BRPI0819526A2 - "fragmento de ácido nucléico isolado, construção recombinante, célula de planta e método para reduzir a expressão de uma sequência alvo" - Google Patents

"fragmento de ácido nucléico isolado, construção recombinante, célula de planta e método para reduzir a expressão de uma sequência alvo"

Info

Publication number
BRPI0819526A2
BRPI0819526A2 BRPI0819526-9A2A BRPI0819526A BRPI0819526A2 BR PI0819526 A2 BRPI0819526 A2 BR PI0819526A2 BR PI0819526 A BRPI0819526 A BR PI0819526A BR PI0819526 A2 BRPI0819526 A2 BR PI0819526A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
target sequence
plant cell
acid fragment
reducing expression
Prior art date
Application number
BRPI0819526-9A2A
Other languages
English (en)
Inventor
Brian Mcgonigle
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of BRPI0819526A2 publication Critical patent/BRPI0819526A2/pt
Publication of BRPI0819526B1 publication Critical patent/BRPI0819526B1/pt
Publication of BRPI0819526B8 publication Critical patent/BRPI0819526B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
BRPI0819526A 2007-12-18 2008-12-17 Sequência de ácido nucleico de precursor de mirna artificial, construção recombinante e método para reduzir a expressão de uma sequência alvo BRPI0819526B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US1451207P 2007-12-18 2007-12-18
US61/014,512 2007-12-18
PCT/US2008/087082 WO2009079532A2 (en) 2007-12-18 2008-12-17 Down-regulation of gene expression using artificial micrornas

Publications (3)

Publication Number Publication Date
BRPI0819526A2 true BRPI0819526A2 (pt) 2015-03-10
BRPI0819526B1 BRPI0819526B1 (pt) 2018-03-06
BRPI0819526B8 BRPI0819526B8 (pt) 2022-12-06

Family

ID=40427383

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0819526A BRPI0819526B8 (pt) 2007-12-18 2008-12-17 Sequência de ácido nucleico de precursor de mirna artificial, construção recombinante e método para reduzir a expressão de uma sequência alvo

Country Status (7)

