ATE185843T1 - Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension - Google Patents

Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension

Info

Publication number
ATE185843T1
ATE185843T1 AT94921363T AT94921363T ATE185843T1 AT E185843 T1 ATE185843 T1 AT E185843T1 AT 94921363 T AT94921363 T AT 94921363T AT 94921363 T AT94921363 T AT 94921363T AT E185843 T1 ATE185843 T1 AT E185843T1
Authority
AT
Austria
Prior art keywords
polynucleotide
nucleic acid
acid sequence
sequence analysis
primer extension
Prior art date
Application number
AT94921363T
Other languages
English (en)
Inventor
C Thomas Caskey
John Shumaker
Andres Metspalu
Original Assignee
Pharmacia Biotech Ab
Baylor College Medicine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharmacia Biotech Ab, Baylor College Medicine filed Critical Pharmacia Biotech Ab
Application granted granted Critical
Publication of ATE185843T1 publication Critical patent/ATE185843T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT94921363T 1993-06-22 1994-06-22 Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension ATE185843T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9302152A SE501439C2 (sv) 1993-06-22 1993-06-22 Sätt och anordning för analys av polynukleotidsekvenser

Publications (1)

Publication Number Publication Date
ATE185843T1 true ATE185843T1 (de) 1999-11-15

Family

ID=20390371

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94921363T ATE185843T1 (de) 1993-06-22 1994-06-22 Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension

Country Status (9)

Country Link
EP (2) EP0705349B1 (de)
JP (1) JP3722833B2 (de)
AT (1) ATE185843T1 (de)
AU (1) AU698553B2 (de)
CA (1) CA2164715A1 (de)
DE (1) DE69421277T2 (de)
ES (1) ES2141828T3 (de)
SE (1) SE501439C2 (de)
WO (1) WO1995000669A1 (de)

