DD284048A5 - Verfahren zur herstellung einer pflanzenzelle bzw. eines pflanzenteils oder einer pflanze mit einer derartigen pflanzenzelle - Google Patents

Verfahren zur herstellung einer pflanzenzelle bzw. eines pflanzenteils oder einer pflanze mit einer derartigen pflanzenzelle Download PDF

Info

Publication number: DD284048A5
Authority: DD; German Democratic Republic
Prior art keywords: giy; nitrilase; ala; giu; fragment
Prior art date: 1987-07-08

Application number

DD88330727A

Other languages

German (de)

English (en)

Inventor

David M Stalker

Original Assignee

��`��@��k��

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1987-07-08

Filing date

1988-07-08

Publication date

1990-10-31

1988-07-08 Application filed by ��`��@��k�� filed Critical ��`��@��k��

1990-10-31 Publication of DD284048A5 publication Critical patent/DD284048A5/de

Links

238000004519 manufacturing process Methods 0.000 title abstract description 5
108010033272 Nitrilase Proteins 0.000 claims abstract description 96
108090000623 proteins and genes Proteins 0.000 claims abstract description 82
UPMXNNIRAGDFEH-UHFFFAOYSA-N 3,5-dibromo-4-hydroxybenzonitrile Chemical compound OC1=C(Br)C=C(C#N)C=C1Br UPMXNNIRAGDFEH-UHFFFAOYSA-N 0.000 claims abstract description 74
239000005489 Bromoxynil Substances 0.000 claims abstract description 72
230000000694 effects Effects 0.000 claims abstract description 25
150000001413 amino acids Chemical class 0.000 claims abstract description 16
102000004169 proteins and genes Human genes 0.000 claims abstract description 16
241000894006 Bacteria Species 0.000 claims abstract description 11
238000003776 cleavage reaction Methods 0.000 claims abstract description 10
230000007017 scission Effects 0.000 claims abstract description 10
230000002103 transcriptional effect Effects 0.000 claims abstract description 8
238000006467 substitution reaction Methods 0.000 claims abstract description 7
239000000758 substrate Substances 0.000 claims abstract description 7
CVNOWLNNPYYEOH-UHFFFAOYSA-N 4-cyanophenol Chemical class OC1=CC=C(C#N)C=C1 CVNOWLNNPYYEOH-UHFFFAOYSA-N 0.000 claims abstract description 6
238000000034 method Methods 0.000 claims description 14
230000001105 regulatory effect Effects 0.000 claims description 11
238000013518 transcription Methods 0.000 claims description 10
230000035897 transcription Effects 0.000 claims description 10
230000008569 process Effects 0.000 claims description 3
230000002363 herbicidal effect Effects 0.000 abstract description 4
150000002825 nitriles Chemical class 0.000 abstract description 3
239000012634 fragment Substances 0.000 description 114
239000013612 plasmid Substances 0.000 description 90
241000196324 Embryophyta Species 0.000 description 54
210000004027 cell Anatomy 0.000 description 43
108020004414 DNA Proteins 0.000 description 37
241000588724 Escherichia coli Species 0.000 description 32
BYXHQQCXAJARLQ-ZLUOBGJFSA-N Ala-Ala-Ala Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(O)=O BYXHQQCXAJARLQ-ZLUOBGJFSA-N 0.000 description 27
102000004190 Enzymes Human genes 0.000 description 24
108090000790 Enzymes Proteins 0.000 description 24
JFDZBHWFFUWGJE-UHFFFAOYSA-N benzonitrile Chemical compound N#CC1=CC=CC=C1 JFDZBHWFFUWGJE-UHFFFAOYSA-N 0.000 description 24
229940088598 enzyme Drugs 0.000 description 24
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 22
244000061176 Nicotiana tabacum Species 0.000 description 17
235000002637 Nicotiana tabacum Nutrition 0.000 description 17
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 16
241000588744 Klebsiella pneumoniae subsp. ozaenae Species 0.000 description 16
239000000872 buffer Substances 0.000 description 16
SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 16
238000002360 preparation method Methods 0.000 description 16
229930027917 kanamycin Natural products 0.000 description 14
229960000318 kanamycin Drugs 0.000 description 14
229930182823 kanamycin A Natural products 0.000 description 14
239000002609 medium Substances 0.000 description 14
108020004705 Codon Proteins 0.000 description 11
239000004009 herbicide Substances 0.000 description 11
239000011780 sodium chloride Substances 0.000 description 11
239000013598 vector Substances 0.000 description 11
108091026890 Coding region Proteins 0.000 description 9
108091028043 Nucleic acid sequence Proteins 0.000 description 9
238000010367 cloning Methods 0.000 description 9
238000010276 construction Methods 0.000 description 9
239000000287 crude extract Substances 0.000 description 9
229910052757 nitrogen Inorganic materials 0.000 description 9
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 8
108010083942 mannopine synthase Proteins 0.000 description 8
108700039691 Genetic Promoter Regions Proteins 0.000 description 7
238000000746 purification Methods 0.000 description 7
108091008146 restriction endonucleases Proteins 0.000 description 7
239000002689 soil Substances 0.000 description 7
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
240000003768 Solanum lycopersicum Species 0.000 description 6
229960005091 chloramphenicol Drugs 0.000 description 6
WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 6
238000002474 experimental method Methods 0.000 description 6
SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 6
230000010354 integration Effects 0.000 description 6
108010025153 lysyl-alanyl-alanine Proteins 0.000 description 6
241000894007 species Species 0.000 description 6
210000001519 tissue Anatomy 0.000 description 6
BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 5
241000589155 Agrobacterium tumefaciens Species 0.000 description 5
108010066133 D-octopine dehydrogenase Proteins 0.000 description 5
102000004594 DNA Polymerase I Human genes 0.000 description 5
108010017826 DNA Polymerase I Proteins 0.000 description 5
239000007983 Tris buffer Substances 0.000 description 5
108010062796 arginyllysine Proteins 0.000 description 5
230000001580 bacterial effect Effects 0.000 description 5
230000015556 catabolic process Effects 0.000 description 5
150000001875 compounds Chemical class 0.000 description 5
238000006731 degradation reaction Methods 0.000 description 5
230000029087 digestion Effects 0.000 description 5
VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 5
230000001939 inductive effect Effects 0.000 description 5
NRXQIUSYPAHGNM-UHFFFAOYSA-N ioxynil Chemical compound OC1=C(I)C=C(C#N)C=C1I NRXQIUSYPAHGNM-UHFFFAOYSA-N 0.000 description 5
108020004999 messenger RNA Proteins 0.000 description 5
230000036961 partial effect Effects 0.000 description 5
239000000047 product Substances 0.000 description 5
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 5
241000589158 Agrobacterium Species 0.000 description 4
NPDLYUOYAGBHFB-WDSKDSINSA-N Asn-Arg Chemical compound NC(=O)C[C@H](N)C(=O)N[C@H](C(O)=O)CCCN=C(N)N NPDLYUOYAGBHFB-WDSKDSINSA-N 0.000 description 4
HXWUJJADFMXNKA-BQBZGAKWSA-N Asn-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](N)CC(N)=O HXWUJJADFMXNKA-BQBZGAKWSA-N 0.000 description 4
UMHUHHJMEXNSIV-CIUDSAMLSA-N Asp-Leu-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC(O)=O UMHUHHJMEXNSIV-CIUDSAMLSA-N 0.000 description 4
DWBZEJHQQIURML-IMJSIDKUSA-N Asp-Ser Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CO)C(O)=O DWBZEJHQQIURML-IMJSIDKUSA-N 0.000 description 4
XXAMCEGRCZQGEM-ZLUOBGJFSA-N Asp-Ser-Asn Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(O)=O XXAMCEGRCZQGEM-ZLUOBGJFSA-N 0.000 description 4
102000053602 DNA Human genes 0.000 description 4
108010054576 Deoxyribonuclease EcoRI Proteins 0.000 description 4
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
VCHVSKNMTXWIIP-SRVKXCTJSA-N Leu-Lys-Ser Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O VCHVSKNMTXWIIP-SRVKXCTJSA-N 0.000 description 4
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 4
ROHDXJUFQVRDAV-UWVGGRQHSA-N Phe-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CC=CC=C1 ROHDXJUFQVRDAV-UWVGGRQHSA-N 0.000 description 4
GLEOIKLQBZNKJZ-WDSKDSINSA-N Pro-Asp Chemical compound OC(=O)C[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1 GLEOIKLQBZNKJZ-WDSKDSINSA-N 0.000 description 4
GVUVRRPYYDHHGK-VQVTYTSYSA-N Pro-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1 GVUVRRPYYDHHGK-VQVTYTSYSA-N 0.000 description 4
KCNSGAMPBPYUAI-CIUDSAMLSA-N Ser-Leu-Asn Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O KCNSGAMPBPYUAI-CIUDSAMLSA-N 0.000 description 4
WBAXJMCUFIXCNI-WDSKDSINSA-N Ser-Pro Chemical compound OC[C@H](N)C(=O)N1CCC[C@H]1C(O)=O WBAXJMCUFIXCNI-WDSKDSINSA-N 0.000 description 4
239000004098 Tetracycline Substances 0.000 description 4
IQHUITKNHOKGFC-MIMYLULJSA-N Thr-Phe Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 IQHUITKNHOKGFC-MIMYLULJSA-N 0.000 description 4
GXDLGHLJTHMDII-WISUUJSJSA-N Thr-Ser Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](CO)C(O)=O GXDLGHLJTHMDII-WISUUJSJSA-N 0.000 description 4
241000700605 Viruses Species 0.000 description 4
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 4
229910052921 ammonium sulfate Inorganic materials 0.000 description 4
235000011130 ammonium sulphate Nutrition 0.000 description 4
238000009472 formulation Methods 0.000 description 4
239000000499 gel Substances 0.000 description 4
WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 4
108010038320 lysylphenylalanine Proteins 0.000 description 4
244000005700 microbiome Species 0.000 description 4
239000000203 mixture Substances 0.000 description 4
108010051242 phenylalanylserine Proteins 0.000 description 4
229920001184 polypeptide Polymers 0.000 description 4
108090000765 processed proteins & peptides Proteins 0.000 description 4
102000004196 processed proteins & peptides Human genes 0.000 description 4
239000000523 sample Substances 0.000 description 4
108010026333 seryl-proline Proteins 0.000 description 4
108010071207 serylmethionine Proteins 0.000 description 4
239000006228 supernatant Substances 0.000 description 4
229960002180 tetracycline Drugs 0.000 description 4
229930101283 tetracycline Natural products 0.000 description 4
235000019364 tetracycline Nutrition 0.000 description 4
150000003522 tetracyclines Chemical class 0.000 description 4
230000009466 transformation Effects 0.000 description 4
238000011144 upstream manufacturing Methods 0.000 description 4
UWQJHXKARZWDIJ-ZLUOBGJFSA-N Ala-Ala-Cys Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CS)C(O)=O UWQJHXKARZWDIJ-ZLUOBGJFSA-N 0.000 description 3
QUIGLPSHIFPEOV-CIUDSAMLSA-N Ala-Lys-Ala Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(O)=O QUIGLPSHIFPEOV-CIUDSAMLSA-N 0.000 description 3
OSRZOHXQCUFIQG-FPMFFAJLSA-N Ala-Phe-Pro Chemical compound C([C@H](NC(=O)[C@@H]([NH3+])C)C(=O)N1[C@H](CCC1)C([O-])=O)C1=CC=CC=C1 OSRZOHXQCUFIQG-FPMFFAJLSA-N 0.000 description 3
XPSGESXVBSQZPL-SRVKXCTJSA-N Arg-Arg-Arg Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O XPSGESXVBSQZPL-SRVKXCTJSA-N 0.000 description 3
DQNLFLGFZAUIOW-FXQIFTODSA-N Arg-Cys-Ala Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](C)C(O)=O DQNLFLGFZAUIOW-FXQIFTODSA-N 0.000 description 3
RYQSYXFGFOTJDJ-RHYQMDGZSA-N Arg-Thr-Leu Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(C)C)C(O)=O RYQSYXFGFOTJDJ-RHYQMDGZSA-N 0.000 description 3
JTXVXGXTRXMOFJ-FXQIFTODSA-N Asn-Pro-Asn Chemical compound NC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(N)=O)C(O)=O JTXVXGXTRXMOFJ-FXQIFTODSA-N 0.000 description 3
WSWYMRLTJVKRCE-ZLUOBGJFSA-N Asp-Ala-Asp Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(O)=O WSWYMRLTJVKRCE-ZLUOBGJFSA-N 0.000 description 3
ILJQISGMGXRZQQ-IHRRRGAJSA-N Asp-Arg-Tyr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O ILJQISGMGXRZQQ-IHRRRGAJSA-N 0.000 description 3
ZKAOJVJQGVUIIU-GUBZILKMSA-N Asp-Pro-Arg Chemical compound OC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(N)=N)C(O)=O ZKAOJVJQGVUIIU-GUBZILKMSA-N 0.000 description 3
WSDOHRLQDGAOGU-BQBZGAKWSA-N His-Asn Chemical compound NC(=O)C[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CN=CN1 WSDOHRLQDGAOGU-BQBZGAKWSA-N 0.000 description 3
101000595669 Homo sapiens Pituitary homeobox 2 Proteins 0.000 description 3
BAJIJEGGUYXZGC-CIUDSAMLSA-N Leu-Asn-Cys Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(=O)N)C(=O)N[C@@H](CS)C(=O)O)N BAJIJEGGUYXZGC-CIUDSAMLSA-N 0.000 description 3
SBANPBVRHYIMRR-UHFFFAOYSA-N Leu-Ser-Pro Natural products CC(C)CC(N)C(=O)NC(CO)C(=O)N1CCCC1C(O)=O SBANPBVRHYIMRR-UHFFFAOYSA-N 0.000 description 3
BQVUABVGYYSDCJ-ZFWWWQNUSA-N Leu-Trp Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](N)CC(C)C)C(O)=O)=CNC2=C1 BQVUABVGYYSDCJ-ZFWWWQNUSA-N 0.000 description 3
235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
IOQWIOPSKJOEKI-SRVKXCTJSA-N Lys-Ser-Leu Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(O)=O IOQWIOPSKJOEKI-SRVKXCTJSA-N 0.000 description 3
BQVUABVGYYSDCJ-UHFFFAOYSA-N Nalpha-L-Leucyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)C(N)CC(C)C)C(O)=O)=CNC2=C1 BQVUABVGYYSDCJ-UHFFFAOYSA-N 0.000 description 3
206010028980 Neoplasm Diseases 0.000 description 3
108091092724 Noncoding DNA Proteins 0.000 description 3
229930182555 Penicillin Natural products 0.000 description 3
102100036090 Pituitary homeobox 2 Human genes 0.000 description 3
QVIZLAUEAMQKGS-GUBZILKMSA-N Pro-Asp-Met Chemical compound CSCC[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@@H]1CCCN1 QVIZLAUEAMQKGS-GUBZILKMSA-N 0.000 description 3
229920005654 Sephadex Polymers 0.000 description 3
239000012507 Sephadex™ Substances 0.000 description 3
XJDMUQCLVSCRSJ-VZFHVOOUSA-N Ser-Thr-Ala Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(O)=O XJDMUQCLVSCRSJ-VZFHVOOUSA-N 0.000 description 3
108091081024 Start codon Proteins 0.000 description 3
JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 3
BQBCIBCLXBKYHW-CSMHCCOUSA-N Thr-Leu Chemical compound CC(C)C[C@@H](C([O-])=O)NC(=O)[C@@H]([NH3+])[C@@H](C)O BQBCIBCLXBKYHW-CSMHCCOUSA-N 0.000 description 3
NCXVJIQMWSGRHY-KXNHARMFSA-N Thr-Leu-Pro Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@@H]1C(=O)O)N)O NCXVJIQMWSGRHY-KXNHARMFSA-N 0.000 description 3
YKRQRPFODDJQTC-CSMHCCOUSA-N Thr-Lys Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@H](C(O)=O)CCCCN YKRQRPFODDJQTC-CSMHCCOUSA-N 0.000 description 3
YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
NESIQDDPEFTWAH-BPUTZDHNSA-N Trp-Met-Asp Chemical compound [H]N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(O)=O)C(O)=O NESIQDDPEFTWAH-BPUTZDHNSA-N 0.000 description 3
VCXWRWYFJLXITF-AUTRQRHGSA-N Tyr-Ala-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 VCXWRWYFJLXITF-AUTRQRHGSA-N 0.000 description 3
241001672648 Vieira Species 0.000 description 3
239000002253 acid Substances 0.000 description 3
150000007513 acids Chemical class 0.000 description 3
229910021529 ammonia Inorganic materials 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
210000000941 bile Anatomy 0.000 description 3
210000000349 chromosome Anatomy 0.000 description 3
238000005520 cutting process Methods 0.000 description 3
238000001962 electrophoresis Methods 0.000 description 3
230000002068 genetic effect Effects 0.000 description 3
230000012010 growth Effects 0.000 description 3
238000010348 incorporation Methods 0.000 description 3
239000003617 indole-3-acetic acid Substances 0.000 description 3
108010090333 leucyl-lysyl-proline Proteins 0.000 description 3
