ZA90912B - Assay method - Google Patents

Assay method

Info

Publication number
ZA90912B
ZA90912B ZA90912A ZA90912A ZA90912B ZA 90912 B ZA90912 B ZA 90912B ZA 90912 A ZA90912 A ZA 90912A ZA 90912 A ZA90912 A ZA 90912A ZA 90912 B ZA90912 B ZA 90912B
Authority
ZA
South Africa
Prior art keywords
nucleic acid
assay method
detect
fluorescent
assay
Prior art date
Application number
ZA90912A
Other languages
English (en)
Inventor
Andrew John Garman
John Garman Andrew
Robert Stanley Moore
Stanley Moore Robert
Original Assignee
Ici Plc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ici Plc filed Critical Ici Plc
Publication of ZA90912B publication Critical patent/ZA90912B/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10TTECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00Chemistry: analytical and immunological testing
    • Y10T436/14Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Hybrid Electric Vehicles (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Surgical Instruments (AREA)
  • Transducers For Ultrasonic Waves (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
ZA90912A 1989-02-07 1990-02-07 Assay method ZA90912B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898902689A GB8902689D0 (en) 1989-02-07 1989-02-07 Assay method

Publications (1)

Publication Number Publication Date
ZA90912B true ZA90912B (en) 1990-10-31

Family

ID=10651255

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA90912A ZA90912B (en) 1989-02-07 1990-02-07 Assay method

Country Status (14)