Families Citing this family (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07203998A (ja) * 1994-01-26 1995-08-08 Hamamatsu Photonics Kk 核酸塩基配列決定方法
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5763175A (en) * 1995-11-17 1998-06-09 Lynx Therapeutics, Inc. Simultaneous sequencing of tagged polynucleotides
WO1997035033A1 (en) 1996-03-19 1997-09-25 Molecular Tool, Inc. Method for determining the nucleotide sequence of a polynucleotide
US6143529A (en) * 1996-08-14 2000-11-07 Exact Laboratories, Inc. Methods for improving sensitivity and specificity of screening assays
US6096273A (en) 1996-11-05 2000-08-01 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
US6566101B1 (en) 1997-06-16 2003-05-20 Anthony P. Shuber Primer extension methods for detecting nucleic acids
US5888778A (en) * 1997-06-16 1999-03-30 Exact Laboratories, Inc. High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers
JP4789294B2 (ja) * 1997-08-22 2011-10-12 ソレクサ・インコーポレイテッド ローリングプライマーを用いたｄｎａ伸長および分析
US6322968B1 (en) 1997-11-21 2001-11-27 Orchid Biosciences, Inc. De novo or “universal” sequencing array
CA2323830A1 (en) 1998-03-16 1999-09-23 Millennium Pharmaceuticals, Inc. Methods and compositions for diagnosing and treating chromosome-18p related disorders
US6872521B1 (en) 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
US6245507B1 (en) 1998-08-18 2001-06-12 Orchid Biosciences, Inc. In-line complete hyperspectral fluorescent imaging of nucleic acid molecules
US6150105A (en) * 1998-08-20 2000-11-21 Genetic Assays, Inc. Methods of screening nucleic acids for nucleotide variations
EP1169479B1 (de) 1999-04-09 2006-06-28 EXACT Sciences Corporation Verfahren zur detektion von nukleinsäuren, welche auf krebs hinweisen
AU779220B2 (en) 1999-07-26 2005-01-13 Clinical Micro Sensors, Inc. Sequence determination of nucleic acids using electronic detection
US6528319B1 (en) * 1999-09-02 2003-03-04 Amersham Biosciences Corp Method for anchoring oligonucleotides to a substrate
US6586177B1 (en) 1999-09-08 2003-07-01 Exact Sciences Corporation Methods for disease detection
US6849403B1 (en) 1999-09-08 2005-02-01 Exact Sciences Corporation Apparatus and method for drug screening
EP1257662A2 (de) * 1999-10-06 2002-11-20 Amersham Biosciences Corp. Methode zum nachweis von mutationen mittels primerverlängerung an arrays
US6596483B1 (en) 1999-11-12 2003-07-22 Motorola, Inc. System and method for detecting molecules using an active pixel sensor
CA2394921A1 (en) 1999-12-07 2001-06-14 Anthony P. Shuber Supracolonic aerodigestive neoplasm detection
US6919174B1 (en) 1999-12-07 2005-07-19 Exact Sciences Corporation Methods for disease detection
DE10010282B4 (de) 2000-02-25 2006-11-16 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
DE10010280B4 (de) * 2000-02-25 2006-08-10 Epigenomics Ag Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben
WO2001068913A2 (en) * 2000-03-13 2001-09-20 Genset Nucleic acid detection method and system
US6376191B1 (en) * 2000-03-22 2002-04-23 Mergen, Ltd. Microarray-based analysis of polynucleotide sequence variations
WO2001077375A2 (en) 2000-04-06 2001-10-18 Epigenomics Ag Diagnosis of diseases associated with gene regulation
US6803211B2 (en) 2000-08-25 2004-10-12 Pfizer Inc. Methods and compositions for diagnosing and treating disorders involving angiogenesis
AU2001216942A1 (en) * 2000-11-24 2002-06-03 Asper Ou Method of optimising the sequences of synthetic nucleic acids
GB0105790D0 (en) * 2001-03-08 2001-04-25 Expresson Biosystems Ltd Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes
WO2002079516A1 (en) * 2001-03-30 2002-10-10 Applera Corporation Nucleic acid analysis using non-templated nucleotide addition
US6653082B2 (en) 2001-05-17 2003-11-25 Baylor College Of Medicine Substrate-bound cleavage assay for nucleic acid analysis
EP2354156A3 (de) 2001-08-14 2011-11-16 Sentigen Biosciences, Inc. Nukleinsäuren und Proteine von Insekten OR83B-Duftrezeptorgenen und Verwendungen davon
DE10154317B4 (de) 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
EP1340818A1 (de) 2002-02-27 2003-09-03 Epigenomics AG Verfahren und Nukleinsäuren zur Analyse von Kolonkrebszellen
DE10245145B4 (de) * 2002-09-27 2004-12-02 IPK-Institut für Pflanzengenetik und Kulturpflanzenforschung Verfahren zum Nachweis von SNPs auf polydimensionalen Microarrays
EP1554407B1 (de) 2002-10-01 2009-07-29 Epigenomics AG Verfahren für die behandlung von proliferativen erkrankungen von brustzellen
WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
US8163488B2 (en) 2003-06-23 2012-04-24 Epigenomics Ag Methods and nucleic acids for analysis of colon proliferative disorders
AU2004252554B2 (en) 2003-06-23 2012-01-19 Epigenomics Ag Methods and nucleic acids for analyses of colorectal cell proliferative disorders
WO2005001141A2 (en) 2003-06-23 2005-01-06 Epigenomics Ag Methods and nucleic acids for analyses of colorectal cell proliferative disorders
ES2801379T3 (es) 2003-12-01 2021-01-11 Epigenomics Ag Métodos y ácidos nucleicos para el análisis de la expresión génica asociada con el desarrollo de trastornos proliferativos de células de próstata
CA2549852A1 (en) 2003-12-11 2005-06-30 Epigenomics Ag Method and nucleic acids for the improved treatment of breast cell proliferative disorders
WO2006002344A1 (en) 2004-06-23 2006-01-05 Epigenomics Ag Methods and nucleic acids for the detection of metastasis of colon cell proliferative disorders
US20090298054A1 (en) 2004-07-18 2009-12-03 Epigenomics Ag Epigenetic methods and nucleic acids for the detection of breast cell proliferative disorders
JP5249581B2 (ja) 2004-08-09 2013-07-31 ジェネレイション バイオテック リミテッド ライアビリティ カンパニー 核酸の単離および増幅方法
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
EP2311984A1 (de) 2004-12-02 2011-04-20 Epigenomics AG Verfahren und Nukleinsäuren zur Analyse von mit der Prognose von Störungen der Proliferation von Prostatazellen assoziierter Genexpression
EA013634B1 (ru) 2005-04-15 2010-06-30 Эпиджиномикс Аг Способы и нуклеиновые кислоты для анализов клеточных пролиферативных нарушений
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
EP2386654A1 (de) 2005-05-02 2011-11-16 University of Southern California Mit CpG-Insel-Methylatorphänotyp (CIMP) in menschlichem Kolorektalkrebs assoziierte DNA-Methylierungsmarker
EP2298932A1 (de) 2005-09-29 2011-03-23 Epigenomics AG Verfahren und DNS zur Analyse der Genexpression, insbesondere die Methylierung von KAAG1, für die Klassifizierung von Zellen und Gewebe
US8912129B2 (en) 2005-11-17 2014-12-16 Epigenomics Ag Method for the determination of the DNA methylation level of a CPG position in identical cells within a tissue sample
CA2649777C (en) 2006-04-17 2018-08-14 Epigenomics Ag Methods and nucleic acids for the detection of colorectal cell proliferative disorders
EP2041574B1 (de) 2006-07-14 2016-11-16 The Regents of The University of California Krebsbiomarker und anwendungsverfahren dafür
EP2044221B1 (de) 2006-07-21 2015-07-22 Epigenomics AG Verfahren und kit zur analyse der methylierung in kolorektalen proliferativen zellerkrankungen
EP3184649A1 (de) 2006-11-24 2017-06-28 Epigenomics AG Verfahren und nukleinsäuren zur analyse der genexpression, die mit der entwicklung von prostatazellproliferationserkrankungen assoziiert ist
WO2008087040A2 (en) 2007-01-19 2008-07-24 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
CA2687218A1 (en) * 2007-05-14 2008-11-27 Insight Genetics, Inc. Methods of screening nucleic acids for single nucleotide variations
EP2171097A2 (de) 2007-06-29 2010-04-07 Population Genetics Technologies LTD. Verfahren und zusammensetzungen zur isolierung der varianten von nukleinsäuresequenzen
CA2708163A1 (en) 2007-12-11 2009-06-18 Epigenomics Ag Methods and nucleic acids for analyses of cell proliferative disorders
EP2966181B1 (de) 2009-06-26 2018-02-14 Epigenomics AG Verfahren und nukleinsäuren zur analyse des blasenkarzinom
