ATE462716T1 - Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen - Google Patents
Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungenInfo
- Publication number
- ATE462716T1 ATE462716T1 AT04796067T AT04796067T ATE462716T1 AT E462716 T1 ATE462716 T1 AT E462716T1 AT 04796067 T AT04796067 T AT 04796067T AT 04796067 T AT04796067 T AT 04796067T AT E462716 T1 ATE462716 T1 AT E462716T1
- Authority
- AT
- Austria
- Prior art keywords
- functional group
- branched alkyl
- target protein
- proteins
- chromatography
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 230000002209 hydrophobic effect Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000004587 chromatography analysis Methods 0.000 title 1
- 230000003993 interaction Effects 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 125000000217 alkyl group Chemical group 0.000 abstract 3
- 125000000524 functional group Chemical group 0.000 abstract 3
- 239000003463 adsorbent Substances 0.000 abstract 2
- 125000004432 carbon atom Chemical group C* 0.000 abstract 2
- 238000004113 cell culture Methods 0.000 abstract 2
- 239000000356 contaminant Substances 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 125000001183 hydrocarbyl group Chemical group 0.000 abstract 1
- 239000012266 salt solution Substances 0.000 abstract 1
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51448603P | 2003-10-24 | 2003-10-24 | |
| PCT/US2004/035007 WO2005042569A1 (en) | 2003-10-24 | 2004-10-22 | Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE462716T1 true ATE462716T1 (de) | 2010-04-15 |
Family
ID=34549339
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04796067T ATE462716T1 (de) | 2003-10-24 | 2004-10-22 | Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7427659B2 (de) |
| EP (1) | EP1687328B1 (de) |
| AT (1) | ATE462716T1 (de) |
| AU (1) | AU2004285928B2 (de) |
| CA (1) | CA2542951C (de) |
| DE (1) | DE602004026343D1 (de) |
| ES (1) | ES2342539T3 (de) |
| WO (1) | WO2005042569A1 (de) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1380290A1 (de) | 2002-07-09 | 2004-01-14 | Universitair Medisch Centrum Utrecht | Cross-Beta-Strukturweg und seine therapeutische Relevanz |
| US7427659B2 (en) * | 2003-10-24 | 2008-09-23 | Amgen Inc. | Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction |
| BRPI0608584B8 (pt) | 2005-03-11 | 2021-05-25 | Wyeth Corp | métodos de recuperar um produto purificado |
| EP2386861A3 (de) | 2005-07-13 | 2012-07-18 | Crossbeta Biosciences B.V. | Cross-Beta-Struktur-bindende Verbindungen |
| US8114832B2 (en) * | 2005-07-13 | 2012-02-14 | Crossbeta Biosciences B.V. | Method for detecting and/or removing a protein comprising a cross-beta structure from a pharmaceutical composition |
| US20070015133A1 (en) * | 2005-07-13 | 2007-01-18 | Umc Utrecht Holding B.V. | Method for detecting and/or removing protein and/or peptide comprising a cross-beta structure from an aqueous solution comprising a protein |
| US7566401B2 (en) * | 2005-10-03 | 2009-07-28 | Kelm Mark A | Process for separating and isolating xanthines, individual polar protic monomers, and polar protic oligomers |
| AR058568A1 (es) | 2005-12-20 | 2008-02-13 | Bristol Myers Squibb Co | Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo |
| PT2253644E (pt) * | 2005-12-20 | 2014-01-23 | Bristol Myers Squibb Co | Composições e métodos para a produção de uma composição |
