AR062575A1 - Metodo para la produccion de conjugados del factor de crecimiento similar a la insulina -1 y polietilenglicol - Google Patents

Metodo para la produccion de conjugados del factor de crecimiento similar a la insulina -1 y polietilenglicol

Info

Publication number
AR062575A1
AR062575A1 ARP070103823A ARP070103823A AR062575A1 AR 062575 A1 AR062575 A1 AR 062575A1 AR P070103823 A ARP070103823 A AR P070103823A AR P070103823 A ARP070103823 A AR P070103823A AR 062575 A1 AR062575 A1 AR 062575A1
Authority
AR
Argentina
Prior art keywords
pro
igf
variant
pegylated
production
Prior art date
Application number
ARP070103823A
Other languages
English (en)
Inventor
Joerg Thomas Regula
Hendrik Knoetgen
Andreas Schaubmar
Hans-J Schoenfeld
Christian Schantz
Kart Lang
Friedrich Metzger
Frederike Hesse
Stephan Fischer
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=37667695&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AR062575(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of AR062575A1 publication Critical patent/AR062575A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/56—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule
    • A61K47/59—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes
    • A61K47/60—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic macromolecular compound, e.g. an oligomeric, polymeric or dendrimeric molecule obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyureas or polyurethanes the organic macromolecular compound being a polyoxyalkylene oligomer, polymer or dendrimer, e.g. PEG, PPG, PEO or polyglycerol
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Wood Science & Technology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Diabetes (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Neurology (AREA)
  • Plant Pathology (AREA)
  • Neurosurgery (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Psychiatry (AREA)
  • Hospice & Palliative Care (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Un método para la produccion de IGF-I o variante de IGF-l PEGilado en la lisina, y dicha variante comprende uno o dos aminoácido(s) seleccionados del grupo que consiste en lisina 27, 65 y/o 68 sustituidos independientemente por otro aminoácido polar, que se caracteriza por cultivar una célula huésped procariota que comprende un vector de expresion que contiene un ácido nucleico que codifica una proteína de fusion que comprende dicho IGF-I o variante de IGF-I unido en el extremo N-terminal al extremo C-terminal de un propéptido en el que dicho propéptido finaliza en el C-terminal con los aminoácidos -Y-Pro, en el que Y se selecciona del grupo que consiste en Pro, Pro-Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr-Pro, Arg-Pro, o Pro-Arg- Pro, recuperando y PEGilando dicha proteína de fusion, escindiendo dicha proteína de fusion PEGilada con la proteasa IgA, y recuperando dicho IGF-I o variante de IGF-I PEGilado. El IGF-I o variante de IGF-I PEGilado es util para el tratamiento de trastornos neurodegenerativos como la enfermedad de Alzheimer.
ARP070103823A 2006-08-31 2007-08-29 Metodo para la produccion de conjugados del factor de crecimiento similar a la insulina -1 y polietilenglicol AR062575A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP06018170 2006-08-31

Publications (1)

Publication Number Publication Date
AR062575A1 true AR062575A1 (es) 2008-11-19

Family

ID=37667695

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP070103823A AR062575A1 (es) 2006-08-31 2007-08-29 Metodo para la produccion de conjugados del factor de crecimiento similar a la insulina -1 y polietilenglicol

Country Status (21)

Country Link
US (4) US7625996B2 (es)
EP (1) EP2059261B1 (es)
JP (1) JP5184532B2 (es)
KR (1) KR101106931B1 (es)
CN (1) CN101511390B (es)
AR (1) AR062575A1 (es)
AU (1) AU2007291502B2 (es)
BR (1) BRPI0715943A2 (es)
CA (1) CA2662062C (es)
CL (1) CL2007002502A1 (es)
CO (1) CO6251278A2 (es)
CR (1) CR10591A (es)
ES (1) ES2397660T3 (es)
IL (1) IL196736A (es)
MA (1) MA30660B1 (es)
MX (1) MX2009002011A (es)
NO (1) NO20090451L (es)
PE (1) PE20080912A1 (es)
RU (1) RU2009106118A (es)
TW (1) TW200819468A (es)
WO (1) WO2008025528A1 (es)

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Also Published As

Publication number Publication date
TW200819468A (en) 2008-05-01
WO2008025528A1 (en) 2008-03-06
US20130065831A1 (en) 2013-03-14
CR10591A (es) 2009-04-03
IL196736A (en) 2013-10-31
CO6251278A2 (es) 2011-02-21
KR20090046875A (ko) 2009-05-11
IL196736A0 (en) 2009-11-18
AU2007291502B2 (en) 2012-05-31
EP2059261A1 (en) 2009-05-20
US8476232B2 (en) 2013-07-02
MA30660B1 (fr) 2009-08-03
PE20080912A1 (es) 2008-07-19
NO20090451L (no) 2009-02-26
AU2007291502A1 (en) 2008-03-06
EP2059261B1 (en) 2012-10-24
BRPI0715943A2 (pt) 2013-07-30
CN101511390B (zh) 2013-04-24
CL2007002502A1 (es) 2008-05-30
US20100035817A1 (en) 2010-02-11
MX2009002011A (es) 2009-03-05
CA2662062C (en) 2015-06-23
US20080119409A1 (en) 2008-05-22
JP5184532B2 (ja) 2013-04-17
US7625996B2 (en) 2009-12-01
CN101511390A (zh) 2009-08-19
JP2010501192A (ja) 2010-01-21
RU2009106118A (ru) 2010-10-10
KR101106931B1 (ko) 2012-01-25
CA2662062A1 (en) 2008-03-06
ES2397660T3 (es) 2013-03-08
US20100210547A1 (en) 2010-08-19

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