US20110104695A1 (en) 2009-11-05 2011-05-05 Epigenomics Ag Methods of predicting therapeutic efficacy of cancer therapy
US12571798B2 (en) 2010-04-27 2026-03-10 The Regents Of The University Of California Cancer biomarkers and methods of use thereof
US8916344B2 (en) 2010-11-15 2014-12-23 Exact Sciences Corporation Methylation assay
US8361720B2 (en) 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
NO2729579T3 (de) 2011-07-08 2018-03-03
CN105378108A (zh) 2013-03-13 2016-03-02 雅培分子公司 用于分离核酸的系统和方法
EP2971171A4 (de) 2013-03-14 2016-11-02 Abbott Molecular Inc Multiplex-methylierungs-spezifische verstärkersysteme und -verfahren
CA2902916C (en) 2013-03-14 2018-08-28 Mayo Foundation For Medical Education And Research Detecting neoplasm
WO2015124921A1 (en) 2014-02-19 2015-08-27 The University Court Of The University Of Edinburgh Methods and uses for determining the presence of inflammatory bowel disease
CN120700143A (zh) 2014-03-31 2025-09-26 梅奥医学教育和研究基金会 检测结直肠赘生物
US10184154B2 (en) 2014-09-26 2019-01-22 Mayo Foundation For Medical Education And Research Detecting cholangiocarcinoma
WO2016090323A1 (en) 2014-12-05 2016-06-09 Prelude, Inc. Dcis recurrence and invasive breast cancer
CA3183545C (en) 2014-12-12 2026-01-13 Exact Sciences Corporation Compositions and methods for performing methylation detection assays
US10435755B2 (en) 2015-03-27 2019-10-08 Exact Sciences Development Company, Llc Detecting esophageal disorders
CN114574585A (zh) 2015-08-31 2022-06-03 梅约医药教育及研究基金会 检测胃肿瘤
CN108350485A (zh) 2015-10-30 2018-07-31 精密科学发展有限责任公司 血浆dna的多重扩增检测测定以及分离和检测
CN109563546B (zh) 2016-05-05 2022-09-09 精密科学发展有限责任公司 通过分析甲基化dna来检测肺肿瘤
EP3978624B1 (de) 2016-07-19 2025-03-26 Exact Sciences Corporation Methylierte kontroll-dna
EP3507298B1 (de) 2016-09-02 2023-08-30 Mayo Foundation for Medical Education and Research Erkennung eines leberzellkarzinoms
KR102892245B1 (ko) 2017-01-27 2025-11-27 이그젝트 싸이언스 디블롭먼트 컴패니, 엘엘씨 메틸화된 dna 분석에 의한 결장 신조직형성의 검출
JP7356349B2 (ja) 2017-02-28 2023-10-04 マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ 前立腺癌検出
JP7277460B2 (ja) 2017-11-30 2023-05-19 マヨ ファウンデーション フォア メディカル エデュケーション アンド リサーチ 乳癌の検出
US10648025B2 (en) 2017-12-13 2020-05-12 Exact Sciences Development Company, Llc Multiplex amplification detection assay II
US20220187301A1 (en) 2018-09-14 2022-06-16 Prelude Corporation Method of selection for treatment of subjects at risk of invasive breast cancer
EP3880847B1 (de) 2018-11-16 2025-09-03 Oslo Universitetssykehus HF Verfahren und zusammensetzungen zur charakterisierung von blasenkrebs
CA3127329A1 (en) 2019-01-24 2020-07-30 Mayo Foundation For Medical Education And Research Detecting endometrial cancer
US11702704B2 (en) 2019-10-31 2023-07-18 Mayo Foundation For Medical Education And Research Detecting ovarian cancer
AU2021329899A1 (en) 2020-08-19 2023-03-09 Exact Sciences Corporation Detecting non-hodgkin lymphoma
KR20230150814A (ko) 2021-01-29 2023-10-31 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 다양한 유형의 암에 대한 존재 또는 부재의 검출

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5202231A (en) * 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
GB8810400D0 (en) * 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
EP0834575B1 (de) * 1990-12-06 2001-11-28 Affymetrix, Inc. (a Delaware Corporation) Identifizierung von Nukleinsäuren in Proben
US6004744A (en) * 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer

Also Published As

Publication number Publication date
AU698553B2 (en) 1998-10-29
SE9302152L (sv) 1994-12-23
CA2164715A1 (en) 1995-01-05
DE69421277D1 (de) 1999-11-25
SE9302152D0 (sv) 1993-06-22
WO1995000669A1 (en) 1995-01-05
DE69421277T2 (de) 2000-05-31
EP0969103A2 (de) 2000-01-05
JP3722833B2 (ja) 2005-11-30
EP0705349A1 (de) 1996-04-10
JPH09501312A (ja) 1997-02-10
ES2141828T3 (es) 2000-04-01
EP0705349B1 (de) 1999-10-20
SE501439C2 (sv) 1995-02-13
EP0969103A3 (de) 2004-01-02
AU7212194A (en) 1995-01-17

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