| WO2009102509A2 (en) | 2008-01-10 | 2009-08-20 | Research Development Foundation | Vaccines and diagnostics for the ehrlichioses |
| WO2009126603A1 (en) * | 2008-04-08 | 2009-10-15 | Bio-Rad Laboratories, Inc. | Chromatography purification of antibodies |
| NZ590257A (en) * | 2008-07-18 | 2012-08-31 | Grifols Therapeutics Inc | Method of preparing alpha-1 proteinase inhibitor |
| JP5956331B2 (ja) * | 2009-06-16 | 2016-07-27 | ジェンザイム・コーポレーション | 組換えaavベクターの改良された精製方法 |
| SG176963A1 (en) | 2009-06-22 | 2012-02-28 | Amgen Inc | Refolding proteins using a chemically controlled redox state |
| LT3660032T (lt) | 2009-06-25 | 2026-02-25 | Amgen Inc. | Ne žinduolių sistemoje ekspresuotų baltymų surišamojo gryninimo būdai |
| JP2013509188A (ja) * | 2009-10-30 | 2013-03-14 | アボット・ラボラトリーズ | Sorf構築物および複数の遺伝子発現 |
| EP2652491B1 (de) | 2010-12-15 | 2017-11-29 | Baxalta GmbH | Eluatsammlung mit leitfähigkeitsgradient |
| WO2012149197A2 (en) | 2011-04-27 | 2012-11-01 | Abbott Laboratories | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| JP6292718B2 (ja) | 2011-07-01 | 2018-03-14 | エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft | 凝集ポリペプチドから単量体ポリペプチドを分離するための方法 |
| JP6280499B2 (ja) * | 2011-07-08 | 2018-02-14 | メルク・シャープ・アンド・ドーム・コーポレーションMerck Sharp & Dohme Corp. | Fc融合タンパク質の精製方法 |
| ES2651483T3 (es) * | 2011-08-17 | 2018-01-26 | Ares Trading S.A. | Método para preparar la forma activa de la proteína de fusión TNRF-Fc |
| SG10201701224UA (en) | 2012-03-12 | 2017-04-27 | Merck Patent Gmbh | Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| US9150645B2 (en) | 2012-04-20 | 2015-10-06 | Abbvie, Inc. | Cell culture methods to reduce acidic species |
| US9181572B2 (en) | 2012-04-20 | 2015-11-10 | Abbvie, Inc. | Methods to modulate lysine variant distribution |
| US9249182B2 (en) | 2012-05-24 | 2016-02-02 | Abbvie, Inc. | Purification of antibodies using hydrophobic interaction chromatography |
| HK1211981A1 (en) | 2012-09-02 | 2016-06-03 | Abbvie Inc. | Methods to control protein heterogeneity |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| SG11201507230PA (en) | 2013-03-12 | 2015-10-29 | Abbvie Inc | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| US10023608B1 (en) | 2013-03-13 | 2018-07-17 | Amgen Inc. | Protein purification methods to remove impurities |
| WO2014159579A1 (en) | 2013-03-14 | 2014-10-02 | Abbvie Inc. | MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE |
| WO2014151878A2 (en) | 2013-03-14 | 2014-09-25 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosacharides |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| US9994609B2 (en) * | 2013-03-15 | 2018-06-12 | Biogen Ma Inc. | Hydrophobic interaction protein chromatography under no-salt conditions |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| US9951101B2 (en) | 2013-12-12 | 2018-04-24 | Emd Millipore Corporation | Protein separations using an acrylamide containing filter |
| CA2942134C (en) * | 2014-03-04 | 2023-08-15 | Merck Patent Gmbh | Robust antibody purification |
| AR102198A1 (es) | 2014-10-09 | 2017-02-08 | Regeneron Pharma | Proceso para reducir partículas subvisibles en una formulación farmacéutica |
| AU2017312785B2 (en) | 2016-08-16 | 2024-10-10 | Regeneron Pharmaceuticals, Inc. | Methods for quantitating individual antibodies from a mixture |
