ATE418602T1 - Mutierte luciferase mit verbesserter thermostabilität - Google Patents

Mutierte luciferase mit verbesserter thermostabilität

Info

Publication number
ATE418602T1
ATE418602T1 AT04000812T AT04000812T ATE418602T1 AT E418602 T1 ATE418602 T1 AT E418602T1 AT 04000812 T AT04000812 T AT 04000812T AT 04000812 T AT04000812 T AT 04000812T AT E418602 T1 ATE418602 T1 AT E418602T1
Authority
AT
Austria
Prior art keywords
amino acid
acid residue
luciferase
photinus pyralis
residue corresponding
Prior art date
Application number
AT04000812T
Other languages
English (en)
Inventor
David James Squirrell
Melenie Jane Murphy
Rachel Louise Leslie
Christopher Robin Lowe
Laurence Carlo Tisi
James Augustus Henry Murray
Peter John White
Original Assignee
3M Innovative Properties Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 3M Innovative Properties Co filed Critical 3M Innovative Properties Co
Application granted granted Critical
Publication of ATE418602T1 publication Critical patent/ATE418602T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Luminescent Compositions (AREA)
  • Lubricants (AREA)
AT04000812T 1998-10-28 1999-10-26 Mutierte luciferase mit verbesserter thermostabilität ATE418602T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB9823468.5A GB9823468D0 (en) 1998-10-28 1998-10-28 Novel enzyme

Publications (1)

Publication Number Publication Date
ATE418602T1 true ATE418602T1 (de) 2009-01-15

Family

ID=10841350

Family Applications (2)

Application Number Title Priority Date Filing Date
AT99950990T ATE419335T1 (de) 1998-10-28 1999-10-26 Mutierte luciferase mit verbesserter thermostabilität
AT04000812T ATE418602T1 (de) 1998-10-28 1999-10-26 Mutierte luciferase mit verbesserter thermostabilität

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT99950990T ATE419335T1 (de) 1998-10-28 1999-10-26 Mutierte luciferase mit verbesserter thermostabilität

Country Status (11)

Country Link
US (4) US7906298B1 (de)
EP (9) EP2366779B1 (de)
JP (3) JP5185479B2 (de)
AT (2) ATE419335T1 (de)
AU (5) AU770722B2 (de)
CA (1) CA2342300C (de)
DE (2) DE69940208D1 (de)
DK (2) DK1479763T3 (de)
ES (2) ES2319941T3 (de)
GB (2) GB9823468D0 (de)
WO (1) WO2000024878A2 (de)

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US8669103B2 (en) 2010-11-02 2014-03-11 Promega Corporation Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
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EP2776573A1 (de) * 2011-11-11 2014-09-17 Promega Corporation Mutante protease-biosensoren mit verbesserten erkennungseigenschaften
AU2013204332B2 (en) 2012-04-16 2015-07-16 Commonwealth Scientific And Industrial Research Organisation Methods and systems for detecting an analyte or classifying a sample
CN111004835B (zh) 2013-02-22 2023-12-29 普洛麦格公司 用于荧光素酶和核苷磷酸的发光检测的稳定制剂
US9927430B2 (en) 2014-01-29 2018-03-27 Promega Corporation Pro-substrates for live cell applications
WO2015116867A1 (en) 2014-01-29 2015-08-06 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
WO2016040788A1 (en) 2014-09-11 2016-03-17 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US9677117B2 (en) 2014-10-08 2017-06-13 Promega Corporation Bioluminescent succinate detection assay
EP3056902B1 (de) 2015-02-16 2020-04-01 Universite De Bordeaux Neuartige spannungsabhängige Ionenkanalfusionen und Verwendungsverfahren dafür
US11261478B2 (en) * 2016-10-13 2022-03-01 Promega Corporation Thermal stable luciferase with improved resistance to inhibition by luciferin break-down products
AU2017358067B2 (en) 2016-11-14 2024-04-18 PPB Technology Pty Ltd Protease sensor molecules
JP6349003B1 (ja) 2017-03-17 2018-06-27 東洋ビーネット株式会社 耐熱性ルシフェラーゼ
CA3071994A1 (en) 2017-08-08 2019-02-14 Commonwealth Scientific And Industrial Research Organisation Carbohydrate sensors
KR20200071724A (ko) 2017-10-18 2020-06-19 유니버시떼 드 몬트리얼 G-단백질 활성화의 평가를 위한 시스템 및 방법
JP7638057B2 (ja) 2018-05-22 2025-03-03 Ｕｂｅマシナリー株式会社 ダイカストマシン
WO2021162123A1 (ja) 2020-02-14 2021-08-19 キッコーマン株式会社 試料中のａｔｐ、並びにａｍｐ及び／又はａｄｐを測定するための液体組成物
EP3882632A1 (de) 2020-03-18 2021-09-22 Universite De Bordeaux Neuartige ionenleitende kanalfusionuntereinheiten und verfahren zur verwendung davon
EP4317844A4 (de) * 2021-03-31 2025-01-29 Daikin Industries, Ltd. Kältekreislaufvorrichtung
EP4560025A1 (de) 2022-07-22 2025-05-28 Kikkoman Corporation Verfahren und kit zum nachweis von mikroorganismen oder zellen oder nachweis von mikroorganismen oder zellverwandten substanzen
CN120457210A (zh) 2023-01-27 2025-08-08 龟甲万株式会社 萤光素酶突变体

