TW294722B - - Google Patents

Download PDF

Info

Publication number: TW294722B
Authority: TW; Taiwan
Prior art keywords: seq; fragment; nucleic acid; oligonucleotide; homologous
Prior art date: 1991-12-23

Application number

TW082100463A

Other languages

English (en)

Chinese (zh)

Original Assignee

Chiron Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-12-23

Filing date

1993-01-20

Publication date

1997-01-01

1993-01-20 Application filed by Chiron Corp filed Critical Chiron Corp

1997-01-01 Application granted granted Critical

1997-01-01 Publication of TW294722B publication Critical patent/TW294722B/zh

Links

239000000523 sample Substances 0.000 claims abstract description 186
238000009396 hybridization Methods 0.000 claims abstract description 66
238000001514 detection method Methods 0.000 claims abstract description 7
108091034117 Oligonucleotide Proteins 0.000 claims description 132
150000007523 nucleic acids Chemical group 0.000 claims description 119
102000039446 nucleic acids Human genes 0.000 claims description 114
108020004707 nucleic acids Proteins 0.000 claims description 114
239000012634 fragment Substances 0.000 claims description 98
239000002773 nucleotide Substances 0.000 claims description 56
125000003729 nucleotide group Chemical group 0.000 claims description 56
238000004458 analytical method Methods 0.000 claims description 55
230000000295 complement effect Effects 0.000 claims description 51
239000007790 solid phase Substances 0.000 claims description 38
239000012491 analyte Substances 0.000 claims description 31
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 29
238000011049 filling Methods 0.000 claims description 28
230000027455 binding Effects 0.000 claims description 26
AWGBKZRMLNVLAF-UHFFFAOYSA-N 3,5-dibromo-n,2-dihydroxybenzamide Chemical compound ONC(=O)C1=CC(Br)=CC(Br)=C1O AWGBKZRMLNVLAF-UHFFFAOYSA-N 0.000 claims description 24
238000000034 method Methods 0.000 claims description 23
108091033319 polynucleotide Proteins 0.000 claims description 21
102000040430 polynucleotide Human genes 0.000 claims description 21
239000002157 polynucleotide Substances 0.000 claims description 19
239000002253 acid Substances 0.000 claims description 16
239000012071 phase Substances 0.000 claims description 16
-1 H IV nucleic acid Chemical class 0.000 claims description 15
230000002079 cooperative effect Effects 0.000 claims description 9
239000000126 substance Substances 0.000 claims description 9
238000003786 synthesis reaction Methods 0.000 claims description 7
230000015572 biosynthetic process Effects 0.000 claims description 6
239000003550 marker Substances 0.000 claims description 6
108020005187 Oligonucleotide Probes Proteins 0.000 claims description 5
239000002777 nucleoside Substances 0.000 claims description 5
239000002751 oligonucleotide probe Substances 0.000 claims description 5
239000002131 composite material Substances 0.000 claims description 4
150000008134 glucuronides Chemical class 0.000 claims description 4
150000003833 nucleoside derivatives Chemical class 0.000 claims description 4
241000283068 Tapiridae Species 0.000 claims description 3
235000004263 Ocotea pretiosa Nutrition 0.000 claims description 2
229930182480 glucuronide Natural products 0.000 claims description 2
125000006850 spacer group Chemical group 0.000 claims 2
PCTMTFRHKVHKIS-BMFZQQSSSA-N (1s,3r,4e,6e,8e,10e,12e,14e,16e,18s,19r,20r,21s,25r,27r,30r,31r,33s,35r,37s,38r)-3-[(2r,3s,4s,5s,6r)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl]oxy-19,25,27,30,31,33,35,37-octahydroxy-18,20,21-trimethyl-23-oxo-22,39-dioxabicyclo[33.3.1]nonatriaconta-4,6,8,10 Chemical compound C1C=C2C[C@@H](OS(O)(=O)=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2.O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 PCTMTFRHKVHKIS-BMFZQQSSSA-N 0.000 claims 1
XIYOPDCBBDCGOE-IWVLMIASSA-N (4s,4ar,5s,5ar,12ar)-4-(dimethylamino)-1,5,10,11,12a-pentahydroxy-6-methylidene-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide Chemical compound C=C1C2=CC=CC(O)=C2C(O)=C2[C@@H]1[C@H](O)[C@H]1[C@H](N(C)C)C(=O)C(C(N)=O)=C(O)[C@@]1(O)C2=O XIYOPDCBBDCGOE-IWVLMIASSA-N 0.000 claims 1
244000227633 Ocotea pretiosa Species 0.000 claims 1
239000007791 liquid phase Substances 0.000 claims 1
208000011580 syndromic disease Diseases 0.000 claims 1
238000007899 nucleic acid hybridization Methods 0.000 abstract description 6
238000003556 assay Methods 0.000 abstract description 3
108020003215 DNA Probes Proteins 0.000 abstract description 2
239000003298 DNA probe Substances 0.000 abstract description 2