239000007788 liquid Substances 0.000 description 3
239000002207 metabolite Substances 0.000 description 3
239000006870 ms-medium Substances 0.000 description 3
MWUXSHHQAYIFBG-UHFFFAOYSA-N nitrogen oxide Inorganic materials O=[N] MWUXSHHQAYIFBG-UHFFFAOYSA-N 0.000 description 3
239000002773 nucleotide Substances 0.000 description 3
125000003729 nucleotide group Chemical group 0.000 description 3
229940049954 penicillin Drugs 0.000 description 3
150000003839 salts Chemical class 0.000 description 3
239000000243 solution Substances 0.000 description 3
239000000126 substance Substances 0.000 description 3
KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 3
229960003495 thiamine Drugs 0.000 description 3
235000019157 thiamine Nutrition 0.000 description 3
239000011721 thiamine Substances 0.000 description 3
230000005026 transcription initiation Effects 0.000 description 3
230000009261 transgenic effect Effects 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
LWTDZKXXJRRKDG-KXBFYZLASA-N (-)-phaseollin Chemical compound C1OC2=CC(O)=CC=C2[C@H]2[C@@H]1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-KXBFYZLASA-N 0.000 description 2
108020005029 5' Flanking Region Proteins 0.000 description 2
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 2
229920001817 Agar Polymers 0.000 description 2
241000589156 Agrobacterium rhizogenes Species 0.000 description 2
FJVAQLJNTSUQPY-CIUDSAMLSA-N Ala-Ala-Lys Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CCCCN FJVAQLJNTSUQPY-CIUDSAMLSA-N 0.000 description 2
HJVGMOYJDDXLMI-AVGNSLFASA-N Arg-Arg-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@@H](N)CCCNC(N)=N HJVGMOYJDDXLMI-AVGNSLFASA-N 0.000 description 2
OVQJAKFLFTZDNC-GUBZILKMSA-N Arg-Pro-Asp Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(O)=O OVQJAKFLFTZDNC-GUBZILKMSA-N 0.000 description 2
BFDDUDQCPJWQRQ-IHRRRGAJSA-N Arg-Tyr-Cys Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)N[C@@H](CS)C(=O)O)NC(=O)[C@H](CCCN=C(N)N)N)O BFDDUDQCPJWQRQ-IHRRRGAJSA-N 0.000 description 2
UKGGPJNBONZZCM-WDSKDSINSA-N Asp-Pro Chemical compound OC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(O)=O UKGGPJNBONZZCM-WDSKDSINSA-N 0.000 description 2
RVMXMLSYBTXCAV-VEVYYDQMSA-N Asp-Pro-Thr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(O)=O RVMXMLSYBTXCAV-VEVYYDQMSA-N 0.000 description 2
108091003079 Bovine Serum Albumin Proteins 0.000 description 2
101000583086 Bunodosoma granuliferum Delta-actitoxin-Bgr2b Proteins 0.000 description 2
229920000742 Cotton Polymers 0.000 description 2
102100031780 Endonuclease Human genes 0.000 description 2
108010042407 Endonucleases Proteins 0.000 description 2
108060002716 Exonuclease Proteins 0.000 description 2
230000005526 G1 to G0 transition Effects 0.000 description 2
108700007698 Genetic Terminator Regions Proteins 0.000 description 2
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
244000068988 Glycine max Species 0.000 description 2
235000010469 Glycine max Nutrition 0.000 description 2
241000208818 Helianthus Species 0.000 description 2
235000003222 Helianthus annuus Nutrition 0.000 description 2
KRBMQYPTDYSENE-BQBZGAKWSA-N His-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CNC=N1 KRBMQYPTDYSENE-BQBZGAKWSA-N 0.000 description 2
JATYGDHMDRAISQ-KKUMJFAQSA-N His-Tyr-Ser Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CO)C(O)=O JATYGDHMDRAISQ-KKUMJFAQSA-N 0.000 description 2
VHJLVAABSRFDPM-IMJSIDKUSA-N L-1,4-dithiothreitol Chemical compound SC[C@H](O)[C@@H](O)CS VHJLVAABSRFDPM-IMJSIDKUSA-N 0.000 description 2
MMEDVBWCMGRKKC-GARJFASQSA-N Leu-Asp-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)N1CCC[C@@H]1C(=O)O)N MMEDVBWCMGRKKC-GARJFASQSA-N 0.000 description 2
HDHQQEDVWQGBEE-DCAQKATOSA-N Leu-Met-Ser Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CO)C(O)=O HDHQQEDVWQGBEE-DCAQKATOSA-N 0.000 description 2
VTJUNIYRYIAIHF-IUCAKERBSA-N Leu-Pro Chemical compound CC(C)C[C@H](N)C(=O)N1CCC[C@H]1C(O)=O VTJUNIYRYIAIHF-IUCAKERBSA-N 0.000 description 2
IWMJFLJQHIDZQW-KKUMJFAQSA-N Leu-Ser-Phe Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CO)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 IWMJFLJQHIDZQW-KKUMJFAQSA-N 0.000 description 2
RBEATVHTWHTHTJ-KKUMJFAQSA-N Lys-Leu-Lys Chemical compound NCCCC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(O)=O RBEATVHTWHTHTJ-KKUMJFAQSA-N 0.000 description 2
ZVZRQKJOQQAFCF-ULQDDVLXSA-N Lys-Tyr-Arg Chemical compound [H]N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O ZVZRQKJOQQAFCF-ULQDDVLXSA-N 0.000 description 2
SQRLLZAQNOQCEG-KKUMJFAQSA-N Lys-Tyr-Ser Chemical compound NCCCC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CO)C(O)=O)CC1=CC=C(O)C=C1 SQRLLZAQNOQCEG-KKUMJFAQSA-N 0.000 description 2
MCNGIXXCMJAURZ-VEVYYDQMSA-N Met-Asp-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCSC)N)O MCNGIXXCMJAURZ-VEVYYDQMSA-N 0.000 description 2
XPVCDCMPKCERFT-GUBZILKMSA-N Met-Ser-Arg Chemical compound [H]N[C@@H](CCSC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O XPVCDCMPKCERFT-GUBZILKMSA-N 0.000 description 2
241000208134 Nicotiana rustica Species 0.000 description 2
239000000020 Nitrocellulose Substances 0.000 description 2
108700026244 Open Reading Frames Proteins 0.000 description 2
229920001213 Polysorbate 20 Polymers 0.000 description 2
SPLBRAKYXGOFSO-UNQGMJICSA-N Pro-Phe-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CC1=CC=CC=C1)NC(=O)[C@@H]2CCCN2)O SPLBRAKYXGOFSO-UNQGMJICSA-N 0.000 description 2
PKHDJFHFMGQMPS-RCWTZXSCSA-N Pro-Thr-Arg Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O PKHDJFHFMGQMPS-RCWTZXSCSA-N 0.000 description 2
IDCKUIWEIZYVSO-WFBYXXMGSA-N Ser-Ala-Trp Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](NC(=O)[C@@H](N)CO)C)C(O)=O)=CNC2=C1 IDCKUIWEIZYVSO-WFBYXXMGSA-N 0.000 description 2
FLONGDPORFIVQW-XGEHTFHBSA-N Ser-Pro-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CO FLONGDPORFIVQW-XGEHTFHBSA-N 0.000 description 2
DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 2
JMQUAZXYFAEOIH-XGEHTFHBSA-N Thr-Arg-Cys Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CS)C(=O)O)N)O JMQUAZXYFAEOIH-XGEHTFHBSA-N 0.000 description 2
PKXHGEXFMIZSER-QTKMDUPCSA-N Thr-Arg-His Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N)O PKXHGEXFMIZSER-QTKMDUPCSA-N 0.000 description 2
VGYVVSQFSSKZRJ-OEAJRASXSA-N Thr-Phe-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)[C@H](O)C)CC1=CC=CC=C1 VGYVVSQFSSKZRJ-OEAJRASXSA-N 0.000 description 2
GFUOTIPYXKAPAH-BVSLBCMMSA-N Trp-Pro-Phe Chemical compound [H]N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O GFUOTIPYXKAPAH-BVSLBCMMSA-N 0.000 description 2
OGPKMBOPMDTEDM-IHRRRGAJSA-N Tyr-Met-Ser Chemical compound CSCC[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CC1=CC=C(C=C1)O)N OGPKMBOPMDTEDM-IHRRRGAJSA-N 0.000 description 2
VNYDHJARLHNEGA-RYUDHWBXSA-N Tyr-Pro Chemical compound C([C@H](N)C(=O)N1[C@@H](CCC1)C(O)=O)C1=CC=C(O)C=C1 VNYDHJARLHNEGA-RYUDHWBXSA-N 0.000 description 2
ZSXJENBJGRHKIG-UWVGGRQHSA-N Tyr-Ser Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 ZSXJENBJGRHKIG-UWVGGRQHSA-N 0.000 description 2
RWOKVQUCENPXGE-IHRRRGAJSA-N Tyr-Ser-Arg Chemical compound [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O RWOKVQUCENPXGE-IHRRRGAJSA-N 0.000 description 2
MQGGXGKQSVEQHR-KKUMJFAQSA-N Tyr-Ser-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC1=CC=C(O)C=C1 MQGGXGKQSVEQHR-KKUMJFAQSA-N 0.000 description 2
239000002671 adjuvant Substances 0.000 description 2
239000008272 agar Substances 0.000 description 2
239000011543 agarose gel Substances 0.000 description 2
108010068380 arginylarginine Proteins 0.000 description 2
108010060035 arginylproline Proteins 0.000 description 2
108010093581 aspartyl-proline Proteins 0.000 description 2
150000008359 benzonitriles Chemical class 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
229940098773 bovine serum albumin Drugs 0.000 description 2
229910052799 carbon Inorganic materials 0.000 description 2
238000006243 chemical reaction Methods 0.000 description 2
239000002299 complementary DNA Substances 0.000 description 2
230000021615 conjugation Effects 0.000 description 2
239000013601 cosmid vector Substances 0.000 description 2
231100000433 cytotoxic Toxicity 0.000 description 2
230000001472 cytotoxic effect Effects 0.000 description 2
238000012217 deletion Methods 0.000 description 2
230000037430 deletion Effects 0.000 description 2
238000001784 detoxification Methods 0.000 description 2
238000000502 dialysis Methods 0.000 description 2
239000000539 dimer Substances 0.000 description 2
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
235000019797 dipotassium phosphate Nutrition 0.000 description 2
235000021186 dishes Nutrition 0.000 description 2
238000004520 electroporation Methods 0.000 description 2
238000010828 elution Methods 0.000 description 2
210000003527 eukaryotic cell Anatomy 0.000 description 2
102000013165 exonuclease Human genes 0.000 description 2
239000000706 filtrate Substances 0.000 description 2
239000008103 glucose Substances 0.000 description 2
238000003306 harvesting Methods 0.000 description 2
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
108010018006 histidylserine Proteins 0.000 description 2
229940088597 hormone Drugs 0.000 description 2
239000005556 hormone Substances 0.000 description 2
238000002955 isolation Methods 0.000 description 2
230000002147 killing effect Effects 0.000 description 2
108010057821 leucylproline Proteins 0.000 description 2
230000002934 lysing effect Effects 0.000 description 2
239000003550 marker Substances 0.000 description 2
229920001220 nitrocellulos Polymers 0.000 description 2
108091027963 non-coding RNA Proteins 0.000 description 2
102000042567 non-coding RNA Human genes 0.000 description 2
239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
239000008057 potassium phosphate buffer Substances 0.000 description 2
239000002244 precipitate Substances 0.000 description 2
230000035755 proliferation Effects 0.000 description 2
230000006798 recombination Effects 0.000 description 2
238000005215 recombination Methods 0.000 description 2
230000008929 regeneration Effects 0.000 description 2
238000011069 regeneration method Methods 0.000 description 2
230000010076 replication Effects 0.000 description 2
-1 ribulose-1,5-bisphosphate carboxylate Chemical class 0.000 description 2
239000006152 selective media Substances 0.000 description 2
239000007787 solid Substances 0.000 description 2
238000005507 spraying Methods 0.000 description 2
108010084932 tryptophyl-proline Proteins 0.000 description 2
108010020532 tyrosyl-proline Proteins 0.000 description 2
238000001262 western blot Methods 0.000 description 2
MJRJDGKISPWEMD-OEAKJJBVSA-N (2E)-3-(3,5-dibromo-4-hydroxyphenyl)-N-[2-(3,5-dibromo-4-hydroxyphenyl)ethyl]-2-hydroxyiminopropanamide Chemical compound BrC=1C=C(C=C(C=1O)Br)CCNC(/C(/CC1=CC(=C(C(=C1)Br)O)Br)=N/O)=O MJRJDGKISPWEMD-OEAKJJBVSA-N 0.000 description 1
AUXMWYRZQPIXCC-KNIFDHDWSA-N (2s)-2-amino-4-methylpentanoic acid;(2s)-2-aminopropanoic acid Chemical compound C[C@H](N)C(O)=O.CC(C)C[C@H](N)C(O)=O AUXMWYRZQPIXCC-KNIFDHDWSA-N 0.000 description 1
NMKJJVNCRCSYDT-UHFFFAOYSA-N 1-benzylpurin-2-amine Chemical compound NC1=NC2=NC=NC2=CN1CC1=CC=CC=C1 NMKJJVNCRCSYDT-UHFFFAOYSA-N 0.000 description 1
PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
239000005971 1-naphthylacetic acid Substances 0.000 description 1
VDRACTHWBNITQP-UHFFFAOYSA-N 2-(3-methylbutyl)-7h-purin-6-amine Chemical compound CC(C)CCC1=NC(N)=C2NC=NC2=N1 VDRACTHWBNITQP-UHFFFAOYSA-N 0.000 description 1
CHZCERSEMVWNHL-UHFFFAOYSA-N 2-hydroxybenzonitrile Chemical class OC1=CC=CC=C1C#N CHZCERSEMVWNHL-UHFFFAOYSA-N 0.000 description 1
PHWAJJWKNLWZGJ-UHFFFAOYSA-N 3,5-dibromo-4-hydroxybenzoic acid Chemical compound OC(=O)C1=CC(Br)=C(O)C(Br)=C1 PHWAJJWKNLWZGJ-UHFFFAOYSA-N 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
JBVSSSZFNTXJDX-YTLHQDLWSA-N Ala-Ala-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](C)N JBVSSSZFNTXJDX-YTLHQDLWSA-N 0.000 description 1
LSLIRHLIUDVNBN-CIUDSAMLSA-N Ala-Asp-Lys Chemical compound C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(O)=O)CCCCN LSLIRHLIUDVNBN-CIUDSAMLSA-N 0.000 description 1
SOBIAADAMRHGKH-CIUDSAMLSA-N Ala-Leu-Ser Chemical compound [H]N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O SOBIAADAMRHGKH-CIUDSAMLSA-N 0.000 description 1
OEVCHROQUIVQFZ-YTLHQDLWSA-N Ala-Thr-Ala Chemical compound C[C@H](N)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@@H](C)C(O)=O OEVCHROQUIVQFZ-YTLHQDLWSA-N 0.000 description 1
244000144725 Amygdalus communis Species 0.000 description 1
244000105624 Arachis hypogaea Species 0.000 description 1
JQFZHHSQMKZLRU-IUCAKERBSA-N Arg-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](N)CCCN=C(N)N JQFZHHSQMKZLRU-IUCAKERBSA-N 0.000 description 1
PRLPSDIHSRITSF-UNQGMJICSA-N Arg-Phe-Thr Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)O)C(O)=O PRLPSDIHSRITSF-UNQGMJICSA-N 0.000 description 1
241000235349 Ascomycota Species 0.000 description 1
DONWIPDSZZJHHK-HJGDQZAQSA-N Asp-Lys-Thr Chemical compound C[C@H]([C@@H](C(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(=O)O)N)O DONWIPDSZZJHHK-HJGDQZAQSA-N 0.000 description 1
RSMZEHCMIOKNMW-GSSVUCPTSA-N Asp-Thr-Thr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H]([C@@H](C)O)C(O)=O RSMZEHCMIOKNMW-GSSVUCPTSA-N 0.000 description 1
241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
208000005623 Carcinogenesis Diseases 0.000 description 1
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
108050006400 Cyclin Proteins 0.000 description 1
102000016736 Cyclin Human genes 0.000 description 1
GEEXORWTBTUOHC-FXQIFTODSA-N Cys-Arg-Ser Chemical compound C(C[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CS)N)CN=C(N)N GEEXORWTBTUOHC-FXQIFTODSA-N 0.000 description 1
IMXSCCDUAFEIOE-UHFFFAOYSA-N D-Octopin Natural products OC(=O)C(C)NC(C(O)=O)CCCN=C(N)N IMXSCCDUAFEIOE-UHFFFAOYSA-N 0.000 description 1
IMXSCCDUAFEIOE-RITPCOANSA-N D-octopine Chemical compound [O-]C(=O)[C@@H](C)[NH2+][C@H](C([O-])=O)CCCNC(N)=[NH2+] IMXSCCDUAFEIOE-RITPCOANSA-N 0.000 description 1
238000001712 DNA sequencing Methods 0.000 description 1
108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
102000004533 Endonucleases Human genes 0.000 description 1
241000305071 Enterobacterales Species 0.000 description 1
210000000712 G cell Anatomy 0.000 description 1
XBGGUPMXALFZOT-VIFPVBQESA-N Gly-Tyr Chemical compound NCC(=O)N[C@H](C(O)=O)CC1=CC=C(O)C=C1 XBGGUPMXALFZOT-VIFPVBQESA-N 0.000 description 1
239000005562 Glyphosate Substances 0.000 description 1
241000219146 Gossypium Species 0.000 description 1
UOYGZBIPZYKGSH-SRVKXCTJSA-N His-Ser-Lys Chemical compound C1=C(NC=N1)C[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)O)N UOYGZBIPZYKGSH-SRVKXCTJSA-N 0.000 description 1
101000833492 Homo sapiens Jouberin Proteins 0.000 description 1
101000651236 Homo sapiens NCK-interacting protein with SH3 domain Proteins 0.000 description 1
206010020649 Hyperkeratosis Diseases 0.000 description 1
206010021033 Hypomenorrhoea Diseases 0.000 description 1
102100024407 Jouberin Human genes 0.000 description 1
FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
241000588748 Klebsiella Species 0.000 description 1
241000588747 Klebsiella pneumoniae Species 0.000 description 1
