ATE267267T1 - Verfahren zur quantitativen bestimmung der genexpression mit hilfe der multiplexen competitiven reversen-transkriptase polymerase kettenreaktion - Google Patents

Verfahren zur quantitativen bestimmung der genexpression mit hilfe der multiplexen competitiven reversen-transkriptase polymerase kettenreaktion

Info

Publication number
ATE267267T1
ATE267267T1 AT98929076T AT98929076T ATE267267T1 AT E267267 T1 ATE267267 T1 AT E267267T1 AT 98929076 T AT98929076 T AT 98929076T AT 98929076 T AT98929076 T AT 98929076T AT E267267 T1 ATE267267 T1 AT E267267T1
Authority
AT
Austria
Prior art keywords
gene expression
chain reaction
polymerase chain
reverse transcriptase
transcriptase polymerase
Prior art date
Application number
AT98929076T
Other languages
English (en)
Inventor
James C Willey
Erin L Crawford
Jeffery P Demuth
Clara M Jackson
David A Weaver
Original Assignee
Ohio Med College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ohio Med College filed Critical Ohio Med College
Application granted granted Critical
Publication of ATE267267T1 publication Critical patent/ATE267267T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Electrochemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT98929076T 1997-06-16 1998-06-15 Verfahren zur quantitativen bestimmung der genexpression mit hilfe der multiplexen competitiven reversen-transkriptase polymerase kettenreaktion ATE267267T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/876,766 US5876978A (en) 1993-04-06 1997-06-16 Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
PCT/US1998/012420 WO1998058083A2 (en) 1997-06-16 1998-06-15 Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction

Publications (1)

Publication Number Publication Date
ATE267267T1 true ATE267267T1 (de) 2004-06-15

Family

ID=25368530

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98929076T ATE267267T1 (de) 1997-06-16 1998-06-15 Verfahren zur quantitativen bestimmung der genexpression mit hilfe der multiplexen competitiven reversen-transkriptase polymerase kettenreaktion

Country Status (10)

Country Link
US (1) US5876978A (de)
EP (1) EP0998581B1 (de)
JP (2) JP2001519672A (de)
CN (1) CN1152965C (de)
AT (1) ATE267267T1 (de)
AU (1) AU8072898A (de)
CA (2) CA2482307C (de)
DE (1) DE69824004T2 (de)
RU (1) RU2190019C2 (de)
WO (1) WO1998058083A2 (de)