241000725303 Human immunodeficiency virus Species 0.000 description 84
239000000243 solution Substances 0.000 description 27
OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 26
108020004414 DNA Proteins 0.000 description 19
239000000203 mixture Substances 0.000 description 14
108091032973 (ribonucleotides)n+m Proteins 0.000 description 12
239000003153 chemical reaction reagent Substances 0.000 description 10
239000000047 product Substances 0.000 description 9
108010067770 Endopeptidase K Proteins 0.000 description 8
102000004190 Enzymes Human genes 0.000 description 8
108090000790 Enzymes Proteins 0.000 description 8
ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 7
239000007788 liquid Substances 0.000 description 7
YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 6
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
238000009434 installation Methods 0.000 description 6
238000002372 labelling Methods 0.000 description 6
238000002360 preparation method Methods 0.000 description 6
239000001509 sodium citrate Substances 0.000 description 6
NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 6
108700004026 gag Genes Proteins 0.000 description 5
230000003993 interaction Effects 0.000 description 5
239000000178 monomer Substances 0.000 description 5
108700004029 pol Genes Proteins 0.000 description 5
230000035945 sensitivity Effects 0.000 description 5
210000002966 serum Anatomy 0.000 description 5
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 4
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 4
IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 4
239000000654 additive Substances 0.000 description 4
239000007853 buffer solution Substances 0.000 description 4
239000005289 controlled pore glass Substances 0.000 description 4
239000003085 diluting agent Substances 0.000 description 4
239000001488 sodium phosphate Substances 0.000 description 4
229910000162 sodium phosphate Inorganic materials 0.000 description 4
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 4
238000005406 washing Methods 0.000 description 4
239000002699 waste material Substances 0.000 description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
108020004711 Nucleic Acid Probes Proteins 0.000 description 3
YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
150000007513 acids Chemical class 0.000 description 3
239000008186 active pharmaceutical agent Substances 0.000 description 3
239000012736 aqueous medium Substances 0.000 description 3
238000006243 chemical reaction Methods 0.000 description 3
150000001875 compounds Chemical class 0.000 description 3
230000008878 coupling Effects 0.000 description 3
238000010168 coupling process Methods 0.000 description 3
238000005859 coupling reaction Methods 0.000 description 3
239000002853 nucleic acid probe Substances 0.000 description 3
101150088264 pol gene Proteins 0.000 description 3
230000002441 reversible effect Effects 0.000 description 3
239000007787 solid Substances 0.000 description 3
239000002904 solvent Substances 0.000 description 3
239000000758 substrate Substances 0.000 description 3
KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
241000972773 Aulopiformes Species 0.000 description 2
DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
102000003960 Ligases Human genes 0.000 description 2
108090000364 Ligases Proteins 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
241000588652 Neisseria gonorrhoeae Species 0.000 description 2
108091028043 Nucleic acid sequence Proteins 0.000 description 2
JZTPOMIFAFKKSK-UHFFFAOYSA-N O-phosphonohydroxylamine Chemical compound NOP(O)(O)=O JZTPOMIFAFKKSK-UHFFFAOYSA-N 0.000 description 2
238000012408 PCR amplification Methods 0.000 description 2
229910019142 PO4 Inorganic materials 0.000 description 2
108091005804 Peptidases Proteins 0.000 description 2
239000002202 Polyethylene glycol Substances 0.000 description 2
239000004743 Polypropylene Substances 0.000 description 2
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
241000700605 Viruses Species 0.000 description 2
229960000583 acetic acid Drugs 0.000 description 2
230000000996 additive effect Effects 0.000 description 2
150000001408 amides Chemical class 0.000 description 2
150000001412 amines Chemical class 0.000 description 2
239000011324 bead Substances 0.000 description 2
IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
NEHMKBQYUWJMIP-UHFFFAOYSA-N chloromethane Chemical compound ClC NEHMKBQYUWJMIP-UHFFFAOYSA-N 0.000 description 2
WDECIBYCCFPHNR-UHFFFAOYSA-N chrysene Chemical compound C1=CC=CC2=CC=C3C4=CC=CC=C4C=CC3=C21 WDECIBYCCFPHNR-UHFFFAOYSA-N 0.000 description 2