LHSGPCFBGJHPCY-UHFFFAOYSA-N L-leucine-L-tyrosine Natural products CC(C)CC(N)C(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 LHSGPCFBGJHPCY-UHFFFAOYSA-N 0.000 description 1
108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
MLTRLIITQPXHBJ-BQBZGAKWSA-N Leu-Asn Chemical compound CC(C)C[C@H](N)C(=O)N[C@H](C(O)=O)CC(N)=O MLTRLIITQPXHBJ-BQBZGAKWSA-N 0.000 description 1
PBGDOSARRIJMEV-DLOVCJGASA-N Leu-His-Ala Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](C)C(O)=O PBGDOSARRIJMEV-DLOVCJGASA-N 0.000 description 1
JLWZLIQRYCTYBD-IHRRRGAJSA-N Leu-Lys-Arg Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O JLWZLIQRYCTYBD-IHRRRGAJSA-N 0.000 description 1
RTIRBWJPYJYTLO-MELADBBJSA-N Leu-Lys-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@@H]1C(=O)O)N RTIRBWJPYJYTLO-MELADBBJSA-N 0.000 description 1
QMKFDEUJGYNFMC-AVGNSLFASA-N Leu-Pro-Arg Chemical compound CC(C)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCN=C(N)N)C(O)=O QMKFDEUJGYNFMC-AVGNSLFASA-N 0.000 description 1
FGZVGOAAROXFAB-IXOXFDKPSA-N Leu-Thr-His Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)NC(=O)[C@H](CC(C)C)N)O FGZVGOAAROXFAB-IXOXFDKPSA-N 0.000 description 1
GDBQQVLCIARPGH-UHFFFAOYSA-N Leupeptin Natural products CC(C)CC(NC(C)=O)C(=O)NC(CC(C)C)C(=O)NC(C=O)CCCN=C(N)N GDBQQVLCIARPGH-UHFFFAOYSA-N 0.000 description 1
102000003960 Ligases Human genes 0.000 description 1
108090000364 Ligases Proteins 0.000 description 1
FZIJIFCXUCZHOL-CIUDSAMLSA-N Lys-Ala-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCCCN FZIJIFCXUCZHOL-CIUDSAMLSA-N 0.000 description 1
NPBGTPKLVJEOBE-IUCAKERBSA-N Lys-Arg Chemical compound NCCCC[C@H](N)C(=O)N[C@H](C(O)=O)CCCNC(N)=N NPBGTPKLVJEOBE-IUCAKERBSA-N 0.000 description 1
ATIPDCIQTUXABX-UWVGGRQHSA-N Lys-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](N)CCCCN ATIPDCIQTUXABX-UWVGGRQHSA-N 0.000 description 1
XFANQCRHTMOEAP-WDSOQIARSA-N Lys-Pro-Trp Chemical compound [H]N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(O)=O XFANQCRHTMOEAP-WDSOQIARSA-N 0.000 description 1
XDGFFEZAZHRZFR-RHYQMDGZSA-N Met-Leu-Thr Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(O)=O XDGFFEZAZHRZFR-RHYQMDGZSA-N 0.000 description 1
WEDDFMCSUNNZJR-WDSKDSINSA-N Met-Ser Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CO)C(O)=O WEDDFMCSUNNZJR-WDSKDSINSA-N 0.000 description 1
KYJHWKAMFISDJE-RCWTZXSCSA-N Met-Thr-Met Chemical compound CSCC[C@H](N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@H](C(O)=O)CCSC KYJHWKAMFISDJE-RCWTZXSCSA-N 0.000 description 1
102000016943 Muramidase Human genes 0.000 description 1
108010014251 Muramidase Proteins 0.000 description 1
108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
YBAFDPFAUTYYRW-UHFFFAOYSA-N N-L-alpha-glutamyl-L-leucine Natural products CC(C)CC(C(O)=O)NC(=O)C(N)CCC(O)=O YBAFDPFAUTYYRW-UHFFFAOYSA-N 0.000 description 1
101710163270 Nuclease Proteins 0.000 description 1
241000283973 Oryctolagus cuniculus Species 0.000 description 1
240000007594 Oryza sativa Species 0.000 description 1
235000007164 Oryza sativa Nutrition 0.000 description 1
240000007377 Petunia x hybrida Species 0.000 description 1
101710163504 Phaseolin Proteins 0.000 description 1
ACJULKNZOCRWEI-ULQDDVLXSA-N Phe-Met-Leu Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(C)C)C(O)=O ACJULKNZOCRWEI-ULQDDVLXSA-N 0.000 description 1
206010035664 Pneumonia Diseases 0.000 description 1
FELJDCNGZFDUNR-WDSKDSINSA-N Pro-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@@H]1CCCN1 FELJDCNGZFDUNR-WDSKDSINSA-N 0.000 description 1
108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
VBKBDLMWICBSCY-IMJSIDKUSA-N Ser-Asp Chemical compound OC[C@H](N)C(=O)N[C@H](C(O)=O)CC(O)=O VBKBDLMWICBSCY-IMJSIDKUSA-N 0.000 description 1
BGOWRLSWJCVYAQ-CIUDSAMLSA-N Ser-Asp-Leu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(O)=O BGOWRLSWJCVYAQ-CIUDSAMLSA-N 0.000 description 1
YUJLIIRMIAGMCQ-CIUDSAMLSA-N Ser-Leu-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(O)=O YUJLIIRMIAGMCQ-CIUDSAMLSA-N 0.000 description 1
BSXKBOUZDAZXHE-CIUDSAMLSA-N Ser-Pro-Glu Chemical compound [H]N[C@@H](CO)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(O)=O BSXKBOUZDAZXHE-CIUDSAMLSA-N 0.000 description 1
LDEBVRIURYMKQS-WISUUJSJSA-N Ser-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H](N)CO LDEBVRIURYMKQS-WISUUJSJSA-N 0.000 description 1
108020004682 Single-Stranded DNA Proteins 0.000 description 1
244000061456 Solanum tuberosum Species 0.000 description 1
235000002595 Solanum tuberosum Nutrition 0.000 description 1
235000021536 Sugar beet Nutrition 0.000 description 1
108700026226 TATA Box Proteins 0.000 description 1
NJEMRSFGDNECGF-GCJQMDKQSA-N Thr-Ala-Asp Chemical compound C[C@@H](O)[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@H](C(O)=O)CC(O)=O NJEMRSFGDNECGF-GCJQMDKQSA-N 0.000 description 1
UTSWGQNAQRIHAI-UNQGMJICSA-N Thr-Arg-Phe Chemical compound NC(N)=NCCC[C@H](NC(=O)[C@@H](N)[C@H](O)C)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 UTSWGQNAQRIHAI-UNQGMJICSA-N 0.000 description 1
VUSAEKOXGNEYNE-PBCZWWQYSA-N Thr-His-Asn Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](CC1=CN=CN1)C(=O)N[C@@H](CC(=O)N)C(=O)O)N)O VUSAEKOXGNEYNE-PBCZWWQYSA-N 0.000 description 1
KERCOYANYUPLHJ-XGEHTFHBSA-N Thr-Pro-Ser Chemical compound C[C@@H](O)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(O)=O KERCOYANYUPLHJ-XGEHTFHBSA-N 0.000 description 1
VTFWAGGJDRSQFG-MELADBBJSA-N Tyr-Asn-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CC(=O)N)NC(=O)[C@H](CC2=CC=C(C=C2)O)N)C(=O)O VTFWAGGJDRSQFG-MELADBBJSA-N 0.000 description 1
SYFHQHYTNCQCCN-MELADBBJSA-N Tyr-Ser-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CO)NC(=O)[C@H](CC2=CC=C(C=C2)O)N)C(=O)O SYFHQHYTNCQCCN-MELADBBJSA-N 0.000 description 1
108020005202 Viral DNA Proteins 0.000 description 1
241000269370 Xenopus <genus> Species 0.000 description 1
241000607479 Yersinia pestis Species 0.000 description 1
240000008042 Zea mays Species 0.000 description 1
235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
235000002017 Zea mays subsp mays Nutrition 0.000 description 1
239000004480 active ingredient Substances 0.000 description 1
239000000853 adhesive Substances 0.000 description 1
230000001070 adhesive effect Effects 0.000 description 1
230000002411 adverse Effects 0.000 description 1
238000001042 affinity chromatography Methods 0.000 description 1
108010050625 agropine synthase Proteins 0.000 description 1
108010017893 alanyl-alanyl-alanine Proteins 0.000 description 1
ZVDPYSVOZFINEE-BQBZGAKWSA-N alpha-Asp-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@@H](N)CC(O)=O ZVDPYSVOZFINEE-BQBZGAKWSA-N 0.000 description 1
229960000723 ampicillin Drugs 0.000 description 1
AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
101150009700 anp32b gene Proteins 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
239000000427 antigen Substances 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
238000013459 approach Methods 0.000 description 1
108010013835 arginine glutamate Proteins 0.000 description 1
101150037081 aroA gene Proteins 0.000 description 1
108010047857 aspartylglycine Proteins 0.000 description 1
108010092854 aspartyllysine Proteins 0.000 description 1
238000003556 assay Methods 0.000 description 1
244000052616 bacterial pathogen Species 0.000 description 1
239000011324 bead Substances 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
229910052794 bromium Inorganic materials 0.000 description 1
230000036952 cancer formation Effects 0.000 description 1
229940041514 candida albicans extract Drugs 0.000 description 1
125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
231100000504 carcinogenesis Toxicity 0.000 description 1
241001233037 catfish Species 0.000 description 1
210000003855 cell nucleus Anatomy 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
239000001913 cellulose Substances 0.000 description 1
229920002678 cellulose Polymers 0.000 description 1
235000013339 cereals Nutrition 0.000 description 1
230000008859 change Effects 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
238000011098 chromatofocusing Methods 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
239000013611 chromosomal DNA Substances 0.000 description 1
239000004927 clay Substances 0.000 description 1
238000004140 cleaning Methods 0.000 description 1
239000013599 cloning vector Substances 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
238000011109 contamination Methods 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
244000038559 crop plants Species 0.000 description 1
229940127089 cytotoxic agent Drugs 0.000 description 1
239000002254 cytotoxic agent Substances 0.000 description 1
231100000599 cytotoxic agent Toxicity 0.000 description 1
239000008380 degradant Substances 0.000 description 1
238000000432 density-gradient centrifugation Methods 0.000 description 1
239000003599 detergent Substances 0.000 description 1
238000002405 diagnostic procedure Methods 0.000 description 1
229960005215 dichloroacetic acid Drugs 0.000 description 1
230000004069 differentiation Effects 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
238000001035 drying Methods 0.000 description 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
229960005542 ethidium bromide Drugs 0.000 description 1
108010092809 exonuclease Bal 31 Proteins 0.000 description 1
239000003337 fertilizer Substances 0.000 description 1
230000009969 flowable effect Effects 0.000 description 1
238000005194 fractionation Methods 0.000 description 1
125000000524 functional group Chemical group 0.000 description 1
XBGGUPMXALFZOT-UHFFFAOYSA-N glycyl-L-tyrosine hemihydrate Natural products NCC(=O)NC(C(O)=O)CC1=CC=C(O)C=C1 XBGGUPMXALFZOT-UHFFFAOYSA-N 0.000 description 1
108010087823 glycyltyrosine Proteins 0.000 description 1
XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 1
229940097068 glyphosate Drugs 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
238000004128 high performance liquid chromatography Methods 0.000 description 1
239000012535 impurity Substances 0.000 description 1
238000000338 in vitro Methods 0.000 description 1
238000001727 in vivo Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
230000006698 induction Effects 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
238000002347 injection Methods 0.000 description 1
239000007924 injection Substances 0.000 description 1
CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
229960000367 inositol Drugs 0.000 description 1
238000003780 insertion Methods 0.000 description 1
230000037431 insertion Effects 0.000 description 1
QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
229960001669 kinetin Drugs 0.000 description 1
231100000518 lethal Toxicity 0.000 description 1
230000001665 lethal effect Effects 0.000 description 1
108010012058 leucyltyrosine Proteins 0.000 description 1
GDBQQVLCIARPGH-ULQDDVLXSA-N leupeptin Chemical compound CC(C)C[C@H](NC(C)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C=O)CCCN=C(N)N GDBQQVLCIARPGH-ULQDDVLXSA-N 0.000 description 1
108010052968 leupeptin Proteins 0.000 description 1
230000004807 localization Effects 0.000 description 1
229960000274 lysozyme Drugs 0.000 description 1
239000004325 lysozyme Substances 0.000 description 1
235000010335 lysozyme Nutrition 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
235000009973 maize Nutrition 0.000 description 1
238000013507 mapping Methods 0.000 description 1
239000000463 material Substances 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
238000000520 microinjection Methods 0.000 description 1
239000006151 minimal media Substances 0.000 description 1
230000001483 mobilizing effect Effects 0.000 description 1
238000012986 modification Methods 0.000 description 1
230000004048 modification Effects 0.000 description 1
229910000069 nitrogen hydride Inorganic materials 0.000 description 1
108010058731 nopaline synthase Proteins 0.000 description 1
235000015097 nutrients Nutrition 0.000 description 1
210000000287 oocyte Anatomy 0.000 description 1
238000004806 packaging method and process Methods 0.000 description 1
239000012188 paraffin wax Substances 0.000 description 1
235000020232 peanut Nutrition 0.000 description 1
210000001322 periplasm Anatomy 0.000 description 1
239000000575 pesticide Substances 0.000 description 1
LWTDZKXXJRRKDG-UHFFFAOYSA-N phaseollin Natural products C1OC2=CC(O)=CC=C2C2C1C1=CC=C3OC(C)(C)C=CC3=C1O2 LWTDZKXXJRRKDG-UHFFFAOYSA-N 0.000 description 1
239000013600 plasmid vector Substances 0.000 description 1
239000001267 polyvinylpyrrolidone Substances 0.000 description 1
229920000036 polyvinylpyrrolidone Polymers 0.000 description 1
235000013855 polyvinylpyrrolidone Nutrition 0.000 description 1
235000012015 potatoes Nutrition 0.000 description 1
239000000843 powder Substances 0.000 description 1
230000001376 precipitating effect Effects 0.000 description 1
210000001236 prokaryotic cell Anatomy 0.000 description 1
239000011541 reaction mixture Substances 0.000 description 1
230000002829 reductive effect Effects 0.000 description 1
230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
230000003362 replicative effect Effects 0.000 description 1
235000009566 rice Nutrition 0.000 description 1
238000006798 ring closing metathesis reaction Methods 0.000 description 1
CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
230000028327 secretion Effects 0.000 description 1
238000000926 separation method Methods 0.000 description 1
238000012163 sequencing technique Methods 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
238000004904 shortening Methods 0.000 description 1
239000013605 shuttle vector Substances 0.000 description 1
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
238000010183 spectrum analysis Methods 0.000 description 1
230000002269 spontaneous effect Effects 0.000 description 1
239000007921 spray Substances 0.000 description 1
239000003381 stabilizer Substances 0.000 description 1
238000010186 staining Methods 0.000 description 1
238000010561 standard procedure Methods 0.000 description 1
239000007858 starting material Substances 0.000 description 1
238000003860 storage Methods 0.000 description 1
125000001424 substituent group Chemical group 0.000 description 1
KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 1
239000000725 suspension Substances 0.000 description 1
108010061238 threonyl-glycine Proteins 0.000 description 1
235000013619 trace mineral Nutrition 0.000 description 1
239000011573 trace mineral Substances 0.000 description 1
238000010361 transduction Methods 0.000 description 1
230000026683 transduction Effects 0.000 description 1
238000001890 transfection Methods 0.000 description 1
238000000844 transformation Methods 0.000 description 1
238000013519 translation Methods 0.000 description 1
238000005199 ultracentrifugation Methods 0.000 description 1
230000003612 virological effect Effects 0.000 description 1
230000001018 virulence Effects 0.000 description 1
238000005406 washing Methods 0.000 description 1
239000000080 wetting agent Substances 0.000 description 1
239000002023 wood Substances 0.000 description 1
239000012138 yeast extract Substances 0.000 description 1
DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Organic Chemistry (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Cell Biology (AREA)
Medicinal Chemistry (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

