SE501439C2 - Sätt och anordning för analys av polynukleotidsekvenser - Google Patents

Sätt och anordning för analys av polynukleotidsekvenser

Info

Publication number: SE501439C2
Authority: SE; Sweden
Prior art keywords: polynucleotide; sequence; sequences; base; terminating
Prior art date: 1993-06-22

Application number

SE9302152A

Other languages

English (en)

Swedish (sv)

Other versions

SE9302152L (sv

SE9302152D0 (sv

Inventor

C Thomas Caskey

Original Assignee

Pharmacia Lkb Biotech

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1993-06-22

Filing date

1993-06-22

Publication date

1995-02-13

1993-06-22 Application filed by Pharmacia Lkb Biotech filed Critical Pharmacia Lkb Biotech

1993-06-22 Priority to SE9302152A priority Critical patent/SE501439C2/sv

1993-06-22 Publication of SE9302152D0 publication Critical patent/SE9302152D0/xx

1994-06-22 Priority to AT94921363T priority patent/ATE185843T1/de

1994-06-22 Priority to PCT/US1994/007086 priority patent/WO1995000669A1/en

1994-06-22 Priority to ES94921363T priority patent/ES2141828T3/es

1994-06-22 Priority to US08/564,100 priority patent/US6153379A/en

1994-06-22 Priority to EP94921363A priority patent/EP0705349B1/de

1994-06-22 Priority to CA002164715A priority patent/CA2164715A1/en

1994-06-22 Priority to EP99200676A priority patent/EP0969103A3/de

1994-06-22 Priority to DE69421277T priority patent/DE69421277T2/de

1994-06-22 Priority to JP50308095A priority patent/JP3722833B2/ja

1994-06-22 Priority to AU72121/94A priority patent/AU698553B2/en

1994-12-23 Publication of SE9302152L publication Critical patent/SE9302152L/xx

1995-02-13 Publication of SE501439C2 publication Critical patent/SE501439C2/sv

2000-11-13 Priority to US09/711,476 priority patent/US7001722B1/en

2002-09-25 Priority to US10/254,828 priority patent/US20030082613A1/en

Links

108091033319 polynucleotide Proteins 0.000 title claims abstract description 33
102000040430 polynucleotide Human genes 0.000 title claims abstract description 33
239000002157 polynucleotide Substances 0.000 title claims abstract description 33
238000000034 method Methods 0.000 title claims abstract description 19
239000002773 nucleotide Substances 0.000 claims abstract description 20
125000003729 nucleotide group Chemical group 0.000 claims abstract description 20
239000012634 fragment Substances 0.000 claims abstract description 11
238000006243 chemical reaction Methods 0.000 claims abstract description 6
238000009396 hybridization Methods 0.000 claims abstract description 3
108091034117 Oligonucleotide Proteins 0.000 claims description 20
239000007790 solid phase Substances 0.000 claims description 19
238000004458 analytical method Methods 0.000 claims description 10
YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 8
108010090804 Streptavidin Proteins 0.000 claims description 5
229960002685 biotin Drugs 0.000 claims description 4
235000020958 biotin Nutrition 0.000 claims description 4
239000011616 biotin Substances 0.000 claims description 4
239000000126 substance Substances 0.000 claims description 3
239000005546 dideoxynucleotide Substances 0.000 claims description 2
239000011541 reaction mixture Substances 0.000 claims description 2
230000009870 specific binding Effects 0.000 claims description 2
108090001008 Avidin Proteins 0.000 claims 1
239000007787 solid Substances 0.000 abstract description 2
239000003155 DNA primer Substances 0.000 abstract 3
238000000137 annealing Methods 0.000 abstract 1
239000013615 primer Substances 0.000 abstract 1
230000035772 mutation Effects 0.000 description 18
108020004414 DNA Proteins 0.000 description 15
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 9
238000007792 addition Methods 0.000 description 8
239000003550 marker Substances 0.000 description 6
238000003776 cleavage reaction Methods 0.000 description 4
239000000463 material Substances 0.000 description 4
230000007017 scission Effects 0.000 description 4
108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
108091028043 Nucleic acid sequence Proteins 0.000 description 3
238000001514 detection method Methods 0.000 description 3
239000011521 glass Substances 0.000 description 3
239000000203 mixture Substances 0.000 description 3
108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 2
238000012300 Sequence Analysis Methods 0.000 description 2
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 2
230000027455 binding Effects 0.000 description 2
230000008878 coupling Effects 0.000 description 2
238000010168 coupling process Methods 0.000 description 2
238000005859 coupling reaction Methods 0.000 description 2
230000037430 deletion Effects 0.000 description 2