238000010790 dilution Methods 0.000 description 2
239000012895 dilution Substances 0.000 description 2
230000000694 effects Effects 0.000 description 2
238000005516 engineering process Methods 0.000 description 2
101150098622 gag gene Proteins 0.000 description 2
239000012362 glacial acetic acid Substances 0.000 description 2
229930182470 glycoside Natural products 0.000 description 2
150000002338 glycosides Chemical class 0.000 description 2
239000000463 material Substances 0.000 description 2
239000002609 medium Substances 0.000 description 2
229910021645 metal ion Inorganic materials 0.000 description 2
239000013642 negative control Substances 0.000 description 2
YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
239000010452 phosphate Substances 0.000 description 2
239000002985 plastic film Substances 0.000 description 2
229920006255 plastic film Polymers 0.000 description 2
229920001223 polyethylene glycol Polymers 0.000 description 2
238000003752 polymerase chain reaction Methods 0.000 description 2
229920001155 polypropylene Polymers 0.000 description 2
210000002729 polyribosome Anatomy 0.000 description 2
108090000765 processed proteins & peptides Proteins 0.000 description 2
125000006239 protecting group Chemical group 0.000 description 2
108090000623 proteins and genes Proteins 0.000 description 2
UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 2
235000019515 salmon Nutrition 0.000 description 2
239000011734 sodium Substances 0.000 description 2
ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
238000003756 stirring Methods 0.000 description 2
229940104230 thymidine Drugs 0.000 description 2
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
NWZSZGALRFJKBT-KNIFDHDWSA-N (2s)-2,6-diaminohexanoic acid;(2s)-2-hydroxybutanedioic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O.NCCCC[C@H](N)C(O)=O NWZSZGALRFJKBT-KNIFDHDWSA-N 0.000 description 1
DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 description 1
TVZRAEYQIKYCPH-UHFFFAOYSA-N 3-(trimethylsilyl)propane-1-sulfonic acid Chemical compound C[Si](C)(C)CCCS(O)(=O)=O TVZRAEYQIKYCPH-UHFFFAOYSA-N 0.000 description 1
VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
108091093088 Amplicon Proteins 0.000 description 1
ZZXCYZLOYOCXLL-UHFFFAOYSA-N C(C)C(COP(ON(C(C)C)C(C)C)(O)=O)C#N Chemical compound C(C)C(COP(ON(C(C)C)C(C)C)(O)=O)C#N ZZXCYZLOYOCXLL-UHFFFAOYSA-N 0.000 description 1
IUFMDFQLINQLES-UHFFFAOYSA-N C(CCC(=O)O)(=O)O.C1(CCC(N1)=O)=O.C1(CCC(N1)=O)=O Chemical compound C(CCC(=O)O)(=O)O.C1(CCC(N1)=O)=O.C1(CCC(N1)=O)=O IUFMDFQLINQLES-UHFFFAOYSA-N 0.000 description 1
101100292356 Caenorhabditis elegans mtl-2 gene Proteins 0.000 description 1
102000014914 Carrier Proteins Human genes 0.000 description 1
108010078791 Carrier Proteins Proteins 0.000 description 1
240000004528 Catalpa ovata Species 0.000 description 1
235000010005 Catalpa ovata Nutrition 0.000 description 1
241000218645 Cedrus Species 0.000 description 1
241000606153 Chlamydia trachomatis Species 0.000 description 1
235000007516 Chrysanthemum Nutrition 0.000 description 1
244000189548 Chrysanthemum x morifolium Species 0.000 description 1
108091026890 Coding region Proteins 0.000 description 1
108091035707 Consensus sequence Proteins 0.000 description 1
206010011224 Cough Diseases 0.000 description 1
206010011703 Cyanosis Diseases 0.000 description 1
IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
101150054335 DNA-R gene Proteins 0.000 description 1
CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
238000009007 Diagnostic Kit Methods 0.000 description 1
102000010911 Enzyme Precursors Human genes 0.000 description 1
108010062466 Enzyme Precursors Proteins 0.000 description 1
229920001917 Ficoll Polymers 0.000 description 1
BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
108091027305 Heteroduplex Proteins 0.000 description 1
101000973997 Homo sapiens Nucleosome assembly protein 1-like 4 Proteins 0.000 description 1
101000947178 Homo sapiens Platelet basic protein Proteins 0.000 description 1
241000598436 Human T-cell lymphotropic virus Species 0.000 description 1
244000025221 Humulus lupulus Species 0.000 description 1
235000008694 Humulus lupulus Nutrition 0.000 description 1
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
ACFIXJIJDZMPPO-NNYOXOHSSA-N NADPH Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](OP(O)(O)=O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 ACFIXJIJDZMPPO-NNYOXOHSSA-N 0.000 description 1
102100022396 Nucleosome assembly protein 1-like 4 Human genes 0.000 description 1
