ATE367439T1 - Expressionsklonierung in filamentösen pilzen - Google Patents

Expressionsklonierung in filamentösen pilzen

Info

Publication number
ATE367439T1
ATE367439T1 AT98966868T AT98966868T ATE367439T1 AT E367439 T1 ATE367439 T1 AT E367439T1 AT 98966868 T AT98966868 T AT 98966868T AT 98966868 T AT98966868 T AT 98966868T AT E367439 T1 ATE367439 T1 AT E367439T1
Authority
AT
Austria
Prior art keywords
expression cloning
filamentous fungi
interest
dna sequences
proteins
Prior art date
Application number
AT98966868T
Other languages
English (en)
Inventor
Den Brink Johannes Van
Gerard Selten
Den Hombergh Johannes Petr Van
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8229117&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE367439(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of ATE367439T1 publication Critical patent/ATE367439T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Mushroom Cultivation (AREA)
  • Peptides Or Proteins (AREA)
AT98966868T 1997-12-22 1998-12-22 Expressionsklonierung in filamentösen pilzen ATE367439T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP97204079 1997-12-22

Publications (1)

Publication Number Publication Date
ATE367439T1 true ATE367439T1 (de) 2007-08-15

Family

ID=8229117

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98966868T ATE367439T1 (de) 1997-12-22 1998-12-22 Expressionsklonierung in filamentösen pilzen

Country Status (13)

Country Link
US (1) US20020155536A1 (de)
EP (2) EP1042461B1 (de)
JP (3) JP4410413B2 (de)
CN (1) CN1198924C (de)
AT (1) ATE367439T1 (de)
AU (1) AU736111C (de)
BR (1) BR9814404A (de)
CA (1) CA2313445C (de)
DE (1) DE69838106T2 (de)
DK (1) DK1042461T3 (de)
ES (1) ES2287989T3 (de)
PL (1) PL341760A1 (de)
WO (1) WO1999032617A2 (de)