DD88330727A 1987-07-08 1988-07-08 Verfahren zur herstellung einer pflanzenzelle bzw. eines pflanzenteils oder einer pflanze mit einer derartigen pflanzenzelle DD284048A5 (de)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US07/071,146 US4810648A (en)	1986-01-08	1987-07-08	Haloarylnitrile degrading gene, its use, and cells containing the gene

Publications (1)

Publication Number	Publication Date
DD284048A5 true DD284048A5 (de)	1990-10-31

Family

ID=22099529

Family Applications (2)

Application Number	Title	Priority Date	Filing Date
DD88330727A DD284048A5 (de)	1987-07-08	1988-07-08	Verfahren zur herstellung einer pflanzenzelle bzw. eines pflanzenteils oder einer pflanze mit einer derartigen pflanzenzelle
DD88317745A DD285922A5 (de)	1987-07-08	1988-07-08	Von bakterienzellbestandteilen praktisch freie zusammensetzung fuer den pflanzenschutz und verfahren zur gewinnung des wirkstoffes fuer diese

Family Applications After (1)

Application Number	Title	Priority Date	Filing Date
DD88317745A DD285922A5 (de)	1987-07-08	1988-07-08	Von bakterienzellbestandteilen praktisch freie zusammensetzung fuer den pflanzenschutz und verfahren zur gewinnung des wirkstoffes fuer diese

Country Status (15)

Country	Link
US (1)	US4810648A (da)
EP (1)	EP0373173A1 (da)
KR (1)	KR890701735A (da)
CN (1)	CN1031252A (da)
AU (1)	AU2071988A (da)
BR (1)	BR8807609A (da)
CA (1)	CA1339683C (da)
DD (2)	DD284048A5 (da)
DK (1)	DK2890A (da)
ES (1)	ES2010763A6 (da)
GR (1)	GR880100421A (da)
IL (1)	IL87001A (da)
PL (1)	PL273589A1 (da)
WO (1)	WO1989000193A1 (da)
ZA (1)	ZA884894B (da)