244000131316 Panax pseudoginseng Species 0.000 description 1
235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
235000003140 Panax quinquefolius Nutrition 0.000 description 1
229930182555 Penicillin Natural products 0.000 description 1
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
102000035195 Peptidases Human genes 0.000 description 1
102000003992 Peroxidases Human genes 0.000 description 1
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
108010004729 Phycoerythrin Proteins 0.000 description 1
108010021757 Polynucleotide 5'-Hydroxyl-Kinase Proteins 0.000 description 1
102000008422 Polynucleotide 5'-hydroxyl-kinase Human genes 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
239000004365 Protease Substances 0.000 description 1
229920001131 Pulp (paper) Polymers 0.000 description 1
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
108091028664 Ribonucleotide Proteins 0.000 description 1
244000009660 Sassafras variifolium Species 0.000 description 1
PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
101710137500 T7 RNA polymerase Proteins 0.000 description 1
239000004098 Tetracycline Substances 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
108020005202 Viral DNA Proteins 0.000 description 1
125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 1
230000009471 action Effects 0.000 description 1
230000002776 aggregation Effects 0.000 description 1
238000004220 aggregation Methods 0.000 description 1
150000001298 alcohols Chemical class 0.000 description 1
102000005840 alpha-Galactosidase Human genes 0.000 description 1
108010030291 alpha-Galactosidase Proteins 0.000 description 1
239000000908 ammonium hydroxide Substances 0.000 description 1
230000003321 amplification Effects 0.000 description 1
230000001745 anti-biotin effect Effects 0.000 description 1
230000003460 anti-nuclear Effects 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
239000003125 aqueous solvent Substances 0.000 description 1
230000009286 beneficial effect Effects 0.000 description 1
239000013060 biological fluid Substances 0.000 description 1
230000000903 blocking effect Effects 0.000 description 1
239000000872 buffer Substances 0.000 description 1
239000004202 carbamide Substances 0.000 description 1
230000001413 cellular effect Effects 0.000 description 1
230000003196 chaotropic effect Effects 0.000 description 1
238000002144 chemical decomposition reaction Methods 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
239000005081 chemiluminescent agent Substances 0.000 description 1
229940038705 chlamydia trachomatis Drugs 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
210000001520 comb Anatomy 0.000 description 1
230000002860 competitive effect Effects 0.000 description 1
238000001816 cooling Methods 0.000 description 1
229940104302 cytosine Drugs 0.000 description 1
238000004925 denaturation Methods 0.000 description 1
230000036425 denaturation Effects 0.000 description 1
238000010511 deprotection reaction Methods 0.000 description 1
239000003599 detergent Substances 0.000 description 1
238000002405 diagnostic procedure Methods 0.000 description 1
FFYPMLJYZAEMQB-UHFFFAOYSA-N diethyl pyrocarbonate Chemical compound CCOC(=O)OC(=O)OCC FFYPMLJYZAEMQB-UHFFFAOYSA-N 0.000 description 1
238000007865 diluting Methods 0.000 description 1
125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 description 1
238000010494 dissociation reaction Methods 0.000 description 1
230000005593 dissociations Effects 0.000 description 1
238000004090 dissolution Methods 0.000 description 1
238000001035 drying Methods 0.000 description 1
239000000975 dye Substances 0.000 description 1
238000001962 electrophoresis Methods 0.000 description 1
230000002708 enhancing effect Effects 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
239000002532 enzyme inhibitor Substances 0.000 description 1
150000002148 esters Chemical class 0.000 description 1
125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
239000012530 fluid Substances 0.000 description 1
239000007850 fluorescent dye Substances 0.000 description 1
235000011389 fruit/vegetable juice Nutrition 0.000 description 1
125000000524 functional group Chemical group 0.000 description 1
235000008434 ginseng Nutrition 0.000 description 1
208000002672 hepatitis B Diseases 0.000 description 1
IKDUDTNKRLTJSI-UHFFFAOYSA-N hydrazine monohydrate Substances O.NN IKDUDTNKRLTJSI-UHFFFAOYSA-N 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
238000011534 incubation Methods 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