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Also Published As

Publication number Publication date
DK1479763T3 (da) 2009-04-06
US20140170686A1 (en) 2014-06-19
AU2004202276A1 (en) 2004-06-17
JP2012161325A (ja) 2012-08-30
EP1479763B1 (de) 2008-12-24
US7906298B1 (en) 2011-03-15
DK1124944T3 (da) 2009-04-14
EP1124944A2 (de) 2001-08-22
AU2004202277A1 (en) 2004-06-17
AU770722B2 (en) 2004-02-26
US8652794B2 (en) 2014-02-18
JP5185479B2 (ja) 2013-04-17
AU2004200277B2 (en) 2005-11-24
GB2345913B (en) 2004-03-17
EP2071023B1 (de) 2016-04-27
EP2366777A1 (de) 2011-09-21
AU2004202277B2 (en) 2008-05-01
EP2071023A3 (de) 2009-10-14
DE69940172D1 (de) 2009-02-05
ES2319520T3 (es) 2009-05-08
EP2366778A1 (de) 2011-09-21
AU6357399A (en) 2000-05-15
GB2345913A (en) 2000-07-26
EP2071023A2 (de) 2009-06-17
ES2319941T3 (es) 2009-05-14
CA2342300A1 (en) 2000-05-04
WO2000024878A2 (en) 2000-05-04
US20110177540A1 (en) 2011-07-21
GB9823468D0 (en) 1998-12-23
EP2366780B1 (de) 2018-06-13
GB9925162D0 (en) 1999-12-22
JP2003518912A (ja) 2003-06-17
CA2342300C (en) 2009-08-11
JP5951325B2 (ja) 2016-07-13
EP2366777B1 (de) 2018-07-25
EP2366780A1 (de) 2011-09-21
JP2011120605A (ja) 2011-06-23
EP2366779A1 (de) 2011-09-21
AU2008203449A1 (en) 2008-08-28
EP1479763A2 (de) 2004-11-24
EP1479763A3 (de) 2005-05-25
WO2000024878A3 (en) 2000-08-03
EP2368977B1 (de) 2018-06-27
ATE419335T1 (de) 2009-01-15
EP2368976A1 (de) 2011-09-28
EP2071023A8 (de) 2010-06-02
US20140170687A1 (en) 2014-06-19
AU2004200277A1 (en) 2004-02-19
EP1124944B1 (de) 2008-12-31
EP2366778B1 (de) 2012-08-22
EP2368977A1 (de) 2011-09-28
AU2004202276B2 (en) 2008-05-01
EP2366779B1 (de) 2018-07-11
DE69940208D1 (de) 2009-02-12

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