TR200202196T2 - Homolog yeniden kombine etmeyi iyileştirme yöntemleri - Google Patents

Homolog yeniden kombine etmeyi iyileştirme yöntemleri

Info

Publication number
TR200202196T2
TR200202196T2 TR2002/02196T TR200202196T TR200202196T2 TR 200202196 T2 TR200202196 T2 TR 200202196T2 TR 2002/02196 T TR2002/02196 T TR 2002/02196T TR 200202196 T TR200202196 T TR 200202196T TR 200202196 T2 TR200202196 T2 TR 200202196T2
Authority
TR
Turkey
Prior art keywords
agent
homologous recombination
methods
alteration
antibody
Prior art date
Application number
TR2002/02196T
Other languages
English (en)
Inventor
Ivanov Evguenii
Original Assignee
Transkaryotic Therapies, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Transkaryotic Therapies, Inc. filed Critical Transkaryotic Therapies, Inc.
Publication of TR200202196T2 publication Critical patent/TR200202196T2/tr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4712—Cystic fibrosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Pulmonology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Led Devices (AREA)
  • Electrical Discharge Machining, Electrochemical Machining, And Combined Machining (AREA)

Abstract

Bu bulus, hedef bir DNA'nin seçilen bir bölgesinde, örn. bir hücrenin kromozomal DNA'sinda bir baskalasimi yükseltmek için bir yöntemle ilgilidir. Yöntem, bölgede (a) seçilmis DNA sekansi içeren çift sarmalli DNA sekansi; (b) homolog birlesmeyi destekleyen bir madde, örn. bir Rad52 proteini veya bunun fonksiyonel bir parçasi; ve (c) homolog olmayan uç eklenmesini inhibe eden madde örn. bir anti-Ku antikoru veya bir Ku-birlesme oligomer veya polimeri gibi bir madde saglamak ve baskalasimin gerçeklesmesini mümkün kilmaktan meydana gelir. Homolog olmayan uç eklenmesini inhibe eden madde, örn. bir anti-Ku antikoru gibi Ku'yu etkisizlestiren bir madde tercihen lokal olarak verilir. (a), (b) ve (c) maddeleri tercih edildigi gibi birlikte veya ayri ayri verilebilir.
TR2002/02196T 2000-03-14 2001-03-13 Homolog yeniden kombine etmeyi iyileştirme yöntemleri TR200202196T2 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/525,160 US6569681B1 (en) 2000-03-14 2000-03-14 Methods of improving homologous recombination

Publications (1)

Publication Number Publication Date
TR200202196T2 true TR200202196T2 (tr) 2003-01-21

Family

ID=24092175

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2002/02196T TR200202196T2 (tr) 2000-03-14 2001-03-13 Homolog yeniden kombine etmeyi iyileştirme yöntemleri

Country Status (10)

