ATE367439T1 - Expressionsklonierung in filamentösen pilzen - Google Patents

Expressionsklonierung in filamentösen pilzen

Info

Publication number
ATE367439T1
ATE367439T1 AT98966868T AT98966868T ATE367439T1 AT E367439 T1 ATE367439 T1 AT E367439T1 AT 98966868 T AT98966868 T AT 98966868T AT 98966868 T AT98966868 T AT 98966868T AT E367439 T1 ATE367439 T1 AT E367439T1
Authority
AT
Austria
Prior art keywords
expression cloning
filamentous fungi
interest
dna sequences
proteins
Prior art date
Application number
AT98966868T
Other languages
English (en)
Inventor
Den Brink Johannes Van
Gerard Selten
Den Hombergh Johannes Petr Van
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8229117&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE367439(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of ATE367439T1 publication Critical patent/ATE367439T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Mushroom Cultivation (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)
AT98966868T 1997-12-22 1998-12-22 Expressionsklonierung in filamentösen pilzen ATE367439T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97204079 1997-12-22

Publications (1)

Publication Number Publication Date
ATE367439T1 true ATE367439T1 (de) 2007-08-15

Family

ID=8229117

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98966868T ATE367439T1 (de) 1997-12-22 1998-12-22 Expressionsklonierung in filamentösen pilzen

Country Status (13)

