ATE462716T1 - Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen - Google Patents
Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungenInfo
- Publication number
- ATE462716T1 ATE462716T1 AT04796067T AT04796067T ATE462716T1 AT E462716 T1 ATE462716 T1 AT E462716T1 AT 04796067 T AT04796067 T AT 04796067T AT 04796067 T AT04796067 T AT 04796067T AT E462716 T1 ATE462716 T1 AT E462716T1
- Authority
- AT
- Austria
- Prior art keywords
- functional group
- branched alkyl
- target protein
- proteins
- chromatography
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 230000002209 hydrophobic effect Effects 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000004587 chromatography analysis Methods 0.000 title 1
- 230000003993 interaction Effects 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
- 125000000217 alkyl group Chemical group 0.000 abstract 3
- 125000000524 functional group Chemical group 0.000 abstract 3
- 239000003463 adsorbent Substances 0.000 abstract 2
- 125000004432 carbon atom Chemical group C* 0.000 abstract 2
- 238000004113 cell culture Methods 0.000 abstract 2
- 239000000356 contaminant Substances 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 229910052799 carbon Inorganic materials 0.000 abstract 1
- 125000001183 hydrocarbyl group Chemical group 0.000 abstract 1
- 239000012266 salt solution Substances 0.000 abstract 1
- 125000000999 tert-butyl group Chemical group [H]C([H])([H])C(*)(C([H])([H])[H])C([H])([H])[H] 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51448603P | 2003-10-24 | 2003-10-24 | |
| PCT/US2004/035007 WO2005042569A1 (en) | 2003-10-24 | 2004-10-22 | Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE462716T1 true ATE462716T1 (de) | 2010-04-15 |
Family
ID=34549339
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04796067T ATE462716T1 (de) | 2003-10-24 | 2004-10-22 | Verfahren zur aufreinigung von proteinen in einer durchflussfraktion aus der chromatographie mit hydrophoben wechselwirkungen |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7427659B2 (de) |
| EP (1) | EP1687328B1 (de) |
| AT (1) | ATE462716T1 (de) |
| AU (1) | AU2004285928B2 (de) |
| CA (1) | CA2542951C (de) |
| DE (1) | DE602004026343D1 (de) |
| ES (1) | ES2342539T3 (de) |
| WO (1) | WO2005042569A1 (de) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1380290A1 (de) | 2002-07-09 | 2004-01-14 | Universitair Medisch Centrum Utrecht | Cross-Beta-Strukturweg und seine therapeutische Relevanz |
| US7427659B2 (en) * | 2003-10-24 | 2008-09-23 | Amgen Inc. | Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction |
| EP1869065B1 (de) | 2005-03-11 | 2020-05-06 | Wyeth LLC | Verfahren zur chromatographie mit schwacher partition |
| US8114832B2 (en) * | 2005-07-13 | 2012-02-14 | Crossbeta Biosciences B.V. | Method for detecting and/or removing a protein comprising a cross-beta structure from a pharmaceutical composition |
| AU2006267176A1 (en) | 2005-07-13 | 2007-01-18 | Crossbeta Biosciences B.V. | Cross-beta structure binding compounds |
| US20070015133A1 (en) * | 2005-07-13 | 2007-01-18 | Umc Utrecht Holding B.V. | Method for detecting and/or removing protein and/or peptide comprising a cross-beta structure from an aqueous solution comprising a protein |
| US7566401B2 (en) * | 2005-10-03 | 2009-07-28 | Kelm Mark A | Process for separating and isolating xanthines, individual polar protic monomers, and polar protic oligomers |
| AR058568A1 (es) | 2005-12-20 | 2008-02-13 | Bristol Myers Squibb Co | Metodos para producir una composicion con moleculas ctla4-ig a partir de un medio de cultivo |
| DK1969007T3 (da) * | 2005-12-20 | 2013-11-25 | Bristol Myers Squibb Co | Sammensætninger og fremgangsmåder til fremstilling af en sammensætning |
| BRPI0906429B1 (pt) | 2008-01-10 | 2021-08-03 | Research Development Foundation | Método de identificação de uma infecção por e. chaffeensis em um indivíduo, uso de um ou mais polipeptídeo sintético e kit |