Country Link
US (3) US8937217B2 (pt)
EP (4) EP2458010B1 (pt)
BR (1) BRPI0819526B8 (pt)
CA (3) CA2902723C (pt)
ES (2) ES2421031T3 (pt)
PL (1) PL2261363T3 (pt)
WO (1) WO2009079532A2 (pt)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8937217B2 (en) 2007-12-18 2015-01-20 E. I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs
EP2542682A2 (en) 2010-03-03 2013-01-09 E.I. Du Pont De Nemours And Company Plant seeds with altered storage compound levels, related constructs and methods involving genes encoding oxidoreductase motif polypeptides
BR112012027150A2 (pt) 2010-04-23 2015-09-15 Du Pont método para regular a expressão em uma célula vegetal, interruptor gênico, planta transgênica, célula vegetal transgênica ou semente transgênica e polinucleotídeo recombinante
US9062317B2 (en) 2011-05-09 2015-06-23 E I Du Pont De Nemours And Company Methods and compositions for silencing gene families using artificial microRNAs
EP2771467A1 (en) 2011-10-28 2014-09-03 E. I. Du Pont de Nemours and Company Methods and compositions for silencing genes using artificial micrornas
CA2860220C (en) 2011-12-22 2021-07-13 E. I. Du Pont De Nemours And Company Use of the soybean or medicago truncatula sucrose synthase promoter to increase plant seed oil content
BR112014018009A2 (pt) 2012-01-23 2018-06-26 Du Pont sequência de ácido nucleico isolada, construção recombinante, célula vegetal, método para reduzir a expressão de pelo menos um gene biossintético de ácido graxo vegetal, método para reduzir a expressão de dois ou mais genes biossintéticos de ácido graxo vegetal e planta ou semente transgênica
EP2806730A4 (en) 2012-01-23 2015-12-23 Linnaeus Plant Sciences Inc MODIFICATION OF THE FATTY ACID PROFILE OF CAMELINE OIL
EP2839007B1 (en) 2012-04-19 2017-12-27 Temasek Life Sciences Laboratory Limited Methods for increasing cotton fiber length
US10837014B2 (en) 2012-05-16 2020-11-17 Translate Bio Ma, Inc. Compositions and methods for modulating SMN gene family expression
CN104583402A (zh) 2012-05-16 2015-04-29 Rana医疗有限公司 用于调节mecp2表达的组合物和方法
EA201492120A1 (ru) 2012-05-16 2015-10-30 Рана Терапьютикс, Инк. Композиции и способы для модулирования экспрессии atp2a2
CN104540946A (zh) 2012-05-16 2015-04-22 Rana医疗有限公司 用于调节utrn表达的组合物和方法
US10174315B2 (en) 2012-05-16 2019-01-08 The General Hospital Corporation Compositions and methods for modulating hemoglobin gene family expression
JP2015523854A (ja) 2012-05-16 2015-08-20 ラナ セラピューティクス インコーポレイテッド Ｓｍｎ遺伝子ファミリー発現を調節するための組成物及び方法
WO2014164775A1 (en) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Methods and compositions to improve the spread of chemical signals in plants
BR112015023268A2 (pt) 2013-03-13 2018-05-02 Du Pont método para reduzir a expressão, construto de dna, método de obtenção de célula vegetal, método de obtenção de planta transgênica ou uma parte de planta
CA3200166A1 (en) 2013-03-14 2014-09-25 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
WO2015017510A1 (en) 2013-07-31 2015-02-05 E. I. Du Pont De Nemours And Company Modification of soybean seed composition to enhance feed, food and other industrial applications of soybean products
AR100159A1 (es) 2014-04-22 2016-09-14 Du Pont Genes de anhidrasa carbónica plastídica para el incremento de aceite en semillas con expresión incrementada de dgat
US10640773B2 (en) 2014-04-30 2020-05-05 Fondazione Edmund Mach Plant sRNA extract or plant miRNA for use as an immunosuppressive agent
WO2016044092A1 (en) 2014-09-17 2016-03-24 Pioneer Hi Bred International Inc Compositions and methods to control insect pests
WO2016064347A1 (en) 2014-10-22 2016-04-28 Temasek Life Sciences Laboratory Limited Terpene synthases from ylang ylang (cananga odorata var. fruticosa)
WO2016130943A1 (en) 2015-02-13 2016-08-18 Rana Therapeutics, Inc. Hybrid oligonucleotides and uses thereof
US10647995B2 (en) 2015-06-16 2020-05-12 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
EP3368089B1 (en) 2015-10-26 2025-11-05 Translate Bio Ma, Inc. Nanoparticle formulations for delivery of nucleic acid complexes
EP3472323A1 (en) 2016-06-16 2019-04-24 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
US20210292778A1 (en) 2016-07-12 2021-09-23 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
MX2019009371A (es) 2017-02-08 2019-09-23 Pionner Hi Bred Int Inc Combinaciones insecticidas de proteinas insecticidas derivadas de plantas y metodos para su uso.
US20200165626A1 (en) 2017-10-13 2020-05-28 Pioneer Hi-Bred International, Inc. Virus-induced gene silencing technology for insect control in maize

