IL92135A - Method for solubilization and naturation of somatotropin - Google Patents

Method for solubilization and naturation of somatotropin

Info

Publication number: IL92135A
Authority: IL; Israel
Prior art keywords: somatotropin; range; pll; hours; solution
Prior art date: 1988-12-16

Application number

IL9213589A

Other languages

English (en)

Other versions

IL92135A0 (en

Original Assignee

American Cyanamid Co

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1988-12-16

Filing date

1989-10-27

Publication date

1995-05-26

1989-10-27 Application filed by American Cyanamid Co filed Critical American Cyanamid Co

1990-07-12 Publication of IL92135A0 publication Critical patent/IL92135A0/xx

1995-05-26 Publication of IL92135A publication Critical patent/IL92135A/en

Links

108010051696 Growth Hormone Proteins 0.000 title claims abstract description 74
102000018997 Growth Hormone Human genes 0.000 title claims abstract description 74
238000000034 method Methods 0.000 title claims abstract description 42
238000005063 solubilization Methods 0.000 title claims abstract description 15
230000007928 solubilization Effects 0.000 title claims abstract description 15
239000000539 dimer Substances 0.000 claims abstract description 18
239000003795 chemical substances by application Substances 0.000 claims abstract description 11
230000015572 biosynthetic process Effects 0.000 claims abstract description 6
239000000178 monomer Substances 0.000 claims description 22
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
241000283690 Bos taurus Species 0.000 claims description 4
230000003247 decreasing effect Effects 0.000 claims 1
239000003398 denaturant Substances 0.000 abstract description 10
238000004153 renaturation Methods 0.000 abstract description 4
239000012670 alkaline solution Substances 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 description 22
108090000623 proteins and genes Proteins 0.000 description 22
239000000122 growth hormone Substances 0.000 description 11
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
210000004027 cell Anatomy 0.000 description 8
241000588724 Escherichia coli Species 0.000 description 7
230000003196 chaotropic effect Effects 0.000 description 7
239000008188 pellet Substances 0.000 description 6
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 4
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
108010006025 bovine growth hormone Proteins 0.000 description 4
239000004202 carbamide Substances 0.000 description 4
239000012535 impurity Substances 0.000 description 4
239000002243 precursor Substances 0.000 description 4
239000000725 suspension Substances 0.000 description 4
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 3
KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
-1 U.S. Patent No. 4 Chemical compound 0.000 description 3
230000032683 aging Effects 0.000 description 3
125000000539 amino acid group Chemical group 0.000 description 3
238000005227 gel permeation chromatography Methods 0.000 description 3
239000012510 hollow fiber Substances 0.000 description 3
235000019333 sodium laurylsulphate Nutrition 0.000 description 3
BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 2
102000016943 Muramidase Human genes 0.000 description 2
108010014251 Muramidase Proteins 0.000 description 2
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
125000003275 alpha amino acid group Chemical group 0.000 description 2
239000000356 contaminant Substances 0.000 description 2
238000007796 conventional method Methods 0.000 description 2
239000008367 deionised water Substances 0.000 description 2
229910021641 deionized water Inorganic materials 0.000 description 2
230000001419 dependent effect Effects 0.000 description 2
239000003599 detergent Substances 0.000 description 2
230000003292 diminished effect Effects 0.000 description 2
230000000694 effects Effects 0.000 description 2
238000000855 fermentation Methods 0.000 description 2
230000004151 fermentation Effects 0.000 description 2
229960000789 guanidine hydrochloride Drugs 0.000 description 2
PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 2
210000003000 inclusion body Anatomy 0.000 description 2
NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
229960000274 lysozyme Drugs 0.000 description 2
235000010335 lysozyme Nutrition 0.000 description 2
239000004325 lysozyme Substances 0.000 description 2
239000012466 permeate Substances 0.000 description 2
238000003860 storage Methods 0.000 description 2
GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 2
NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
108091006522 Anion exchangers Proteins 0.000 description 1
229920002271 DEAE-Sepharose Polymers 0.000 description 1
108020004414 DNA Proteins 0.000 description 1
108010000521 Human Growth Hormone Proteins 0.000 description 1
102000002265 Human Growth Hormone Human genes 0.000 description 1
239000000854 Human Growth Hormone Substances 0.000 description 1
102000004877 Insulin Human genes 0.000 description 1
108090001061 Insulin Proteins 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
108020004511 Recombinant DNA Proteins 0.000 description 1
230000002411 adverse Effects 0.000 description 1
229910000147 aluminium phosphate Inorganic materials 0.000 description 1
235000011114 ammonium hydroxide Nutrition 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
230000004071 biological effect Effects 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
238000007705 chemical test Methods 0.000 description 1
238000011109 contamination Methods 0.000 description 1
210000000805 cytoplasm Anatomy 0.000 description 1
230000006240 deamidation Effects 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
239000006167 equilibration buffer Substances 0.000 description 1
239000012467 final product Substances 0.000 description 1
239000012634 fragment Substances 0.000 description 1
238000010353 genetic engineering Methods 0.000 description 1
239000008187 granular material Substances 0.000 description 1
239000005556 hormone Substances 0.000 description 1
229940088597 hormone Drugs 0.000 description 1
229940125396 insulin Drugs 0.000 description 1
238000011031 large-scale manufacturing process Methods 0.000 description 1
239000007788 liquid Substances 0.000 description 1
238000012261 overproduction Methods 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
239000013612 plasmid Substances 0.000 description 1
239000002244 precipitate Substances 0.000 description 1
239000000047 product Substances 0.000 description 1
239000011347 resin Substances 0.000 description 1
229920005989 resin Polymers 0.000 description 1
239000002002 slurry Substances 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
230000003381 solubilizing effect Effects 0.000 description 1
239000002904 solvent Substances 0.000 description 1
108700031632 somatrem Proteins 0.000 description 1
241000894007 species Species 0.000 description 1
238000005406 washing Methods 0.000 description 1
239000002699 waste material Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Endocrinology (AREA)
Zoology (AREA)
Genetics & Genomics (AREA)
Medicinal Chemistry (AREA)
Biochemistry (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Gastroenterology & Hepatology (AREA)
Engineering & Computer Science (AREA)
Toxicology (AREA)
Wood Science & Technology (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Microbiology (AREA)
General Engineering & Computer Science (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Peptides Or Proteins (AREA)
Saccharide Compounds (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Medicines Containing Plant Substances (AREA)