Families Citing this family (349)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5614395A (en) *	1988-03-08	1997-03-25	Ciba-Geigy Corporation	Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
FR2629098B1 (fr) *	1988-03-23	1990-08-10	Rhone Poulenc Agrochimie	Gene chimerique de resistance herbicide
US5347076A (en) *	1988-05-19	1994-09-13	Schering Aktiengesellschaft	Herbicide-tolerant plants expressing carbamate hydrolase
US5369022A (en) *	1988-12-30	1994-11-29	American Cyanamid Company	Method to improve the protection of crops from herbicidal injury
CA2018884A1 (en) *	1989-06-22	1990-12-22	Irving Gordon	Plant transformation construct
IE912716A1 (en) *	1990-08-14	1992-02-26	Res Dev Foundation	Protein Structure of the Plant Toxin Gelonin
US5629190A (en) *	1992-08-10	1997-05-13	Rhone-Poulenc Chimie	Polypeptides possessing a nitrilase activity and method of converting nitriles to carboxylates by means of said polypeptides
FR2694571B1 (fr) *	1992-08-10	1994-11-04	Rhone Poulenc Chimie	Polypeptides possédant une activité nitrilase, séquence d'ADN codant pour lesdits polypeptides, cassettes d'expression et micro-organismes hôtes permettant leur obtention.
US5695928A (en)	1993-12-10	1997-12-09	Novartis Corporation	Rapid immunoassay for detection of antibodies or antigens incorporating simultaneous sample extraction and immunogenic reaction
DE69526377T2 (de) *	1994-12-09	2002-11-07	Microscience Ltd	Virulenzgene in der VGC2-Region von Salmonella
US5863750A (en) *	1996-12-18	1999-01-26	Cytec Tech Corp	Methods for the detoxification of nitrile and/or amide compounds
US6060265A (en) *	1996-12-18	2000-05-09	Cytec Technology Corporation	Methods for the detoxification of nitrile and/or amide compounds
GB9712370D0 (en) *	1997-06-14	1997-08-13	Aepact Ltd	Therapeutic systems
US7161064B2 (en) *	1997-08-12	2007-01-09	North Carolina State University	Method for producing stably transformed duckweed using microprojectile bombardment
US6492578B1 (en) *	1998-07-10	2002-12-10	Calgene Llc	Expression of herbicide tolerance genes in plant plastids
US7057090B1 (en) *	1998-07-17	2006-06-06	Rutgers, The State University Of New Jersey	Agrobacterium-mediated transformation of turfgrass
EP1098968A4 (en) *	1998-07-20	2002-01-02	Univ Jefferson	NITRILASE APPROVALS
ATE486940T1 (de) *	1998-09-04	2010-11-15	Emergent Product Dev Uk Ltd	Abgeschwächte salmonella sp12 mutante als antigen-träger.
US6515206B1 (en)	1998-12-23	2003-02-04	Calgene Llc	Plastid transformation of Brassica
GB9910812D0 (en) *	1999-05-10	1999-07-07	Microscience Ltd	Vaccine composition
US6870038B2 (en)	2000-03-31	2005-03-22	E. I. Du Pont De Nemours And Company	Isolation and expression of a gene for a nitrilase from Acidovorax facilis 72W
GB0008419D0 (en) *	2000-04-05	2000-05-24	Bioinvent Int Ab	A method for invitro molecular evolution of antibody function
GB0008966D0 (en) *	2000-04-13	2000-05-31	Imp College Innovations Ltd	Vectors for gene therapy
EP1320594A2 (en)	2000-09-30	2003-06-25	E.I. Du Pont De Nemours And Company	Exbb-exbd-tonb gene cluster of pseudomonas putida dot-t1e conferring tolerance to aromatic compounds and resistance to antibiotics
DE60238904D1 (de) *	2001-02-22	2011-02-24	Biogemma Fr	Konstitutiver promotor aus arabidopsis
US7977046B2 (en) *	2002-05-10	2011-07-12	Monsanto Technology Llc	Method of selecting DNA constructs for herbicide tolerance in plants
AU2002315526A1 (en)	2001-07-06	2003-01-21	Monsanto Technology Llc	Methods for enhancing segregation of transgenes in plants and compositions thereof
RU2320722C2 (ru)	2002-02-26	2008-03-27	Зингента Лимитед	Способ избирательного получения растений с мужской или женской стерильностью
US6948972B2 (en) *	2002-03-27	2005-09-27	Pent Technologies, Inc.	Overhead lighting splitter
CN101508996B (zh)	2002-07-18	2012-01-11	孟山都技术有限公司	利用人工的多核苷酸及其组合物来减少转基因沉默的方法
GB0217033D0 (en)	2002-07-23	2002-08-28	Delta Biotechnology Ltd	Gene and polypeptide sequences
US7994400B2 (en) *	2002-08-16	2011-08-09	Royster-Clark Resources, Llc	Plant seed mixtures
MXPA05003003A (es) *	2002-09-17	2005-11-23	Ceres Inc	Sistema de contencion biologico.
US7476777B2 (en) *	2002-09-17	2009-01-13	Ceres, Inc.	Biological containment system
GB0226105D0 (en) *	2002-11-08	2002-12-18	St Georges S Entpr Ltd	Pain relief agents
WO2004071168A2 (en) *	2003-02-05	2004-08-26	Divergence, Inc.	Nucleic acids encoding anthelmintic agents and plants made therefrom
GB0304993D0 (en) *	2003-03-05	2003-04-09	Univ Nottingham Trent	Novel screening method
US7880061B2 (en)	2003-02-25	2011-02-01	The Curators Of The University Of Missouri	Soybean variety SN97-6946
CA2519912A1 (en)	2003-03-28	2004-10-14	Monsanto Technology Llc	Regulatory regions that promote early plant seed enhanced transcription
PT1658364T (pt)	2003-08-25	2017-07-11	Monsanto Technology Llc	Elementos reguladores da tubulina para utilização em plantas
US7807874B2 (en)	2003-12-10	2010-10-05	Monsanto Technology Llc	Stress tolerant plants and methods thereof
US8420087B2 (en) *	2004-01-05	2013-04-16	Antisoma Research Limited	Interleukin-12 targeted to oncofoetal fibronectin
US7371848B2 (en)	2004-01-20	2008-05-13	Monsanto Technology Llc	Chimeric promoters comprising a caulimovirus promoter enhancer for use in plants
US7368629B2 (en) *	2004-02-04	2008-05-06	Divergence, Inc.	Nucleic acids encoding anthelmintic agents and plants made therefrom
EP1788861B1 (en)	2004-08-24	2017-04-12	Monsanto Technology, LLC	Adenylate translocator protein gene non-coding regulatory elements for use in plants
GB0420091D0 (en) *	2004-09-10	2004-10-13	Univ Nottingham Trent	Medical implant materials
CN101056975A (zh)	2004-09-14	2007-10-17	孟山都技术有限公司	用于植物的启动子分子
CA2595171C (en)	2004-12-21	2015-03-17	Monsanto Technology Llc	Transgenic plants with enhanced agronomic traits
ES2459369T3 (es)	2004-12-21	2014-05-09	Monsanto Technology Llc	Plantas transgénicas con rasgos agronómicos potenciados
EP1855724A2 (en) *	2005-02-01	2007-11-21	Research Development Foundation	Blys fusion proteins for targeting blys receptor and methods for treatment of b-cell proliferative disorders
US20060193849A1 (en) *	2005-02-25	2006-08-31	Antisoma Plc	Biological materials and uses thereof
WO2006119318A2 (en)	2005-05-02	2006-11-09	Purdue Research Foundation	Methods for increasing the yield of fermentable sugars from plant stover
GB0514661D0 (en) *	2005-07-16	2005-08-24	Medical Res Council	Methods
WO2007015945A2 (en)	2005-07-29	2007-02-08	Monsanto Technology Llc	Development of novel germplasm using segregates from transgenic crosses
DK1806359T3 (da)	2005-09-05	2010-06-14	Immatics Biotechnologies Gmbh	Tumorassocierede peptider, der bindes promiskuøst til Humant Leukocyt-Antigen (HLA) klasse II molekyler
PT1760089E (pt)	2005-09-05	2009-10-19	Immatics Biotechnologies Gmbh	Peptídeos associados a tumores que se ligam a moléculas de classe i ou ii do antigénio leucocitário humano (hla) e correspondente vacina anti-cancerígena
US8993846B2 (en)	2005-09-06	2015-03-31	Monsanto Technology Llc	Vectors and methods for improved plant transformation efficiency
GB0519398D0 (en) *	2005-09-23	2005-11-02	Antisoma Plc	Biological materials and uses thereof
UA102367C2 (ru)	2005-10-03	2013-07-10	Монсанто Текнолоджи Ллс	Семя трансгенной кукурузы с повышенным содержанием лизина
CA2625031C (en)	2005-10-13	2016-07-19	Monsanto Technology Llc	Methods for producing hybrid seed
EP1962577A4 (en)	2005-12-21	2009-12-16	Monsanto Technology Llc	Transgenic plants with enhanced agronomic traits
US7868228B2 (en)	2006-01-31	2011-01-11	Monsanto Technology Llc	Phosphopantetheinyl transferases from bacteria
BRPI0708480A2 (pt) *	2006-03-02	2011-05-31	Athenix Corp	processos e composições para aperfeiçoada atividade de enzima em plantas transgênicas
US7820883B2 (en) *	2006-03-15	2010-10-26	Dow Agrosciences Llc	Resistance to auxinic herbicides
GB0607354D0 (en)	2006-04-12	2006-05-24	Bioinvent Int Ab	Agent, Composition And Method
GB0607798D0 (en) *	2006-04-20	2006-05-31	Alligator Bioscience Ab	Novel polypeptides and use thereof
WO2007134122A2 (en)	2006-05-09	2007-11-22	The Curators Of The University Of Missouri	Plant artificial chromosome platforms via telomere truncation
WO2007137075A2 (en)	2006-05-16	2007-11-29	Monsanto Technology Llc	Use of non-agrobacterium bacterial species for plant transformation
CN101657720A (zh) *	2006-05-31	2010-02-24	荷兰联合利华有限公司	筛选改变皮肤和/或头发色素沉着的化合物的方法
WO2008021543A2 (en)	2006-08-17	2008-02-21	Monsanto Technology, Llc	Transgenic plants with enhanced agronomic traits
US7939721B2 (en)	2006-10-25	2011-05-10	Monsanto Technology Llc	Cropping systems for managing weeds
EP2115150A4 (en)	2006-12-15	2010-06-02	Agrinomics Llc	GENERATION OF PLANTS WITH CHANGED OIL, PROTEIN OR FIBER CONTENT
US7777102B2 (en) *	2007-02-08	2010-08-17	University Of Tennessee Research Foundation	Soybean varieties
BRPI0807573A2 (pt) *	2007-02-23	2014-07-01	Univ Georgia	Composições e métodos úteis para recombinação sítio-dirigida em plantas.
EP2118290B1 (en)	2007-03-09	2013-05-08	Monsanto Technology, LLC	Method of meristem excision and transformation
US7723582B2 (en) *	2007-04-12	2010-05-25	Dow Agrosciences Llc	Canola cultivar DN041100
US7723579B2 (en) *	2007-04-12	2010-05-25	Dow Agrosciences Llc	Canola cultivar DN040244
US7718852B2 (en) *	2007-04-12	2010-05-18	Dow Agrosciences Llc	Canola cultivar DN040241
US7723581B2 (en) *	2007-04-12	2010-05-25	Dow Agrosciences Llc	Canola cultivar DN040845
US7728195B2 (en) *	2007-04-12	2010-06-01	Dow Agrosciences Llc	Canola cultivar DN040856
AU2008240142B2 (en)	2007-04-12	2014-11-06	Corteva Agriscience Llc	Novel canola cultivars having high yield and stabilized fatty acid profiles
US7723577B2 (en) *	2007-04-12	2010-05-25	Dow Agrosciences Llc	Canola cultivar DN040847
US7723578B2 (en) *	2007-04-12	2010-05-25	Dow Agrosciences Llc	Canola cultivar DN040839
US7723580B2 (en) *	2007-04-12	2010-05-25	Dow Agrosciences Llc	Canola cultivar DN040844
EP3567113A1 (en)	2007-06-06	2019-11-13	Monsanto Technology LLC	Genes and uses for plant enhancement
US8642847B2 (en) *	2007-06-21	2014-02-04	Lee K. French	Corn inbreds like FAR601 and hybrids thereof
US8945653B2 (en) *	2007-06-21	2015-02-03	Suntava, Llc	Extracted whole corn kernels and improved processed and processable corn produced thereby
US20110265221A1 (en)	2007-07-10	2011-10-27	Monsanto Technology Llc	Transgenic plants with enhanced agronomic traits
PL3042914T3 (pl)	2007-07-27	2019-01-31	Immatics Biotechnologies Gmbh	Nowe immunogenne epitopy do immunoterapii
JP6008461B2 (ja)	2007-07-27	2016-10-19	イマティクスバイオテクノロジーズゲーエムベーハー	神経細胞性脳腫瘍に対する新規免疫療法
EP2195438B1 (en)	2007-10-05	2013-01-23	Dow AgroSciences LLC	Methods for transferring molecular substances into plant cells
ES2688721T3 (es)	2007-11-01	2018-11-06	Astellas Pharma Inc.	Polipéptidos y ácidos nucleicos inmunosupresores
US8097712B2 (en)	2007-11-07	2012-01-17	Beelogics Inc.	Compositions for conferring tolerance to viral disease in social insects, and the use thereof
EP2215473A1 (en) *	2007-11-27	2010-08-11	Unilever Plc, A Company Registered In England And Wales under company no. 41424 of Unilever House	Screening methods
DK2219469T3 (da)	2007-12-03	2017-11-20	Syngenta Participations Ag	Modificering af enzymatisk disponerede proteiner
EP2586293A1 (en)	2007-12-20	2013-05-01	Dow Agrosciences LLC	Low saturated-fat sunflower and associated methods
DK2105501T3 (da)	2008-03-27	2015-11-23	Immatics Biotechnologies Gmbh	Hidtil ukendt immunterapi mod neuronale tumorer og hjernetumorer
CN103320442B (zh)	2008-04-07	2016-05-25	孟山都技术公司	植物调控元件及其应用
EP2271760A2 (en)	2008-04-29	2011-01-12	Monsanto Technology LLC	Genes and uses for plant enhancement
US7947877B2 (en) *	2008-05-14	2011-05-24	Monosanto Technology LLC	Plants and seeds of spring canola variety SCV328921
US8829282B2 (en) *	2008-05-14	2014-09-09	Monsanto Technology, Llc	Plants and seeds of spring canola variety SCV425044
NO2119726T3 (da)	2008-05-14	2015-05-23
US7964774B2 (en) *	2008-05-14	2011-06-21	Monsanto Technology Llc	Plants and seeds of spring canola variety SCV384196
US7935870B2 (en) *	2008-05-14	2011-05-03	Monsanto Technology Llc	Plants and seeds of spring canola variety SCV354718
EP2326344A4 (en)	2008-06-16	2013-08-07	Prokarium Ltd	VACCINES WITH SALMONELLA VECTOR AGAINST CHLAMYDIA AND METHOD OF ADMINISTRATION
EP3023499A1 (en)	2008-07-16	2016-05-25	Monsanto Technology LLC	Methods and vectors for producing transgenic plants
EP2172211B1 (en)	2008-10-01	2014-12-03	Immatics Biotechnologies GmbH	Composition of tumor-associated peptides and related anti-cancer vaccine for the treatment of glioblastoma (GBM) and other cancers
CA2737595C (en)	2008-10-14	2017-09-12	Monsanto Technology Llc	Utilization of fatty acid desaturases from hemiselmis spp.
EP2362723A1 (en) *	2008-11-04	2011-09-07	Dow Agrosciences LLC	Omega-9 quality brassica juncea
GB0905790D0 (en)	2009-04-03	2009-05-20	Alligator Bioscience Ab	Novel polypeptides and use thereof
US20110239315A1 (en)	2009-01-12	2011-09-29	Ulla Bonas	Modular dna-binding domains and methods of use
EP2206723A1 (en)	2009-01-12	2010-07-14	Bonas, Ulla	Modular DNA-binding domains
US8373025B2 (en)	2009-02-09	2013-02-12	Chromatin Germplasm, Llc	Herbicide resistant sorghum
US8304616B2 (en) *	2009-04-07	2012-11-06	University Of Georgia Research Foundation, Inc.	Soybean variety G00-3209
US8071864B2 (en) *	2009-04-15	2011-12-06	Monsanto Technology Llc	Plants and seeds of corn variety CV897903
US8362332B2 (en) *	2009-04-15	2013-01-29	Monsanto Technology Llc	Plants and seeds of corn variety CV165560
US8071865B2 (en) *	2009-04-15	2011-12-06	Monsanto Technology Llc	Plants and seeds of corn variety CV589782
US20100299773A1 (en) *	2009-05-20	2010-11-25	Monsanto Technology Llc	Methods and compositions for selecting an improved plant
EP2437592A4 (en)	2009-06-05	2013-02-27	Univ Florida	ISOLATION AND TARGETED SUPPRESSION OF BIOSYNTHETIC LIGNIN GENES FROM SUGAR TUBE
US8466342B2 (en)	2009-06-09	2013-06-18	Pioneer Hi Bred International Inc	Early endosperm promoter and methods of use
US8071848B2 (en) *	2009-06-17	2011-12-06	Monsanto Technology Llc	Plants and seeds of spring canola variety SCV218328
WO2011034704A1 (en)	2009-09-17	2011-03-24	Monsanto Technology Llc	Soybean transgenic event mon 87708 and methods of use thereof
US8440891B2 (en)	2009-09-22	2013-05-14	Board of Trustees of the University of Akransas, N.A.	Rice cultivar CL 142-AR
US8962584B2 (en)	2009-10-14	2015-02-24	Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd.	Compositions for controlling Varroa mites in bees
US8440892B2 (en)	2009-10-15	2013-05-14	Board Of Trustees Of The University Of Arkansas, N.A.	Rice cultivar CL 181-AR
IN2012DN02333A (da) *	2009-10-16	2015-08-21	Dow Agrosciences Llc
WO2011050271A1 (en)	2009-10-23	2011-04-28	Monsanto Technology Llc	Methods and compositions for expression of transgenes in plants
MX2012004819A (es)	2009-10-26	2012-06-25	Pioneer Hi Bred Int	Promotor especifico de ovulo somatico y metodos de uso.
TW201142029A (en)	2009-11-24	2011-12-01	Univ Leuven Kath	Banana promoters
GB0920891D0 (en)	2009-11-27	2010-01-13	Syngenta Participations Ag	Herbicidal compositions
DK2816112T3 (da)	2009-12-10	2018-11-19	Univ Minnesota	TAL-effektor-medieret DNA-modifikation
DK2513146T3 (da)	2009-12-18	2017-07-31	Kancera Ab	Antistoffer mod ror1, der er i stand til at fremkalde celledød af cll
BR122021001267B1 (pt)	2010-01-14	2022-03-15	Monsanto Technology Llc	Molécula de dna compreendendo elementos reguladores de plantas
US8138394B2 (en) *	2010-02-26	2012-03-20	Monsanto Technology Llc	Plants and seeds of spring canola variety SCV431158
US8148611B2 (en) *	2010-02-26	2012-04-03	Monsanto Technology Llc	Plants and seeds of spring canola variety SCV453784
US8143488B2 (en) *	2010-02-26	2012-03-27	Monsanto Technoloy LLC	Plants and seeds of spring canola variety SCV470336
MY163887A (en)	2010-03-08	2017-11-15	Monsanto Technology Llc	Polynucleotide molecules for gene regulations in plants
US8581048B2 (en) *	2010-03-09	2013-11-12	Monsanto Technology, Llc	Plants and seeds of spring canola variety SCV119103
US8153865B2 (en) *	2010-03-11	2012-04-10	Monsanto Technology Llc	Plants and seeds of spring canola variety SCV152154
GB201019331D0 (en)	2010-03-19	2010-12-29	Immatics Biotechnologies Gmbh	Methods for the diagnosis and treatment of cancer based on AVL9
GB201004551D0 (en)	2010-03-19	2010-05-05	Immatics Biotechnologies Gmbh	NOvel immunotherapy against several tumors including gastrointestinal and gastric cancer
EP2552191B1 (en)	2010-03-31	2020-11-04	Dow AgroSciences, LLC	Plant peptide gamma-zein for delivery of biomolecules into plant cells
WO2011146121A1 (en)	2010-05-17	2011-11-24	Sangamo Biosciences, Inc.	Novel dna-binding proteins and uses thereof
AU2011274811C1 (en)	2010-07-07	2015-09-24	Corteva Agriscience Llc	Linear DNA molecule delivery using pegylated quantum dots for stable transformation in plants
US9222100B2 (en)	2010-08-24	2015-12-29	Monsanto Technology Llc	Methods and DNA constructs for autoregulating transgene silencing
PT2611925T (pt)	2010-08-30	2018-03-02	Dow Agrosciences Llc	Intensificador do vírus baciliforme da cana sacarina (scbv) e seu uso em genómica funcional de plantas
CA2820706C (en)	2010-12-03	2018-05-22	Ms Technologies, Llc	Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
AR083029A1 (es)	2010-12-09	2013-01-23	Syngenta Participations Ag	Metodos y composiciones que utilizan arn interferente pequeño (arnip) para el control de nematodos en plantas
GB201020995D0 (en)	2010-12-10	2011-01-26	Bioinvent Int Ab	Biological materials and uses thereof
TWI667347B (zh)	2010-12-15	2019-08-01	瑞士商先正達合夥公司	大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法
KR20210000752A (ko)	2011-02-22	2021-01-05	애그리제네틱스, 인크.	오메가-9 특성을 갖는 향상된 캐놀라박 영양가를 전달하는 종자 조성을 나타내는 캐놀라 생식질
US8648230B2 (en)	2011-03-18	2014-02-11	Ms Technologies, Llc	Regulatory regions preferentially expressing in non-pollen plant tissue
AU2012230804B2 (en)	2011-03-23	2016-12-22	Corteva Agriscience Llc	Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants
CN103534267B (zh)	2011-03-25	2017-07-14	孟山都技术公司	植物调控元件及其用途
US8513487B2 (en)	2011-04-07	2013-08-20	Zenon LISIECZKO	Plants and seeds of spring canola variety ND-662c
US8513494B2 (en)	2011-04-08	2013-08-20	Chunren Wu	Plants and seeds of spring canola variety SCV695971
GB2490655A (en)	2011-04-28	2012-11-14	Univ Aston	Modulators of tissue transglutaminase
US8507761B2 (en)	2011-05-05	2013-08-13	Teresa Huskowska	Plants and seeds of spring canola variety SCV372145
US8513495B2 (en)	2011-05-10	2013-08-20	Dale Burns	Plants and seeds of spring canola variety SCV291489
BR122019018085B1 (pt)	2011-05-13	2021-07-06	Monsanto Technology Llc	Molécula de dna recombinante e processo para expressão de uma molécula de polinucleotídeo que pode ser transcrita
GB201109239D0 (en)	2011-06-01	2011-07-13	Syngenta Participations Ag	Herbicidal compositions
GB201115280D0 (en)	2011-09-05	2011-10-19	Alligator Bioscience Ab	Antibodies, uses and methods
US10829828B2 (en)	2011-09-13	2020-11-10	Monsanto Technology Llc	Methods and compositions for weed control
CN107739737A (zh)	2011-09-13	2018-02-27	孟山都技术公司	用于杂草控制的方法和组合物
EP2756084B1 (en)	2011-09-13	2020-06-03	Monsanto Technology LLC	Methods and compositions for weed control
US9840715B1 (en)	2011-09-13	2017-12-12	Monsanto Technology Llc	Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
US10806146B2 (en)	2011-09-13	2020-10-20	Monsanto Technology Llc	Methods and compositions for weed control
AR087862A1 (es)	2011-09-13	2014-04-23	Monsanto Technology Llc	Metodos y composiciones para el control de malezas
US10760086B2 (en)	2011-09-13	2020-09-01	Monsanto Technology Llc	Methods and compositions for weed control
WO2013040116A1 (en)	2011-09-13	2013-03-21	Monsanto Technology Llc	Methods and compositions for weed control
UA115534C2 (uk)	2011-09-13	2017-11-27	Монсанто Текнолоджи Ллс	Спосіб та композиція для боротьби з бур'янами (варіанти)
CN110066794A (zh)	2011-09-13	2019-07-30	孟山都技术公司	用于杂草控制的方法和组合物
CN103930549B (zh)	2011-09-13	2020-09-18	孟山都技术公司	用于杂草控制的方法和组合物
EP3382027A3 (en)	2011-09-13	2018-10-31	Monsanto Technology LLC	Methods and compositions for weed control
US9920326B1 (en)	2011-09-14	2018-03-20	Monsanto Technology Llc	Methods and compositions for increasing invertase activity in plants
CN103998611A (zh)	2011-11-03	2014-08-20	先正达参股股份有限公司	用于增强植物中光同化作用的多核苷酸、多肽和方法
WO2013096818A1 (en)	2011-12-21	2013-06-27	The Curators Of The University Of Missouri	Soybean variety s05-11268
WO2013096810A1 (en)	2011-12-21	2013-06-27	The Curators Of The University Of Missouri	Soybean variety s05-11482
US20130180008A1 (en)	2012-01-06	2013-07-11	Pioneer Hi Bred International Inc	Ovule Specific Promoter and Methods of Use
WO2013103365A1 (en)	2012-01-06	2013-07-11	Pioneer Hi-Bred International, Inc.	Pollen preferred promoters and methods of use
ES2716481T3 (es)	2012-02-01	2019-06-12	Dow Agrosciences Llc	Plantas resistentes al glifosato y métodos asociados
AU2013219927B2 (en)	2012-02-16	2018-11-08	Syngenta Crop Protection Ag	Engineered pesticidal proteins
CN104411827B (zh)	2012-02-29	2018-01-02	陶氏益农公司	甘蔗杆状病毒(scbv)增强子及其在植物功能基因组学中的用途
US20150203864A1 (en)	2012-03-13	2015-07-23	University Of Guelph	Myb55 promoter and use thereof
US9663793B2 (en)	2012-04-20	2017-05-30	Monsanto Technology, Llc	Plant regulatory elements and uses thereof
US8835720B2 (en)	2012-04-26	2014-09-16	Monsanto Technology Llc	Plants and seeds of spring canola variety SCV967592
US8802935B2 (en)	2012-04-26	2014-08-12	Monsanto Technology Llc	Plants and seeds of spring canola variety SCV942568
US8878009B2 (en)	2012-04-26	2014-11-04	Monsanto Technology, LLP	Plants and seeds of spring canola variety SCV318181
US8859857B2 (en)	2012-04-26	2014-10-14	Monsanto Technology Llc	Plants and seeds of spring canola variety SCV259778
EP2855680B8 (en)	2012-05-24	2020-04-15	Monsanto Technology LLC	Compositions and methods for silencing gene expression
RU2619178C2 (ru)	2012-06-07	2017-05-12	ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи	Конструкт и способ для экспрессии трансгенов с использованием двунаправленного конститутивного промотора brassica
US20150225734A1 (en)	2012-06-19	2015-08-13	Regents Of The University Of Minnesota	Gene targeting in plants using dna viruses
US9752154B2 (en)	2012-07-26	2017-09-05	Dow Agrosciences Llc	High-throughput DNA fragment assembly
AU2013302947B2 (en)	2012-08-17	2017-06-01	Diaa ALABED	Use of a maize untranslated region for transgene expression in plants
US9914930B2 (en)	2012-09-07	2018-03-13	Dow Agrosciences Llc	FAD3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks
UA118090C2 (uk)	2012-09-07	2018-11-26	ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі	Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
CA2887571A1 (en)	2012-10-11	2014-04-17	Pioneer Hi-Bred International, Inc.	Guard cell promoters and uses thereof
MX364070B (es)	2012-10-18	2019-04-10	Monsanto Technology Llc	Métodos y composiciones para el control de plagas vegetales.
CN104968193B (zh)	2012-11-01	2021-02-09	塞尔克蒂斯股份有限公司	用于生成治疗性蛋白质的植物
GB201219678D0 (en)	2012-11-01	2012-12-12	Benf Ab	Ketone body inhibitors and uses thereof
US20150307894A1 (en)	2012-11-28	2015-10-29	Monsanto Technology Llc	Transgenic Plants With Enhanced Traits
PL2934097T3 (pl)	2012-12-21	2018-11-30	Cellectis	Ziemniaki o ograniczonej słodkości indukowanej chłodem
UY35251A (es)	2013-01-01	2014-07-31	Seeds Ltd Ab	MOLÉCULAS DE dsRNA AISLADAS Y MÉTODOS PARA USARLAS PARA SILENCIAR MOLÉCULAS DIANA DE INTERÉS
US10683505B2 (en)	2013-01-01	2020-06-16	Monsanto Technology Llc	Methods of introducing dsRNA to plant seeds for modulating gene expression
US20140203176A1 (en)	2013-01-23	2014-07-24	Dow Agrosciences Llc	Systems and methods for real-time sampling and analysis of biomolecules beneath the surface of biological tissue
US10000767B2 (en)	2013-01-28	2018-06-19	Monsanto Technology Llc	Methods and compositions for plant pest control
CN105026419B (zh)	2013-03-01	2021-08-24	勃林格殷格翰动物保健美国有限公司	疫苗组合物定量
UA121846C2 (uk)	2013-03-13	2020-08-10	Монсанто Текнолоджи Ллс	Спосіб та гербіцидна композиція для контролю видів рослини роду lolium
WO2014159306A1 (en)	2013-03-13	2014-10-02	Pioneer Hi-Bred International, Inc.	Glyphosate application for weed control in brassica
CN105074008A (zh)	2013-03-13	2015-11-18	孟山都技术有限公司	用于杂草控制的方法和组合物
MX369750B (es)	2013-03-14	2019-11-20	Pioneer Hi Bred Int	Composiciones y metodos para controlar plagas de insectos.
US20140283211A1 (en)	2013-03-14	2014-09-18	Monsanto Technology Llc	Methods and Compositions for Plant Pest Control
US10568328B2 (en)	2013-03-15	2020-02-25	Monsanto Technology Llc	Methods and compositions for weed control
US10023877B2 (en)	2013-03-15	2018-07-17	Pioneer Hi-Bred International, Inc.	PHI-4 polypeptides and methods for their use
US10113162B2 (en)	2013-03-15	2018-10-30	Cellectis	Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes
US9850496B2 (en)	2013-07-19	2017-12-26	Monsanto Technology Llc	Compositions and methods for controlling Leptinotarsa
EP3608412A3 (en)	2013-07-19	2020-04-08	Monsanto Technology LLC	Compositions and methods for controlling leptinotarsa
WO2015013509A1 (en)	2013-07-25	2015-01-29	Pioneer Hi-Bred International, Inc.	Method for producing hybrid brassica seed
TWI776192B (zh)	2013-08-05	2022-09-01	德商伊瑪提克斯生物科技有限公司	新穎肽類，細胞及其用於治療多種腫瘤的用途，其製造方法及包含其等之醫藥組成物（四）
MX359026B (es)	2013-08-16	2018-09-12	Pioneer Hi Bred Int	Proteinas insecticidas y metodos de uso.
US10667524B2 (en)	2013-09-13	2020-06-02	Pioneer Hi-Bred International, Inc.	Insecticidal proteins and methods for their use
US20160237447A1 (en)	2013-10-07	2016-08-18	Monsanto Technology Llc	Transgenic Plants With Enhanced Traits
GB201319446D0 (en)	2013-11-04	2013-12-18	Immatics Biotechnologies Gmbh	Personalized immunotherapy against several neuronal and brain tumors
BR102014027466B1 (pt)	2013-11-04	2022-09-27	Dow Agrosciences Llc	Molécula de ácido nucleico recombinante, método para produzir uma célula vegetal transgênica e usos de uma planta de soja, parte de planta de soja ou célula de planta de soja transgênica
JP6634022B2 (ja)	2013-11-04	2020-01-22	ダウアグロサイエンシィズエルエルシー	最適なダイズ遺伝子座
UA120503C2 (uk)	2013-11-04	2019-12-26	Дау Агросайєнсиз Елелсі	Спосіб одержання трансгенної клітини рослини кукурудзи
US9540642B2 (en)	2013-11-04	2017-01-10	The United States Of America, As Represented By The Secretary Of Agriculture	Compositions and methods for controlling arthropod parasite and pest infestations
CA2928666C (en)	2013-11-04	2023-05-23	Dow Agrosciences Llc	Optimal maize loci for targeted genome modification
WO2015085299A1 (en)	2013-12-06	2015-06-11	Altria Client Services Inc.	Tobacco plants having altered amounts of one or more alkaloids in leaf and methods of using such plants
UA119253C2 (uk)	2013-12-10	2019-05-27	Біолоджикс, Інк.	Спосіб боротьби із вірусом у кліща varroa та у бджіл
TW201527314A (zh)	2013-12-31	2015-07-16	Dow Agrosciences Llc	新穎玉米泛素啓動子（三）
US10683513B2 (en)	2013-12-31	2020-06-16	Dow Agrosciences Llc	Tissue-specific expression and hybrid plant production
TW201527312A (zh)	2013-12-31	2015-07-16	Dow Agrosciences Llc	新穎玉米泛素啓動子（一）
TW201527313A (zh)	2013-12-31	2015-07-16	Dow Agrosciences Llc	新穎玉米泛素啓動子（二）
TW201527316A (zh)	2013-12-31	2015-07-16	Dow Agrosciences Llc	新穎玉米泛素啓動子（五）
MX368629B (es)	2014-01-15	2019-10-08	Monsanto Technology Llc	Metodos y composiciones para el control de malezas utilizando polinucleotidos de 5-enolpiruvilshikimato-3-fosfato sintasa (epsps).
WO2015120276A1 (en)	2014-02-07	2015-08-13	Pioneer Hi Bred International Inc	Insecticidal proteins and methods for their use
CN114763376A (zh)	2014-02-07	2022-07-19	先锋国际良种公司	杀昆虫蛋白及其使用方法
TW201538518A (zh)	2014-02-28	2015-10-16	Dow Agrosciences Llc	藉由嵌合基因調控元件所賦予之根部特異性表現
WO2015153339A2 (en)	2014-04-01	2015-10-08	Monsanto Technology Llc	Compositions and methods for controlling insect pests
GB201408255D0 (en)	2014-05-09	2014-06-25	Immatics Biotechnologies Gmbh	Novel immunotherapy against several tumours of the blood, such as acute myeloid leukemia (AML)
EP3158072B1 (en)	2014-06-20	2021-01-13	Cellectis	Potatoes with reduced granule-bound starch synthase
GB201411037D0 (en)	2014-06-20	2014-08-06	Immatics Biotechnologies Gmbh	Novel immunotherapy against several tumors of the blood, in particular chronic lymphoid leukemai (CLL)
AU2015280252A1 (en)	2014-06-23	2017-01-12	Monsanto Technology Llc	Compositions and methods for regulating gene expression via RNA interference
EP3161138A4 (en)	2014-06-25	2017-12-06	Monsanto Technology LLC	Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