Country Link
US (1) US6569681B1 (tr)
EP (1) EP1263972B1 (tr)
JP (1) JP2003526376A (tr)
AT (1) ATE525472T1 (tr)
AU (1) AU2001245637A1 (tr)
CA (1) CA2403113A1 (tr)
DK (1) DK1263972T3 (tr)
MX (1) MXPA02009044A (tr)
TR (1) TR200202196T2 (tr)
WO (1) WO2001068882A2 (tr)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ524366A (en) * 2000-07-27 2005-12-23 Univ Delaware Methods for enhancing targeted gene alteration using oligonucleotides
DK1395669T3 (da) * 2001-01-26 2009-11-16 Selexis Sa Matriks bindingsregioner og fremgangsmåder til anvendelse af disse
CA2473741C (en) * 2002-01-18 2015-12-22 Morphotek, Inc. A method for generating engineered cells for locus specific gene regulation and analysis
GB0214896D0 (en) * 2002-06-27 2002-08-07 Novartis Forschungsstiftung Gene for increased somatic recombination
FR2846337B1 (fr) * 2002-10-28 2007-06-22 Centre Nat Rech Scient Procede pour inserer un acide nucleique d'interet dans une cellule procaryote ou eucaryote par recombinaison homologue
WO2004061077A2 (en) * 2002-12-24 2004-07-22 North Carolina State University Increasing gamete production with a gene switch
KR101269656B1 (ko) 2003-10-24 2013-05-30 셀렉시스 에스. 에이. Ｍａｒ 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현
JP4050712B2 (ja) 2004-02-27 2008-02-20 独立行政法人科学技術振興機構 相同組換えを行わせる方法
EP3351637B1 (en) * 2004-04-02 2020-06-10 DSM IP Assets B.V. Filamentous fungal mutants with improved homologous recombination efficiency
US20060110390A1 (en) * 2004-08-25 2006-05-25 Myogen, Inc. Inhibition of Ku as a treatment for cardiovascular diseases
JP4625318B2 (ja) * 2004-12-06 2011-02-02 財団法人野田産業科学研究所 相同組換え頻度が上昇した形質転換菌
EP2166100B1 (en) 2005-03-08 2012-07-18 BASF Plant Science GmbH Expression enhancing intron sequences
EP1871868A2 (en) 2005-04-15 2008-01-02 North Carolina State University Methods and compositions to modulate adhesion and stress tolerance in bacteria
JP2007222055A (ja) * 2006-02-22 2007-09-06 Noda Inst For Scient Res 染色体領域欠失株の作製方法
JP2008263927A (ja) * 2007-04-25 2008-11-06 Noda Inst For Scient Res 染色体大領域の欠失方法
JP5497632B2 (ja) * 2007-05-09 2014-05-21 ノボザイムス アクティーゼルスカブ 関心対象のポリペプチドを産生するライブラリークローンの選択に適した発現クローニング法
NZ599338A (en) 2007-06-27 2013-11-29 Marinepolymer Tech Inc Complexes of il-15 and il-15ralpha and uses thereof
WO2011017315A2 (en) * 2009-08-03 2011-02-10 Recombinetics, Inc. Methods and compositions for targeted gene modification
CA2769822C (en) 2009-08-13 2019-02-19 The Johns Hopkins University Methods of modulating immune function
CN102791865B (zh) * 2009-12-21 2015-07-01 凯津公司 用于转染原生质体的改进技术
WO2011078662A1 (en) * 2009-12-21 2011-06-30 Keygene N.V. Dsrna for improved genetic modification of plant dna
GB201009732D0 (en) 2010-06-10 2010-07-21 Gene Bridges Gmbh Direct cloning
WO2012168307A2 (en) * 2011-06-07 2012-12-13 Helmholtz Zentrum München - Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH) Improved recombination efficiency by inhibition of nhej dna repair
EP2814969A4 (en) * 2012-02-10 2016-02-17 Univ Minnesota Dna assimilation
US20150359853A1 (en) 2012-10-24 2015-12-17 Admune Therapeutics Llc Il-15r alpha forms, cells expressing il-15r alpha forms, and therapeutic uses of il-15r alpha and il-15/il-15r alpha complexes
WO2015066205A1 (en) * 2013-10-29 2015-05-07 Rutgers, The State University Of New Jersey Fast genetically modified organism and systems for precise genome editing and targeted genetic manipulation through enhanced homologous recombination
ES2811974T3 (es) 2014-07-29 2021-03-15 Novartis Ag Regímenes de aumento escalonado de la dosis del heterodímero de il-15 e il-15ralfa para tratar afecciones
EP3200816A1 (en) 2014-09-29 2017-08-09 Alexion Pharmaceuticals, Inc. Methods of treating mucopolysaccharidosis iiib (mpsiiib)
ES2847252T3 (es) 2016-02-22 2021-08-02 Caribou Biosciences Inc Procedimientos de modulación de resultados de reparación de ADN
WO2018134784A1 (en) 2017-01-20 2018-07-26 Novartis Ag Combination therapy for the treatment of cancer
WO2018134782A1 (en) 2017-01-20 2018-07-26 Novartis Ag Combination therapy for the treatment of cancer
JP7084021B2 (ja) * 2018-01-24 2022-06-14 公立大学法人大阪 ターゲティングベクター
US20210244821A1 (en) 2020-02-05 2021-08-12 Novartis Ag Cho cell expressed het il-15

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5272071A (en) 1989-12-22 1993-12-21 Applied Research Systems Ars Holding N.V. Method for the modification of the expression characteristics of an endogenous gene of a given cell line
US6010908A (en) 1992-08-21 2000-01-04 The Regents Of The University Of California Gene therapy by small fragment homologous replacement
US5641670A (en) 1991-11-05 1997-06-24 Transkaryotic Therapies, Inc. Protein production and protein delivery
AU4115693A (en) * 1992-04-24 1993-11-29 Sri International In vivo homologous sequence targeting in eukaryotic cells
US5780296A (en) 1995-01-17 1998-07-14 Thomas Jefferson University Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
CA2277481A1 (en) 1997-01-13 1998-07-16 Kudos Pharmaceuticals Limited Assays, agents, therapy and diagnosis relating to modulation of cellular dna repair activity
GB9700574D0 (en) 1997-01-13 1997-03-05 Cancer Res Campaign Tech Methods and means relating to retrotransposon and retroviral integration
KR100570935B1 (ko) * 1997-01-17 2006-04-13 맥시겐, 인크. 반복적 서열 재조합에 의한 전세포 및 유기체의 개량 방법
US5986055A (en) 1997-11-13 1999-11-16 Curagen Corporation CDK2 interactions
DE19755952C1 (de) 1997-12-16 1998-12-10 Curt Niebling Dünnwandiges, elektro-magnetisch abschirmendes, tiefgezogenes Formteil, Verfahren zu dessen Herstellung, und ein bei diesem Verfahren einsetzbares Zwischenprodukt
US6441158B1 (en) 1997-12-31 2002-08-27 Medical College Of Georgia Research Institute, Inc. Oligomers that bind to ku protein
US6180850B1 (en) 1998-09-02 2001-01-30 Pioneer Hi-Bred International, Inc. Maize Ku70 orthologue and uses thereof
AU771438C (en) 1999-05-05 2004-12-02 Pioneer Hi-Bred International, Inc. Maize Rad50 orthologue and uses thereof

Also Published As

Publication number Publication date
US6569681B1 (en) 2003-05-27
EP1263972A2 (en) 2002-12-11
EP1263972B1 (en) 2011-09-21
MXPA02009044A (es) 2003-04-25
AU2001245637A1 (en) 2001-09-24
ATE525472T1 (de) 2011-10-15
DK1263972T3 (da) 2012-01-02
CA2403113A1 (en) 2001-09-20
WO2001068882A3 (en) 2002-03-14
JP2003526376A (ja) 2003-09-09
WO2001068882A2 (en) 2001-09-20

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