Families Citing this family (113)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0910666B1 (de) * 1996-05-02 2006-08-09 Applera Corporation Quantifizierung von rna-transkripten mittels genomischer dna als internen standard der amplifizierungsreaktion
US6258543B1 (en) * 1996-05-02 2001-07-10 Xtrana, Inc. Quantitation of RNA transcripts using genomic DNA as the internal amplification competitor
US5853990A (en) 1996-07-26 1998-12-29 Edward E. Winger Real time homogeneous nucleotide assay
US6054276A (en) * 1998-02-23 2000-04-25 Macevicz; Stephen C. DNA restriction site mapping
US6312902B1 (en) 1998-03-13 2001-11-06 Promega Corporation Nucleic acid detection
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6270973B1 (en) 1998-03-13 2001-08-07 Promega Corporation Multiplex method for nucleic acid detection
US6703211B1 (en) 1998-03-13 2004-03-09 Promega Corporation Cellular detection by providing high energy phosphate donor other than ADP to produce ATP
US6391551B1 (en) 1998-03-13 2002-05-21 Promega Corporation Detection of nucleic acid hybrids
US6270974B1 (en) 1998-03-13 2001-08-07 Promega Corporation Exogenous nucleic acid detection
US7090975B2 (en) * 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
US6277578B1 (en) 1998-03-13 2001-08-21 Promega Corporation Deploymerization method for nucleic acid detection of an amplified nucleic acid target
DE19906169A1 (de) * 1999-02-08 2000-08-10 Bioinside Ges Fuer Biodiagnost Testkit und Verfahren zum quantitativen Nachweis von gentechnisch veränderter DNS in Lebensmitteln mittels fluoreszenzgekoppelter PCR
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
EP1257664A4 (de) * 2000-01-28 2006-04-05 Althea Technologies Inc Verfahren zur analyse der genexpression
EP1172445A1 (de) * 2000-07-14 2002-01-16 Praenadia GmbH Verfahren zur direkten genetischen Analyse von Zielzellen anhand Fluoreszentensonden
US8044259B2 (en) 2000-08-03 2011-10-25 The Regents Of The University Of Michigan Determining the capability of a test compound to affect solid tumor stem cells
US6984522B2 (en) * 2000-08-03 2006-01-10 Regents Of The University Of Michigan Isolation and use of solid tumor stem cells
US20080194022A1 (en) * 2000-08-03 2008-08-14 Clarke Michael F Isolation and use of solid tumor stem cells
JP4437171B2 (ja) * 2000-12-12 2010-03-24 独立行政法人放射線医学総合研究所 遺伝子の発現を解析する方法
US20040197785A1 (en) * 2001-03-14 2004-10-07 Willey James C. Method for quantitative measurement of gene expression for indentifying individuals at risk for bronchogenic carcinoma
US7229774B2 (en) 2001-08-02 2007-06-12 Regents Of The University Of Michigan Expression profile of prostate cancer
WO2003057916A2 (en) 2002-01-09 2003-07-17 Riken Cancer profiles
WO2003070935A1 (en) * 2002-02-20 2003-08-28 Sysmex Corporation PRIMERS FOR NUCLEIC ACID AMPLIFICATION IN DETECTING HOUSEKEEPING GENE mRNA AND TEST METHOD USING THESE PRIMERS
US20030186246A1 (en) * 2002-03-28 2003-10-02 Willey James C. Multiplex standardized reverse transcriptase-polymerase chain reacton method for assessment of gene expression in small biological samples
CA2480045C (en) * 2002-03-28 2013-09-24 Medical College Of Ohio Method and compositions for the diagnosis and treatment of non-small cell lung cancer using gene expression profiles
CN1182256C (zh) * 2002-08-09 2004-12-29 周国华 基因表达量比较分析法
EP1594975A4 (de) 2002-12-04 2006-08-02 Applera Corp Multiplex-amplifikation von polynukleotiden
CN1316036C (zh) * 2003-01-06 2007-05-16 徐定邦 一种以聚合酶链式反应为基础的基因定量方法
