CU23238A1 - CéLULAS HUéSPED PARA LA EXPRESION DE UNA PROTEINA HETEROLOGA - Google Patents

CéLULAS HUéSPED PARA LA EXPRESION DE UNA PROTEINA HETEROLOGA

Info

Publication number
CU23238A1
CU23238A1 CU20020182A CU20020182A CU23238A1 CU 23238 A1 CU23238 A1 CU 23238A1 CU 20020182 A CU20020182 A CU 20020182A CU 20020182 A CU20020182 A CU 20020182A CU 23238 A1 CU23238 A1 CU 23238A1
Authority
CU
Cuba
Prior art keywords
cells
human
recombinant
expression
gene expression
Prior art date
Application number
CU20020182A
Other languages
English (en)
Inventor
Myung-Sam Cho
Original Assignee
Bayer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Corp filed Critical Bayer Corp
Publication of CU23238A1 publication Critical patent/CU23238A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C12N5/163—Animal cells one of the fusion partners being a B or a T lymphocyte
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Las cÉlulas híbridos humana/ humana fueron hechas vía fusion de cÉlulas de rinon embrionico humano (293S) y cÉlulas de linfoma de Burkitt modificadas (2B8). Las cÉlulas fusion son Utiles como cÉlulas huÉspedes para la expresion recombinante de gene de mamíferos. Las ventajas del uso de estos clones híbridos de rinon humano- y cÉlulas B, llamadas HKBs, para expresion de gen de mamífero, incluyen (i) las cÉlulas son negativas para la expresion de inmunoglobulina, (ii) las cÉlulas crecen facilmemte en plasma de medio libre de proteína (con o sin la adicion de insulina recombinante) como cultura de suspension en un frasco de batir o en un fermentador (iii) las cÉlulas son muy susceptibles para la transfeccion de ADN y (iv) las cÉlulas segregan altos niveles de proteínas recombinantes heterologas, tales como anticuerpos monoclonales recombinantes, ICAM-1 soluble, rIL-4 y rFVIII.
CU20020182A 1998-12-10 2002-08-26 CéLULAS HUéSPED PARA LA EXPRESION DE UNA PROTEINA HETEROLOGA CU23238A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/209,920 US6136599A (en) 1998-12-10 1998-12-10 Human hybrid host cell for mammalian gene expression

Publications (1)

Publication Number Publication Date
CU23238A1 true CU23238A1 (es) 2007-09-26

Family

ID=22780882

Family Applications (2)

Application Number Title Priority Date Filing Date
CU20010130A CU23382A3 (es) 1998-12-10 2001-06-07 Metodo de producir celulas utiles para la expresion de una proteina heterologa
CU20020182A CU23238A1 (es) 1998-12-10 2002-08-26 CéLULAS HUéSPED PARA LA EXPRESION DE UNA PROTEINA HETEROLOGA

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CU20010130A CU23382A3 (es) 1998-12-10 2001-06-07 Metodo de producir celulas utiles para la expresion de una proteina heterologa

Country Status (27)

Country Link
US (1) US6136599A (es)
EP (1) EP1137768B1 (es)
JP (3) JP4332300B2 (es)
KR (1) KR100627753B1 (es)
CN (1) CN1230532C (es)
AT (1) ATE306547T1 (es)
AU (1) AU757188B2 (es)
BG (1) BG65263B1 (es)
BR (1) BRPI9916095B8 (es)
CA (1) CA2353698C (es)
CU (2) CU23382A3 (es)
CZ (1) CZ302677B6 (es)
DE (1) DE69927707T2 (es)
DK (1) DK1137768T3 (es)
ES (1) ES2249929T3 (es)
HU (1) HU229775B1 (es)
ID (1) ID30448A (es)
IL (2) IL143351A0 (es)
NO (1) NO330548B1 (es)
PL (1) PL205549B1 (es)
RO (1) RO121563B1 (es)
RU (1) RU2228355C2 (es)
SI (1) SI20693B (es)
SK (1) SK287888B6 (es)
TR (1) TR200101642T2 (es)
UA (1) UA77642C2 (es)
WO (1) WO2000034462A1 (es)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6358703B1 (en) 1998-12-10 2002-03-19 Bayer Corporation Expression system for factor VIII
US6136599A (en) * 1998-12-10 2000-10-24 Bayer Corporation Human hybrid host cell for mammalian gene expression
US6348192B1 (en) * 1999-05-11 2002-02-19 Bayer Corporation Interleukin-2 mutein expressed from mammalian cells
US20040235715A1 (en) * 1999-11-12 2004-11-25 Bayer Corporation Method of producing glycosylated bikunin
US6569657B1 (en) * 2000-06-27 2003-05-27 Millennium Pharmaceuticals, Inc. 32140, a novel human aldehyde dehydrogenase and uses therefor
US7094587B2 (en) * 2000-06-27 2006-08-22 Millennium Pharmaceuticals, Inc. 16002 Molecules and uses therefor
MY139948A (en) * 2000-09-28 2009-11-30 Bayer Corp Enhanced transfection system
US7723102B2 (en) * 2000-09-28 2010-05-25 Bayer Corporation Enhanced transfection system
US20060073141A1 (en) * 2001-06-28 2006-04-06 Domantis Limited Compositions and methods for treating inflammatory disorders
KR100496861B1 (ko) 2002-09-26 2005-06-22 삼성전자주식회사 하나의 핸들러에 2개 이상의 테스트 보드를 갖는 테스트장비 및 그 테스트 방법
US7285269B2 (en) 2002-12-02 2007-10-23 Amgen Fremont, Inc. Antibodies directed to tumor necrosis factor
US7244616B2 (en) 2003-06-27 2007-07-17 Bayer Pharmaceuticals Corporation Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells
PT2363414T (pt) 2004-11-12 2022-08-04 Bayer Healthcare Llc Modificação de fviii direcionada a sítio
JP2010528647A (ja) 2007-06-06 2010-08-26 ドマンティス リミテッド ポリペプチド、抗体可変ドメインおよびアンタゴニスト
JP5628790B2 (ja) 2008-04-07 2014-11-19 バイエル・ヘルスケア・エルエルシー 糖タンパク質の組換え生産の方法
KR101005967B1 (ko) * 2008-04-12 2011-01-05 (주)셀트리온 우수한 재조합 단백질을 생산하기 위한 인간 숙주 세포
US9090948B2 (en) 2008-09-30 2015-07-28 Abbott Molecular Inc. Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples
CN102612556A (zh) * 2009-06-10 2012-07-25 Bts研究国际股份有限公司 制备杂交细胞/嵌合细胞的方法及其应用
US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein
WO2018017144A1 (en) 2016-07-19 2018-01-25 Brandeis University Compositions and methods for identifying rna binding polypeptide targets
AU2019270342A1 (en) 2018-05-16 2020-10-29 Morphosys Ag Antibodies targeting glycoprotein VI
CA3125527A1 (en) 2018-12-20 2020-06-25 Orionis Biosciences, Inc. Particle based small molecule-protein complex trap
JP7617012B2 (ja) 2019-03-14 2025-01-17 モルフォシス・アーゲー Ｃ５ａＲを標的とする抗体
EP3980524A4 (en) * 2019-06-10 2023-01-11 Cho Plus Inc. Cell lines for high level production of protein-based pharmaceuticals
US20230357398A1 (en) 2020-09-24 2023-11-09 Morphosys Ag Novel human antibodies binding to human cd3 epsilon
CN113604425B (zh) * 2021-06-22 2022-10-18 中山康天晟合生物技术有限公司 一种适应无血清培养基环境的wayne293 lvpro细胞及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6045849B2 (ja) * 1980-08-25 1985-10-12 林原 健 ヒトエリトロポエチンの製造方法
JPS6030654B2 (ja) * 1980-12-31 1985-07-17 株式会社林原生物化学研究所 ヒトコロニ−刺激因子の製造方法
US6136599A (en) * 1998-12-10 2000-10-24 Bayer Corporation Human hybrid host cell for mammalian gene expression

Also Published As

Publication number Publication date
SK7912001A3 (en) 2002-03-05
DE69927707D1 (de) 2006-02-23
NO330548B1 (no) 2011-05-16
IL143351A (en) 2009-12-24
EP1137768A1 (en) 2001-10-04
PL205549B1 (pl) 2010-04-30
AU1938300A (en) 2000-06-26
EP1137768A4 (en) 2002-09-18
JP2014131513A (ja) 2014-07-17
HUP0104681A2 (hu) 2002-04-29
KR20020002360A (ko) 2002-01-09
TR200101642T2 (tr) 2001-11-21
UA77642C2 (en) 2007-01-15
IL143351A0 (en) 2002-04-21
CA2353698C (en) 2012-02-21
CN1230532C (zh) 2005-12-07
BG65263B1 (bg) 2007-10-31
SI20693B (sl) 2008-12-31
JP2002531125A (ja) 2002-09-24
ES2249929T3 (es) 2006-04-01
CZ20012023A3 (cs) 2001-10-17
KR100627753B1 (ko) 2006-09-26
SI20693A (sl) 2002-04-30
HUP0104681A3 (en) 2005-10-28
CN1339063A (zh) 2002-03-06
JP5826311B2 (ja) 2015-12-02
ATE306547T1 (de) 2005-10-15
JP2009148295A (ja) 2009-07-09
NO20012806D0 (no) 2001-06-07
DE69927707T2 (de) 2006-07-13
ID30448A (id) 2001-12-06
BRPI9916095B8 (pt) 2021-05-25
BR9916095A (pt) 2001-09-04
SK287888B6 (sk) 2012-03-02
CZ302677B6 (cs) 2011-08-31
RO121563B1 (ro) 2007-11-30
JP5508740B2 (ja) 2014-06-04
BG105566A (en) 2002-03-29
BRPI9916095B1 (pt) 2015-10-13
HU229775B1 (en) 2014-07-28
NO20012806L (no) 2001-06-07
CA2353698A1 (en) 2000-06-15
RU2228355C2 (ru) 2004-05-10
CU23382A3 (es) 2009-06-25
US6136599A (en) 2000-10-24
EP1137768B1 (en) 2005-10-12
WO2000034462A1 (en) 2000-06-15
AU757188B2 (en) 2003-02-06
DK1137768T3 (da) 2005-12-19
JP4332300B2 (ja) 2009-09-16

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