Zmptil基因启动子及其应用 |
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| CN109468339B (zh) | 2009-08-31 | 2022-11-04 | 巴斯夫植物科学有限公司 | 用于增强植物中组成型基因表达的调节性核酸分子 |
| CA2772267A1 (en) | 2009-08-31 | 2011-03-03 | Basf Plant Science Company Gmbh | Regulatory nucleic acid molecules for enhancing seed-specific gene expression in plants promoting enhanced polyunsaturated fatty acid synthesis |
| EP3156491B1 (en) * | 2009-08-31 | 2019-10-09 | BASF Plant Science Company GmbH | Regulatory nucleic acid molecules for enhancing seed-specific and/or seed-preferential gene expression in plants |
| EP2319872A1 (en) | 2009-11-04 | 2011-05-11 | BASF Plant Science GmbH | Amylopectin type starch with enhanced retrogradation stability |
| AU2010320547B2 (en) | 2009-11-17 | 2016-06-09 | Basf Plant Science Company Gmbh | Plants with increased yield |
| EP2797406B1 (en) * | 2011-12-30 | 2020-01-08 | Dow AgroSciences LLC | Construct and method for synthetic bidirectional plant promoter ubi1 |
| KR20140109909A (ko) * | 2011-12-30 | 2014-09-16 | 다우 아그로사이언시즈 엘엘씨 | 합성 양방향성 scbv 식물 프로모터를 위한 방법 및 구축물 |
| CN102676549B (zh) * | 2012-01-09 | 2014-04-09 | 中国中医科学院中药研究所 | 一个参与丹参酮生物合成的cyp450基因及其编码产物与应用 |
| US9441238B2 (en) | 2012-04-27 | 2016-09-13 | Synthetic Genomics, Inc. | Compositions and methods for modulating the sensitivity of cells to AHAS inhibitors |
| US10462994B2 (en) | 2013-01-29 | 2019-11-05 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing HCP7 |
| WO2014118018A1 (en) | 2013-01-29 | 2014-08-07 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing ein2 |
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| CN105008542B (zh) | 2013-03-08 | 2020-02-07 | 巴斯夫植物科学有限公司 | 表达MybTF的抗真菌性植物 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4943674A (en) * | 1987-05-26 | 1990-07-24 | Calgene, Inc. | Fruit specific transcriptional factors |
| US5013659A (en) * | 1987-07-27 | 1991-05-07 | E. I. Du Pont De Nemours And Company | Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase |
| EP0360750A3 (en) * | 1988-09-22 | 1991-01-02 | Ciba-Geigy Ag | Novel herbicide tolerant plants |
| DE69126474T2 (de) * | 1990-05-09 | 1997-12-11 | American Cyanamid Co | Verfahren zur Verhinderung von Ernteschäden verursacht durch synergistische Pestizidkombinationen |
| GB9024728D0 (en) * | 1990-11-14 | 1991-01-02 | Ici Plc | Herbicide resistant plants |
| TW208716B (uk) * | 1990-12-27 | 1993-07-01 | American Cyanamid Co | |
| US5731180A (en) * | 1991-07-31 | 1998-03-24 | American Cyanamid Company | Imidazolinone resistant AHAS mutants |
-
1994
- 1994-09-08 US US08/303,266 patent/US5750866A/en not_active Expired - Lifetime
-
1995
- 1995-09-05 CN CNB951949802A patent/CN1210404C/zh not_active Expired - Lifetime
- 1995-09-05 BR BR9509202A patent/BR9509202A/pt not_active IP Right Cessation
- 1995-09-05 UA UA97041679A patent/UA48951C2/uk unknown
- 1995-09-05 MX MX9701684A patent/MX9701684A/es active IP Right Grant
- 1995-09-05 MD MD97-0144A patent/MD2312B2/ro unknown
- 1995-09-05 CZ CZ97695A patent/CZ69597A3/cs unknown
- 1995-09-05 SK SK306-97A patent/SK30697A3/sk unknown
- 1995-09-05 ES ES95932390T patent/ES2240978T3/es not_active Expired - Lifetime
- 1995-09-05 DE DE69534101T patent/DE69534101T2/de not_active Expired - Lifetime
- 1995-09-05 NZ NZ293093A patent/NZ293093A/xx unknown
- 1995-09-05 EE EE9700213A patent/EE03933B1/xx not_active IP Right Cessation
- 1995-09-05 RU RU97106101/13A patent/RU2197527C2/ru active
- 1995-09-05 WO PCT/US1995/011199 patent/WO1996007746A1/en not_active Ceased
- 1995-09-05 EP EP95932390A patent/EP0779928B1/en not_active Expired - Lifetime
- 1995-09-05 JP JP50961796A patent/JP3655922B2/ja not_active Expired - Lifetime
- 1995-09-05 KR KR1019970701508A patent/KR970705637A/ko not_active Ceased
- 1995-09-05 AU AU35448/95A patent/AU701239B2/en not_active Expired
- 1995-09-05 HU HU9701927A patent/HU221795B1/hu active IP Right Grant
- 1995-09-05 RO RO97-00422A patent/RO117190B1/ro unknown
- 1995-09-05 CA CA002198497A patent/CA2198497C/en not_active Expired - Lifetime
- 1995-09-05 AT AT95932390T patent/ATE291631T1/de active
- 1995-09-05 PL PL95319053A patent/PL188062B1/pl unknown
-
1997
- 1997-02-27 BG BG101270A patent/BG64062B1/bg unknown
- 1997-03-06 FI FI970958A patent/FI119818B/fi not_active IP Right Cessation
- 1997-03-07 NO NO19971069A patent/NO323171B1/no not_active IP Right Cessation
- 1997-05-23 US US08/862,881 patent/US6025541A/en not_active Expired - Lifetime
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