Family Cites Families (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
KR920701453A (ko) 1989-03-17 1992-08-11 미리엄 디. 멕코나헤이 유전자발현의 외부조절
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
ES2187497T3 (es) 1990-04-12 2003-06-16 Syngenta Participations Ag Promotores preferentemente en tejidos.
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
JPH06510187A (ja) 1991-08-27 1994-11-17 ノバルティス　アクチエンゲゼルシャフト 同翅類昆虫に対する殺虫性質を有したタンパク質及び植物保護におけるそれらの用法
ATE276373T1 (de) 1991-10-04 2004-10-15 Univ North Carolina State Pathogenresistente transgene pflanzen
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
US5428148A (en) 1992-04-24 1995-06-27 Beckman Instruments, Inc. N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
AU670316B2 (en) 1992-07-27 1996-07-11 Pioneer Hi-Bred International, Inc. An improved method of (agrobacterium)-mediated transformation of cultured soybean cells
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5470353A (en) 1993-10-20 1995-11-28 Hollister Incorporated Post-operative thermal blanket
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5750868A (en) 1994-12-08 1998-05-12 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
AU3495297A (en) 1996-07-08 1998-02-02 Pioneer Hi-Bred International, Inc. Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
BR9810807A (pt) 1997-07-22 2005-12-06 Pioneer Hi Bred Int Genes controlando metabolismo de filato em plantas e seus usos
AU754376B2 (en) 1998-02-26 2002-11-14 Pioneer Hi-Bred International, Inc. Family of maize PR-1 genes and promoters
ES2270227T3 (es) 1998-02-26 2007-04-01 Pioneer Hi-Bred International, Inc. Promotor met-1 del maiz.
SE9801630L (sv) 1998-05-11 1999-11-12 Ericsson Telefon Ab L M Förfarande och anordning vid ett kommunikationsnät
CA2332628C (en) 1998-08-20 2005-04-12 Pioneer Hi-Bred International, Inc. Seed-preferred promoters
AU5788299A (en) 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from (end) genes
CA2408326A1 (en) 2000-06-23 2002-01-03 E.I. Dupont De Nemours And Company Recombinant constructs and their use in reducing gene expression
US7323560B2 (en) 2000-07-17 2008-01-29 E.I. Du Pont De Nemours And Company Plastidic phosphoglucomutase genes
AU2002247060A1 (en) 2001-01-12 2002-08-06 E.I. Dupont De Nemours And Company Novel inositol polyphosphate kinase genes and uses thereof
AU2002331897A1 (en) 2001-09-27 2003-04-07 Pioneer Hi-Bred International, Inc. Phytate polynucleotides and methods of use
ATE522130T1 (de) 2002-07-19 2011-09-15 Univ South Carolina Zusammensetzungen und verfahren zur modulation der genexpression in pflanzen
WO2005035769A2 (en) 2003-10-09 2005-04-21 E. I. Du Pont De Nemours And Company Gene silencing by using micro-rna molecules
US20060135758A1 (en) * 2004-08-31 2006-06-22 Kunsheng Wu Soybean polymorphisms and methods of genotyping
WO2006073727A2 (en) 2004-12-21 2006-07-13 Monsanto Technology, Llc Recombinant dna constructs and methods for controlling gene expression
US20060200878A1 (en) * 2004-12-21 2006-09-07 Linda Lutfiyya Recombinant DNA constructs and methods for controlling gene expression
US8314290B2 (en) * 2004-12-21 2012-11-20 Monsanto Technology Llc Temporal regulation of gene expression by MicroRNAs
KR100918296B1 (ko) 2007-12-14 2009-09-18 한국전자통신연구원 착신 전환 서비스 제공 시스템 및 방법
US8937217B2 (en) 2007-12-18 2015-01-20 E. I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs

Also Published As

Publication number Publication date
CA2902723A1 (en) 2009-06-25
WO2009079532A3 (en) 2009-09-17
US20150087069A1 (en) 2015-03-26
EP2458010B1 (en) 2013-05-01
EP2270183A3 (en) 2011-03-16
EP2261363A3 (en) 2011-03-16
CA2902723C (en) 2016-10-18
EP2261363A2 (en) 2010-12-15
CA2902719A1 (en) 2009-06-25
EP2261363B1 (en) 2016-04-20
PL2261363T3 (pl) 2016-12-30
EP2270183A2 (en) 2011-01-05
ES2388489T3 (es) 2012-10-15
BRPI0819526B8 (pt) 2022-12-06
US20110091975A1 (en) 2011-04-21
CA2709404C (en) 2016-10-11
US20090155910A1 (en) 2009-06-18
US9546374B2 (en) 2017-01-17
EP2229447A2 (en) 2010-09-22
EP2261363A8 (en) 2011-02-16
CA2709404A1 (en) 2009-06-25
WO2009079532A2 (en) 2009-06-25
CA2902719C (en) 2016-10-18
ES2421031T3 (es) 2013-08-28
US8937217B2 (en) 2015-01-20
BRPI0819526B1 (pt) 2018-03-06
EP2458010A1 (en) 2012-05-30
EP2270183B1 (en) 2012-06-13

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