Country Link
US (1) US20020155536A1 (de)
EP (2) EP1854890A1 (de)
JP (3) JP4410413B2 (de)
CN (1) CN1198924C (de)
AT (1) ATE367439T1 (de)
AU (1) AU736111C (de)
BR (1) BR9814404A (de)
CA (1) CA2313445C (de)
DE (1) DE69838106T2 (de)
DK (1) DK1042461T3 (de)
ES (1) ES2287989T3 (de)
PL (1) PL341760A1 (de)
WO (1) WO1999032617A2 (de)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
CN100378218C (zh) 1999-10-01 2008-04-02 诺沃奇梅兹有限公司 喷雾干燥的酶产品
KR20020026456A (ko) 2000-04-13 2002-04-10 에말파브 마크 아론 사상균에서 발현된 ｄｎａ 라이브러리의 고산출량 스크리닝
AU5354401A (en) * 2000-04-13 2001-10-30 Emalfarb, Mark Aaron High-throughput screening of expressed DNA libraries in filamentous fungi
US7514110B1 (en) 2000-09-21 2009-04-07 Basf Aktiengesellschaft Talaromyces xylanases
ATE475324T1 (de) 2000-12-07 2010-08-15 Dsm Ip Assets Bv Mit carboxy-terminal prolin peptide angereicherte proteinhydrolysate
AU2002358179A1 (en) 2001-12-21 2003-07-09 Dsm Ip Assets B.V. New rennets
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
WO2004078919A2 (en) * 2003-02-27 2004-09-16 Midwest Research Institute Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
EP1776461A2 (de) * 2004-04-16 2007-04-25 DSMIP Assets B.V. Pilzpromotoren zur expression eines gens in einer pilzzelle
US9045748B2 (en) 2004-05-27 2015-06-02 Novozymes, Inc. Methods for transforming and expression screening of filamentous fungal cells with a DNA library
EA014853B1 (ru) * 2004-10-15 2011-02-28 ДСМ АйПи АССЕТС Б.В. Амидаза из aspergillus niger и применение амидазы для получения пищевого продукта с пониженным содержанием акриламида
US7888489B2 (en) 2005-01-24 2011-02-15 Dsm Ip Assets B.V. Method for producing a compound of interest in a filamentous fungal cell
JP2008532535A (ja) * 2005-03-18 2008-08-21 ディーエスエム アイピー アセッツ ビー．ブイ． 宿主細胞における改善された発現クローニング法
EP1954812B1 (de) 2005-11-29 2012-11-21 DSM IP Assets B.V. Dna-bindungsstelle eines transkriptionsaktivators mit eignung bei der genexpression
WO2007063133A2 (en) 2005-12-01 2007-06-07 Dsm Ip Assets B.V. Genes useful for the industrial production of citric acid
WO2008000632A1 (en) 2006-06-29 2008-01-03 Dsm Ip Assets B.V. A method for achieving improved polypeptide expression
EP2511372A1 (de) 2006-11-02 2012-10-17 DSM IP Assets B.V. Verbesserte Herstellung von sekretierten Proteinen durch filamentöse Pilze
EP2505651A3 (de) 2006-12-10 2013-01-09 Dyadic International, Inc. Pilzisolate mit reduzierter Proteaseaktivität
EA018840B1 (ru) 2007-02-15 2013-11-29 ДСМ АйПи АССЕТС Б.В. Рекомбинантная клетка-хозяин для получения соединения, представляющего интерес
CA2676686C (en) 2007-02-20 2015-12-15 Dsm Ip Assets B.V. Novel sialidase
US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
CN102741421B (zh) 2009-03-10 2015-03-25 帝斯曼知识产权资产管理有限公司 用于提高多肽产率的方法
DK2406372T3 (da) 2009-03-10 2017-11-27 Dsm Ip Assets Bv Prægastrisk esterase og derivater deraf
EP2421986A1 (de) 2009-04-22 2012-02-29 DSM IP Assets B.V. Verfahren zur herstellung eines interessierenden rekombinanten polypeptids
EP3293264A1 (de) 2009-07-22 2018-03-14 DSM IP Assets B.V. Verbesserte wirtszelle für die herstellung von relevanten produkten
IN2012DN03186A (de) 2009-11-04 2015-09-25 Dsm Ip Assets Bv
EP2531590A2 (de) 2010-02-04 2012-12-12 DSM IP Assets B.V. Verfahren zur herstellung von filamentösen pilzstämmen mit einem sexuellen zyklus und verfahren zur herstellung sexuell gekreuzter, filamentöser pilzstämme
EA201201125A1 (ru) 2010-02-11 2013-03-29 ДСМ АйПи АССЕТС Б.В. Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала
AU2011273351A1 (en) 2010-07-01 2013-01-17 Dsm Ip Assets B.V. A method for the production of a compound of interest
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
US20150197760A1 (en) 2012-06-19 2015-07-16 Dsm Ip Assets B.V. Promoters for expressing a gene in a cell
JP6468561B2 (ja) 2012-07-19 2019-02-13 ディーエスエム アイピー アセッツ ビー．ブイ．Ｄｓｍ Ｉｐ Ａｓｓｅｔｓ Ｂ．Ｖ． ＡｇｓＥ欠損株
PL3686280T3 (pl) 2013-02-04 2022-09-19 Dsm Ip Assets B.V. Polipeptyd degradujący węglowodany i jego zastosowania
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
DK3077517T3 (en) 2013-12-02 2019-02-11 Dsm Ip Assets Bv ISSTRUCTURING PROTEIN
CN105940103B (zh) * 2014-01-31 2021-06-15 株式会社Api 哌可酸4位羟化酶和利用该酶的4-羟基氨基酸的制造法
WO2015177153A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
US10450554B2 (en) 2014-05-19 2019-10-22 Dsm Ip Assets B.V. Proline specific endoprotease
WO2015177152A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
WO2016110453A1 (en) 2015-01-06 2016-07-14 Dsm Ip Assets B.V. A crispr-cas system for a filamentous fungal host cell
AR104205A1 (es) 2015-04-09 2017-07-05 Dsm Ip Assets Bv Fosfolipasa c
WO2016193292A1 (en) 2015-06-02 2016-12-08 Dsm Ip Assets B.V. Use of ice structuring protein afp19 expressed in filamentous fungal strains for preparing food
EP4361240A3 (de) 2015-11-17 2024-07-31 DSM IP Assets B.V. Herstellung eines stabilen bieres
WO2018019948A1 (en) 2016-07-29 2018-02-01 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
ES2784318T3 (es) * 2016-09-20 2020-09-24 Clariant Int Ltd Método para una expresión selectiva independiente de la fuente de carbono de secuencias que codifican una proteína en una célula de hongo filamentoso
US20200199599A1 (en) 2016-09-23 2020-06-25 Dsm Ip Assets B.V. A guide-rna expression system for a host cell
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114941A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114940A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
US20200063166A1 (en) 2017-03-13 2020-02-27 Dsm Ip Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
CN110462044A (zh) 2017-04-06 2019-11-15 帝斯曼知识产权资产管理有限公司 自引导整合构建体(sgic)
WO2019215078A1 (en) 2018-05-07 2019-11-14 Dsm Ip Assets B.V. Process for enzymatic oil degumming
BR112020025589A2 (pt) 2018-06-19 2021-03-23 Dsm Ip Assets B.V. variantes de enzima lipolítica
EP4055170A1 (de) 2019-11-04 2022-09-14 DSM IP Assets B.V. Niedrigvolumentransfektion
JP2021093966A (ja) * 2019-12-18 2021-06-24 本田技研工業株式会社 糸状菌への遺伝子導入方法及びその利用
AU2022297881A1 (en) 2021-06-24 2024-01-18 Fonterra Co-Operative Group Limited Recombinant beta-lactoglobulin
US20250304938A1 (en) 2022-05-16 2025-10-02 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2025012325A1 (en) 2023-07-11 2025-01-16 Dsm Ip Assets B.V. Protein arginine deiminase

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) * 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
DE69032616T2 (de) * 1989-06-16 1999-03-11 Genencor International, Inc., Rochester, N.Y. Dna-sequenzen, vektoren und fusionspolypeptide zur erhöhung der sekretion gewünschter polypeptide von filamentösen fungi
WO1991000920A2 (en) * 1989-07-07 1991-01-24 Unilever N.V. Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
JPH0595787A (ja) * 1991-10-04 1993-04-20 Jozo Shigen Kenkyusho:Kk ホスホグリセリン酸キナ−ゼ遺伝子プロモ−タ−、 同タ−ミネ−タ−または／及びこれらからなる 遺伝子発現用ユニツト、並びに、ホスホグリセリ ン酸キナ−ゼのゲノム遺伝子及びｃＤＮＡ遺伝子
MX9206979A (es) * 1991-12-04 1993-07-01 Novo Nordisk As Metodo de clonacion de proteinas en levaduras
JPH05268964A (ja) * 1992-03-30 1993-10-19 Meiji Milk Prod Co Ltd 糸状菌プロモーター
DE69432543T2 (de) * 1993-07-23 2003-12-24 Dsm N.V., Te Heerlen Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme
JP3638657B2 (ja) * 1995-03-29 2005-04-13 株式会社資生堂 Ｄｎａ修復酵素
CA2219136A1 (en) * 1995-04-24 1996-10-31 Chromaxome Corp. Methods for generating and screening novel metabolic pathways
JP3343567B2 (ja) * 1995-06-29 2002-11-11 大関株式会社 糸状菌のエンハンサーｄｎａ塩基配列およびその用途
DK0873398T3 (da) * 1996-01-10 2005-08-15 Novozymes As Fremgangsmåde til in vivo produktion af et mutantbibliotek i celler

Also Published As

Publication number Publication date
CN1198924C (zh) 2005-04-27
JP2002509698A (ja) 2002-04-02
EP1042461B1 (de) 2007-07-18
CA2313445A1 (en) 1999-07-01
WO1999032617A2 (en) 1999-07-01
JP2005261438A (ja) 2005-09-29
US20020155536A1 (en) 2002-10-24
EP1042461A2 (de) 2000-10-11
AU2515199A (en) 1999-07-12
DK1042461T3 (da) 2007-09-24
JP4410413B2 (ja) 2010-02-03
EP1854890A1 (de) 2007-11-14
CA2313445C (en) 2012-03-20
ES2287989T3 (es) 2007-12-16
BR9814404A (pt) 2000-10-10
JP2010046080A (ja) 2010-03-04
DE69838106D1 (de) 2007-08-30
CN1283227A (zh) 2001-02-07
WO1999032617A3 (en) 1999-09-10
AU736111B2 (en) 2001-07-26
AU736111C (en) 2004-02-19
PL341760A1 (en) 2001-05-07
DE69838106T2 (de) 2008-04-03

Similar Documents

Publication Publication Date Title
ATE367439T1 (de) Expressionsklonierung in filamentösen pilzen
ATE323770T1 (de) Polypeptide mit lysophospholipaseaktivität und nukleinesäuren die sie kodieren
EP2298872A3 (de) Polypeptide mit Lipase-Aktivität und diese kodierende Polynukleotide
WO2001029222A3 (en) Polypeptides having phospholipase b activity and nucleic acids encoding same
WO2002062999A3 (en) Proteins and nucleic acids encoding same
DK0917571T3 (da) Hidtil ukendt agoutirelateret gen
DE60033138D1 (de) Polypeptide mit galaktoseoxidaseaktivität und dafür kodierende nukleisäuren
ATE71660T1 (de) Autonome replikationssequenzen fuer hefestaemme des genus pichia.
DK0894126T3 (da) Alkalisk phosphatase-deficient trådformet svamp
AU7381098A (en) Polypeptides having prolyl pipeptidyl aminopeptidase activity and nucleic acids encoding same
DK1117798T3 (da) Fungal transskriptionel aktivator egnet ved fremgangsmåder til produktion af polypeptider
WO1994018316A3 (en) Apolipoprotein b mrna editing protein compositions and methods
WO2000075350A3 (en) Nucleic acid sequences encoding proteins involved in fatty acid beta-oxidation and methods of use
CA2315274A1 (en) Polypeptides having aminopeptidase activity and nucleic acids encoding same
DE3585953D1 (de) Dna-fragmente, expressionsvektoren, proteine, wirte und verfahren zur herstellung der proteine.
ATE384787T1 (de) Tripeptidyl-aminopeptidase
WO2002059315A3 (en) Human nucleic acids and polypeptides and methods of use thereof
DE69837475D1 (de) Polypeptide mit aminopeptidaseaktivität und dafür kodierende nukleinsäuren
WO1995034651A3 (en) Corticotropin-releasing factor2 receptors
DK1082423T3 (da) Cyclin E2-gener og -proteiner
DE60025467D1 (de) Oxalacetat-hydrolase defiziente fungale wirtszellen
AU2001238064A1 (en) 18477, a human protein kinase and uses therefor
PT977859E (pt) O factor neurotrofico nnt-1
DK1032683T3 (da) Polypeptider med 5-aminolevulinsyre-syntase-aktivitet og nukleinsyrer, der koder for disse
WO2002046408A3 (en) Human proteins, polynucleotides encoding them and methods of using the same

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1042461

Country of ref document: EP