EP2003196A3 (de) 2003-06-09 2009-01-07 The Regents of the University of Michigan Zusammensetzungen und Verfahren zur Behandlung und Diagnose von Krebs
WO2005000098A2 (en) 2003-06-10 2005-01-06 The Trustees Of Boston University Detection methods for disorders of the lung
US20060024690A1 (en) * 2003-09-19 2006-02-02 Kao H P Normalization of data using controls
US20050112634A1 (en) * 2003-09-19 2005-05-26 Woudenberg Timothy M. High density sequence detection methods and apparatus
US7332280B2 (en) * 2003-10-14 2008-02-19 Ronald Levy Classification of patients having diffuse large B-cell lymphoma based upon gene expression
WO2005074633A2 (en) * 2004-02-03 2005-08-18 The Regents Of The University Of Michigan Compositions and methods for characterizing, regulating, diagnosing, and treating cancer
US7709262B2 (en) * 2004-02-18 2010-05-04 Trustees Of Boston University Method for detecting and quantifying rare mutations/polymorphisms
EP1721011A1 (de) 2004-03-01 2006-11-15 Applera Corporation Verfahren, zusammensetzungen und kits zur verwendung bei der polynukleotidamplifikation
US8304192B2 (en) 2004-03-05 2012-11-06 University Of Toledo Methods and compositions for assessing nucleic acids and alleles
CN100460520C (zh) * 2004-04-05 2009-02-11 周国华 采用碱基序列测定法比较同一基因在不同来源中的表达量的方法
CN1294279C (zh) * 2004-04-05 2007-01-10 周国华 采用碱基序列测定法比较同一基因在不同来源中的表达量的方法
WO2005106036A2 (en) * 2004-04-12 2005-11-10 Medical College Of Ohio Methods and compositions for assaying analytes
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
JP2006014723A (ja) * 2004-06-01 2006-01-19 Sumitomo Chemical Co Ltd コモンマーモセット由来のグリセルアルデヒド３リン酸デヒドロゲナーゼ遺伝子及びその利用
WO2005118872A2 (en) * 2004-06-01 2005-12-15 The Regents Of The University Of Michigan Methods and kits for diagnosing or monitoring autoimmune and chronic inflammatory diseases
US20080152700A1 (en) 2004-06-01 2008-06-26 Reza Sheikhnejad Methods and compositions for the inhibition of gene expression
US20060003337A1 (en) * 2004-06-30 2006-01-05 John Brandis Detection of small RNAS
JP2006042804A (ja) * 2004-07-09 2006-02-16 Sumitomo Chemical Co Ltd コモンマーモセット由来の１８ｓリボソームｒｎａ遺伝子及びその利用
US20080286766A1 (en) * 2004-07-09 2008-11-20 Guohua Zhou Comparing Method for Expression Amount of the Same Gene from Different Sources by Base Sequence Measurement
CA2575675A1 (en) * 2004-07-30 2006-03-09 Adeza Biomedical Corporation Oncofetal fibronectin as a marker for disease and other conditions and methods for detection of oncofetal fibronectin
WO2006079087A2 (en) * 2005-01-21 2006-07-27 Gene Express, Inc. Compositions and methods of use of standardized mixtures
EP2360278A1 (de) 2005-04-14 2011-08-24 Trustees Of Boston University Diagnose für Lungenerkrankungen mittels Klassenvorhersage
CN1862258B (zh) 2005-05-12 2012-05-30 清华大学 核仁素辅助的癌症诊断与治疗方法
US7439024B2 (en) * 2005-06-01 2008-10-21 The United States Of America As Represented By The Department Of Veterans Affairs Methods and kits for diagnosing or monitoring autoimmune and chronic inflammatory diseases
WO2006138275A2 (en) * 2005-06-13 2006-12-28 The Regents Of The University Of Michigan Compositions and methods for treating and diagnosing cancer
US20070099209A1 (en) * 2005-06-13 2007-05-03 The Regents Of The University Of Michigan Compositions and methods for treating and diagnosing cancer
JP2009507229A (ja) * 2005-09-02 2009-02-19 ザ・ユニバーシティ・オブ・トレド 生物学的状態の診断及び／又は治療に有用なバイオマーカーを同定するための方法及び組成物
US8652467B2 (en) * 2005-10-14 2014-02-18 The Regents Of The University Of Michigan Dek protein compositions and methods of using the same
PT2500360E (pt) 2005-10-31 2015-10-15 Oncomed Pharm Inc Composições e métodos para diagnóstico e tratamento do cancro
US7723477B2 (en) 2005-10-31 2010-05-25 Oncomed Pharmaceuticals, Inc. Compositions and methods for inhibiting Wnt-dependent solid tumor cell growth
US7723112B2 (en) * 2005-10-31 2010-05-25 The Regents Of The University Of Michigan Compositions and methods for treating and diagnosing cancer
WO2008121102A2 (en) * 2006-02-21 2008-10-09 The Regents Of The University Of Michigan Hedgehog signaling pathway antagonist cancer treatment
EP2605018A1 (de) 2006-03-09 2013-06-19 The Trustees of the Boston University Diagnose- und Prognoseverfahren für Lungenerkrankungen mittels Genexpressionsprofilen aus Nasenepithelzellen
WO2007115059A2 (en) 2006-03-29 2007-10-11 Merial Limited Vaccine against streptococci
US20080261216A1 (en) * 2006-09-08 2008-10-23 The Regents Of The University Of Michigan HERV Group II Viruses In Lymphoma And Cancer
WO2008045952A2 (en) * 2006-10-10 2008-04-17 The Regents Of The University Of Michigan Photoreceptor precursor cells
WO2008092002A2 (en) * 2007-01-24 2008-07-31 The Regents Of The University Of Michigan Compositions and methods for treating and diagnosing pancreatic cancer
US10745701B2 (en) 2007-06-28 2020-08-18 The Trustees Of Princeton University Methods of identifying and treating poor-prognosis cancers
US20090324596A1 (en) 2008-06-30 2009-12-31 The Trustees Of Princeton University Methods of identifying and treating poor-prognosis cancers
EP3009148B1 (de) 2007-07-02 2018-08-22 Oncomed Pharmaceuticals, Inc. Zusammensetzungen und verfahren zur behandlung und diagnose von krebs
WO2009039190A1 (en) * 2007-09-17 2009-03-26 Gene Express, Inc. Cancer risk biomarker
AU2008302076B2 (en) 2007-09-19 2015-06-11 The Trustees Of Boston University Identification of novel pathways for drug development for lung disease
RU2375458C2 (ru) * 2007-12-25 2009-12-10 Сергей Феликсович Дрозд Способ определения числа копий трансгена в образце днк
US20110183434A1 (en) * 2008-01-17 2011-07-28 Myles Wolf Diagnostic methods and kits using fibroblast growth factor-23
ES2663536T3 (es) 2008-09-26 2018-04-13 Oncomed Pharmaceuticals, Inc. Agentes que se unen a receptores frizzled y usos de los mismos
KR101588547B1 (ko) 2008-11-11 2016-01-28 더 리젠츠 오브 더 유니버시티 오브 미시간 항-ｃｘｃｒ１ 조성물 및 방법
US9495515B1 (en) 2009-12-09 2016-11-15 Veracyte, Inc. Algorithms for disease diagnostics
US10236078B2 (en) 2008-11-17 2019-03-19 Veracyte, Inc. Methods for processing or analyzing a sample of thyroid tissue
US8362318B2 (en) 2008-12-18 2013-01-29 Board Of Trustees Of Michigan State University Enzyme directed oil biosynthesis in microalgae
US8481698B2 (en) * 2009-03-19 2013-07-09 The President And Fellows Of Harvard College Parallel proximity ligation event analysis
EP3360978A3 (de) 2009-05-07 2018-09-26 Veracyte, Inc. Verfahren zur diagnose von schilddrüsenerkrankungen
CA2781595C (en) 2009-07-10 2016-07-05 The Regents Of The University Of Michigan Compositions and methods for diagnosing and treating macular degeneration
US8628924B2 (en) 2009-07-21 2014-01-14 Gen-Probe Incorporated Methods and compositions for quantitative amplification and detection over a wide dynamic range
WO2011025540A1 (en) 2009-08-28 2011-03-03 Bellweather Farms Chemical induction of lactation
WO2011082253A2 (en) 2009-12-30 2011-07-07 Board Of Trustees Of Michigan State University A method to produce acetyldiacylglycerols (ac-tags) by expression ofan acetyltransferase gene isolated from euonymus alatus (burning bush)
TWI535445B (zh) 2010-01-12 2016-06-01 安可美德藥物股份有限公司 Ｗｎｔ拮抗劑及治療和篩選方法
US8574832B2 (en) * 2010-02-03 2013-11-05 Massachusetts Institute Of Technology Methods for preparing sequencing libraries
CN102971337B (zh) 2010-04-01 2016-09-21 昂考梅德药品有限公司 卷曲蛋白结合药剂及其应用
KR20130048217A (ko) 2010-04-06 2013-05-09 메사추세츠 인스티튜트 오브 테크놀로지 적은 수의 전사체 측정치를 이용한 유전자 발현 프로파일링
US9125931B2 (en) 2010-04-06 2015-09-08 Massachusetts Institute Of Technology Post-transcriptional regulation of RNA-related processes using encoded protein-binding RNA aptamers
EP2407242A1 (de) 2010-07-13 2012-01-18 Dublin City University Direkte Klonanalyse und Auswahltechnologie
CA2858526C (en) 2010-12-07 2020-06-30 Northwestern University Molecular targets for als and related disorders
US9657290B2 (en) 2012-07-03 2017-05-23 The Board Of Trustees Of The Leland Stanford Junior University Scalable bio-element analysis
EP2909339B1 (de) * 2012-10-18 2018-05-30 IDEXX Laboratories, Inc. Kits mit kontrollen für nukleinsäureamplifikation
HK1212216A1 (en) 2012-10-23 2016-06-10 Oncomed Pharmaceuticals, Inc. Methods of treating neuroendocrine tumors using wnt pathway-binding agents
ES2675618T3 (es) 2012-11-26 2018-07-11 The University Of Toledo Métodos para la secuenciación estandarizada de ácidos nucleicos y usos de los mismos
US9944973B2 (en) 2012-11-26 2018-04-17 The University Of Toledo Methods for standardized sequencing of nucleic acids and uses thereof
JP2016510411A (ja) 2013-02-04 2016-04-07 オンコメッド ファーマシューティカルズ インコーポレイテッド Ｗｎｔ経路インヒビターによる処置の方法およびモニタリング
US9168300B2 (en) 2013-03-14 2015-10-27 Oncomed Pharmaceuticals, Inc. MET-binding agents and uses thereof
EP2968988A4 (de) 2013-03-14 2016-11-16 Allegro Diagnostics Corp Verfahren zur bewertung eines copd-status
US11976329B2 (en) 2013-03-15 2024-05-07 Veracyte, Inc. Methods and systems for detecting usual interstitial pneumonia
US10392629B2 (en) 2014-01-17 2019-08-27 Board Of Trustees Of Michigan State University Increased caloric and nutritional content of plant biomass
US12297505B2 (en) 2014-07-14 2025-05-13 Veracyte, Inc. Algorithms for disease diagnostics
US20170335396A1 (en) 2014-11-05 2017-11-23 Veracyte, Inc. Systems and methods of diagnosing idiopathic pulmonary fibrosis on transbronchial biopsies using machine learning and high dimensional transcriptional data
EP3037545A1 (de) 2014-12-23 2016-06-29 The Provost, Fellows, Foundation Scholars, & the other members of Board, of the College of the Holy & Undiv. Trinity of Queen Elizabeth near Dublin DNS-Methylierungstest auf Prostatakrebs
US10526600B2 (en) 2015-02-22 2020-01-07 The Board Of Trustees Of The Leland Stanford Junior University Micro-screening apparatus, process, and products
EP4180531A3 (de) 2016-05-12 2023-08-23 Trustees of Boston University Genexpressionssignatur des nasenepithels und klassifikator zur vorhersage von lungenkrebs
US11118233B2 (en) 2016-05-18 2021-09-14 The University Of Chicago BTK mutation and ibrutinib resistance
US10927417B2 (en) 2016-07-08 2021-02-23 Trustees Of Boston University Gene expression-based biomarker for the detection and monitoring of bronchial premalignant lesions
ES2989473T3 (es) 2016-11-14 2024-11-26 Orca Biosystems Inc Aparatos para clasificar partículas objetivo
US10815484B2 (en) 2017-11-22 2020-10-27 The Regents Of The University Of Michigan Compositions and methods for treating cancer
EP3788377A1 (de) 2018-05-04 2021-03-10 Abbott Laboratories Hbv-diagnostische, prognostische und therapeutische verfahren und produkte
WO2020102513A1 (en) 2018-11-14 2020-05-22 Arizona Board Of Regents On Behalf Of The University Of Arizona Systems and methods for characterizing and treating cancer