Country Link
US (1) US6022686A (fr)
EP (1) EP0382433B1 (fr)
JP (1) JPH02295496A (fr)
AT (1) ATE159984T1 (fr)
AU (1) AU4902590A (fr)
CA (1) CA2009454C (fr)
DE (1) DE69031665T2 (fr)
ES (1) ES2109226T3 (fr)
FI (1) FI900602A7 (fr)
GB (2) GB8902689D0 (fr)
IE (1) IE900442L (fr)
IL (1) IL93297A0 (fr)
NO (1) NO900582L (fr)
ZA (1) ZA90912B (fr)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US6007984A (en) * 1989-11-01 1999-12-28 Zeneca Limited Detection of DNA/RNA by fluorescence polarization
US5453355A (en) * 1990-01-26 1995-09-26 Abbott Laboratories Oligonucleotides and methods for the detection of Neisseria gonorrhoeae
US5641629A (en) * 1990-06-11 1997-06-24 Nexstar Pharmacueticals Inc Spectroscopically detectable nucleic acid ligands
ATE148503T1 (de) * 1990-09-20 1997-02-15 Amoco Corp Sonde zusammensetzungen für chromosom identifizierung und verfahren
US5620847A (en) * 1990-10-05 1997-04-15 Hoffman-La Roche Inc. Methods and reagents for detection of bacteria in cerebrospinal fluid
EP0533906A4 (en) * 1991-04-11 1994-09-28 Baxter Diagnostics Inc Detection of dna/rna by fluorescence polarization
GB9119735D0 (en) * 1991-09-16 1991-10-30 Secr Defence Gene probe biosensor method
US5567583A (en) * 1991-12-16 1996-10-22 Biotronics Corporation Methods for reducing non-specific priming in DNA detection
US5348853A (en) * 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
US6033854A (en) * 1991-12-16 2000-03-07 Biotronics Corporation Quantitative PCR using blocking oligonucleotides
US5445935A (en) 1992-11-23 1995-08-29 Royer; Catherine A. Quantitative detection of macromolecules with fluorescent oligonucleotides
DE69333991T2 (de) * 1992-11-27 2006-12-14 Canon K.K. Verfahren und Sonde zum Nachweis von Nukleinsäuren
JPH07177899A (ja) * 1993-12-24 1995-07-18 Bio Sensor Kenkyusho:Kk 遺伝子の検出方法
JPH07233065A (ja) * 1993-12-27 1995-09-05 Canon Inc ピリリウム塩又はピリリウム類似塩を含む光化学治療薬
US5547861A (en) * 1994-04-18 1996-08-20 Becton, Dickinson And Company Detection of nucleic acid amplification
CA2145719C (fr) * 1994-04-18 1998-06-30 James G. Nadeau Detection de l'amplification de l'acide nucleique
EP0684239B1 (fr) * 1994-05-26 2003-12-10 Canon Kabushiki Kaisha Méthode de détection de substance, cible dans un échantillon, utilisant un composé de pyrylium
AU705637B2 (en) * 1994-09-23 1999-05-27 Becton Dickinson & Company Detection of nucleic acid amplification
GB9510262D0 (en) * 1995-05-22 1995-07-19 Nat Blood Authority The Detection method
EP1291441A3 (fr) * 1995-09-12 2003-03-19 Becton, Dickinson and Company Dispositif et procédé d'amplification et de dosage d'ADN
US5981180A (en) * 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
DE69638321D1 (de) 1995-10-11 2011-03-03 Luminex Corp Gleichzeitige mehrfachanalyse klinischer proben
US5641633A (en) * 1995-11-15 1997-06-24 Becton, Dickinson And Company Fluorescence polarization detection of nucleic acids
US5800989A (en) * 1995-11-15 1998-09-01 Becton, Dickinson And Company Method for detection of nucleic acid targets by amplification and fluorescence polarization
CA2190430A1 (fr) * 1995-12-12 1997-06-13 Margret Barbara Basinski Methode pour mesurer les messages genetiques
US5723294A (en) * 1996-03-05 1998-03-03 Gull Laboratories Methods for detection and discrimination of multiple analytes using fluorescent technology
DE69718268D1 (de) 1996-10-03 2003-02-13 Canon Kk Verfahren zur Detektion von Zielnukleinsäure, Verfahren zu ihrer Quantifizierung und Pyrylium-Verbindungen zur Chemilumineszenz-Analyse
US6449562B1 (en) 1996-10-10 2002-09-10 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and method
US6503709B1 (en) 1997-07-03 2003-01-07 Id Biomedical Corporation Methods for rapidly detecting methicillin resistant staphylococci
US6013442A (en) * 1997-08-05 2000-01-11 Wisconsin Alumni Res Found Direct quantitation of low copy number RNA
US6261781B1 (en) 1997-08-05 2001-07-17 Wisconsin Alumni Research Foundation Direct detection and mutation analysis of low copy number nucleic acids
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US6982431B2 (en) 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US6289229B1 (en) 1998-01-20 2001-09-11 Scimed Life Systems, Inc. Readable probe array for in vivo use
WO1999060158A1 (fr) * 1998-05-19 1999-11-25 Laboratory Of Molecular Biophotonics Phase solide utilisee pour detecter de l'acide nucleique et procede de detection d'acide nucleique
JP3437094B2 (ja) * 1998-07-03 2003-08-18 松下電器産業株式会社 多波長蛍光偏光法
US6180408B1 (en) * 1998-08-21 2001-01-30 Washington University Fluorescence polarization in nucleic acid analysis
FR2784748A1 (fr) * 1998-10-19 2000-04-21 Revelgenics Detection de reactions de polymerisation par polarisation de fluorescence
US6100039A (en) * 1999-01-28 2000-08-08 Panvera, Inc. Process for reverse transcriptase activity measurement using fluorescence polarization
US6537755B1 (en) 1999-03-25 2003-03-25 Radoje T. Drmanac Solution-based methods and materials for sequence analysis by hybridization
US6777184B2 (en) * 2000-05-12 2004-08-17 Caliper Life Sciences, Inc. Detection of nucleic acid hybridization by fluorescence polarization
US6977161B2 (en) 2000-10-14 2005-12-20 Eragen Biosciences, Inc. Solid support assay systems and methods utilizing non-standard bases
ATE497018T1 (de) 2000-05-19 2011-02-15 Eragen Biosciences Inc Materialien und methoden zum nachweis von nukleinsäuren
US6258546B1 (en) * 2000-06-23 2001-07-10 Becton, Dickinson And Company Detection of nucleic acid amplification
WO2002046453A2 (fr) * 2000-12-05 2002-06-13 Syngenta Participations Ag Procede et trousse d'identification d'enzymes de modification d'acides nucleiques et d'inhibiteurs de tels enzymes
DE10104938B4 (de) * 2001-01-29 2005-06-23 Epigenomics Ag Fluoreszenzpolarisation 1
DE10104937B4 (de) * 2001-01-29 2005-03-17 Epigenomics Ag Fluoreszenzpolarisation 2
US8148171B2 (en) 2001-10-09 2012-04-03 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6589744B2 (en) 2001-11-26 2003-07-08 Syngenta Participations Ag Method and kit for identification for nucleic acid modification enzymes and inhibitors thereof
JP2005538735A (ja) * 2002-09-17 2005-12-22 パーキネルマー ラス インコーポレイテッド 核酸反応のリアルタイム検出法
AU2004206246B2 (en) 2003-01-17 2009-10-01 Luminex Corporation Nucleic acid amplification using non-standard bases
BRPI0408967B8 (pt) 2003-03-31 2021-07-27 Hoffmann La Roche kit e métodos para detecção de um ácido nucléico de vários membros do sorogrupo do vírus da encefalite japonesa em uma amostra biológica sob condições de hibridização rigorosas
WO2004090153A2 (fr) 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Inhibiteur de polymerase et son procede d'utilisation
GB0317592D0 (en) 2003-07-26 2003-08-27 Astrazeneca Ab Method
JP4851188B2 (ja) * 2003-12-19 2012-01-11 日鉄環境エンジニアリング株式会社 核酸測定用新規混合物、及びそれを用いる核酸の新規測定方法並びにそれらに用いる核酸プローブ
US7939251B2 (en) 2004-05-06 2011-05-10 Roche Molecular Systems, Inc. SENP1 as a marker for cancer
US7851152B2 (en) * 2004-09-25 2010-12-14 Yaodong Chen Fluorescent base analogues' usage in the characterization of nucleic acid molecules and their interactions
US20060172314A1 (en) * 2005-01-31 2006-08-03 Song Min-Sun Quantification of amplified nucleic acids
WO2006101913A2 (fr) 2005-03-18 2006-09-28 Eragen Biosciences, Inc. Procedes destines a detecter des especes et des sous-especes multiples de neiserria
JP4650420B2 (ja) * 2005-03-30 2011-03-16 東洋紡績株式会社 塩基判定方法及び塩基判定用キット
US8293472B2 (en) 2005-06-07 2012-10-23 Luminex Corporation Methods for detection and typing of nucleic acids
JP2007330134A (ja) * 2006-06-14 2007-12-27 Toyobo Co Ltd 核酸の検出方法
US9938641B2 (en) * 2006-12-18 2018-04-10 Fluidigm Corporation Selection of aptamers based on geometry
WO2008157649A1 (fr) * 2007-06-18 2008-12-24 Northwestern University Détection non enzymatique d'adn génomique bactérien
WO2010112033A2 (fr) 2009-03-31 2010-10-07 Østjysk Innovation A/S Méthode permettant d'estimer le risque d'avoir ou de développer une sclérose en plaques à l'aide de polymorphismes de séquence dans une région particulière du chromosome x
WO2011050278A1 (fr) 2009-10-23 2011-04-28 Eragen Biosciences, Inc. Amorces d'amplification avec des bases non-standard ayant une spécificité de réaction augmentée
WO2012052758A1 (fr) 2010-10-22 2012-04-26 Astrazeneca Ab Biomarqueurs de réponse pour des antagonistes de iap dans des cancers humains
EP2948566B1 (fr) 2013-01-24 2025-03-26 California Institute of Technology Caractérisation à base de chromophore et procédés de détection
US10077475B2 (en) 2013-01-24 2018-09-18 California Institute Of Technology FRET-based analytes detection and related methods and systems