US10113174B2 (en)	2014-07-02	2018-10-30	Altria Client Services Llc	Tobacco having altered leaf properties and methods of making and using
WO2016007632A1 (en)	2014-07-08	2016-01-14	Altria Client Services Llc	The genetic locus imparting a low anatabine trait in tobacco and methods of using
AR101348A1 (es)	2014-07-29	2016-12-14	Monsanto Technology Llc	Composiciones y métodos para el control de pestes por insectos
WO2016022516A1 (en)	2014-08-08	2016-02-11	Pioneer Hi-Bred International, Inc.	Ubiquitin promoters and introns and methods of use
WO2016044092A1 (en)	2014-09-17	2016-03-24	Pioneer Hi Bred International Inc	Compositions and methods to control insect pests
US10370673B2 (en)	2014-09-26	2019-08-06	Purecircle Sdn Bhd	Single nucleotide polymorphism (SNP) markers for stevia
CN107250355B (zh)	2014-10-06	2021-07-16	奥驰亚客户服务有限公司	烟草植物中腋芽生长的遗传控制
EP3207143B1 (en)	2014-10-16	2023-11-22	Pioneer Hi-Bred International, Inc.	Insecticidal proteins and methods for their use
RU2741833C2 (ru)	2014-12-12	2021-01-29	Зингента Партисипейшнс Аг	Композиции и способы контроля вредителей растений
US20170359965A1 (en)	2014-12-19	2017-12-21	E I Du Pont De Nemours And Company	Polylactic acid compositions with accelerated degradation rate and increased heat stability
KR102643923B1 (ko)	2014-12-23	2024-03-05	이매틱스 바이오테크놀로지스 게엠베하	간세포 암종(hcc) 및 기타 암들에 대한 면역요법에서의 사용을 위한 신규 펩티드 및 펩티드의 조합
GB201501017D0 (en)	2014-12-23	2015-03-04	Immatics Biotechnologies Gmbh	Novel peptides and combination of peptides for use in immunotherapy against hepatocellular carcinoma (HCC) and other cancers
EP3244727B1 (en)	2015-01-15	2025-10-22	Pioneer Hi-Bred International, Inc.	Insecticidal proteins and methods for their use
JP6942632B2 (ja)	2015-01-22	2021-09-29	モンサントテクノロジーエルエルシー	Ｌｅｐｔｉｎｏｔａｒｓａ防除用組成物及びその方法
EP3274462A4 (en)	2015-03-26	2018-12-26	The Texas A&M University System	Conversion of lignin into bioplastics and lipid fuels
MD3388075T2 (ro)	2015-03-27	2023-10-31	Immatics Biotechnologies Gmbh	Noi peptide și combinații de peptide pentru utilizare în imunoterapia împotriva unor diverse tumori (SEQ ID 25 - MXRA5-003)
GB201505585D0 (en)	2015-03-31	2015-05-13	Immatics Biotechnologies Gmbh	Novel peptides and combination of peptides and scaffolds for use in immunotherapy against renal cell carinoma (RCC) and other cancers
GB201507030D0 (en)	2015-04-24	2015-06-10	Immatics Biotechnologies Gmbh	Immunotherapy against lung cancers, in particular NSCLC
GB201507719D0 (en)	2015-05-06	2015-06-17	Immatics Biotechnologies Gmbh	Novel peptides and combination of peptides and scaffolds thereof for use in immunotherapy against colorectal carcinoma (CRC) and other cancers
CN108064233B (zh)	2015-05-19	2022-07-15	先锋国际良种公司	杀昆虫蛋白及其使用方法
EP3302053B1 (en)	2015-06-02	2021-03-17	Monsanto Technology LLC	Compositions and methods for delivery of a polynucleotide into a plant
WO2016196782A1 (en)	2015-06-03	2016-12-08	Monsanto Technology Llc	Methods and compositions for introducing nucleic acids into plants
MX2017016119A (es)	2015-06-16	2018-03-07	Pioneer Hi Bred Int	Composiciones y metodos para controlar plagas de insectos.
WO2016205749A1 (en)	2015-06-18	2016-12-22	The Broad Institute Inc.	Novel crispr enzymes and systems
GB201511546D0 (en)	2015-07-01	2015-08-12	Immatics Biotechnologies Gmbh	Novel peptides and combination of peptides for use in immunotherapy against ovarian cancer and other cancers
PE20230321A1 (es)	2015-07-01	2023-02-22	Immatics Biotechnologies Gmbh	Nuevos peptidos y nuevas combinaciones de peptidos para el uso en la inmunoterapia contra el cancer de ovario y otros tipos de cancer
KR102890065B1 (ko)	2015-07-06	2025-11-21	이매틱스 바이오테크놀로지스 게엠베하	식도암 및 기타 암에 대한 면역요법에 사용하기 위한 펩티드 및 펩티드의 조합
GB201513921D0 (en)	2015-08-05	2015-09-23	Immatics Biotechnologies Gmbh	Novel peptides and combination of peptides for use in immunotherapy against prostate cancer and other cancers
EP3943602A1 (en)	2015-08-06	2022-01-26	Pioneer Hi-Bred International, Inc.	Plant derived insecticidal proteins and methods for their use
JP2018527931A (ja)	2015-08-28	2018-09-27	パイオニアハイ−ブレッドインターナショナル，インコーポレイテッド	植物のオクロバクテリウム（ｏｃｈｒｏｂａｃｔｒｕｍ）媒介形質転換
US10837024B2 (en)	2015-09-17	2020-11-17	Cellectis	Modifying messenger RNA stability in plant transformations
WO2017059341A1 (en)	2015-10-02	2017-04-06	Monsanto Technology Llc	Recombinant maize b chromosome sequence and uses thereof
WO2017083092A1 (en)	2015-11-10	2017-05-18	Dow Agrosciences Llc	Methods and systems for predicting the risk of transgene silencing
CN108575091A (zh)	2015-12-18	2018-09-25	先锋国际良种公司	杀昆虫蛋白及其使用方法
GB201522667D0 (en)	2015-12-22	2016-02-03	Immatics Biotechnologies Gmbh	Novel peptides and combination of peptides for use in immunotherapy against breast cancer and other cancers
US11104911B2 (en)	2015-12-22	2021-08-31	Pioneer Hi-Bred International, Inc.	Embryo-preferred Zea mays promoters and methods of use
WO2017132330A1 (en)	2016-01-26	2017-08-03	Monsanto Technology Llc	Compositions and methods for controlling insect pests
ES3039237T3 (en)	2016-02-02	2025-10-20	Cellectis	Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes
GB201602918D0 (en)	2016-02-19	2016-04-06	Immatics Biotechnologies Gmbh	Novel peptides and combination of peptides for use in immunotherapy against NHL and other cancers
IL302705A (en)	2016-03-01	2023-07-01	Immatics Biotechnologies Gmbh	Peptides, combinations of peptides, cell-based drugs for use in immunotherapy against bladder cancer and other types of cancer
GB201603987D0 (en)	2016-03-08	2016-04-20	Immatics Biotechnologies Gmbh	Uterine cancer treatments
IL261787B2 (en)	2016-03-16	2023-03-01	Immatics Biotechnologies Gmbh	Nucleic acid-treated t-cells and receptor t-cells for use in immunotherapy against types of cancer
CN116731156A (zh)	2016-03-16	2023-09-12	伊玛提克斯生物技术有限公司	用于癌症免疫治疗的转染t细胞和t细胞受体
CA3020162A1 (en)	2016-04-06	2017-10-12	Immatics Biotechnologies Gmbh	Novel peptides and combination of peptides for use in immunotherapy against aml and other cancers
WO2017192560A1 (en)	2016-05-04	2017-11-09	Pioneer Hi-Bred International, Inc.	Insecticidal proteins and methods for their use
CN114836567A (zh)	2016-06-16	2022-08-02	纽希得营养澳大利亚私人有限公司	优良种事件油菜ns-b50027-4
IL296433B2 (en)	2016-06-16	2024-06-01	Nuseed Nutritional Australia Pty Ltd	Inbred transgenic canola line ns-b50027-4 and seeds thereof
EP3472323A1 (en)	2016-06-16	2019-04-24	Pioneer Hi-Bred International, Inc.	Compositions and methods to control insect pests
CN109642237B (zh)	2016-06-24	2022-09-30	先锋国际良种公司	植物调节元件及其使用方法
WO2018005589A1 (en)	2016-06-28	2018-01-04	Cellectis	Altering expression of gene products in plants through targeted insertion of nucleic acid sequences
WO2018005491A1 (en)	2016-06-28	2018-01-04	Monsanto Technology Llc	Methods and compositions for use in genome modification in plants
US11155829B2 (en)	2016-07-01	2021-10-26	Pioneer Hi-Bred International, Inc.	Insecticidal proteins from plants and methods for their use
US20210292778A1 (en)	2016-07-12	2021-09-23	Pioneer Hi-Bred International, Inc.	Compositions and methods to control insect pests
TW202304970A (zh)	2016-08-26	2023-02-01	德商英麥提克生物技術股份有限公司	用於頭頸鱗狀細胞癌和其他癌症免疫治療的新型肽和支架
US12499971B2 (en)	2016-09-28	2025-12-16	The Broad Institute, Inc.	Systematic screening and mapping of regulatory elements in non-coding genomic regions, methods, compositions, and applications thereof
US11266174B2 (en)	2016-10-07	2022-03-08	Altria Client Services Llc	Tobacco plants having increased nitrogen efficiency and methods of using such plants
BR112019008800A2 (pt)	2016-11-01	2019-07-16	Pioneer Hi-Bred International, Inc.	polipeptídeo inseticida, composição inseticida, polinucleotídeo recombinante, construto de dna, célula de planta ou planta transgênica, método para inibir o crescimento ou exterminar uma população de praga de inseto agrícola, método para inibir o crescimento ou exterminar uma praga de inseto, método para controlar infestação de inseto lepidoptera e/ou coleoptera em uma planta transgênica e fornecer gerenciamento de resistência de inseto e uso de pelo menos um polipeptídeo inseticida
UY37482A (es)	2016-11-16	2018-05-31	Cellectis	Métodos para alterar el contenido de aminoácidos en plantas mediante mutaciones de desplazamiento de marco
WO2018138257A1 (en)	2017-01-27	2018-08-02	Immatics Biotechnologies Gmbh	Novel peptides and combination of peptides for use in immunotherapy against ovarian cancer and other cancers
US10899819B2 (en)	2017-04-10	2021-01-26	Immatics Biotechnologies Gmbh	Peptides and combination of peptides for use in immunotherapy against leukemias and other cancers
PE20191711A1 (es)	2017-04-10	2019-11-28	Immatics Biotechnologies Gmbh	Peptidos y combinaciones de los mismos para el uso en la inmunoterapia contra diversos tipos de cancer
US11427614B2 (en)	2017-04-10	2022-08-30	Immatics Biotechnologies Gmbh	Peptides and combination thereof for use in the immunotherapy against cancers
AU2018260469B2 (en)	2017-04-25	2024-09-05	Cellectis	Alfalfa with reduced lignin composition
US11591601B2 (en)	2017-05-05	2023-02-28	The Broad Institute, Inc.	Methods for identification and modification of lncRNA associated with target genotypes and phenotypes
WO2018213726A1 (en)	2017-05-18	2018-11-22	The Broad Institute, Inc.	Systems, methods, and compositions for targeted nucleic acid editing
WO2019007974A1 (en)	2017-07-07	2019-01-10	Immatics Biotechnologies Gmbh	NOVEL PEPTIDES AND COMBINATION OF PEPTIDES FOR USE IN IMMUNOTHERAPY OF LUNG CANCER, INCLUDING NSCLC, CPPC AND OTHER CANCERS
CR20210169A (es)	2017-07-07	2021-06-10	Immatics Biotechnologies Gmbh	NUEVOS PÉPTIDOS Y NUEVAS COMBINACIONES DE PÉPTIDOS PARA EL USO DE LA INMUNOTERAPIA CONTRA EL CÁNCER DE PULMÓN, INCLUYENDO EL NSCLC, EL SCLC Y OTROS CÁNCERES (Divisional 2020-0059)
RU2020111575A (ru)	2017-08-22	2021-09-23	Напиджен, Инк.	Модификация генома органелл с использованием направляемой полинуклеотидом эндонуклеазы
WO2019046756A1 (en)	2017-09-01	2019-03-07	Altria Client Services Llc	METHODS AND COMPOSITIONS ASSOCIATED WITH ENHANCED USE OF NITROGEN IN TOBACCO
WO2019060746A1 (en)	2017-09-21	2019-03-28	The Broad Institute, Inc.	SYSTEMS, METHODS, AND COMPOSITIONS FOR THE TARGETED EDITING OF NUCLEIC ACIDS
EP3688171A1 (en)	2017-09-25	2020-08-05	Pioneer Hi-Bred International, Inc.	Tissue-preferred promoters and methods of use
EP3691453A4 (en)	2017-10-02	2021-07-07	Syngenta Participations AG	MANIPULATED PESTICIDE PROTEINS AND METHODS OF CONTROLLING PLANT PESTURES
WO2019126709A1 (en)	2017-12-22	2019-06-27	The Broad Institute, Inc.	Cas12b systems, methods, and compositions for targeted dna base editing
EP3737747A1 (en)	2018-01-12	2020-11-18	The Texas A&M University System	Increasing plant bioproduct yield
DE102018107224A1 (de)	2018-02-21	2019-08-22	Immatics Biotechnologies Gmbh	Peptide und Kombinationen von Peptiden nicht-kanonischen Ursprungs zur Verwendung in der Immuntherapie gegen verschiedene Krebsarten
ES3036459T3 (en)	2018-03-12	2025-09-19	Pioneer Hi Bred Int	Methods for plant transformation
CA3092078A1 (en)	2018-03-14	2019-09-19	Pioneer Hi-Bred International, Inc.	Insecticidal proteins from plants and methods for their use
CN116410286A (zh)	2018-03-14	2023-07-11	先锋国际良种公司	来自植物的杀昆虫蛋白及其使用方法
US10968257B2 (en)	2018-04-03	2021-04-06	The Broad Institute, Inc.	Target recognition motifs and uses thereof
TW202016131A (zh)	2018-05-16	2020-05-01	德商英麥提克生物技術股份有限公司	用於抗癌免疫治療的肽
WO2019226508A1 (en)	2018-05-22	2019-11-28	Pioneer Hi-Bred International, Inc.	Plant regulatory elements and methods of use thereof
BR112020026640A2 (pt)	2018-06-28	2021-04-06	Pioneer Hi-Bred International, Inc.	Métodos para selecionar plantas transformadas
US10925947B2 (en)	2018-06-29	2021-02-23	Immatics Biotechnologies Gmbh	A*03 restricted peptides for use in immunotherapy against cancers and related methods
TW202019955A (zh)	2018-07-31	2020-06-01	德商英麥提克生物技術股份有限公司	B*07 限制肽和肽組合的抗癌免疫治療和相關方法
TW202028224A (zh)	2018-09-17	2020-08-01	德商英麥提克生物技術股份有限公司	B*44限制肽在抗癌免疫治療的用途和相關方法
TW202024121A (zh)	2018-09-18	2020-07-01	德商英麥提克生物技術股份有限公司	A*01 限制肽和肽組合物在抗癌免疫治療中的用途和相關方法
AU2019369415A1 (en)	2018-10-31	2021-03-25	Pioneer Hi-Bred International, Inc.	Compositions and methods for Ochrobactrum-mediated plant transformation
CA3124110A1 (en)	2018-12-17	2020-06-25	The Broad Institute, Inc.	Crispr-associated transposase systems and methods of use thereof
TW202039535A (zh)	2018-12-18	2020-11-01	德商英麥提克生物技術股份有限公司	B*08限制肽和肽組合物抗癌免疫治療和相關方法
PH12021551717A1 (en)	2019-02-20	2022-03-28	Syngenta Crop Protection Ag	Engineered pesticidal proteins and methods of controlling plant pests
CN115087347B (zh)	2019-11-01	2024-05-17	谱赛科美国股份有限公司	甜菊栽培品种‘18136109’
CN115867564A (zh)	2020-07-14	2023-03-28	先锋国际良种公司	杀昆虫蛋白及其使用方法
EP4203677A1 (en)	2020-08-26	2023-07-05	Vindara, Inc.	Method and/or compositions for lettuce (lactuca sativa) breeding and/or varieties developed thereby
DE102020125457A1 (de)	2020-09-29	2022-03-31	Immatics Biotechnologies Gmbh	Amidierte Peptide und ihre deamidierten Gegenstücke, die durch HLA-A*02-Moleküle präsentiert werden, zur Verwendung in der Immuntherapie gegen verschiedene Krebsarten
TW202229312A (zh)	2020-09-29	2022-08-01	德商英麥提克生物技術股份有限公司	由非ｈｌａ－ａ*０２顯露以用於不同類型癌症之免疫治療的醯胺化肽及其脫醯胺化對應物
TW202241925A (zh)	2021-01-15	2022-11-01	德商英麥提克生物技術股份有限公司	用於不同類型癌症免疫治療的ｈｌａ展示肽
WO2023086765A2 (en)	2021-11-12	2023-05-19	Monsanto Technology Llc	Compositions and methods for altering plant determinacy
CN118843637A (zh)	2022-02-11	2024-10-25	东北农业大学	用于增加植物中蛋白质和/或油含量和改变油特性的方法和组合物
WO2024020360A1 (en)	2022-07-18	2024-01-25	Pairwise Plants Services, Inc.	Mustard green plants named 'pwrg-1', 'pwrg-2,' and 'pwsgc'
EP4658770A1 (en)	2023-02-03	2025-12-10	Syngenta Crop Protection AG	Herbicide resistant plants
PY2430924A (es)	2023-04-19	2025-02-17	Syngenta Crop Protection Ag	Plantas resistentes a herbicidas
WO2025040598A2 (en)	2023-08-18	2025-02-27	Immatics Biotechnologies Gmbh	Peptides displayed by mhc for use in immunotherapy against different types of cancer
WO2026006779A1 (en)	2024-06-27	2026-01-02	The Regents Of The University Of California	Transgenic plants expressing fern rapid non-photochemical quenching (npq) proteins
WO2026027723A1 (en)	2024-08-01	2026-02-05	Syngenta Crop Protection Ag	Herbicide resistant plants