238000011068 loading method Methods 0.000 description 1
HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
MGJXBDMLVWIYOQ-UHFFFAOYSA-N methylazanide Chemical compound [NH-]C MGJXBDMLVWIYOQ-UHFFFAOYSA-N 0.000 description 1
238000005497 microtitration Methods 0.000 description 1
239000011259 mixed solution Substances 0.000 description 1
238000002156 mixing Methods 0.000 description 1
201000006417 multiple sclerosis Diseases 0.000 description 1
VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 1
238000006386 neutralization reaction Methods 0.000 description 1
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 description 1
230000009871 nonspecific binding Effects 0.000 description 1
VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 description 1
238000003199 nucleic acid amplification method Methods 0.000 description 1
238000002515 oligonucleotide synthesis Methods 0.000 description 1
230000010355 oscillation Effects 0.000 description 1
230000001590 oxidative effect Effects 0.000 description 1
238000004806 packaging method and process Methods 0.000 description 1
101150077062 pal gene Proteins 0.000 description 1
239000002245 particle Substances 0.000 description 1
244000052769 pathogen Species 0.000 description 1
229940049954 penicillin Drugs 0.000 description 1
KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
108040007629 peroxidase activity proteins Proteins 0.000 description 1
230000002688 persistence Effects 0.000 description 1
125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
150000003014 phosphoric acid esters Chemical class 0.000 description 1
210000002706 plastid Anatomy 0.000 description 1
229920001184 polypeptide Polymers 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
239000001267 polyvinylpyrrolidone Substances 0.000 description 1
229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
239000011148 porous material Substances 0.000 description 1
239000013641 positive control Substances 0.000 description 1
230000035935 pregnancy Effects 0.000 description 1
238000004321 preservation Methods 0.000 description 1
230000008569 process Effects 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
235000019833 protease Nutrition 0.000 description 1
235000019419 proteases Nutrition 0.000 description 1
238000000746 purification Methods 0.000 description 1
238000004445 quantitative analysis Methods 0.000 description 1
230000002285 radioactive effect Effects 0.000 description 1
BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 1
108091008146 restriction endonucleases Proteins 0.000 description 1
PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
239000002336 ribonucleotide Substances 0.000 description 1
125000002652 ribonucleotide group Chemical group 0.000 description 1
238000000926 separation method Methods 0.000 description 1
238000013207 serial dilution Methods 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
229910052938 sodium sulfate Inorganic materials 0.000 description 1
235000011152 sodium sulphate Nutrition 0.000 description 1
239000002689 soil Substances 0.000 description 1
241000894007 species Species 0.000 description 1
229940063673 spermidine Drugs 0.000 description 1
229920001909 styrene-acrylic polymer Polymers 0.000 description 1
239000013589 supplement Substances 0.000 description 1
229960002180 tetracycline Drugs 0.000 description 1
229930101283 tetracycline Natural products 0.000 description 1
235000019364 tetracycline Nutrition 0.000 description 1
150000003522 tetracyclines Chemical class 0.000 description 1
MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
210000001519 tissue Anatomy 0.000 description 1
238000004448 titration Methods 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
125000002221 trityl group Chemical group [H]C1=C([H])C([H])=C([H])C([H])=C1C([*])(C1=C(C(=C(C(=C1[H])[H])[H])[H])[H])C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 description 1
230000003612 virological effect Effects 0.000 description 1
239000011534 wash buffer Substances 0.000 description 1
239000002023 wood Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/702—Specific hybridization probes for retroviruses
- C12Q1/703—Viruses associated with AIDS
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/682—Signal amplification
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Immunology (AREA)
Virology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
General Health & Medical Sciences (AREA)
AIDS & HIV (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)
Treatment Of Water By Ion Exchange (AREA)

