CA2005478A1 - Methode de solubilisation et de naturation de la somatotrophine - Google Patents

Methode de solubilisation et de naturation de la somatotrophine

Info

Publication number
CA2005478A1
CA2005478A1 CA2005478A CA2005478A CA2005478A1 CA 2005478 A1 CA2005478 A1 CA 2005478A1 CA 2005478 A CA2005478 A CA 2005478A CA 2005478 A CA2005478 A CA 2005478A CA 2005478 A1 CA2005478 A1 CA 2005478A1
Authority
CA
Canada
Prior art keywords
naturation
somatotropin
solubilization
denaturants
eliminates
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA2005478A
Other languages
English (en)
Other versions
CA2005478C (fr
Inventor
Kevin M. Mccoy
Robert A. Frost
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wyeth Holdings LLC
Original Assignee
American Cyanamid Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by American Cyanamid Co filed Critical American Cyanamid Co
Publication of CA2005478A1 publication Critical patent/CA2005478A1/fr
Application granted granted Critical
Publication of CA2005478C publication Critical patent/CA2005478C/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Endocrinology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Medicines Containing Plant Substances (AREA)
CA002005478A 1988-12-16 1989-12-14 Methode de solubilisation et de naturation de la somatotrophine Expired - Fee Related CA2005478C (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/285,477 US5064943A (en) 1988-12-16 1988-12-16 Method for solubilization and naturation of somatotropin
US07/285,477 1988-12-16

Publications (2)

Publication Number Publication Date
CA2005478A1 true CA2005478A1 (fr) 1990-06-16
CA2005478C CA2005478C (fr) 2000-01-04

Family

ID=23094405

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002005478A Expired - Fee Related CA2005478C (fr) 1988-12-16 1989-12-14 Methode de solubilisation et de naturation de la somatotrophine

Country Status (18)

Country Link
US (1) US5064943A (fr)
EP (1) EP0373325B1 (fr)
JP (1) JPH02222697A (fr)
KR (1) KR0148681B1 (fr)
AT (1) ATE136309T1 (fr)
AU (1) AU628443B2 (fr)
CA (1) CA2005478C (fr)
DE (1) DE68926153T2 (fr)
DK (1) DK174662B1 (fr)
ES (1) ES2087065T3 (fr)
FI (1) FI102179B (fr)
GR (1) GR3019691T3 (fr)
HU (1) HU213572B (fr)
IE (1) IE74848B1 (fr)
IL (1) IL92135A (fr)
NZ (1) NZ231681A (fr)
PT (1) PT92305B (fr)
ZA (1) ZA899626B (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5340926A (en) * 1983-03-25 1994-08-23 Celltech, Limited Process for the recovery of recombinantly produced protein from insoluble aggregate
US5332805A (en) * 1984-02-03 1994-07-26 Celltech Limited Process for the recovery of recombinantly produced chymosin from insoluble aggregate
US5317324A (en) * 1991-06-20 1994-05-31 Sumitomo Metal Mining Co., Ltd. Printed antenna
US5151501A (en) * 1991-12-20 1992-09-29 American Cyanamid Company Method for solubilization and naturation of somatotropins utilizing sulfolane
AU4665193A (en) * 1992-07-02 1994-01-31 Pitman-Moore, Inc. A process for recovering a recombinant protein, in biologically active form, from a solution containing inactive protein
US5567620A (en) * 1993-01-26 1996-10-22 Eli Lilly And Company Non-denaturing potency assay for somatotropin
ZA94169B (en) * 1993-01-26 1994-09-07 Lilly Co Eli Non-denaturing potency assay for bovine somatotropin
ATE349460T1 (de) * 1998-07-09 2007-01-15 Barofold Inc Rückfaltung von protein-aggregaten und einschlussteilchen durch hohen druck
EP1149160B1 (fr) 1999-01-25 2006-04-19 Novozymes A/S Recuperation d'une proteine a un ph eleve
CA2434045A1 (fr) * 2000-10-31 2002-08-15 The Regents Of The University Of Colorado, A Body Corporate Repliement et desagregation ameliore des proteines sous haute pression
CA2443365C (fr) * 2002-11-19 2010-01-12 F. Hoffmann-La Roche Ag Methodes de production par recombinaison de peptides antifusiogenes
CN101506222B (zh) 2006-07-14 2013-10-02 健泰科生物技术公司 重组蛋白的重折叠
CA2663416A1 (fr) * 2006-09-15 2008-03-20 Barofold, Inc. Traitement de proteines sous haute pression permettant de reduire leur immunogenicite
BR102013026129A2 (pt) * 2013-10-10 2016-05-24 Ouro Fino Participações E Empreendimentos S A processo para a produção de somatotropina bovina recombinate ativa e produto obtido

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2129810B (en) * 1982-06-07 1985-09-18 Celltech Ltd A process for the preparation of chymosin
US4511503A (en) * 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
DE122080T1 (de) * 1983-03-25 1985-05-23 Celltech Ltd., Slough, Berkshire Verfahren zur herstellung eines proteins.
CA1267615A (fr) * 1984-08-27 1990-04-10 Dan Hadary Methode pour la recuperation d'hormones de croissance purifiees a patir de microorganismes modifies par des techniques de genie genetique
US4652630A (en) * 1985-02-22 1987-03-24 Monsanto Company Method of somatotropin naturation
ES8800957A1 (es) * 1985-02-22 1987-12-01 Monsanto Co Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina
US4677196A (en) * 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
AU3439089A (en) * 1988-08-05 1990-03-05 Ralph Akyuz Skin protection lotion
JP2531272B2 (ja) * 1988-08-11 1996-09-04 日本電気株式会社 フレ―ム同期制御方式

Also Published As

Publication number Publication date
KR0148681B1 (ko) 1998-08-01
DK174662B1 (da) 2003-08-18
DK639089D0 (da) 1989-12-15
IE74848B1 (en) 1997-08-13
US5064943A (en) 1991-11-12
FI102179B1 (fi) 1998-10-30
EP0373325B1 (fr) 1996-04-03
CA2005478C (fr) 2000-01-04
AU4675089A (en) 1990-06-21
DE68926153T2 (de) 1996-10-24
DK639089A (da) 1990-06-17
JPH02222697A (ja) 1990-09-05
HU213572B (en) 1997-08-28
IE893561L (en) 1990-06-16
GR3019691T3 (en) 1996-07-31
KR900009971A (ko) 1990-07-06
DE68926153D1 (de) 1996-05-09
EP0373325A3 (fr) 1991-06-12
ZA899626B (en) 1990-09-26
PT92305B (pt) 1995-07-18
FI896021A0 (fi) 1989-12-15
PT92305A (pt) 1990-06-29
NZ231681A (en) 1993-09-27
EP0373325A2 (fr) 1990-06-20
ATE136309T1 (de) 1996-04-15
FI102179B (fi) 1998-10-30
AU628443B2 (en) 1992-09-17
HUT52563A (en) 1990-07-28
IL92135A (en) 1995-05-26
ES2087065T3 (es) 1996-07-16
IL92135A0 (en) 1990-07-12
HU896639D0 (en) 1990-02-28

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Legal Events

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EEER Examination request
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