IL9213589A 1988-12-16 1989-10-27 Method for solubilization and naturation of somatotropin IL92135A (en)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US07/285,477 US5064943A (en)	1988-12-16	1988-12-16	Method for solubilization and naturation of somatotropin

Publications (2)

Publication Number	Publication Date
IL92135A0 IL92135A0 (en)	1990-07-12
IL92135A true IL92135A (en)	1995-05-26

Family

ID=23094405

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
IL9213589A IL92135A (en)	1988-12-16	1989-10-27	Method for solubilization and naturation of somatotropin

Country Status (18)

Country	Link
US (1)	US5064943A (fr)
EP (1)	EP0373325B1 (fr)
JP (1)	JPH02222697A (fr)
KR (1)	KR0148681B1 (fr)
AT (1)	ATE136309T1 (fr)
AU (1)	AU628443B2 (fr)
CA (1)	CA2005478C (fr)
DE (1)	DE68926153T2 (fr)
DK (1)	DK174662B1 (fr)
ES (1)	ES2087065T3 (fr)
FI (1)	FI102179B (fr)
GR (1)	GR3019691T3 (fr)
HU (1)	HU213572B (fr)
IE (1)	IE74848B1 (fr)
IL (1)	IL92135A (fr)
NZ (1)	NZ231681A (fr)
PT (1)	PT92305B (fr)
ZA (1)	ZA899626B (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5340926A (en) *	1983-03-25	1994-08-23	Celltech, Limited	Process for the recovery of recombinantly produced protein from insoluble aggregate
US5332805A (en) *	1984-02-03	1994-07-26	Celltech Limited	Process for the recovery of recombinantly produced chymosin from insoluble aggregate
US5317324A (en) *	1991-06-20	1994-05-31	Sumitomo Metal Mining Co., Ltd.	Printed antenna
US5151501A (en) *	1991-12-20	1992-09-29	American Cyanamid Company	Method for solubilization and naturation of somatotropins utilizing sulfolane
AU4665193A (en) *	1992-07-02	1994-01-31	Pitman-Moore, Inc.	A process for recovering a recombinant protein, in biologically active form, from a solution containing inactive protein
US5567620A (en) *	1993-01-26	1996-10-22	Eli Lilly And Company	Non-denaturing potency assay for somatotropin
ZA94169B (en) *	1993-01-26	1994-09-07	Lilly Co Eli	Non-denaturing potency assay for bovine somatotropin
ATE349460T1 (de) *	1998-07-09	2007-01-15	Barofold Inc	Rückfaltung von protein-aggregaten und einschlussteilchen durch hohen druck
EP1149160B1 (fr)	1999-01-25	2006-04-19	Novozymes A/S	Recuperation d'une proteine a un ph eleve
CA2434045A1 (fr) *	2000-10-31	2002-08-15	The Regents Of The University Of Colorado, A Body Corporate	Repliement et desagregation ameliore des proteines sous haute pression
CA2443365C (fr) *	2002-11-19	2010-01-12	F. Hoffmann-La Roche Ag	Methodes de production par recombinaison de peptides antifusiogenes
CN101506222B (zh)	2006-07-14	2013-10-02	健泰科生物技术公司	重组蛋白的重折叠
CA2663416A1 (fr) *	2006-09-15	2008-03-20	Barofold, Inc.	Traitement de proteines sous haute pression permettant de reduire leur immunogenicite
BR102013026129A2 (pt) *	2013-10-10	2016-05-24	Ouro Fino Participações E Empreendimentos S A	processo para a produção de somatotropina bovina recombinate ativa e produto obtido

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
GB2129810B (en) *	1982-06-07	1985-09-18	Celltech Ltd	A process for the preparation of chymosin
US4511503A (en) *	1982-12-22	1985-04-16	Genentech, Inc.	Purification and activity assurance of precipitated heterologous proteins
DE122080T1 (de) *	1983-03-25	1985-05-23	Celltech Ltd., Slough, Berkshire	Verfahren zur herstellung eines proteins.
CA1267615A (fr) *	1984-08-27	1990-04-10	Dan Hadary	Methode pour la recuperation d'hormones de croissance purifiees a patir de microorganismes modifies par des techniques de genie genetique
US4652630A (en) *	1985-02-22	1987-03-24	Monsanto Company	Method of somatotropin naturation
ES8800957A1 (es) *	1985-02-22	1987-12-01	Monsanto Co	Un metodo para la solubilizacion y renaturalizacion de proteina somatotropina
US4677196A (en) *	1985-09-06	1987-06-30	International Minerals & Chemical Corp.	Purification and activation of proteins from insoluble inclusion bodies
AU3439089A (en) *	1988-08-05	1990-03-05	Ralph Akyuz	Skin protection lotion
JP2531272B2 (ja) *	1988-08-11	1996-09-04	日本電気株式会社	フレ―ム同期制御方式

1988
- 1988-12-16 US US07/285,477 patent/US5064943A/en not_active Expired - Lifetime
1989
- 1989-10-19 DE DE68926153T patent/DE68926153T2/de not_active Expired - Fee Related
- 1989-10-19 ES ES89119409T patent/ES2087065T3/es not_active Expired - Lifetime
- 1989-10-19 AT AT89119409T patent/ATE136309T1/de not_active IP Right Cessation
- 1989-10-19 EP EP89119409A patent/EP0373325B1/fr not_active Expired - Lifetime
- 1989-10-27 IL IL9213589A patent/IL92135A/en not_active IP Right Cessation
- 1989-11-06 IE IE356189A patent/IE74848B1/en not_active IP Right Cessation
- 1989-11-14 PT PT92305A patent/PT92305B/pt not_active IP Right Cessation
- 1989-12-08 NZ NZ231681A patent/NZ231681A/xx unknown
- 1989-12-14 AU AU46750/89A patent/AU628443B2/en not_active Expired
- 1989-12-14 CA CA002005478A patent/CA2005478C/fr not_active Expired - Fee Related
- 1989-12-14 JP JP1322768A patent/JPH02222697A/ja active Pending
- 1989-12-15 KR KR1019890018683A patent/KR0148681B1/ko not_active Expired - Fee Related
- 1989-12-15 FI FI896021A patent/FI102179B/fi not_active IP Right Cessation
- 1989-12-15 ZA ZA899626A patent/ZA899626B/xx unknown
- 1989-12-15 DK DK198906390A patent/DK174662B1/da not_active IP Right Cessation
- 1989-12-15 HU HU896639A patent/HU213572B/hu not_active IP Right Cessation
1996
- 1996-04-22 GR GR950403213T patent/GR3019691T3/el unknown

Also Published As

Publication number	Publication date
KR0148681B1 (ko)	1998-08-01
DK174662B1 (da)	2003-08-18
DK639089D0 (da)	1989-12-15
IE74848B1 (en)	1997-08-13
US5064943A (en)	1991-11-12
FI102179B1 (fi)	1998-10-30
EP0373325B1 (fr)	1996-04-03
CA2005478C (fr)	2000-01-04
AU4675089A (en)	1990-06-21
DE68926153T2 (de)	1996-10-24
DK639089A (da)	1990-06-17
CA2005478A1 (fr)	1990-06-16
JPH02222697A (ja)	1990-09-05
HU213572B (en)	1997-08-28
IE893561L (en)	1990-06-16
GR3019691T3 (en)	1996-07-31
KR900009971A (ko)	1990-07-06
DE68926153D1 (de)	1996-05-09
EP0373325A3 (fr)	1991-06-12
ZA899626B (en)	1990-09-26
PT92305B (pt)	1995-07-18
FI896021A0 (fi)	1989-12-15
PT92305A (pt)	1990-06-29
NZ231681A (en)	1993-09-27
EP0373325A2 (fr)	1990-06-20
ATE136309T1 (de)	1996-04-15
FI102179B (fi)	1998-10-30
AU628443B2 (en)	1992-09-17
HUT52563A (en)	1990-07-28
ES2087065T3 (es)	1996-07-16
IL92135A0 (en)	1990-07-12
HU896639D0 (en)	1990-02-28

Legal Events

Date	Code	Title
2000-06-01	KB	Patent renewed
2003-12-10	KB	Patent renewed
2008-11-03	MM9K	Patent not in force due to non-payment of renewal fees

Publication	Publication Date	Title
US5064943A (en)	1991-11-12	Method for solubilization and naturation of somatotropin
EP0215625B1 (fr)	1994-11-09	Récupération de protéines
US20160137690A1 (en)	2016-05-19	Refolding of recombinant proteins
AU621111B2 (en)	1992-03-05	Method for naturation of somatotropin protein
DE69636739T2 (de)	2007-10-18	Verfahren zur rückfaltung von menschlichem activin a
EP0312358A2 (fr)	1989-04-19	Méthode pour la promotion de la formation des ponts disulfures dans les protéines recombinantes contenues dans des solutions dénaturantes
EP0547102B1 (fr)	1998-07-08	Solubilisation de proteines sous des formes actives
AU755083B2 (en)	2002-12-05	Method for the production of recombinant peptides with a low amount of trisulfides
EP0432419B1 (fr)	1994-10-19	Méthode de solubilisation et de naturation de la somatotropine utilisant une concentration basse d'urée
EP0291349A2 (fr)	1988-11-17	Procédé de séparation du dodécylsulfate de sodium de solutions de protéines solubilisées par le dodécylsulfate de sodium
AU739394B2 (en)	2001-10-11	Process for the preparation of active somatotropin from inclusion bodies
US6034224A (en)	2000-03-07	Method for solubilization and naturation of somatotropins
US5773588A (en)	1998-06-30	Method for purifying somatotropin monomers
MXPA01001168A (en)	2001-11-21	Process for the preparation of active somatotropin from inclusion bodies