| WO2009126603A1 (en) * | 2008-04-08 | 2009-10-15 | Bio-Rad Laboratories, Inc. | Chromatography purification of antibodies |
| EP2321336A1 (de) * | 2008-07-18 | 2011-05-18 | Talecris Biotherapeutics, Inc. | Verfahren zur herstellung eines alpha-1-proteinaseinhibitors |
| KR20180000341A (ko) * | 2009-06-16 | 2018-01-02 | 젠자임 코포레이션 | 재조합 aav 벡터에 대한 개선된 정제 방법 |
| EP3366692A1 (de) | 2009-06-22 | 2018-08-29 | Amgen, Inc | Proteinneufaltung mit einem chemisch gesteuerten redoxzustand |
| AU2010266093B2 (en) | 2009-06-25 | 2013-06-27 | Amgen Inc. | Capture purification processes for proteins expressed in a non-mammalian system |
| WO2011053699A1 (en) | 2009-10-30 | 2011-05-05 | Abbott Laboratories | Sorf constructs and multiple gene expression |
| SG191186A1 (en) | 2010-12-15 | 2013-07-31 | Baxter Int | Eluate collection using conductivity gradient |
| US9062106B2 (en) | 2011-04-27 | 2015-06-23 | Abbvie Inc. | Methods for controlling the galactosylation profile of recombinantly-expressed proteins |
| SG10201605397PA (en) | 2011-07-01 | 2016-08-30 | Hoffmann La Roche | Method For Separation Of Monomeric Polypeptides From Aggregated Polypeptides |
| DK2729482T3 (en) | 2011-07-08 | 2018-05-07 | Merck Sharp & Dohme | PROCEDURE FOR CLEANING FC-FUSION PROTEIN |
| KR101454316B1 (ko) * | 2011-08-17 | 2014-10-27 | 한화케미칼 주식회사 | 활성형 TNFR-Fc 융합 단백질을 제조하는 방법 |
| SG10201701224UA (en) | 2012-03-12 | 2017-04-27 | Merck Patent Gmbh | Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode |
| WO2013158279A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Protein purification methods to reduce acidic species |
| US9067990B2 (en) | 2013-03-14 | 2015-06-30 | Abbvie, Inc. | Protein purification using displacement chromatography |
| WO2013158273A1 (en) | 2012-04-20 | 2013-10-24 | Abbvie Inc. | Methods to modulate c-terminal lysine variant distribution |
| WO2013176754A1 (en) * | 2012-05-24 | 2013-11-28 | Abbvie Inc. | Novel purification of antibodies using hydrophobic interaction chromatography |
| HK1211981A1 (en) | 2012-09-02 | 2016-06-03 | Abbvie Inc. | Methods to control protein heterogeneity |
| US9512214B2 (en) | 2012-09-02 | 2016-12-06 | Abbvie, Inc. | Methods to control protein heterogeneity |
| HK1207960A1 (en) | 2013-03-12 | 2016-02-19 | Abbvie Inc. | Human antibodies that bind human tnf-alpha and methods of preparing the same |
| US10023608B1 (en) | 2013-03-13 | 2018-07-17 | Amgen Inc. | Protein purification methods to remove impurities |
| WO2014159579A1 (en) | 2013-03-14 | 2014-10-02 | Abbvie Inc. | MUTATED ANTI-TNFα ANTIBODIES AND METHODS OF THEIR USE |
| US9017687B1 (en) | 2013-10-18 | 2015-04-28 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same using displacement chromatography |
| US9499614B2 (en) | 2013-03-14 | 2016-11-22 | Abbvie Inc. | Methods for modulating protein glycosylation profiles of recombinant protein therapeutics using monosaccharides and oligosaccharides |
| JP6592426B2 (ja) | 2013-03-15 | 2019-10-16 | バイオジェン・エムエイ・インコーポレイテッドBiogen MA Inc. | 無塩条件下で疎水性相互作用クロマトグラフィーを使用するタンパク質精製 |
| US9598667B2 (en) | 2013-10-04 | 2017-03-21 | Abbvie Inc. | Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins |
| US9085618B2 (en) | 2013-10-18 | 2015-07-21 | Abbvie, Inc. | Low acidic species compositions and methods for producing and using the same |
| US8946395B1 (en) | 2013-10-18 | 2015-02-03 | Abbvie Inc. | Purification of proteins using hydrophobic interaction chromatography |
| US9181337B2 (en) | 2013-10-18 | 2015-11-10 | Abbvie, Inc. | Modulated lysine variant species compositions and methods for producing and using the same |
| US20150139988A1 (en) | 2013-11-15 | 2015-05-21 | Abbvie, Inc. | Glycoengineered binding protein compositions |
