SG109552A1 - Thermostable taq polymerase fragment - Google Patents

Thermostable taq polymerase fragment

Info

Publication number
SG109552A1
SG109552A1 SG200404410A SG200404410A SG109552A1 SG 109552 A1 SG109552 A1 SG 109552A1 SG 200404410 A SG200404410 A SG 200404410A SG 200404410 A SG200404410 A SG 200404410A SG 109552 A1 SG109552 A1 SG 109552A1
Authority
SG
Singapore
Prior art keywords
delta
taq
taqwt
nucleic acid
well
Prior art date
Application number
SG200404410A
Other languages
English (en)
Inventor
Waltraud Ankenbauer Dr
Thomas Meier Dr
Annette Deufel Dr
Dieter Heindl Dr
Gisela Betzl Dr
Rainer Schmuck Dr
Bernd Schneidinger Dr
Strey Jessica
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of SG109552A1 publication Critical patent/SG109552A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
SG200404410A 2003-08-12 2004-08-11 Thermostable taq polymerase fragment SG109552A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP03017636 2003-08-12

Publications (1)

Publication Number Publication Date
SG109552A1 true SG109552A1 (en) 2005-03-30

Family

ID=34130067

Family Applications (1)

Application Number Title Priority Date Filing Date
SG200404410A SG109552A1 (en) 2003-08-12 2004-08-11 Thermostable taq polymerase fragment

Country Status (14)

Country Link
US (1) US7972830B2 (da)
EP (1) EP1507002B1 (da)
JP (1) JP3929061B2 (da)
KR (1) KR100626578B1 (da)
CN (1) CN1590543B (da)
AT (1) ATE348881T1 (da)
AU (1) AU2004203649B2 (da)
BR (1) BRPI0403221A (da)
CA (1) CA2474734C (da)
DE (1) DE602004003755T2 (da)
DK (1) DK1507002T3 (da)
ES (1) ES2278249T3 (da)
MX (1) MXPA04007721A (da)
SG (1) SG109552A1 (da)

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DE102006025154A1 (de) * 2006-05-30 2007-12-06 Universität Konstanz Mutierte DNA-Polymerase mit erhöhter Reverse Transkriptase Aktivität
EP2113574A1 (en) 2008-04-28 2009-11-04 Biotype AG Substances and methods for a DNA based profiling assay
EP2644708B1 (en) 2008-04-30 2014-12-17 Integrated Dna Technologies, Inc. RNASE-H-based assays utilizing modified RNA monomers
US20140255925A9 (en) * 2008-04-30 2014-09-11 Integrated Dna Technologies Modified rnase h enzymes and their uses
WO2010062777A2 (en) * 2008-11-03 2010-06-03 Kapabiosystems Modified type a dna polymerases
US9321999B2 (en) * 2009-11-19 2016-04-26 Solis Biodyne Oü Compositions for increasing polypeptide stability and activity, and related methods
US20110250598A1 (en) 2010-04-12 2011-10-13 Ulrike Fischer Detergent free polymerases
WO2012097318A2 (en) 2011-01-14 2012-07-19 Kap Abiosystems Modified dna polymerases for improved amplification
WO2012135053A2 (en) 2011-03-25 2012-10-04 Integrated Dna Technologies, Inc. Rnase h-based assays utilizing modified rna monomers
WO2012146260A1 (de) 2011-04-23 2012-11-01 Biolytix Ag Herstellung und verwendung von proteinen in der molekularbiologie
FI3461807T3 (fi) 2011-06-08 2023-09-07 Life Technologies Corp Uudenlaisten detergenttien suunnittelu ja kehitys pcr-järjestelmissä käyttöä varten
US9567628B2 (en) 2011-06-08 2017-02-14 Life Technologies Corporation Polymerization of nucleic acids using proteins having low isoelectric points
EP3539944A1 (en) 2013-10-25 2019-09-18 Life Technologies Corporation Novel compounds for use in pcr systems and applications thereof
EP3795681B1 (en) * 2014-12-16 2024-09-18 Life Technologies Corporation Polymerase compositions and methods of making and using same
JP6822974B2 (ja) 2015-05-29 2021-01-27 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ シトラコン酸無水物によって微生物を不活性化する方法
CN108473970B (zh) * 2015-11-27 2022-09-13 国立大学法人九州大学 Dna聚合酶变体
GB201604876D0 (en) * 2016-03-22 2016-05-04 Uni I Tromsø Norges Arktiske Uni Marine DNA polymerases
CN108265039B (zh) * 2016-12-30 2020-07-14 天津强微特生物科技有限公司 一种突变TaqDNA聚合酶及其纯化方法
WO2018183612A1 (en) * 2017-03-29 2018-10-04 Editas Medicine, Inc. Nucleic acid mutagenesis methods
WO2018209092A1 (en) * 2017-05-10 2018-11-15 Board Of Regents, The University Of Texas System Methods and devices related to amplifying nucleic acid at a variety of temperatures
US10961518B2 (en) 2018-01-19 2021-03-30 Bio-Rad Laboratories, Inc. Mutant DNA polymerases
GB201812428D0 (en) * 2018-07-31 2018-09-12 Bg Res Ltd Improved method for pathogen detection
JP2023524117A (ja) 2020-04-30 2023-06-08 スタブ ヴィーダ － インヴェスティガサォン エ セルヴィソス エム シエンシアス バイオロジカス エルディーエー コロナウイルスの疑いのある試料中の核酸配列を検出するための方法及びポータブルデバイス
JP2023532544A (ja) * 2020-07-01 2023-07-28 メディミューン，エルエルシー 生物学的製剤を精製するための洗浄剤及び方法
US11649441B2 (en) * 2020-10-07 2023-05-16 Abclonal Science, Inc. Taq DNA polymerase mutants for probe qPCR
WO2023122291A2 (en) * 2021-12-23 2023-06-29 Sherlock Biosciences, Inc. Methods for polypeptide purification
CN116024320A (zh) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 一种用于检测核酸的荧光定量pcr方法

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US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
NZ199722A (en) 1981-02-25 1985-12-13 Genentech Inc Dna transfer vector for expression of exogenous polypeptide in yeast;transformed yeast strain
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
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US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
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WO1991005753A1 (en) 1989-10-16 1991-05-02 E.I. Du Pont De Nemours And Company Process for chlorofluoropropanes
JP2709311B2 (ja) 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
AU8906091A (en) 1990-10-05 1992-04-28 Wayne M. Barnes Thermostable dna polymerase
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5436149A (en) 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5885813A (en) 1995-05-31 1999-03-23 Amersham Life Science, Inc. Thermostable DNA polymerases
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
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US6130045A (en) * 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
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GB9920194D0 (en) 1999-08-27 1999-10-27 Advanced Biotech Ltd A heat-stable thermostable DNA polymerase for use in nucleic acid amplification
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CA2409775C (en) 2001-12-03 2010-07-13 F. Hoffmann-La Roche Ag Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro

Also Published As

Publication number Publication date
CN1590543B (zh) 2010-05-12
AU2004203649A1 (en) 2005-03-03
CA2474734A1 (en) 2005-02-12
EP1507002A3 (en) 2005-07-27
CN1590543A (zh) 2005-03-09
JP2005058236A (ja) 2005-03-10
DE602004003755T2 (de) 2007-10-11
MXPA04007721A (es) 2005-07-12
HK1074458A1 (zh) 2005-11-11
KR20050025266A (ko) 2005-03-14
JP3929061B2 (ja) 2007-06-13
EP1507002A2 (en) 2005-02-16
KR100626578B1 (ko) 2006-09-25
ES2278249T3 (es) 2007-08-01
US20050037412A1 (en) 2005-02-17
DK1507002T3 (da) 2007-04-10
US7972830B2 (en) 2011-07-05
BRPI0403221A (pt) 2005-07-12
EP1507002B1 (en) 2006-12-20
AU2004203649B2 (en) 2006-01-12
DE602004003755D1 (de) 2007-02-01
CA2474734C (en) 2010-07-13
ATE348881T1 (de) 2007-01-15

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