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5643765A (en) * 1993-04-06 1997-07-01 University Of Rochester Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chain reaction
DE69429038T2 (de) * 1993-07-28 2002-03-21 Pe Corporation (Ny), Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung

Also Published As

Publication number Publication date
WO1998058083A3 (en) 1999-03-11
EP0998581B1 (de) 2004-05-19
DE69824004D1 (de) 2004-06-24
CA2294470C (en) 2005-09-06
AU8072898A (en) 1999-01-04
RU2190019C2 (ru) 2002-09-27
CA2482307A1 (en) 1998-12-23
JP2001519672A (ja) 2001-10-23
JP2005095179A (ja) 2005-04-14
EP0998581A2 (de) 2000-05-10
CN1265155A (zh) 2000-08-30
DE69824004T2 (de) 2005-06-02
CA2294470A1 (en) 1998-12-23
US5876978A (en) 1999-03-02
CA2482307C (en) 2009-05-12
WO1998058083A2 (en) 1998-12-23
CN1152965C (zh) 2004-06-09

Similar Documents

Publication Publication Date Title
ATE267267T1 (de) Verfahren zur quantitativen bestimmung der genexpression mit hilfe der multiplexen competitiven reversen-transkriptase polymerase kettenreaktion
DE69714630D1 (de) Biosensor und Verfahren zur quantitaven Analyse von biochemischen Substraten
DE69820111D1 (de) Massenspektroskopische verfahren zur sequenzierung von nukleinsäuren
ATE409236T1 (de) Derivatisierte nukleinsäuren und deren verwendung
DE69910007D1 (de) Vorrichtung zum vorhersagen von physiologischen messwerten
ATE232559T1 (de) Verfahren zur signalverstärkung
DK0604506T3 (da) Genprobebiosensorfremgangsmåde
DK0979308T3 (da) Fremgangsåde til fremstilling af et diagnostisk standard gen-transkriptmönster
ATE178656T1 (de) Bestimmung der menge an nukleinsäuren unter verwendung der polymerasekettenreaktion
DE69723860D1 (de) VERFAHREN ZUR BESTIMMUNG VON IgE
DK0975800T3 (da) Analyse af nukleotider i opløsning under anvendelse af PNA-prober
DE69632252D1 (de) Verfahren zur erkennung von klonalen populationen von transformierten zellen in einer genomisch heterogenen zellulären probe
ATE111961T1 (de) Verfahren zur bestimmung von polymerase- aktivität.
ATE321886T1 (de) Oligonukleotide und diese enthaltende komplexe zur detektion der anwesenheit oder abwesenheit einer zielnukleinsäure in einer probe
FI910822A7 (fi) Menetelmä DNA-polymeraasin määrittämiseksi kvantitatiivisesti ja siinäkäyttökelpoinen koepakkaus
DE60029413D1 (de) Enzymatische messung von mycophenolsäure
ATE347105T1 (de) Verfahren, assay und kit zur quantifizierung von hiv-proteasehemmer
DE69604051D1 (de) Verfahren zum sensitiven nachweis von reverser transcriptase
ATE238551T1 (de) Homogene nachweisverfahren zur bestimmung der phosphorylierungsaktivitäten von biologischem material
DE59814359D1 (de) Mikrostrukturierter biosensor, verwendung des biosensors und verfahren zur immobilisierung von biokatalysatoren
DE60043877D1 (de) Verfahren zur Bestimmung von CTp11 sowie zur Bestimmung des metastatischen Potentials einer Tumor Probe
DE60136572D1 (de) Detektion von rpob sequenzen von mycobacterium tuberculosis

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0998581

Country of ref document: EP

REN Ceased due to non-payment of the annual fee