| JP6326499B2 (ja) | 2013-12-12 | 2018-05-16 | イー・エム・デイー・ミリポア・コーポレイシヨン | アクリルアミド含有フィルターを使用したタンパク質分離 |
| WO2015131978A1 (en) * | 2014-03-04 | 2015-09-11 | Merck Patent Gmbh | Robust antibody purification |
| AR102198A1 (es) | 2014-10-09 | 2017-02-08 | Regeneron Pharma | Proceso para reducir partículas subvisibles en una formulación farmacéutica |
| US11020686B2 (en) | 2016-08-16 | 2021-06-01 | Regeneron Pharmaceuticals, Inc. | Methods for quantitating individual antibodies from a mixture |
| JP7102401B2 (ja) | 2016-10-25 | 2022-07-19 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | クロマトグラフィーデータ解析のための方法およびシステム |
| EP3668985B1 (de) | 2017-08-17 | 2021-06-16 | Just-Evotec Biologics, Inc. | Verfahren zur reinigung von glykosyliertem protein aus wirtszellen-galectinen und anderen verunreinigungen |
| CA3073935A1 (en) | 2017-09-19 | 2019-03-28 | Regeneron Pharmaceuticals, Inc. | Methods of reducing particle formation and compositions formed thereby |
| TWI853823B (zh) | 2018-07-02 | 2024-09-01 | 美商里珍納龍藥品有限公司 | 自混合物製備多肽之系統及方法 |
| EA202192422A1 (ru) | 2019-03-05 | 2021-12-09 | Ридженерон Фармасьютикалз, Инк. | Человеческий сывороточный альбумин в составах |
| JP2023521384A (ja) | 2020-04-09 | 2023-05-24 | シートムエックス セラピューティクス,インコーポレイテッド | 活性化可能抗体を含む組成物 |
| KR20230124578A (ko) | 2020-12-22 | 2023-08-25 | 암젠 인크 | 세포 배양 방법 |
| AU2023265659A1 (en) | 2022-05-02 | 2024-12-12 | Regeneron Pharmaceuticals, Inc. | Methods for reducing lipase activity |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0449299A (ja) * | 1990-06-14 | 1992-02-18 | Tosoh Corp | 免疫グロブリンフラグメントの精製法 |
| US5463029A (en) * | 1992-11-23 | 1995-10-31 | Immunex Corporation | Purification of fusion proteins comprising GM-CSF and IL-3 |
| US7214787B1 (en) * | 1993-09-21 | 2007-05-08 | United States Of America As Represented By The Secretary Of The Army | Recombinant vaccine against botulinum neurotoxin |
| US5429746A (en) | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| US5468847A (en) * | 1994-03-10 | 1995-11-21 | Minnesota Mining And Manufacturing Company | Method of isolating and purifying a biomacromolecule |
| SE503424C2 (sv) * | 1994-11-14 | 1996-06-10 | Pharmacia Ab | Process för rening av rekombinant koagulationsfaktor VIII |
| US5641870A (en) * | 1995-04-20 | 1997-06-24 | Genentech, Inc. | Low pH hydrophobic interaction chromatography for antibody purification |
| GB9907553D0 (en) * | 1999-04-01 | 1999-05-26 | Cantab Pharma Res | Purification of biological preparations |
| AU4951501A (en) | 2000-03-27 | 2001-10-08 | Genetics Inst | Methods for purifying highly anionic proteins |
| US7427659B2 (en) * | 2003-10-24 | 2008-09-23 | Amgen Inc. | Process for purifying proteins in a hydrophobic interaction chromatography flow-through fraction |
-
2004
- 2004-10-22 US US10/970,860 patent/US7427659B2/en not_active Expired - Lifetime
- 2004-10-22 AU AU2004285928A patent/AU2004285928B2/en not_active Expired
- 2004-10-22 DE DE602004026343T patent/DE602004026343D1/de not_active Expired - Lifetime
- 2004-10-22 CA CA2542951A patent/CA2542951C/en not_active Expired - Lifetime
- 2004-10-22 EP EP04796067A patent/EP1687328B1/de not_active Expired - Lifetime
- 2004-10-22 AT AT04796067T patent/ATE462716T1/de not_active IP Right Cessation
- 2004-10-22 WO PCT/US2004/035007 patent/WO2005042569A1/en not_active Ceased
- 2004-10-22 ES ES04796067T patent/ES2342539T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US7427659B2 (en) | 2008-09-23 |
| DE602004026343D1 (de) | 2010-05-12 |
| ES2342539T3 (es) | 2010-07-08 |
| AU2004285928B2 (en) | 2012-02-02 |
| AU2004285928A1 (en) | 2005-05-12 |
| EP1687328B1 (de) | 2010-03-31 |
| CA2542951C (en) | 2016-08-30 |
| US20050136521A1 (en) | 2005-06-23 |
| EP1687328A4 (de) | 2006-11-15 |
| EP1687328A1 (de) | 2006-08-09 |
| WO2005042569A1 (en) | 2005-05-12 |
| CA2542951A1 (en) | 2005-05-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |