ATE353971T1 - Verbesserte prokariontische proteinenexpression - Google Patents

Verbesserte prokariontische proteinenexpression

Info

Publication number
ATE353971T1
ATE353971T1 AT98917325T AT98917325T ATE353971T1 AT E353971 T1 ATE353971 T1 AT E353971T1 AT 98917325 T AT98917325 T AT 98917325T AT 98917325 T AT98917325 T AT 98917325T AT E353971 T1 ATE353971 T1 AT E353971T1
Authority
AT
Austria
Prior art keywords
procariotic
improved
protein expression
alteration
wpra gene
Prior art date
Application number
AT98917325T
Other languages
English (en)
Inventor
Colin Robert Harwood
Keith Stephenson
Steen Jorgensen
Christina Jensen
Tina Kristensen
Original Assignee
Univ Newcastle Ventures Ltd
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=10811386&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE353971(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Newcastle Ventures Ltd, Novozymes As filed Critical Univ Newcastle Ventures Ltd
Application granted granted Critical
Publication of ATE353971T1 publication Critical patent/ATE353971T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT98917325T 1997-04-26 1998-04-24 Verbesserte prokariontische proteinenexpression ATE353971T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9708452.9A GB9708452D0 (en) 1997-04-26 1997-04-26 Improved prokaryotic expression of protein

Publications (1)

Publication Number Publication Date
ATE353971T1 true ATE353971T1 (de) 2007-03-15

Family

ID=10811386

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98917325T ATE353971T1 (de) 1997-04-26 1998-04-24 Verbesserte prokariontische proteinenexpression

Country Status (9)

Country Link
EP (1) EP0977875B1 (de)
JP (1) JP4312834B2 (de)
CN (1) CN1142280C (de)
AT (1) ATE353971T1 (de)
AU (1) AU7057098A (de)
DE (1) DE69837086T2 (de)
DK (1) DK0977875T3 (de)
GB (1) GB9708452D0 (de)
WO (1) WO1998049328A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100411238B1 (ko) * 2000-08-19 2003-12-18 한국과학기술원 외래 단백질을 안정하게 발현하는 바실러스 속 미생물
US7521204B2 (en) * 2002-04-10 2009-04-21 Novozymes A/S Bacillus host cell
WO2003087149A2 (en) * 2002-04-10 2003-10-23 Novozymes A/S Bacillus licheniformis mutant host cell
US7951556B2 (en) 2004-04-07 2011-05-31 Novozymes A/S Mutated prokaryotic cells with high secretion-levels
JP4485341B2 (ja) * 2004-12-20 2010-06-23 花王株式会社 組換え微生物
US7981659B2 (en) 2005-10-13 2011-07-19 Kao Corporation Bacillus subtilis mutant strain
AR100605A1 (es) 2014-05-27 2016-10-19 Novozymes As Métodos para la producción de lipasas
EP3233893B1 (de) * 2014-12-16 2021-01-20 Danisco US Inc. Erhöhte protein expression
US11624061B2 (en) 2017-04-28 2023-04-11 Agrospheres, Inc. Compositions and methods for enzyme immobilization
BR112019022539A2 (pt) 2017-04-28 2020-06-16 Agrospheres, Inc. Composições e métodos para o encapsulamento e entrega escalonável de agorquímicos
BR112020005803A2 (pt) 2017-09-25 2020-12-01 Agrospheres, Inc. composições e métodos para produção e administração moduláveis de produtos biológicos
CN110592125B (zh) * 2019-09-30 2023-09-01 南京农业大学 一种食品级降解乙醇枯草杆菌重组菌的构建方法
CN113025601B (zh) * 2019-12-25 2024-06-21 上海奥博生物医药股份有限公司 腈水解酶启动子优化表达及应用
CN115948395B (zh) * 2022-08-23 2026-06-19 南京农业大学 一种人工改造的木糖诱导型启动子及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2003078A1 (en) * 1988-11-18 1990-05-18 Biotechnica International, Inc. Protease deletion
US5294542A (en) * 1991-03-19 1994-03-15 Omnigene, Inc. Residual protease-III

Also Published As

Publication number Publication date
GB9708452D0 (en) 1997-06-18
DE69837086T2 (de) 2007-11-15
AU7057098A (en) 1998-11-24
EP0977875A1 (de) 2000-02-09
DE69837086D1 (de) 2007-03-29
JP4312834B2 (ja) 2009-08-12
CN1142280C (zh) 2004-03-17
DK0977875T3 (da) 2007-06-04
JP2001527401A (ja) 2001-12-25
WO1998049328A1 (en) 1998-11-05
CN1253591A (zh) 2000-05-17
EP0977875B1 (de) 2007-02-14

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