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4257774A (en) * 1979-07-16 1981-03-24 Meloy Laboratories, Inc. Intercalation inhibition assay for compounds that interact with DNA or RNA
US4668640A (en) * 1981-12-11 1987-05-26 Abbott Laboratories Fluorescence polarization immunoassay utilizing substituted carboxyfluoresceins
US4476228A (en) * 1982-11-08 1984-10-09 Abbott Laboratories Determination of unsaturated thyroxine binding protein sites using fluorescence polarization techniques
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US4670380A (en) * 1984-05-23 1987-06-02 Molecular Diagnostics, Inc. Assays utilizing labeled nucleic acid probes
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4820630A (en) * 1984-11-23 1989-04-11 Digene Diagnostics, Incorporated Assay for nucleic acid sequences, particularly genetic lesions, using interactive labels
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
ES8706823A1 (es) * 1985-03-28 1987-06-16 Cetus Corp Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra
JPS61258168A (ja) * 1985-05-13 1986-11-15 Kogyo Kaihatsu Kenkyusho 核酸配列を同定する方法
US4959463A (en) * 1985-10-15 1990-09-25 Genentech, Inc. Intermediates
EP0232967B1 (fr) * 1986-01-10 1993-04-28 Amoco Corporation Dosage homogène concomitant
US4863876A (en) * 1987-01-15 1989-09-05 Hevey Richard C Method of detecting and quantifying ligands in liquids via biotin-avidin-medicated fluorescence polarization
AU1342488A (en) * 1987-01-15 1988-08-10 Richard C. Hevey Method of detecting and quantifying ligands in liquids
CA1317535C (fr) * 1987-06-30 1993-05-11 Nanibhushan Dattagupta Essai de sequences a l'aide de genes amplifies
PT88665B (pt) * 1987-10-05 1992-12-31 Ml Tecnology Ventures Lp Metodo para a marcacao com ester de acridinio e purificacao de sondas nucleotidicas
US5185439A (en) * 1987-10-05 1993-02-09 Gen-Probe Incorporated Acridinium ester labelling and purification of nucleotide probes
AU622426B2 (en) * 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
JPH0975958A (ja) * 1995-09-06 1997-03-25 Daiei Kogyo Kk オゾン水吐出装置