238000012217 deletion Methods 0.000 description 2
230000002255 enzymatic effect Effects 0.000 description 2
125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
238000000926 separation method Methods 0.000 description 2
229910052717 sulfur Inorganic materials 0.000 description 2
239000011593 sulfur Substances 0.000 description 2
108020004774 Alkaline Phosphatase Proteins 0.000 description 1
102000002260 Alkaline Phosphatase Human genes 0.000 description 1
201000003883 Cystic fibrosis Diseases 0.000 description 1
102000053602 DNA Human genes 0.000 description 1
238000001712 DNA sequencing Methods 0.000 description 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
206010059866 Drug resistance Diseases 0.000 description 1
102000004190 Enzymes Human genes 0.000 description 1
108090000790 Enzymes Proteins 0.000 description 1
108090000371 Esterases Proteins 0.000 description 1
108700024394 Exon Proteins 0.000 description 1
208000015872 Gaucher disease Diseases 0.000 description 1
208000026350 Inborn Genetic disease Diseases 0.000 description 1
102100034343 Integrase Human genes 0.000 description 1
208000024556 Mendelian disease Diseases 0.000 description 1
229910019142 PO4 Inorganic materials 0.000 description 1
108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
108010076504 Protein Sorting Signals Proteins 0.000 description 1
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
208000022292 Tay-Sachs disease Diseases 0.000 description 1
230000005856 abnormality Effects 0.000 description 1
230000004075 alteration Effects 0.000 description 1
150000001412 amines Chemical class 0.000 description 1
238000013459 approach Methods 0.000 description 1
208000005980 beta thalassemia Diseases 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
239000000975 dye Substances 0.000 description 1
238000005516 engineering process Methods 0.000 description 1
150000002118 epoxides Chemical class 0.000 description 1
230000037433 frameshift Effects 0.000 description 1
230000002068 genetic effect Effects 0.000 description 1
125000001183 hydrocarbyl group Chemical group 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
108700025694 p53 Genes Proteins 0.000 description 1
KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
235000021317 phosphate Nutrition 0.000 description 1
125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
238000002360 preparation method Methods 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
125000000548 ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
238000012216 screening Methods 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
239000000377 silicon dioxide Substances 0.000 description 1
210000002268 wool Anatomy 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Physics & Mathematics (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Chemical Kinetics & Catalysis (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

SE9302152A 1993-06-22 1993-06-22 Sätt och anordning för analys av polynukleotidsekvenser SE501439C2 (sv)

Priority Applications (13)

Application Number	Priority Date	Filing Date	Title
SE9302152A SE501439C2 (sv)	1993-06-22	1993-06-22	Sätt och anordning för analys av polynukleotidsekvenser
AU72121/94A AU698553B2 (en)	1993-06-22	1994-06-22	Parallel primer extension approach to nucleic acid sequence analysis
CA002164715A CA2164715A1 (en)	1993-06-22	1994-06-22	Parallel primer extension approach to nucleic acid sequence analysis
DE69421277T DE69421277T2 (de)	1993-06-22	1994-06-22	NUKLEINSäURE-SEQUENZANALYSE DURCH DIE METHODE DER PARALLELEN PRIMEREXTENSION
ES94921363T ES2141828T3 (es)	1993-06-22	1994-06-22	Acceso a analisis de secuencias de acidos nucleicos por extension de cebadores en paralelo.
US08/564,100 US6153379A (en)	1993-06-22	1994-06-22	Parallel primer extension approach to nucleic acid sequence analysis
EP94921363A EP0705349B1 (de)	1993-06-22	1994-06-22	NUKLEINSäURE-SEQUENZANALYSE DURCH DIE METHODE DER PARALLELEN PRIMEREXTENSION
AT94921363T ATE185843T1 (de)	1993-06-22	1994-06-22	Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension
EP99200676A EP0969103A3 (de)	1993-06-22	1994-06-22	Nukleinsäure-Sequenzanalyse durch die Methode der parallelen Primerextension
PCT/US1994/007086 WO1995000669A1 (en)	1993-06-22	1994-06-22	Parallel primer extension approach to nucleic acid sequence analysis
JP50308095A JP3722833B2 (ja)	1993-06-22	1994-06-22	核酸配列分析の平行プライマー拡張手法
US09/711,476 US7001722B1 (en)	1993-06-22	2000-11-13	Parallel primer extension approach to nucleic acid sequence analysis
US10/254,828 US20030082613A1 (en)	1993-06-22	2002-09-25	Parallel primer extension approach to nucleic acid sequence analysis

