ATE305034T1 - Transkriptionsmodulatoren tetracyklin-regulierte - Google Patents

Transkriptionsmodulatoren tetracyklin-regulierte

Info

Publication number
ATE305034T1
ATE305034T1 AT95926605T AT95926605T ATE305034T1 AT E305034 T1 ATE305034 T1 AT E305034T1 AT 95926605 T AT95926605 T AT 95926605T AT 95926605 T AT95926605 T AT 95926605T AT E305034 T1 ATE305034 T1 AT E305034T1
Authority
AT
Austria
Prior art keywords
tet operator
tetracycline
analogue
absence
genes
Prior art date
Application number
AT95926605T
Other languages
English (en)
Inventor
Hermann Bujard
Manfred Gossen
Original Assignee
Tet Systems Holding Gmbh & Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/275,876 external-priority patent/US5654168A/en
Priority claimed from US08/383,754 external-priority patent/US5789156A/en
Application filed by Tet Systems Holding Gmbh & Co filed Critical Tet Systems Holding Gmbh & Co
Application granted granted Critical
Publication of ATE305034T1 publication Critical patent/ATE305034T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/71—Fusion polypeptide containing domain for protein-protein interaction containing domain for transcriptional activaation, e.g. VP16
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
    • C12N2710/16622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/002—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
    • C12N2830/003—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor tet inducible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/001—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination
    • C12N2830/005—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB
    • C12N2830/006—Vector systems having a special element relevant for transcription controllable enhancer/promoter combination repressible enhancer/promoter combination, e.g. KRAB tet repressible
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/32—Vector systems having a special element relevant for transcription being an silencer not forming part of the promoter region

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Environmental Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Veterinary Medicine (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT95926605T 1994-07-01 1995-06-29 Transkriptionsmodulatoren tetracyklin-regulierte ATE305034T1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US27063794A 1994-07-01 1994-07-01
US08/275,876 US5654168A (en) 1994-07-01 1994-07-15 Tetracycline-inducible transcriptional activator and tetracycline-regulated transcription units
US08/383,754 US5789156A (en) 1993-06-14 1995-02-03 Tetracycline-regulated transcriptional inhibitors
US08/486,814 US5866755A (en) 1993-06-14 1995-06-07 Animals transgenic for a tetracycline-regulated transcriptional inhibitor
PCT/US1995/008179 WO1996001313A1 (en) 1994-07-01 1995-06-29 Tetracycline-regulated transcriptional modulators

Publications (1)

Publication Number Publication Date
ATE305034T1 true ATE305034T1 (de) 2005-10-15

Family

ID=27501005

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95926605T ATE305034T1 (de) 1994-07-01 1995-06-29 Transkriptionsmodulatoren tetracyklin-regulierte

Country Status (13)

Country Link
US (2) US5866755A (de)
EP (2) EP1092771B1 (de)
JP (3) JP4054058B2 (de)
CN (2) CN1167504A (de)
AT (1) ATE305034T1 (de)
AU (1) AU3092395A (de)
CA (1) CA2193122C (de)
DE (2) DE804565T1 (de)
DK (1) DK0804565T3 (de)
ES (1) ES2139552T3 (de)
FI (1) FI965287A7 (de)
NO (1) NO315375B1 (de)
WO (1) WO1996001313A1 (de)