Also Published As

Publication number Publication date
ES2109226T3 (es) 1998-01-16
CA2009454A1 (fr) 1990-08-07
IE900442L (en) 1990-08-07
US6022686A (en) 2000-02-08
DE69031665D1 (de) 1997-12-11
GB9002536D0 (en) 1990-04-04
EP0382433A2 (fr) 1990-08-16
GB2228998B (en) 1993-04-07
EP0382433B1 (fr) 1997-11-05
FI900602A0 (fi) 1990-02-07
IL93297A0 (en) 1990-11-29
EP0382433A3 (fr) 1992-01-15
ATE159984T1 (de) 1997-11-15
NO900582D0 (no) 1990-02-07
GB8902689D0 (en) 1989-03-30
DE69031665T2 (de) 1998-02-26
FI900602A7 (fi) 1990-08-08
CA2009454C (fr) 2000-04-04
NO900582L (no) 1990-08-08
GB2228998A (en) 1990-09-12
JPH02295496A (ja) 1990-12-06
AU4902590A (en) 1990-08-16

Similar Documents

Publication Publication Date Title
GB2228998B (en) A method for the detection of nucleic acid sequences using fluorescence polarisation
ATE206764T1 (de) Verfahren zum sensitiven nachweis von nukleinsäuren
CA2201595A1 (fr) Compositions et procedes de detection et de quantification simultanees de sequences specifiques multiples d'acide nucleique
IL103754A (en) Rapid assay for detecting nucleic acids sequence in amplification products
DE3480487D1 (en) Nucleic acid hybridization assay
EP0530009A3 (en) Method of characterising genomic dna
ATE94910T1 (de) Verfahren zum nachweis von nucleinsaeuren.
FI950487L (fi) Menetelmä, reagenssi ja testipakkaus nukleiinihapposekvenssien totamiseksi ja monistamiseksi
CA2118913A1 (fr) Essai d'extension d'acides nucleiques
EP0353985A3 (fr) Sonde d'acide nucléique pour la détection de Neisseria gonorrhoea
ATE398186T1 (de) Integriertes verfahren und system zur amplifizierung und zum nachweis von nukleinsäuren
EP0128018A3 (en) Molecular genetic probe, and method of forming same, assay technique and kit using said molecular genetic probe
ES2082881T3 (es) Procedimiento para la deteccion y determinacion cuantitativa de cepas de nitrosomonas en aguas residuales o suelos.
JO1656B1 (en) New method of analysis
AU3535389A (en) Method for diagnosis of infectious diseases and method of detecting and identifying microorganisms
GR3005459T3 (fr)
WO1997020068A3 (fr) Procede de production de plusieurs acides nucleiques a double brin