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
SE9302152A SE501439C2 (sv)	1993-06-22	1993-06-22	Sätt och anordning för analys av polynukleotidsekvenser

Publications (3)

Publication Number	Publication Date
SE9302152D0 SE9302152D0 (sv)	1993-06-22
SE9302152L SE9302152L (sv)	1994-12-23
SE501439C2 true SE501439C2 (sv)	1995-02-13

Family

ID=20390371

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
SE9302152A SE501439C2 (sv)	1993-06-22	1993-06-22	Sätt och anordning för analys av polynukleotidsekvenser

Country Status (9)

Country	Link
EP (2)	EP0705349B1 (de)
JP (1)	JP3722833B2 (de)
AT (1)	ATE185843T1 (de)
AU (1)	AU698553B2 (de)
CA (1)	CA2164715A1 (de)
DE (1)	DE69421277T2 (de)
ES (1)	ES2141828T3 (de)
SE (1)	SE501439C2 (de)
WO (1)	WO1995000669A1 (de)

Families Citing this family (92)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JPH07203998A (ja) *	1994-01-26	1995-08-08	Hamamatsu Photonics Kk	核酸塩基配列決定方法
GB9507238D0 (en)	1995-04-07	1995-05-31	Isis Innovation	Detecting dna sequence variations
US5763175A (en) *	1995-11-17	1998-06-09	Lynx Therapeutics, Inc.	Simultaneous sequencing of tagged polynucleotides
WO1997035033A1 (en)	1996-03-19	1997-09-25	Molecular Tool, Inc.	Method for determining the nucleotide sequence of a polynucleotide
US6143529A (en) *	1996-08-14	2000-11-07	Exact Laboratories, Inc.	Methods for improving sensitivity and specificity of screening assays
US6096273A (en)	1996-11-05	2000-08-01	Clinical Micro Sensors	Electrodes linked via conductive oligomers to nucleic acids
US6566101B1 (en)	1997-06-16	2003-05-20	Anthony P. Shuber	Primer extension methods for detecting nucleic acids
US5888778A (en) *	1997-06-16	1999-03-30	Exact Laboratories, Inc.	High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
JP4789294B2 (ja) *	1997-08-22	2011-10-12	ソレクサ・インコーポレイテッド	ローリングプライマーを用いたｄｎａ伸長および分析
US6322968B1 (en)	1997-11-21	2001-11-27	Orchid Biosciences, Inc.	De novo or “universal” sequencing array
CA2323830A1 (en)	1998-03-16	1999-09-23	Millennium Pharmaceuticals, Inc.	Methods and compositions for diagnosing and treating chromosome-18p related disorders
US6872521B1 (en)	1998-06-16	2005-03-29	Beckman Coulter, Inc.	Polymerase signaling assay
US6245507B1 (en)	1998-08-18	2001-06-12	Orchid Biosciences, Inc.	In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
US6150105A (en) *	1998-08-20	2000-11-21	Genetic Assays, Inc.	Methods of screening nucleic acids for nucleotide variations
EP1169479B1 (de)	1999-04-09	2006-06-28	EXACT Sciences Corporation	Verfahren zur detektion von nukleinsäuren, welche auf krebs hinweisen
AU779220B2 (en)	1999-07-26	2005-01-13	Clinical Micro Sensors, Inc.	Sequence determination of nucleic acids using electronic detection
US6528319B1 (en) *	1999-09-02	2003-03-04	Amersham Biosciences Corp	Method for anchoring oligonucleotides to a substrate
US6586177B1 (en)	1999-09-08	2003-07-01	Exact Sciences Corporation	Methods for disease detection
US6849403B1 (en)	1999-09-08	2005-02-01	Exact Sciences Corporation	Apparatus and method for drug screening
EP1257662A2 (de) *	1999-10-06	2002-11-20	Amersham Biosciences Corp.	Methode zum nachweis von mutationen mittels primerverlängerung an arrays
US6596483B1 (en)	1999-11-12	2003-07-22	Motorola, Inc.	System and method for detecting molecules using an active pixel sensor
CA2394921A1 (en)	1999-12-07	2001-06-14	Anthony P. Shuber	Supracolonic aerodigestive neoplasm detection
US6919174B1 (en)	1999-12-07	2005-07-19	Exact Sciences Corporation	Methods for disease detection
DE10010282B4 (de)	2000-02-25	2006-11-16	Epigenomics Ag	Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010280B4 (de) *	2000-02-25	2006-08-10	Epigenomics Ag	Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
WO2001068913A2 (en) *	2000-03-13	2001-09-20	Genset	Nucleic acid detection method and system
US6376191B1 (en) *	2000-03-22	2002-04-23	Mergen, Ltd.	Microarray-based analysis of polynucleotide sequence variations
WO2001077375A2 (en)	2000-04-06	2001-10-18	Epigenomics Ag	Diagnosis of diseases associated with gene regulation
US6803211B2 (en)	2000-08-25	2004-10-12	Pfizer Inc.	Methods and compositions for diagnosing and treating disorders involving angiogenesis
AU2001216942A1 (en) *	2000-11-24	2002-06-03	Asper Ou	Method of optimising the sequences of synthetic nucleic acids
GB0105790D0 (en) *	2001-03-08	2001-04-25	Expresson Biosystems Ltd	Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes
WO2002079516A1 (en) *	2001-03-30	2002-10-10	Applera Corporation	Nucleic acid analysis using non-templated nucleotide addition
US6653082B2 (en)	2001-05-17	2003-11-25	Baylor College Of Medicine	Substrate-bound cleavage assay for nucleic acid analysis
EP2354156A3 (de)	2001-08-14	2011-11-16	Sentigen Biosciences, Inc.	Nukleinsäuren und Proteine von Insekten OR83B-Duftrezeptorgenen und Verwendungen davon
DE10154317B4 (de)	2001-10-26	2005-06-09	Epigenomics Ag	Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
EP1340818A1 (de)	2002-02-27	2003-09-03	Epigenomics AG	Verfahren und Nukleinsäuren zur Analyse von Kolonkrebszellen
DE10245145B4 (de) *	2002-09-27	2004-12-02	IPK-Institut für Pflanzengenetik und Kulturpflanzenforschung	Verfahren zum Nachweis von SNPs auf polydimensionalen Microarrays
EP1554407B1 (de)	2002-10-01	2009-07-29	Epigenomics AG	Verfahren für die behandlung von proliferativen erkrankungen von brustzellen
WO2005003304A2 (en)	2003-06-20	2005-01-13	Illumina, Inc.	Methods and compositions for whole genome amplification and genotyping
US8163488B2 (en)	2003-06-23	2012-04-24	Epigenomics Ag	Methods and nucleic acids for analysis of colon proliferative disorders
AU2004252554B2 (en)	2003-06-23	2012-01-19	Epigenomics Ag	Methods and nucleic acids for analyses of colorectal cell proliferative disorders
WO2005001141A2 (en)	2003-06-23	2005-01-06	Epigenomics Ag	Methods and nucleic acids for analyses of colorectal cell proliferative disorders
ES2801379T3 (es)	2003-12-01	2021-01-11	Epigenomics Ag	Métodos y ácidos nucleicos para el análisis de la expresión génica asociada con el desarrollo de trastornos proliferativos de células de próstata
CA2549852A1 (en)	2003-12-11	2005-06-30	Epigenomics Ag	Method and nucleic acids for the improved treatment of breast cell proliferative disorders
WO2006002344A1 (en)	2004-06-23	2006-01-05	Epigenomics Ag	Methods and nucleic acids for the detection of metastasis of colon cell proliferative disorders
US20090298054A1 (en)	2004-07-18	2009-12-03	Epigenomics Ag	Epigenetic methods and nucleic acids for the detection of breast cell proliferative disorders
JP5249581B2 (ja)	2004-08-09	2013-07-31	ジェネレイションバイオテックリミテッドライアビリティカンパニー	核酸の単離および増幅方法
US9109256B2 (en)	2004-10-27	2015-08-18	Esoterix Genetic Laboratories, Llc	Method for monitoring disease progression or recurrence
EP2311984A1 (de)	2004-12-02	2011-04-20	Epigenomics AG	Verfahren und Nukleinsäuren zur Analyse von mit der Prognose von Störungen der Proliferation von Prostatazellen assoziierter Genexpression
EA013634B1 (ru)	2005-04-15	2010-06-30	Эпиджиномикс Аг	Способы и нуклеиновые кислоты для анализов клеточных пролиферативных нарушений
US9777314B2 (en)	2005-04-21	2017-10-03	Esoterix Genetic Laboratories, Llc	Analysis of heterogeneous nucleic acid samples
EP2386654A1 (de)	2005-05-02	2011-11-16	University of Southern California	Mit CpG-Insel-Methylatorphänotyp (CIMP) in menschlichem Kolorektalkrebs assoziierte DNA-Methylierungsmarker
EP2298932A1 (de)	2005-09-29	2011-03-23	Epigenomics AG	Verfahren und DNS zur Analyse der Genexpression, insbesondere die Methylierung von KAAG1, für die Klassifizierung von Zellen und Gewebe
US8912129B2 (en)	2005-11-17	2014-12-16	Epigenomics Ag	Method for the determination of the DNA methylation level of a CPG position in identical cells within a tissue sample
CA2649777C (en)	2006-04-17	2018-08-14	Epigenomics Ag	Methods and nucleic acids for the detection of colorectal cell proliferative disorders
EP2041574B1 (de)	2006-07-14	2016-11-16	The Regents of The University of California	Krebsbiomarker und anwendungsverfahren dafür
