ATE525087T1 - Verfahren zur aufreinigung von antikörpern - Google Patents

Verfahren zur aufreinigung von antikörpern

Info

Publication number
ATE525087T1
ATE525087T1 AT06753791T AT06753791T ATE525087T1 AT E525087 T1 ATE525087 T1 AT E525087T1 AT 06753791 T AT06753791 T AT 06753791T AT 06753791 T AT06753791 T AT 06753791T AT E525087 T1 ATE525087 T1 AT E525087T1
Authority
AT
Austria
Prior art keywords
ion exchange
purifying antibodies
immunoglobulin
purification
single step
Prior art date
Application number
AT06753791T
Other languages
English (en)
Inventor
Roberto Falkenstein
Burkard Kolb
Maria Smida
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34936911&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE525087(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE525087T1 publication Critical patent/ATE525087T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/96—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation using ion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oncology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT06753791T 2005-05-25 2006-05-23 Verfahren zur aufreinigung von antikörpern ATE525087T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05011302 2005-05-25
PCT/EP2006/004863 WO2006125599A2 (en) 2005-05-25 2006-05-23 Method for the purification of antibodies

Publications (1)

Publication Number Publication Date
ATE525087T1 true ATE525087T1 (de) 2011-10-15

Family

ID=34936911

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06753791T ATE525087T1 (de) 2005-05-25 2006-05-23 Verfahren zur aufreinigung von antikörpern

Country Status (18)

Country Link
US (3) US20090098660A1 (de)
EP (2) EP1888636B2 (de)
JP (1) JP4852602B2 (de)
KR (1) KR100967778B1 (de)
CN (1) CN101180317B (de)
AT (1) ATE525087T1 (de)
AU (1) AU2006251307B2 (de)
BR (1) BRPI0610201B8 (de)
CA (1) CA2607375C (de)
DK (1) DK1888636T4 (de)
ES (2) ES2373402T5 (de)
FI (1) FI1888636T4 (de)
IL (2) IL186338A (de)
MX (1) MX2007014269A (de)
NO (1) NO345362B1 (de)
PL (1) PL1888636T5 (de)
TW (2) TWI391399B (de)
WO (1) WO2006125599A2 (de)

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RU2009148891A (ru) 2007-06-01 2011-07-20 Ф.Хоффманн-Ля Рош Аг (Ch) Очистка иммуноглобулина
AR067537A1 (es) * 2007-07-17 2009-10-14 Hoffmann La Roche Purificacion de polipeptidos pegilados
CL2008002054A1 (es) * 2007-07-17 2009-05-29 Hoffmann La Roche Metodo para la regeneracion de una columna de cromatografia de intercambio cationico despues de la elusion de eritropoyetina monopeguilada y metodo para obtener una eritropoyetina monopeguilada, incorporando el metodo de regeneracion de la columna de intercambio cationico.
JP5529869B2 (ja) * 2008-08-14 2014-06-25 メルク・シャープ・アンド・ドーム・コーポレーション プロテインａアフィニティークロマトグラフィーを用いる抗体の精製方法
BR112012020964B8 (pt) 2010-03-10 2021-08-24 Hoffmann La Roche método para produzir uma imunoglobulina da subclasse igg1 ou igg4
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JP6292718B2 (ja) 2011-07-01 2018-03-14 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 凝集ポリペプチドから単量体ポリペプチドを分離するための方法
US10481164B2 (en) * 2012-03-26 2019-11-19 Amgen Inc. Method for using light scattering in real time to directly monitor and control impurity removal in purification processes
KR20160054597A (ko) * 2013-09-17 2016-05-16 가부시키가이샤 가네카 신규 항체 정제 방법 및 그로부터 얻어지는 항체, 및 양이온 교환기를 사용한 신규 항체 정제법 및 그로부터 얻어지는 항체
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CN111068361B (zh) * 2018-10-22 2022-02-11 中国石油化工股份有限公司 一种己内酰胺离子交换装置及其再生方法
CN110066314B (zh) * 2019-04-01 2023-10-27 上海药明生物技术有限公司 一种高效提高多聚体分离分辨率的亲和纯化工艺
TW202128264A (zh) * 2019-09-25 2021-08-01 瑞士商赫孚孟拉羅股份公司 使用經預測之溶離緩衝鹽濃度的陽離子層析法
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CN115728433B (zh) * 2021-08-27 2024-11-15 联邦生物科技(珠海横琴)有限公司 IgG4型单克隆抗体电荷异质性的离子交换色谱检测方法
CN116295834B (zh) * 2022-01-05 2026-03-10 上海迈晋生物医药科技有限公司 一种生物制品在层析工艺中的表观稳定性的在线监测方法
CN120939820A (zh) * 2025-07-31 2025-11-14 浙江安各洛生物技术有限公司 一种梯度混合方法及其配套的梯度混合装置

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Also Published As

Publication number Publication date
EP1888636B2 (de) 2022-03-30
TW200716669A (en) 2007-05-01
US20140243508A1 (en) 2014-08-28
ES2389641T5 (es) 2021-06-10
EP1888636B1 (de) 2011-09-21
AU2006251307B2 (en) 2011-08-04
DK1888636T4 (da) 2022-07-18
WO2006125599A3 (en) 2007-06-21
ES2373402T3 (es) 2012-02-03
EP1888636A2 (de) 2008-02-20
CN101180317B (zh) 2013-06-19
CA2607375C (en) 2014-01-21
NO345362B1 (no) 2020-12-28
ES2389641T3 (es) 2012-10-30
CA2607375A1 (en) 2006-11-30
KR100967778B1 (ko) 2010-07-05
US11034754B2 (en) 2021-06-15
PL1888636T3 (pl) 2012-02-29
TWI391399B (zh) 2013-04-01
BRPI0610201A2 (pt) 2010-06-01
JP2008542218A (ja) 2008-11-27
AU2006251307A1 (en) 2006-11-30
FI1888636T4 (fi) 2023-01-31
WO2006125599A2 (en) 2006-11-30
US9631007B2 (en) 2017-04-25
TWI320788B (en) 2010-02-21
BRPI0610201B8 (pt) 2021-05-25
IL206993A0 (en) 2012-01-01
KR20080017322A (ko) 2008-02-26
ES2373402T5 (es) 2022-09-14
EP2261252A3 (de) 2011-03-23
IL186338A0 (en) 2008-01-20
US20090098660A1 (en) 2009-04-16
IL186338A (en) 2011-01-31
BRPI0610201B1 (pt) 2020-12-29
CA2607375F (en) 2006-11-30
NO20075091L (no) 2008-01-23
CN101180317A (zh) 2008-05-14
EP2261252B1 (de) 2012-07-25
TW200946543A (en) 2009-11-16
JP4852602B2 (ja) 2012-01-11
EP2261252A2 (de) 2010-12-15
DK1888636T3 (da) 2011-11-14
MX2007014269A (es) 2008-01-24
EP2261252B2 (de) 2020-10-21
PL1888636T5 (pl) 2023-03-06
US20170183394A1 (en) 2017-06-29

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