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EP1224272B1 (de) 1999-10-01 2005-12-07 Novozymes A/S Sprühgetrocknetes enzymprodukt
AU5354401A (en) * 2000-04-13 2001-10-30 Emalfarb, Mark Aaron High-throughput screening of expressed DNA libraries in filamentous fungi
US7122330B2 (en) 2000-04-13 2006-10-17 Mark Aaron Emalfarb High-throughput screening of expressed DNA libraries in filamentous fungi
CA2422748C (en) 2000-09-21 2011-08-09 Dsm N.V. Talaromyces xylanases
DK1339837T3 (da) 2000-12-07 2008-06-09 Dsm Ip Assets Bv Prolyl-endoprotease fra Aspergillus Niger
US7404977B2 (en) 2001-12-21 2008-07-29 Dsm Ip Assets B.V. Rennets
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
AU2003219956A1 (en) * 2003-02-27 2004-09-28 Midwest Research Institute Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
CA2560205A1 (en) 2004-04-16 2005-10-27 Dsm Ip Assets B.V. Fungal promoters for expressing a gene in a fungal cell
WO2005121351A2 (en) * 2004-05-27 2005-12-22 Novozymes, Inc. Methods for transforming and expression screening of filamentous fungal cells with a dna library
BRPI0516523A (pt) * 2004-10-15 2008-09-09 Dsm Ip Assets Bv processo para a produção de alimento
DK2410048T3 (en) 2005-01-24 2016-11-28 Dsm Ip Assets Bv A process for the preparation of a compound of interest in a filamentous fungal cell
US20090209007A1 (en) * 2005-03-18 2009-08-20 Roelf Bernhard Meima Method of Expression Cloning in a Host Cell
EA014783B1 (ru) 2005-11-29 2011-02-28 ДСМ АйПи АССЕТС Б.В. Связывающий днк-сайт активатора транскрипции, пригодный для экспрессии генов
US8637280B2 (en) 2005-12-01 2014-01-28 Adcuram Nutrition Holding Gmbh Genes useful for the industrial production of citric acid
CA2657975A1 (en) 2006-06-29 2008-01-03 Dsm Ip Assets B.V. A method for achieving improved polypeptide expression
WO2008053018A2 (en) 2006-11-02 2008-05-08 Dsm Ip Assets B.V. Improved production of secreted proteins by filamentous fungi
EP2505651A3 (de) 2006-12-10 2013-01-09 Dyadic International, Inc. Pilzisolate mit reduzierter Proteaseaktivität
EP2631295A3 (de) 2007-02-15 2014-01-01 DSM IP Assets B.V. Rekombinante Hostzelle zur Herstellung einer Verbindung von Interesse
CA2676686C (en) 2007-02-20 2015-12-15 Dsm Ip Assets B.V. Novel sialidase
WO2009033071A2 (en) 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
WO2010102982A1 (en) 2009-03-10 2010-09-16 Dsm Ip Assets B.V. Method for improving the yield of a polypeptide
US8703463B2 (en) 2009-03-10 2014-04-22 Dsm Ip Assets B.V. Pregastric esterase and derivatives thereof
WO2010121933A1 (en) 2009-04-22 2010-10-28 Dsm Ip Assets B.V. Process for the production of a recombinant polypeptide of interest
WO2011009700A1 (en) 2009-07-22 2011-01-27 Dsm Ip Assets B.V. Improved host cell for the production of a compound of interest
WO2011054899A1 (en) 2009-11-04 2011-05-12 Dsm Ip Assets B.V. Talaromyces transformants
US20130011876A1 (en) 2010-02-04 2013-01-10 Dsm Ip Assetts, B.V. Process for preparing filamentous fungal strains having a sexual cycle and a process for preparing sexually crossed filamentous fungal strains
CA2789301A1 (en) 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Host cell capable of producing enzymes useful for degradation of lignocellulosic material
CA2803222A1 (en) 2010-07-01 2012-01-05 Dsm Ip Assets B.V. A method for the production of a compound of interest
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
US20150197760A1 (en) 2012-06-19 2015-07-16 Dsm Ip Assets B.V. Promoters for expressing a gene in a cell
EP3327112B1 (de) 2012-07-19 2020-02-12 DSM IP Assets B.V. Agse-armer stamm
EP3686280B1 (de) 2013-02-04 2022-06-22 DSM IP Assets B.V. Kohlenhydratabbauendes polypeptid und verwendungen davon
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
EP3077517B1 (de) 2013-12-02 2018-10-17 DSM IP Assets B.V. Eisstrukturierungsprotein
US9969988B2 (en) * 2014-01-31 2018-05-15 Api Corporation Pipecolinic acid 4-hydroxylase and method for producing 4-hydroxy amino acid using same
WO2015177152A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
WO2015177153A1 (en) 2014-05-19 2015-11-26 Dsm Ip Assets B.V. Proline-specific endoprotease
MX375798B (es) 2014-05-19 2025-03-07 Dsm Ip Assets Bv Endoproteasa específica para prolina.
DK3242950T3 (da) 2015-01-06 2021-12-20 Dsm Ip Assets Bv Crispr-cas-system til en trådformet svampeværtscelle
AR104205A1 (es) 2015-04-09 2017-07-05 Dsm Ip Assets Bv Fosfolipasa c
AU2016273208B2 (en) 2015-06-02 2021-01-21 Dsm Ip Assets B.V. Use of ice structuring protein AFP19 expressed in filamentous fungal strains for preparing food
EP4361240A3 (de) 2015-11-17 2024-07-31 DSM IP Assets B.V. Herstellung eines stabilen bieres
WO2018019948A1 (en) 2016-07-29 2018-02-01 Dsm Ip Assets B.V. Polypeptides having cellulolytic enhancing activity and uses thereof
RS60238B1 (sr) * 2016-09-20 2020-06-30 Clariant Int Ltd Postupak za selektivnu ekspresiju, nezavisnu od izvora ugljenika, sekvenci koje kodiraju proteine u ćeliji filamentozne gljive
FI3516056T3 (fi) 2016-09-23 2025-02-28 Dsm Ip Assets Bv Opas-rna:n ilmentämisjärjestelmä isäntäsolulle
WO2018114940A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
US10918113B2 (en) 2016-12-21 2021-02-16 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114938A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
WO2018114912A1 (en) 2016-12-21 2018-06-28 Dsm Ip Assets B.V. Lipolytic enzyme variants
EP3596199A1 (de) 2017-03-13 2020-01-22 DSM IP Assets B.V. Transkriptioneller regulator-defizienz-stamm mit zweikernigem zink-cluster
US20200032252A1 (en) 2017-04-06 2020-01-30 Dsm Ip Assets B.V. Self-guiding integration construct (sgic)
WO2019215078A1 (en) 2018-05-07 2019-11-14 Dsm Ip Assets B.V. Process for enzymatic oil degumming
BR112020025589A2 (pt) 2018-06-19 2021-03-23 Dsm Ip Assets B.V. variantes de enzima lipolítica
WO2021089452A1 (en) 2019-11-04 2021-05-14 Dsm Ip Assets B.V. Low volume transfection
JP2021093966A (ja) * 2019-12-18 2021-06-24 本田技研工業株式会社 糸状菌への遺伝子導入方法及びその利用
AU2022297881A1 (en) 2021-06-24 2024-01-18 Fonterra Co-Operative Group Limited Recombinant beta-lactoglobulin
EP4525626A1 (de) 2022-05-16 2025-03-26 DSM IP Assets B.V. Varianten eines lipolytischen enzyms
EP4743572A1 (de) 2023-07-11 2026-05-20 DSM IP Assets B.V. Proteinarginindeiminase

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WO1990015860A1 (en) * 1989-06-16 1990-12-27 Genencor, Inc. Dna sequences, vectors, and fusion polypeptides to increase secretion of desired polypeptides from filamentous fungi
ES2109238T3 (es) * 1989-07-07 1998-01-16 Unilever Nv Procedimiento para la preparacion de una proteina mediante un hongo transformado por integracion multicopia de un vector de expresion.
JPH0595787A (ja) * 1991-10-04 1993-04-20 Jozo Shigen Kenkyusho:Kk ホスホグリセリン酸キナ−ゼ遺伝子プロモ−タ−、 同タ−ミネ−タ−または／及びこれらからなる 遺伝子発現用ユニツト、並びに、ホスホグリセリ ン酸キナ−ゼのゲノム遺伝子及びｃＤＮＡ遺伝子
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Also Published As

Publication number Publication date
PL341760A1 (en) 2001-05-07
JP2010046080A (ja) 2010-03-04
CN1283227A (zh) 2001-02-07
DE69838106T2 (de) 2008-04-03
EP1042461A2 (de) 2000-10-11
DE69838106D1 (de) 2007-08-30
AU2515199A (en) 1999-07-12
CA2313445C (en) 2012-03-20
BR9814404A (pt) 2000-10-10
US20020155536A1 (en) 2002-10-24
WO1999032617A2 (en) 1999-07-01
CN1198924C (zh) 2005-04-27
AU736111C (en) 2004-02-19
WO1999032617A3 (en) 1999-09-10
ES2287989T3 (es) 2007-12-16
EP1854890A1 (de) 2007-11-14
DK1042461T3 (da) 2007-09-24
JP2002509698A (ja) 2002-04-02
CA2313445A1 (en) 1999-07-01
JP2005261438A (ja) 2005-09-29
JP4410413B2 (ja) 2010-02-03
EP1042461B1 (de) 2007-07-18
AU736111B2 (en) 2001-07-26

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