| KR102759512B1 (ko) | 2016-10-25 | 2025-02-03 | 리제너론 파아마슈티컬스, 인크. | 크로마토그래피 데이터 분석을 위한 방법 및 시스템 |
| MY199833A (en) | 2017-08-17 | 2023-11-24 | Just Evotec Biologics Inc | Method of purifying glycosylated protein from host cell galectins and other contaminants |
| WO2019060062A1 (en) | 2017-09-19 | 2019-03-28 | Regeneron Pharmaceuticals Inc. | METHODS OF REDUCING PARTICLE FORMATION AND COMPOSITIONS FORMED THEREFROM |
| TWI919355B (zh) | 2018-07-02 | 2026-03-21 | 美商里珍納龍藥品有限公司 | 自混合物製備多肽之系統及方法 |
| EA202192422A1 (ru) | 2019-03-05 | 2021-12-09 | Ридженерон Фармасьютикалз, Инк. | Человеческий сывороточный альбумин в составах |
| WO2021207657A1 (en) | 2020-04-09 | 2021-10-14 | Cytomx Therapeutics, Inc. | Compositions containing activatable antibodies |
| EP4267717A1 (de) | 2020-12-22 | 2023-11-01 | Amgen Inc. | Zellkulturverfahren |
| EP4518839A2 (de) | 2022-05-02 | 2025-03-12 | Regeneron Pharmaceuticals, Inc. | Verfahren zur reduzierung der lipaseaktivität |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0449299A (ja) | 1990-06-14 | 1992-02-18 | Tosoh Corp | 免疫グロブリンフラグメントの精製法 |
| US5463029A (en) * | 1992-11-23 | 1995-10-31 | Immunex Corporation | Purification of fusion proteins comprising GM-CSF and IL-3 |
| US7214787B1 (en) * | 1993-09-21 | 2007-05-08 | United States Of America As Represented By The Secretary Of The Army | Recombinant vaccine against botulinum neurotoxin |
| US5429746A (en) * | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| US5468847A (en) * | 1994-03-10 | 1995-11-21 | Minnesota Mining And Manufacturing Company | Method of isolating and purifying a biomacromolecule |
| SE503424C2 (sv) * | 1994-11-14 | 1996-06-10 | Pharmacia Ab | Process för rening av rekombinant koagulationsfaktor VIII |
| US5641870A (en) * | 1995-04-20 | 1997-06-24 | Genentech, Inc. | Low pH hydrophobic interaction chromatography for antibody purification |
| GB9907553D0 (en) | 1999-04-01 | 1999-05-26 | Cantab Pharma Res | Purification of biological preparations |
| EP1681299B1 (de) * | 2000-03-27 | 2009-11-11 | Genetics Institute, LLC | Verfahren zur Reinigung stark anionischer Proteine |
| US7427659B2 (en) * | 2003-10-24 | 2008-09-23 | Amgen Inc. | Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction |
-
2004
- 2004-10-22 US US10/970,860 patent/US7427659B2/en not_active Expired - Lifetime
- 2004-10-22 DE DE602004026343T patent/DE602004026343D1/de not_active Expired - Lifetime
- 2004-10-22 WO PCT/US2004/035007 patent/WO2005042569A1/en not_active Ceased
- 2004-10-22 ES ES04796067T patent/ES2342539T3/es not_active Expired - Lifetime
- 2004-10-22 AT AT04796067T patent/ATE462716T1/de not_active IP Right Cessation
- 2004-10-22 EP EP04796067A patent/EP1687328B1/de not_active Expired - Lifetime
- 2004-10-22 AU AU2004285928A patent/AU2004285928B2/en not_active Expired
- 2004-10-22 CA CA2542951A patent/CA2542951C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US20050136521A1 (en) | 2005-06-23 |
| CA2542951A1 (en) | 2005-05-12 |
| CA2542951C (en) | 2016-08-30 |
| US7427659B2 (en) | 2008-09-23 |
| ES2342539T3 (es) | 2010-07-08 |
| EP1687328A1 (de) | 2006-08-09 |
| AU2004285928A1 (en) | 2005-05-12 |
| EP1687328A4 (de) | 2006-11-15 |
| AU2004285928B2 (en) | 2012-02-02 |
| WO2005042569A1 (en) | 2005-05-12 |
| EP1687328B1 (de) | 2010-03-31 |
| DE602004026343D1 (de) | 2010-05-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |