BRPI0417252A - processo de purificação de beta interferon humano - Google Patents

processo de purificação de beta interferon humano

Info

Publication number
BRPI0417252A
BRPI0417252A BRPI0417252-3A BRPI0417252A BRPI0417252A BR PI0417252 A BRPI0417252 A BR PI0417252A BR PI0417252 A BRPI0417252 A BR PI0417252A BR PI0417252 A BRPI0417252 A BR PI0417252A
Authority
BR
Brazil
Prior art keywords
buffer solution
interferon beta
washing
propylene glycol
purification process
Prior art date
Application number
BRPI0417252-3A
Other languages
English (en)
Inventor
Ji Sook Park
Jong Sang Chung
Min Ji Baek
Jee Won Ahn
Ki Wan Kim
Hyung Ki Park
Dong Eok Lee
Myung Suk Oh
Original Assignee
Cj Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cj Corp filed Critical Cj Corp
Publication of BRPI0417252A publication Critical patent/BRPI0417252A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

"PROCESSO DE PURIFICAçãO DE BETA INTERFERON HUMANO". é proporcionado um processo de purificação do beta interferon humano a partir de uma cultura que contém beta interferon humano recombinante realizando cromatografia por afinidade e cromatografia de permuta catiónica, em que a cromatografia por afinidade inclui: adsorver a cultura que contém beta interferon numa coluna de cromatografia por afinidade equilibrada, seguida de lavagem com uma solução tampão equilibrada; lavar a coluna com uma solução tampão de lavagem A de pH 6,5-7,5 contendo 30-60% em peso de propilenoglicol e uma solução tampão de lavagem B de pH 6,5-7,5 contendo 30-60% em peso de propilenoglicol e NaCI 1-2M; e eluir uma fração contendo beta interferon com uma solução tampão de pH 6,5-7,5 contendo 40-60% em peso de propileno e NaCI 1-2M.
BRPI0417252-3A 2003-12-04 2004-12-04 processo de purificação de beta interferon humano BRPI0417252A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2003-0087553A KR100524872B1 (ko) 2003-12-04 2003-12-04 인터페론 베타의 정제방법
PCT/KR2004/003180 WO2005054289A1 (en) 2003-12-04 2004-12-04 Process for purifying interferon beta

Publications (1)

Publication Number Publication Date
BRPI0417252A true BRPI0417252A (pt) 2007-03-06

Family

ID=36647278

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0417252-3A BRPI0417252A (pt) 2003-12-04 2004-12-04 processo de purificação de beta interferon humano

Country Status (14)

Country Link
US (1) US7901670B2 (pt)
EP (1) EP1694703B1 (pt)
JP (1) JP2007530448A (pt)
KR (1) KR100524872B1 (pt)
CN (1) CN1902224B (pt)
AT (1) ATE522617T1 (pt)
AU (1) AU2004295266B2 (pt)
BR (1) BRPI0417252A (pt)
CA (1) CA2548165C (pt)
ES (1) ES2370019T3 (pt)
MX (1) MXPA06006356A (pt)
PL (1) PL1694703T3 (pt)
RU (1) RU2346002C2 (pt)
WO (1) WO2005054289A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG155183A1 (en) * 2005-08-26 2009-09-30 Ares Trading Sa Process for the preparation of glycosylated interferon beta
DE102007050165B4 (de) * 2007-10-19 2010-06-17 Stiftung Tierärztliche Hochschule Hannover Stabilisierte Lösung, Verfahren zu deren Herstellung sowie deren Verwendung und Arzneimittel in Form einer stabilisierten Lösung
KR101113495B1 (ko) * 2008-01-23 2012-02-29 한미홀딩스 주식회사 인간 인터페론 베타의 정제방법
RU2392282C1 (ru) * 2008-10-06 2010-06-20 Учреждение Российской академии наук Институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова РАН СПОСОБ ОЧИСТКИ РЕКОМБИНАНТНОГО ИНТЕРФЕРОНА β-1b
EP2542574B1 (en) * 2010-03-04 2017-08-09 Pfenex Inc. Method for producing soluble recombinant interferon protein without denaturing
CN102219848B (zh) * 2011-05-25 2014-01-22 浙江海正药业股份有限公司 重组人干扰素β-1a的纯化方法
DK2771022T3 (da) 2011-10-11 2020-09-28 Viela Bio Inc Cd40l-specifikke tn3-afledte skeletter (scaffolds) og fremgangsmåder til anvendelse deraf
CN107428817B (zh) 2015-03-12 2022-07-12 免疫医疗有限责任公司 纯化白蛋白融合蛋白的方法
EA027609B9 (ru) * 2015-04-29 2017-11-30 Тютор С.А.С.И.Ф.И.А. Способ получения интерферона бета-1альфа и фармацевтическая композиция, содержащая интерферон бета-1альфа

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5564799A (en) 1978-11-07 1980-05-15 Toray Ind Inc Multi-stage concentration and purification of interferon originated from human fibroblast
US4289689A (en) 1980-03-14 1981-09-15 Hoffmann-La Roche Inc. Preparation of homogeneous human fibroblast interferon
US4278661A (en) * 1979-10-12 1981-07-14 E. I. Du Pont De Nemours And Company Purification of interferon
NL7907791A (nl) 1979-10-23 1981-04-27 Stichting Rega V Z W Werkwijze voor het zuiveren van interferon.
JPS58201794A (ja) 1982-05-17 1983-11-24 Toray Ind Inc ヒトインターフェロンβの濃縮精製法
US4966843A (en) 1982-11-01 1990-10-30 Cetus Corporation Expression of interferon genes in Chinese hamster ovary cells
US4483849A (en) * 1983-01-07 1984-11-20 Carter William A Stabilization and purification of interferon with propylene glycol, resulting in a non-toxic product
US4658017A (en) * 1984-02-14 1987-04-14 Health Research, Inc. (Roswell Park Division) Method for the large scale purification of human fibroblast interferon
US4808523A (en) * 1984-11-07 1989-02-28 Yeda Research And Development Co., Ltd. Constitutive production of human IFN-β1 by mammalian cells transformed by the IFN-β1 gene fused to an SV40 early promoter
IT1222427B (it) 1987-07-31 1990-09-05 Sclavo Spa Procedimento per la purificazione di interferone
IT1240612B (it) * 1990-03-16 1993-12-17 Sclavo Spa Procedimento di purificazione del b-interferone umano ricombinante
EP0551535A1 (en) 1992-01-13 1993-07-21 SCLAVO S.p.A. Process for the purification of recombinant human beta interferon, beta interferon thus purified, and pharmaceutical compositions which contain them
US7144574B2 (en) 1999-08-27 2006-12-05 Maxygen Aps Interferon β variants and conjugates

Also Published As

Publication number Publication date
EP1694703A4 (en) 2008-08-27
AU2004295266A1 (en) 2005-06-16
PL1694703T3 (pl) 2011-12-30
CA2548165A1 (en) 2005-06-16
KR100524872B1 (ko) 2005-11-01
CA2548165C (en) 2011-10-04
EP1694703B1 (en) 2011-08-31
US20070123697A1 (en) 2007-05-31
ES2370019T3 (es) 2011-12-12
US7901670B2 (en) 2011-03-08
KR20050054210A (ko) 2005-06-10
JP2007530448A (ja) 2007-11-01
AU2004295266B2 (en) 2009-01-08
RU2006123543A (ru) 2008-01-10
CN1902224B (zh) 2012-05-30
ATE522617T1 (de) 2011-09-15
RU2346002C2 (ru) 2009-02-10
CN1902224A (zh) 2007-01-24
WO2005054289A1 (en) 2005-06-16
MXPA06006356A (es) 2006-08-23
EP1694703A1 (en) 2006-08-30

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Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: CJ CHEILJEDANG CORPORATION (KR)

Free format text: TRANSFERIDO DE: CJ CORPORATION

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 11A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2343 DE 01-12-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.