Families Citing this family (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5912411A (en) * 1993-06-14 1999-06-15 University Of Heidelberg Mice transgenic for a tetracycline-inducible transcriptional activator
US5888981A (en) 1993-06-14 1999-03-30 Basf Aktiengesellschaft Methods for regulating gene expression
CA2165162C (en) 1993-06-14 2000-05-23 Hermann Bujard Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5859310A (en) 1993-06-14 1999-01-12 Basf Aktiengesellschaft Mice transgenic for a tetracycline-controlled transcriptional activator
US5589362A (en) * 1993-06-14 1996-12-31 Basf Aktiengesellschaft Tetracycline regulated transcriptional modulators with altered DNA binding specificities
FR2732348B1 (fr) * 1995-03-31 1997-04-30 Rhone Poulenc Rorer Sa Systeme d'expression conditionnel
US5851796A (en) * 1995-06-07 1998-12-22 Yale University Autoregulatory tetracycline-regulated system for inducible gene expression in eucaryotes
FR2735789B1 (fr) 1995-06-23 1997-07-25 Centre Nat Rech Scient Adenovirus recombinants, leur utilisation pour preparer des aav, lignee cellulaire complementaire et compositions pharmaceutiques les contenant
GB9526509D0 (en) * 1995-12-23 1996-02-28 Univ Dundee Gene control
DE19635568C1 (de) * 1996-09-02 1998-03-26 Gsf Forschungszentrum Umwelt Vektorsysteme zur konditionalen Genexpression
CA2236867A1 (en) * 1996-09-27 1998-04-02 Icos Corporation Method to identify compounds for disrupting protein/protein interactions
US7008776B1 (en) 1996-12-06 2006-03-07 Aventis Pharmaceuticals Inc. Compositions and methods for effecting the levels of high density lipoprotein (HDL) cholesterol and apolipoprotein AI very low density lipoprotein (VLDL) cholesterol and low density lipoprotein (LDL) cholesterol
WO1998037185A2 (en) * 1997-02-20 1998-08-27 The Board Of Regents Of The University Of Texas System Vectors for controlled gene expression
DE19707493C1 (de) * 1997-02-25 1998-02-26 Univ Ludwigs Albert Nucleinsäurekonstrukte zur lang andauernden Expression von Transgenen
EP0973928B1 (de) 1997-03-11 2010-05-05 Regents Of The University Of Minnesota Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle
US6638502B1 (en) 1997-04-28 2003-10-28 Gencell Sas Adenovirus-mediated intratumoral delivery of an angiogenesis antagonist for the treatment of tumors
US6087166A (en) 1997-07-03 2000-07-11 Basf Aktiengesellschaft Transcriptional activators with graded transactivation potential
EP1005486A4 (de) * 1997-08-22 2004-09-29 Univ Washington Induzierbare regulatorische systeme und deren verwendung
US5885779A (en) * 1997-09-09 1999-03-23 University Of British Columbia Repressed trans-activator system for characterization of protein-protein interactions
US6576469B1 (en) 1997-09-10 2003-06-10 President And Fellows Of Harvard College Inducible methods for repressing gene function
AU748334B2 (en) 1997-10-16 2002-05-30 Board Of Regents, The University Of Texas System Models and treatments for cardiac hypertrophy in relation with NF-AT3 function
EP1721986A1 (de) * 1997-10-29 2006-11-15 Genetics Institute, LLC Schnellerzeugung von stabilen Säugertier-Zelllinien mit Gewinnung eines hohen Gehalts von rekombinanten Proteinen
US6136536A (en) * 1997-10-29 2000-10-24 Genetics Institute, Inc. Rapid generation of stable mammalian cell lines producing high levels of recombinant proteins
US5968773A (en) * 1997-11-14 1999-10-19 Heddle; John A. System and method for regulation of gene expression
NZ503859A (en) * 1997-11-18 2003-02-28 Pioneer Hi Bred Int Non-identical minimal recombination sites (FRT) which are non-identical for targeted integration of nucleotide sequences into transformed plants
DE19751587A1 (de) * 1997-11-21 1999-07-29 Hoechst Marion Roussel De Gmbh Onkogen- oder virusgesteuerte Expressionssysteme
US6153383A (en) * 1997-12-09 2000-11-28 Verdine; Gregory L. Synthetic transcriptional modulators and uses thereof
DE19756975A1 (de) 1997-12-20 1999-06-24 Hoechst Marion Roussel De Gmbh Bindungspartner für Inhibitoren von cyclinabhängigen Kinasen und ihre Verwendung zur Suche nach Inhibitoren, zur Diagnose oder zur Therapie einer Erkrankung
JP2002500039A (ja) 1998-01-08 2002-01-08 アベンティス ファーマスーティカルズ プロダクツ インコーポレイテッド 機能あるヒトリポタンパク質（ａ）を発現するトランスジェニックウサギ
AU2312699A (en) * 1998-01-16 1999-08-02 Pharmacia & Upjohn Company An autoregulatory system for validating microbial genes as possible antimicrobial targets using tetracycline-controllable element
US6878516B1 (en) 1998-01-16 2005-04-12 Pharmacia & Upjohn Company Autoregulatory system for validating microbial genes as possible antimicrobial targets using a tetracycline-controllable element
US7160682B2 (en) 1998-11-13 2007-01-09 Regents Of The University Of Minnesota Nucleic acid transfer vector for the introduction of nucleic acid into the DNA of a cell
AU770893B2 (en) * 1999-02-09 2004-03-04 Rhobio A method for inhibiting the expression of target genes in plants
US6291226B1 (en) 1999-02-25 2001-09-18 National Research Council Of Canada Adenovirus mutants with deleted protease gene
US6890726B1 (en) 1999-04-06 2005-05-10 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
US6287813B1 (en) 1999-04-23 2001-09-11 Cistronics Cell Technology Gmbh Antibiotic-based gene regulation system
CA2373121A1 (en) * 1999-05-11 2000-11-16 Regents Of The University Of Minnesota Vector-mediated delivery of integrating transposon sequences
AU778150B2 (en) * 1999-05-28 2004-11-18 Gendaq Limited Molecular switches
ATE458818T1 (de) * 1999-06-07 2010-03-15 Tet Systems Holding Gmbh & Co Tet repressor basierte transkriptionale regulatorische proteine
PT1210428E (pt) 1999-08-23 2015-07-21 Genetics Inst Llc Pd-1, um recetor para b7-4 e suas utilizações
US6632608B2 (en) 1999-12-30 2003-10-14 President And Fellows Of Harvard College Methods and compositions relating to modulation of hepatocyte growth, plasma cell differentiation or T cell subset activity by modulation of XBP-1 activity
CA2408826C (en) * 2000-05-12 2012-08-14 The Johns Hopkins University A method for generating hypermutable organisms
US20040204379A1 (en) * 2000-06-19 2004-10-14 Cheng Seng H. Combination enzyme replacement, gene therapy and small molecule therapy for lysosomal storage diseases
FR2811681A1 (fr) * 2000-07-17 2002-01-18 Commissariat Energie Atomique Procede de criblage de la translocation nucleo-cytoplasmique de proteines sous l'effet d'un stimulus, d'elements y etant impliques et kits de mise en oeuvre
FR2813085A1 (fr) * 2000-08-18 2002-02-22 Aventis Pharma Sa Systeme de regulation in vivo de l'expression d'un transgene par inhibition conditionnelle
IL154508A0 (en) * 2000-08-18 2003-09-17 Gencell Sa System for regulating in vivo the expression of a transgene by conditional inhibition
FR2814642B1 (fr) 2000-10-03 2005-07-01 Ass Pour Le Dev De La Rech En Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee
CA2443266A1 (en) * 2001-02-13 2002-08-22 University Of Florida A bi-directional dual promoter complex with enhanced promoter activity for transgene expression in eukaryotes
WO2002079499A1 (en) 2001-04-02 2002-10-10 Wyeth Pd-1, a receptor for b7-4, and uses therefor
WO2002088346A2 (en) 2001-05-01 2002-11-07 National Research Council Of Canada A system for inducible expression in eukaryotic cells
US20030221203A1 (en) * 2001-10-03 2003-11-27 Agha-Mohammadi Siamak High efficiency regulatable gene expression system
WO2003054148A2 (en) * 2001-12-07 2003-07-03 University Of Massachusetts Zinc finger-based drug-dependent gene regulation system
EP1497439B1 (de) * 2002-04-15 2006-09-06 Universitätsklinikum Hamburg-Eppendorf Rekombinante virale vektoren zur tetracyclinregulierbaren genexpression
US8227432B2 (en) 2002-04-22 2012-07-24 Regents Of The University Of Minnesota Transposon system and methods of use
AU2003237259B2 (en) * 2002-05-29 2008-01-24 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
ATE417116T1 (de) * 2002-08-28 2008-12-15 Tet Systems Holding Gmbh & Co Chromosomstellen zur stringenten kontrolle von genaktivität durch transkriptionsteuersysteme
FR2845692A1 (fr) * 2002-10-15 2004-04-16 France Hybrides Procede pour produire un mammifere rendu resistant a une infection par un alpha herpes virus par transgenese germinale ainsi que mammifere obtenu par la mise en oeuvre de ce procede
WO2004061077A2 (en) * 2002-12-24 2004-07-22 North Carolina State University Increasing gamete production with a gene switch
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
US8507277B2 (en) 2003-10-24 2013-08-13 Gencia Corporation Nonviral vectors for delivering polynucleotides
US8062891B2 (en) 2003-10-24 2011-11-22 Gencia Corporation Nonviral vectors for delivering polynucleotides to plants
US20090123468A1 (en) 2003-10-24 2009-05-14 Gencia Corporation Transducible polypeptides for modifying metabolism
US20090208478A1 (en) * 2003-10-24 2009-08-20 Gencia Corporation Transducible polypeptides for modifying metabolism
US8133733B2 (en) 2003-10-24 2012-03-13 Gencia Corporation Nonviral vectors for delivering polynucleotides to target tissues
WO2005056752A2 (en) 2003-10-24 2005-06-23 Gencia Corporation Methods and compositions for delivering polynucleotides
WO2005046450A2 (en) 2003-11-12 2005-05-26 Children's Hospital Medical Center Method for diagnosis and treatment of pulmonary disorders
EP1627563A1 (de) 2004-08-10 2006-02-22 Academisch Medisch Centrum bij de Universiteit van Amsterdam Verfahren und Mittel zur Herstellung einer stabilisierten Zelle
JP4944781B2 (ja) * 2005-09-15 2012-06-06 独立行政法人科学技術振興機構 テトラサイクリン系抗生物質によるタンパク質分解制御法
CA2623106C (en) 2005-09-19 2013-12-24 Histogenics Corporation Cell-support matrix having narrowly defined uniformly vertically and non-randomly organized porosity and pore density and a method for preparation thereof
JP5155176B2 (ja) * 2005-11-17 2013-02-27 ティーイーティー システムズ ゲーエムベーハー ウント ツェーオー． カーゲー 誘導発現系
USRE49583E1 (en) 2005-11-17 2023-07-18 Tet Systems Gmbh & Co. Kg Inducible expression systems
DK1974017T4 (da) 2005-12-09 2023-09-25 Academisch Medisch Centrum Bij De Univ Van Amsterdam Midler og fremgangsmåder til at påvirke stabiliteten af antistof producerende celler
WO2007067032A1 (en) 2005-12-09 2007-06-14 Academisch Medisch Cemtrum Bij De Universiteit Van Amsterdam Means and methods for influencing the stability of cells
CA2652347A1 (en) 2006-05-15 2007-11-22 Paratek Pharmaceuticals, Inc. Methods of regulating expression of genes or of gene products using substituted tetracycline compounds
US20080300202A1 (en) * 2006-05-18 2008-12-04 The State of Oregon acting by and through the State Board of Higher Education on behalf of the Subtractive transgenics
AU2007276793B2 (en) 2006-07-28 2014-01-16 Sanofi Composition and method for treatment of tumors
ATE498008T1 (de) * 2006-10-23 2011-02-15 Res Org Information & Systems Verfahren zur herstellung eines zellstamms mit fähigkeit zur tetracyclin-induzierten genexpression oder eines zellstamms mit konditionalem gen-knockout sowie verwendung der zellstämme
EP2135945B1 (de) * 2007-03-14 2014-04-23 Japan Science and Technology Agency Verfahren zur doppelten regulation von transkription/abbau für ein protein mittels antibiotikum
CA2685714C (en) 2007-05-31 2016-04-12 University Of Washington Inducible mutagenesis of target genes
DK2352833T3 (da) 2008-10-01 2013-04-22 Tet Systems Gmbh & Co Kg Tetracyclin inducerbar transkriptionskontrolsekvens
EP2364322A1 (de) * 2008-10-28 2011-09-14 Pioneer Hi-Bred International Inc. Sulfonylharnstoffreaktive repressorproteine
EP2350264A4 (de) 2008-11-06 2012-08-29 Univ Johns Hopkins Behandlung von chronisch-entzündlichen atemwegserkrankungen
DK2454283T3 (en) 2009-07-15 2018-05-07 Aimm Therapeutics Bv METHODS AND PROCEDURES FOR MANUFACTURING HIGH EFFICIENCY ANTIBODIES
WO2011088163A1 (en) 2010-01-14 2011-07-21 President And Fellows Of Harvard College Methods for modulating skeletal remodeling and patterning by modulating shn2 activity, shn3 activity, or shn2 and shn3 activity in combination
EP2366796A1 (de) * 2010-03-09 2011-09-21 Friedrich-Alexander-Universität Erlangen-Nürnberg Mittel und Verfahren zur Identifizierung von Verbindungen, die die Zellkernlokalisierung von Proteinen verändern
WO2011153440A2 (en) 2010-06-04 2011-12-08 Regents Of The University Of Minnesota Transposon mutagenesis system and methods of use
WO2012051301A1 (en) 2010-10-12 2012-04-19 President And Fellows Of Harvard College Methods for identifying modulators of triglyceride metabolism, for modulating triglyceride metabolism and for identifying subjects at risk for abnormal triglyceride metabolism
US9127283B2 (en) 2010-11-24 2015-09-08 Clontech Laboratories, Inc. Inducible expression system transcription modulators comprising a distributed protein transduction domain and methods for using the same
ES2626671T3 (es) 2010-12-02 2017-07-25 Aimm Therapeutics B.V. Medios y métodos para producir anticuerpos de alta afinidad
WO2012109238A2 (en) 2011-02-07 2012-08-16 President And Fellows Of Harvard College Methods for increasing immune responses using agents that directly bind to and activate ire-1
EP2690950B1 (de) 2011-03-29 2017-01-04 Dynavax Technologies Corporation Transgene tlr8-tiere
WO2012156535A1 (en) 2011-05-19 2012-11-22 Fundación Progreso Y Salud Highly inducible dual-promoter lentiviral tet-on system
EP2750715B1 (de) 2011-08-30 2018-10-31 The Regents of The University of California Identifikation von kleinen molekülen zur verstärkung eines therapeutischen exon-skippings
JP5871304B2 (ja) * 2011-09-06 2016-03-01 大学共同利用機関法人自然科学研究機構 テトラサイクリン遺伝子発現誘導システムにおける発現量を増幅させる遺伝子座とノックインによる増幅の効果
ES2523016B1 (es) 2013-05-20 2015-09-09 3P Biopharmaceuticals Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes
US9957506B2 (en) 2013-09-25 2018-05-01 Cornell University Compounds for inducing anti-tumor immunity and methods thereof
EP2878674A1 (de) 2013-11-28 2015-06-03 Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC) Stabile Episomen auf Basis von nichtintegrativen lentiviralen Vektoren
WO2015115892A1 (en) 2014-01-31 2015-08-06 Aimm Therapeutics B.V. Means and methods for producing stable antibodies
US10077420B2 (en) 2014-12-02 2018-09-18 Histogenics Corporation Cell and tissue culture container
WO2017109654A1 (en) * 2015-12-23 2017-06-29 Basf Se Pamamycin controlled systems for gene expression
CN107344962B (zh) * 2016-05-04 2021-03-02 中国科学院微生物研究所 阻遏蛋白、调控元件组和基因表达调控系统及其构建方法
CN108823208B (zh) * 2018-06-29 2021-11-02 中国科学院过程工程研究所 一种四环素诱导启动子及其制备方法和应用
KR20210031898A (ko) 2018-07-13 2021-03-23 난징 레전드 바이오테크 씨오., 엘티디. 전염병을 치료하기 위한 공동수용체 시스템
IL282875B2 (en) 2018-11-08 2026-05-01 Sutro Biopharma Inc E. coli strains with oxygenated cytoplasm
JP7810554B2 (ja) 2019-03-01 2026-02-03 アケロイス バイオファーマ， インコーポレイテッド 抗原特異的免疫細胞におけるｃｄ１６０機能をモジュレートする方法およびその使用
CN113699147B (zh) 2020-05-22 2023-06-09 深圳市深研生物科技有限公司 基于四环素和Cumate的共调控序列
JP2023544254A (ja) 2020-09-14 2023-10-23 ストロ バイオファーマ， インコーポレイテッド 無細胞タンパク質合成系を用いた抗体の大規模産生方法
US11987795B2 (en) 2020-11-24 2024-05-21 The Broad Institute, Inc. Methods of modulating SLC7A11 pre-mRNA transcripts for diseases and conditions associated with expression of SLC7A11
US11566262B2 (en) 2021-03-24 2023-01-31 Dna Twopointo Inc. Tetracycline-inducible expression systems
US20240207449A1 (en) * 2021-04-09 2024-06-27 Washington University Compositions and methods for treatment of crx-mediated retinopathies
WO2022247873A1 (zh) 2021-05-27 2022-12-01 中国科学院动物研究所 工程化的Cas12i核酸酶、效应蛋白及其用途
CN114085874B (zh) * 2022-01-10 2022-04-29 广东乾晖生物科技有限公司 一种制备肝功能可逆的永生化肝细胞的方法及其应用
EP4689127A1 (de) 2023-04-03 2026-02-11 Sutro Biopharma, Inc. Rf1 ko e. coli stämme
WO2025091009A2 (en) 2023-10-27 2025-05-01 Sutro Biopharma, Inc. Overexpression of lysozyme in t7 rna polymerase-expressing host cells

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4833080A (en) * 1985-12-12 1989-05-23 President And Fellows Of Harvard College Regulation of eucaryotic gene expression
FR2628453B1 (fr) * 1988-03-08 1990-11-16 Cofpa Procede de pose d'une toile autour d'un cylindre et dispositif de pre-tension pour la mise en oeuvre du procede
US5221778A (en) * 1988-08-24 1993-06-22 Yale University Multiplex gene regulation
GB8901676D0 (en) * 1989-01-26 1989-03-15 Ici Plc Regulation of gene expression
EP0484440A4 (en) * 1989-07-28 1993-01-07 Us Health Efficient directional genetic cloning system
WO1991013979A1 (en) * 1990-03-05 1991-09-19 President And Fellows Of Harvard College Transgenic mouse overexpressing il-4 and method of use
EP0455424A3 (en) * 1990-05-02 1992-04-29 Merck & Co. Inc. Mammalian inducible promoter cascade system
AU654284B2 (en) * 1990-06-12 1994-11-03 Baylor College Of Medicine Method for homologous recombination in animal and plant cells
WO1991019784A1 (en) * 1990-06-13 1991-12-26 The Trustees Of Princeton University A Lac REPRESSOR-HSV VP16 CHIMERIC TRANSCRIPTIONAL ACTIVATOR PROTEIN SYSTEM FUNCTIONING IN TRANSFECTED MAMMALIAN CELLS
JPH06503967A (ja) * 1991-01-04 1994-05-12 ベイラー・カレッジ・オブ・メディシン 腫瘍感受性非ヒト動物
DE4100594A1 (de) * 1991-01-08 1992-07-09 Inst Genbiologische Forschung Neue plasmide zur zeitlichen und oertlichen kontrollierten expression eines heterologen produktes in pflanzen
ES2163398T3 (es) * 1991-03-15 2002-02-01 Hsc Res Dev Ltd Partnership Construccion genica para la produccion de peces transgenicos.
AU664847B2 (en) * 1991-05-15 1995-12-07 Cell Genesys, Inc. Genomic modifications with homologous DNA targeting
WO1993004169A1 (en) * 1991-08-20 1993-03-04 Genpharm International, Inc. Gene targeting in animal cells using isogenic dna constructs
AU685054C (en) * 1992-05-14 2003-02-27 Baylor College Of Medicine Mutated steroid hormone receptors, methods for their use and molecular switch for gene therapy
CA2143326A1 (en) * 1992-08-26 1994-03-03 Guerard Byrne Tetracycline repressor-mediated binary regulation system for control of gene expression in transgenic animals
EP1978095A1 (de) * 1993-02-12 2008-10-08 The Board Of Trustees Of The Leland Stanford Junior University Regulierte Transkription gezielter Gene und anderer biologischer Ereignisse
CA2165162C (en) * 1993-06-14 2000-05-23 Hermann Bujard Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5464758A (en) * 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters

Also Published As

Publication number Publication date
US5866755A (en) 1999-02-02
JP4682176B2 (ja) 2011-05-11
JP4820344B2 (ja) 2011-11-24
JPH11506901A (ja) 1999-06-22
EP1092771A2 (de) 2001-04-18
DE69534472T2 (de) 2006-06-14
EP0804565B1 (de) 2005-09-21
JP2007295938A (ja) 2007-11-15
EP1092771B1 (de) 2012-12-12
ES2139552T3 (es) 2006-04-16
DE804565T1 (de) 2000-05-04
CN1167504A (zh) 1997-12-10
JP2008011862A (ja) 2008-01-24
NO965623L (no) 1997-02-28
AU3092395A (en) 1996-01-25
DE69534472D1 (de) 2006-02-02
FI965287A0 (fi) 1996-12-31
EP0804565A1 (de) 1997-11-05
DK0804565T3 (da) 2006-02-06
ES2139552T1 (es) 2000-02-16
NO315375B1 (no) 2003-08-25
JP4054058B2 (ja) 2008-02-27
WO1996001313A1 (en) 1996-01-18
CN1523112A (zh) 2004-08-25
NO965623D0 (no) 1996-12-30
US20040003417A1 (en) 2004-01-01
CA2193122A1 (en) 1996-01-18
CA2193122C (en) 2005-08-23
FI965287A7 (fi) 1997-02-28
EP1092771A3 (de) 2001-08-01

Similar Documents

Publication Publication Date Title
DE69534472D1 (de) Transkriptionsmodulatoren tetracyklin-regulierte
DK2050818T3 (da) Transkriptionsaktivatorer med gradueret transaktiveringspotentiale
DE69434663D1 (de) Nukleotid-sequenzen zur kontrolle der exprimierung von dns-sequenzen in einem zellulärem wirt
ATE66693T1 (de) Rekombinante dns-sequenzen, diese enthaltende vektoren und verfahren zur verwendung derselben.
BR0214350A (pt) Sequências de ácido nucleico e aminoácido de adenovìrus de sìmio, vetores contendo as mesmas e métodos de uso
ATE400651T1 (de) Verwendung von grünem fluoreszenzprotein
ATE352638T1 (de) REVERSE ßTWO-HYBRIDß-SYSTEME
ATE214095T1 (de) Verwendung einer exo-nukleotidprobe in der genklonierung
DE69333815D1 (de) Gentranskription und ionisierende strahlung: methoden und zusammensetzungen
DE60228477D1 (de) Verfahren zur regulierung der immunfunktion in primaten unter verwendung des foxp3-proteins
HUP9802383A2 (hu) Transzkripciós szabályozó szekvenciák és eljárások alkalmazásukra
DE69632827D1 (de) Materialen und methoden im zusammenhang mit bindung und präsentation von substanzen auf zelloberflächen
AU7833494A (en) Enhanced expression in plants using non-translated leader sequences
NO962188D0 (no) Nye apoptose-modulerende proteiner, DNA som koder for proteinene og fremgangsmåter for anvendelse derav
Polstein et al. Light-inducible gene regulation with engineered zinc finger proteins
ATE295888T1 (de) Genfallen-konstrukt zur identifizierung und isolierung von genen
WO2023230570A3 (en) Compositions and methods for modulating genetic drivers
ATE366120T1 (de) Hocheffizientes gewebespezifisches system zur bereitstellung von verbindungen unter verwendung eines streptavidin-protein a fusionsproteins
ATE243741T1 (de) Zufuhr von genprodukten mittels mesangium-zellen
DK1097233T3 (da) Promotor for vækstdifferentieringsfaktor og anvendelser deraf
DE69715672D1 (de) Reporter-zellinie zum nachweis des cytomegalovirus und zur identifizierung von modulatoren der viralen genexpression
DE69233330D1 (de) Verwendung eines Inhibitors von Tau/Neurofilament-Proteinkinase PK40
EP0985045B1 (de) Modulatoren der expression von morphogenen und deren identifizierungsverfahren
WO2001092578A3 (en) Reagents and methods for identifying and modulating expression of genes regulated by retinoids
EP1434483A4 (de) Für nukleinsäuren codierende, miteinander verbundene chromo/fluoreszenzdomänen und verfahren zu ihrer verwendung

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0804565

Country of ref document: EP

REN Ceased due to non-payment of the annual fee