TW082100463A 1991-12-23 1993-01-20 TW294722B (fr)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US81358391A	1991-12-23	1991-12-23

Publications (1)

Publication Number	Publication Date
TW294722B true TW294722B (fr)	1997-01-01

Family

ID=25212818

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
TW082100463A TW294722B (fr)	1991-12-23	1993-01-20

Country Status (11)

Country	Link
US (1)	US6300056B1 (fr)
EP (2)	EP1752545A3 (fr)
JP (5)	JP3907201B2 (fr)
AT (1)	ATE329054T1 (fr)
CA (1)	CA2124797C (fr)
DE (1)	DE69233630T2 (fr)
DK (1)	DK0726962T3 (fr)
ES (1)	ES2267094T3 (fr)
PT (1)	PT726962E (fr)
TW (1)	TW294722B (fr)
WO (1)	WO1993013223A1 (fr)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6589734B1 (en)	1989-07-11	2003-07-08	Gen-Probe Incorporated	Detection of HIV
US5856088A (en) *	1989-07-11	1999-01-05	Gen-Probe Incorporated	Detection of human immunodeficiency virus type 1
KR100325554B1 (ko)	1993-03-26	2002-11-02	젠-프로브 인코포레이티드	사람의면역결핍 바이러스타입1의검출
US5681697A (en) *	1993-12-08	1997-10-28	Chiron Corporation	Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
EP0717782A1 (fr) *	1994-06-22	1996-06-26	Mount Sinai School Of Medicine Of The City University Of New York	Amplification dependant de la liaison s'appliquant a la detection d'agents pathogenes infectieux et de genes anormaux
US6593086B2 (en)	1996-05-20	2003-07-15	Mount Sinai School Of Medicine Of New York University	Nucleic acid amplification methods
US5876924A (en) *	1994-06-22	1999-03-02	Mount Sinai School Of Medicine	Nucleic acid amplification method hybridization signal amplification method (HSAM)
US5942391A (en) *	1994-06-22	1999-08-24	Mount Sinai School Of Medicine	Nucleic acid amplification method: ramification-extension amplification method (RAM)
USRE38442E1 (en) *	1994-06-22	2004-02-24	Mount Sinai School Of Medicine	Nucleic acid amplification method hybridization signal amplification method (HSAM)
US5733781A (en) *	1994-07-19	1998-03-31	Gen-Probe Incorporated	Oligonucleotides and methods for inhibiting propagation of human immunodeficiency virus
US5599662A (en) *	1995-02-17	1997-02-04	Hoffmann-La Roche Inc.	Oliconucleotide primers and probes for the detection of HIV-1
US5853974A (en) *	1995-06-07	1998-12-29	Chiron Corporation	Enhancement of alkaline phosphatase with SDS in chemiluminescent substrates
US5780227A (en) *	1995-06-07	1998-07-14	Sheridan; Patrick J.	Oligonucleotide probe conjugated to a purified hydrophilic alkaline phosphatase and uses thereof
US6265152B1 (en)	1995-12-22	2001-07-24	Visible Genetics Inc.	Method and kit for evaluation of HIV mutations
US6830887B2 (en)	1997-03-18	2004-12-14	Bayer Healthcare Llc	Method and kit for quantitation and nucleic acid sequencing of nucleic acid analytes in a sample
US5962665A (en) *	1997-06-16	1999-10-05	Abbott Laboratories	Nucleic acid primers and probes for detecting HIV-1 and HIV-2
US6261781B1 (en)	1997-08-05	2001-07-17	Wisconsin Alumni Research Foundation	Direct detection and mutation analysis of low copy number nucleic acids
US6013442A (en) *	1997-08-05	2000-01-11	Wisconsin Alumni Res Found	Direct quantitation of low copy number RNA
EP1017856A1 (fr) *	1997-09-26	2000-07-12	Visible Genetics Inc.	Procede et ensemble de materiel d'evaluation de mutations vih
US6365346B1 (en) *	1998-02-18	2002-04-02	Dade Behring Inc.	Quantitative determination of nucleic acid amplification products
WO2000046403A2 (fr)	1999-02-03	2000-08-10	THE GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OFHEALT H AND HUMAN SERVICES	Procedes et reactifs pour la detection moleculaire de groupes de vih-1 m, n et o
JP4824236B2 (ja)	1999-07-09	2011-11-30	ジェン−プローブ・インコーポレーテッド	核酸増幅によるｈｉｖ−１の検出
US20090075256A1 (en) *	2000-06-17	2009-03-19	Third Wave Technologies, Inc.	Nucleic Acid Accessible Hybridization Site Identification Using Mass Spectrometry
WO2001098537A2 (fr) *	2000-06-17	2001-12-27	Third Wave Technologies, Inc.	Sites d'hybridation accessibles a l'acide nucleique
US6893822B2 (en)	2001-07-19	2005-05-17	Nanogen Recognomics Gmbh	Enzymatic modification of a nucleic acid-synthetic binding unit conjugate
GB0701253D0 (en)	2007-01-23	2007-02-28	Diagnostics For The Real World	Nucleic acid amplification and testing
US8617816B2 (en) *	2007-03-16	2013-12-31	454 Life Sciences, A Roche Company	System and method for detection of HIV drug resistant variants
US20100136516A1 (en) *	2008-12-01	2010-06-03	454 Life Sciences Corporation	System and method for detection of HIV integrase variants
AU2011230619C1 (en)	2010-03-25	2016-06-23	Oregon Health & Science University	CMV glycoproteins and recombinant vectors
HUE037408T2 (hu)	2011-06-10	2018-08-28	Univ Oregon Health & Science	CMV glikoproteinek és rekombináns vektorok
CA2789539A1 (fr)	2011-09-12	2013-03-12	International Aids Vaccine Initiative	Immunoselection de virus de la stomatite vesiculeuse recombinant exprimant des proteines hiv-1 en neutralisant largement les anticorps
ES2631608T3 (es)	2012-06-27	2017-09-01	International Aids Vaccine Initiative	Variante de la glicoproteína Env del VIH-1
CN104428424A (zh)	2012-08-31	2015-03-18	东丽株式会社	目标核酸的检测方法
EP2848937A1 (fr)	2013-09-05	2015-03-18	International Aids Vaccine Initiative	Procédés d'identification de nouveaux immunogènes du VIH-1
US10058604B2 (en)	2013-10-07	2018-08-28	International Aids Vaccine Initiative	Soluble HIV-1 envelope glycoprotein trimers
EP3069730A3 (fr)	2015-03-20	2017-03-15	International Aids Vaccine Initiative	Trimères de glycoprotéines de l'enveloppe du vih-1 soluble
US9931394B2 (en)	2015-03-23	2018-04-03	International Aids Vaccine Initiative	Soluble HIV-1 envelope glycoprotein trimers
CA3298934A1 (en) *	2015-09-18	2026-03-02	Siemens Healthcare Diagnostics Inc.	Reagents and methods for analysis of hiv
US10689689B2 (en) *	2015-12-28	2020-06-23	Roche Molecular Systems, Inc.	Generic method for the stabilization of specific RNA