EP2044221B1 (de)	2006-07-21	2015-07-22	Epigenomics AG	Verfahren und kit zur analyse der methylierung in kolorektalen proliferativen zellerkrankungen
EP3184649A1 (de)	2006-11-24	2017-06-28	Epigenomics AG	Verfahren und nukleinsäuren zur analyse der genexpression, die mit der entwicklung von prostatazellproliferationserkrankungen assoziiert ist
WO2008087040A2 (en)	2007-01-19	2008-07-24	Epigenomics Ag	Methods and nucleic acids for analyses of cell proliferative disorders
CA2687218A1 (en) *	2007-05-14	2008-11-27	Insight Genetics, Inc.	Methods of screening nucleic acids for single nucleotide variations
EP2171097A2 (de)	2007-06-29	2010-04-07	Population Genetics Technologies LTD.	Verfahren und zusammensetzungen zur isolierung der varianten von nukleinsäuresequenzen
CA2708163A1 (en)	2007-12-11	2009-06-18	Epigenomics Ag	Methods and nucleic acids for analyses of cell proliferative disorders
EP2966181B1 (de)	2009-06-26	2018-02-14	Epigenomics AG	Verfahren und nukleinsäuren zur analyse des blasenkarzinom
US20110104695A1 (en)	2009-11-05	2011-05-05	Epigenomics Ag	Methods of predicting therapeutic efficacy of cancer therapy
US12571798B2 (en)	2010-04-27	2026-03-10	The Regents Of The University Of California	Cancer biomarkers and methods of use thereof
US8916344B2 (en)	2010-11-15	2014-12-23	Exact Sciences Corporation	Methylation assay
US8361720B2 (en)	2010-11-15	2013-01-29	Exact Sciences Corporation	Real time cleavage assay
NO2729579T3 (de)	2011-07-08	2018-03-03
CN105378108A (zh)	2013-03-13	2016-03-02	雅培分子公司	用于分离核酸的系统和方法
EP2971171A4 (de)	2013-03-14	2016-11-02	Abbott Molecular Inc	Multiplex-methylierungs-spezifische verstärkersysteme und -verfahren
CA2902916C (en)	2013-03-14	2018-08-28	Mayo Foundation For Medical Education And Research	Detecting neoplasm
WO2015124921A1 (en)	2014-02-19	2015-08-27	The University Court Of The University Of Edinburgh	Methods and uses for determining the presence of inflammatory bowel disease
CN120700143A (zh)	2014-03-31	2025-09-26	梅奥医学教育和研究基金会	检测结直肠赘生物
US10184154B2 (en)	2014-09-26	2019-01-22	Mayo Foundation For Medical Education And Research	Detecting cholangiocarcinoma
WO2016090323A1 (en)	2014-12-05	2016-06-09	Prelude, Inc.	Dcis recurrence and invasive breast cancer
CA3183545C (en)	2014-12-12	2026-01-13	Exact Sciences Corporation	Compositions and methods for performing methylation detection assays
US10435755B2 (en)	2015-03-27	2019-10-08	Exact Sciences Development Company, Llc	Detecting esophageal disorders
CN114574585A (zh)	2015-08-31	2022-06-03	梅约医药教育及研究基金会	检测胃肿瘤
CN108350485A (zh)	2015-10-30	2018-07-31	精密科学发展有限责任公司	血浆dna的多重扩增检测测定以及分离和检测
CN109563546B (zh)	2016-05-05	2022-09-09	精密科学发展有限责任公司	通过分析甲基化dna来检测肺肿瘤
EP3978624B1 (de)	2016-07-19	2025-03-26	Exact Sciences Corporation	Methylierte kontroll-dna
EP3507298B1 (de)	2016-09-02	2023-08-30	Mayo Foundation for Medical Education and Research	Erkennung eines leberzellkarzinoms
KR102892245B1 (ko)	2017-01-27	2025-11-27	이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨	메틸화된 dna 분석에 의한 결장 신조직형성의 검출
JP7356349B2 (ja)	2017-02-28	2023-10-04	マヨファウンデーションフォアメディカルエデュケーションアンドリサーチ	前立腺癌検出
JP7277460B2 (ja)	2017-11-30	2023-05-19	マヨファウンデーションフォアメディカルエデュケーションアンドリサーチ	乳癌の検出
US10648025B2 (en)	2017-12-13	2020-05-12	Exact Sciences Development Company, Llc	Multiplex amplification detection assay II
US20220187301A1 (en)	2018-09-14	2022-06-16	Prelude Corporation	Method of selection for treatment of subjects at risk of invasive breast cancer
EP3880847B1 (de)	2018-11-16	2025-09-03	Oslo Universitetssykehus HF	Verfahren und zusammensetzungen zur charakterisierung von blasenkrebs
CA3127329A1 (en)	2019-01-24	2020-07-30	Mayo Foundation For Medical Education And Research	Detecting endometrial cancer
US11702704B2 (en)	2019-10-31	2023-07-18	Mayo Foundation For Medical Education And Research	Detecting ovarian cancer
AU2021329899A1 (en)	2020-08-19	2023-03-09	Exact Sciences Corporation	Detecting non-hodgkin lymphoma
KR20230150814A (ko)	2021-01-29	2023-10-31	메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치	다양한 유형의 암에 대한 존재 또는 부재의 검출

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5202231A (en) *	1987-04-01	1993-04-13	Drmanac Radoje T	Method of sequencing of genomes by hybridization of oligonucleotide probes
GB8810400D0 (en) *	1988-05-03	1988-06-08	Southern E	Analysing polynucleotide sequences
US5547839A (en) *	1989-06-07	1996-08-20	Affymax Technologies N.V.	Sequencing of surface immobilized polymers utilizing microflourescence detection
EP0834575B1 (de) *	1990-12-06	2001-11-28	Affymetrix, Inc. (a Delaware Corporation)	Identifizierung von Nukleinsäuren in Proben
US6004744A (en) *	1991-03-05	1999-12-21	Molecular Tool, Inc.	Method for determining nucleotide identity through extension of immobilized primer

1993
- 1993-06-22 SE SE9302152A patent/SE501439C2/sv not_active IP Right Cessation
1994
- 1994-06-22 DE DE69421277T patent/DE69421277T2/de not_active Expired - Fee Related
- 1994-06-22 WO PCT/US1994/007086 patent/WO1995000669A1/en not_active Ceased
- 1994-06-22 JP JP50308095A patent/JP3722833B2/ja not_active Expired - Fee Related
- 1994-06-22 EP EP94921363A patent/EP0705349B1/de not_active Expired - Lifetime
- 1994-06-22 AU AU72121/94A patent/AU698553B2/en not_active Ceased
- 1994-06-22 EP EP99200676A patent/EP0969103A3/de not_active Withdrawn
- 1994-06-22 AT AT94921363T patent/ATE185843T1/de not_active IP Right Cessation
- 1994-06-22 ES ES94921363T patent/ES2141828T3/es not_active Expired - Lifetime
- 1994-06-22 CA CA002164715A patent/CA2164715A1/en not_active Abandoned

Also Published As

Publication number	Publication date
AU698553B2 (en)	1998-10-29
SE9302152L (sv)	1994-12-23
CA2164715A1 (en)	1995-01-05
DE69421277D1 (de)	1999-11-25
SE9302152D0 (sv)	1993-06-22
ATE185843T1 (de)	1999-11-15
WO1995000669A1 (en)	1995-01-05
DE69421277T2 (de)	2000-05-31
EP0969103A2 (de)	2000-01-05
JP3722833B2 (ja)	2005-11-30
EP0705349A1 (de)	1996-04-10
JPH09501312A (ja)	1997-02-10
ES2141828T3 (es)	2000-04-01
EP0705349B1 (de)	1999-10-20
EP0969103A3 (de)	2004-01-02
AU7212194A (en)	1995-01-17

Legal Events

Date	Code	Title	Description
2000-05-21	NUG	Patent has lapsed
2008-02-19	NUG	Patent has lapsed

Publication	Publication Date	Title
SE501439C2 (sv)	1995-02-13	Sätt och anordning för analys av polynukleotidsekvenser
US6153379A (en)	2000-11-28	Parallel primer extension approach to nucleic acid sequence analysis
EP0777747B1 (de)	2001-12-05	Nukleotid-sequenzierungsmethode
EP1711631B1 (de)	2009-09-30	Nukleinsäurecharakterisierung
EP0371437B1 (de)	1996-02-21	Verfahren und Reagenzkombination zur Bestimmung von Nukleotidsequenzen
EP0907752B1 (de)	2002-06-26	Verfahren zur bestimmung von nukleinsäure-sequenzen und diagnostische anwendungen derselben
KR980700433A (ko)	1998-03-30	디엔에이(dna) 시퀀싱 및 디엔에이(dna) 동정을 위한 방법 및 장치(methods and apparatus for dna sequencing and dna identification)
IL152727A (en)	2010-02-17	Single copy genomic hybridization probes and method of generating same
KR870009024A (ko)	1987-10-22	폴리뉴클레오티드의 생산 방법
US20080090733A1 (en)	2008-04-17	Method for selectively isolating a nucleic acid
WO2004050915A1 (en)	2004-06-17	Determination of methylation of nucleic acid sequences
JP2008515451A (ja)	2008-05-15	核酸の標識化および配列決定
DE69924140T2 (de)	2006-01-19	Bestimmung der länge von repetitiven nukleinsäure-sequenzen durch eine diskontinuierliche primerverlängerung
WO2003016565A2 (en)	2003-02-27	Dna sequence analysis
WO2017204572A1 (ko)	2017-11-30	분자 바코딩을 이용한 초병렬 시퀀싱을 위한 라이브러리 제조방법 및 그의 용도
WO1999028494A1 (en)	1999-06-10	Methods of using probes for analyzing polynucleotide sequence
FI3969613T3 (fi)	2023-10-06	Välineistöt genotyyppausta varten
WO1993014224A1 (en)	1993-07-22	Size-calibration dna fragment mixture and method
US7001722B1 (en)	2006-02-21	Parallel primer extension approach to nucleic acid sequence analysis
JP3640840B2 (ja)	2005-04-20	核酸断片の分離方法及び核酸シークエンス前処理装置
AU785211B2 (en)	2006-11-02	Method for selectively isolating a nucleic acid
WO2004050916A1 (en)	2004-06-17	Recovery of original template
CN118600567A (zh)	2024-09-06	一种dna文库及其构建方法及其应用
WO2026015755A1 (en)	2026-01-15	Method for nucleic acid sequence detection and sequencing method
CN114214414A (zh)	2022-03-22	一种用于检测ALL相关基因SNP的SNaPshot多重分析试剂盒及其应用