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU606043B2 (en)	1985-03-28	1991-01-31	F. Hoffmann-La Roche Ag	Detection of viruses by amplification and hybridization
US5008182A (en) *	1986-01-10	1991-04-16	Cetus Corporation	Detection of AIDS associated virus by polymerase chain reaction
JPS63502477A (ja)	1985-12-03	1988-09-22	エル・ギユ−リイ・エム・ラフアツト	病気診断のための方法、装置および製品
US4868105A (en)	1985-12-11	1989-09-19	Chiron Corporation	Solution phase nucleic acid sandwich assay
FR2600342B1 (fr)	1986-06-19	1989-11-17	Eurobio Lab	Procede pour le depistage, chez les individus seropositifs, des sequences d'adn du retrovirus htlv iii-lav et coffret pour sa mise en oeuvre
US5030714A (en)	1986-06-23	1991-07-09	Institut Pasteur	Variant of LAV viruses
WO1988001302A1 (fr)	1986-08-11	1988-02-25	Siska Diagnostics, Inc.	Procedes et compositions d'analyse a l'aide de sondes d'acide nucleique
EP0272098A3 (fr)	1986-12-15	1990-06-06	City Of Hope National Medical Center	Procédé d'amplification et de détection de séquences ARN
JP2948823B2 (ja)	1987-01-16	1999-09-13	アンステイテユ・パストウール	ヒト免疫不全レトロウィルス（ｈｉｖ）関連抗原性ペプチド、そのウィルス感染の検出及びワクチンへの使用
CA1339351C (fr) *	1987-10-15	1997-08-26	Michael S. Urdea	Multimeres d'acide nucleique et essais d'hybridation amplifiee d'acide nucleique
US5124246A (en) *	1987-10-15	1992-06-23	Chiron Corporation	Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5030557A (en) *	1987-11-24	1991-07-09	Ml Technology Venture	Means and method for enhancing nucleic acid hybridization
EP0326395A2 (fr)	1988-01-29	1989-08-02	City Of Hope	Méthode de détection et d'identification de certaines séquences virales
EP0436547B1 (fr)	1988-05-10	1994-11-30	E.I. Du Pont De Nemours And Company	Procede de detection rapide d'acide nucleique
CA2002076A1 (fr)	1988-11-21	1990-05-21	Brent A. Burdick	Trousse de diagnostic et methode utilisant un moyen de capture en phase solide pour la detection des acides nucleiques
WO1990008840A1 (fr)	1989-02-03	1990-08-09	Eastman Kodak Company	Article de test d'un acide nucleique et son utilisation en vue de la detection d'un acide nucleique predetermine
ATE423856T1 (de) *	1989-06-02	2009-03-15	Pasteur Institut	Nukleotid-sequenzen von hiv-1, hiv-2 und siv retrovirusgenomen, ihre verwendung zur amplifizierung von pol-sequenzen dieser retroviren und zur in vitro diagnostik von diesen viren verursachten infektionen
IE910081A1 (en) *	1990-01-10	1991-07-17	Chiron Corp	The use of DNA-dependent RNA polymerase transcripts as¹reporter molecules for signal amplification in nucleic acid¹hybridization assays
FR2663040B1 (fr) *	1990-06-11	1995-09-15	Bio Merieux	Procede de detection d'une sequence nucleotidique selon la technique d'hybridation sandwich.
US5229364A (en) *	1991-03-15	1993-07-20	Francesca Chiodi	Polypeptides derived from the human immunodeficiency virus endonuclease protein

1992
- 1992-12-22 CA CA002124797A patent/CA2124797C/fr not_active Expired - Lifetime
- 1992-12-22 DE DE69233630T patent/DE69233630T2/de not_active Expired - Lifetime
- 1992-12-22 AT AT93902721T patent/ATE329054T1/de active
- 1992-12-22 DK DK93902721T patent/DK0726962T3/da active
- 1992-12-22 JP JP51187393A patent/JP3907201B2/ja not_active Expired - Lifetime
- 1992-12-22 ES ES93902721T patent/ES2267094T3/es not_active Expired - Lifetime
- 1992-12-22 WO PCT/US1992/011168 patent/WO1993013223A1/fr not_active Ceased
- 1992-12-22 EP EP06076034A patent/EP1752545A3/fr not_active Withdrawn
- 1992-12-22 EP EP93902721A patent/EP0726962B1/fr not_active Expired - Lifetime
- 1992-12-22 PT PT93902721T patent/PT726962E/pt unknown
1993
- 1993-01-20 TW TW082100463A patent/TW294722B/zh not_active IP Right Cessation
- 1993-12-17 US US08/169,715 patent/US6300056B1/en not_active Expired - Lifetime
2000
- 2000-08-02 JP JP2000235019A patent/JP2001069997A/ja active Pending
2002
- 2002-04-17 JP JP2002115427A patent/JP2003000286A/ja not_active Withdrawn
2004
- 2004-07-28 JP JP2004220900A patent/JP2004298197A/ja not_active Withdrawn
2006
- 2006-06-29 JP JP2006180511A patent/JP2006254924A/ja not_active Withdrawn

Also Published As

Publication number	Publication date
EP0726962A1 (fr)	1996-08-21
EP0726962B1 (fr)	2006-06-07
US6300056B1 (en)	2001-10-09
JPH07502654A (ja)	1995-03-23
ES2267094T3 (es)	2007-03-01
PT726962E (pt)	2006-10-31
EP0726962A4 (fr)	1998-05-13
JP2003000286A (ja)	2003-01-07
WO1993013223A1 (fr)	1993-07-08
DE69233630T2 (de)	2007-05-31
EP1752545A3 (fr)	2007-12-26
CA2124797C (fr)	2004-03-30
DK0726962T3 (da)	2006-10-09
JP2006254924A (ja)	2006-09-28
EP1752545A2 (fr)	2007-02-14
JP2004298197A (ja)	2004-10-28
JP2001069997A (ja)	2001-03-21
ATE329054T1 (de)	2006-06-15
JP3907201B2 (ja)	2007-04-18
DE69233630D1 (de)	2006-07-20
CA2124797A1 (fr)	1993-07-08

Legal Events

Date	Code	Title	Description
2013-03-01	MK4A	Expiration of patent term of an invention patent

Publication	Publication Date	Title
TW294722B (fr)	1997-01-01
TW293845B (fr)	1996-12-21
TW293844B (fr)	1996-12-21
TW294721B (fr)	1997-01-01
US4868105A (en)	1989-09-19	Solution phase nucleic acid sandwich assay
Brandt et al.	2003	PNA microarrays for hybridisation of unlabelled DNA samples
US5702891A (en)	1997-12-30	HAV probes for use in solution phase sandwich hybridization and assays for detecting the presence of HAV
JP3515781B2 (ja)	2004-04-05	ポリスチレン表面に核酸プローブを固定化する方法
US5849481A (en)	1998-12-15	Nucleic acid hybridization assays employing large comb-type branched polynucleotides
JP3787352B2 (ja)	2006-06-21	ハイブリダイゼーション保護検定
JP5166666B2 (ja)	2013-03-21	型特異的ハイブリッド捕捉法による核酸の検出方法
TW293843B (fr)	1996-12-21
WO2003020985A1 (fr)	2003-03-13	Detection de sequences genetiques au moyen d'une sonde bipartite
JP2001095590A (ja)	2001-04-10	核酸ハイブリダイゼーションアッセイのためのプローブ
CA2747479C (fr)	2017-11-21	Procedes et reactifs pour raccourcir les temps d'incubation dans des dosages par hybridation
JPH02238900A (ja)	1990-09-21	架橋補体を用いるヌクレオチドプローブの製造方法
US20030207289A1 (en)	2003-11-06	Detection of genetic sequences using a bipartite probe