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4438032A (en) *	1981-01-30	1984-03-20	The Regents Of The University Of California	Unique T-lymphocyte line and products derived therefrom
JPS61502166A (ja) *	1984-04-19	1986-10-02	アグラシタス	植物細胞の形質転換のための改良された方法及びベクタ−
WO1986003516A1 (en) *	1984-12-13	1986-06-19	Biotechnica International, Inc.	Plant tranformation vector
KR950008571B1 (ko) *	1986-01-08	1995-08-03	롱쁠랑 아그로시미	할로아릴니트릴 분해 유전자, 그의 용도 및 이를 함유한 세포

1987
- 1987-07-08 US US07/071,146 patent/US4810648A/en not_active Expired - Lifetime
1988
- 1988-06-28 GR GR880100421A patent/GR880100421A/el not_active IP Right Cessation
- 1988-07-04 KR KR1019890700419A patent/KR890701735A/ko not_active Withdrawn
- 1988-07-04 AU AU20719/88A patent/AU2071988A/en not_active Abandoned
- 1988-07-04 WO PCT/EP1988/000588 patent/WO1989000193A1/en not_active Ceased
- 1988-07-04 EP EP88905798A patent/EP0373173A1/en not_active Withdrawn
- 1988-07-04 BR BR888807609A patent/BR8807609A/pt unknown
- 1988-07-06 IL IL8700188A patent/IL87001A/en not_active IP Right Cessation
- 1988-07-07 CA CA000571422A patent/CA1339683C/en not_active Expired - Fee Related
- 1988-07-07 PL PL27358988A patent/PL273589A1/xx unknown
- 1988-07-07 ZA ZA884894A patent/ZA884894B/xx unknown
- 1988-07-07 ES ES8802134A patent/ES2010763A6/es not_active Expired
- 1988-07-08 DD DD88330727A patent/DD284048A5/de not_active IP Right Cessation
- 1988-07-08 DD DD88317745A patent/DD285922A5/de not_active IP Right Cessation
- 1988-07-08 CN CN88104173A patent/CN1031252A/zh active Pending
1990
- 1990-01-05 DK DK002890A patent/DK2890A/da not_active Application Discontinuation

Also Published As

Publication number	Publication date
IL87001A0 (en)	1988-12-30
ES2010763A6 (es)	1989-12-01
US4810648A (en)	1989-03-07
WO1989000193A1 (en)	1989-01-12
ZA884894B (en)	1990-04-25
IL87001A (en)	1994-08-26
DD285922A5 (de)	1991-01-10
DK2890A (da)	1990-03-02
GR880100421A (el)	1989-04-12
DK2890D0 (da)	1990-01-05
CN1031252A (zh)	1989-02-22
CA1339683C (en)	1998-02-24
KR890701735A (ko)	1989-12-21
BR8807609A (pt)	1990-05-29
PL273589A1 (en)	1989-04-03
EP0373173A1 (en)	1990-06-20
AU2071988A (en)	1989-01-30

Legal Events

Date	Code	Title	Description
1995-02-16	ENJ	Ceased due to non-payment of renewal fee
1995-03-02	ENJ	Ceased due to non-payment of renewal fee

Publication	Publication Date	Title
DD284048A5 (de)	1990-10-31	Verfahren zur herstellung einer pflanzenzelle bzw. eines pflanzenteils oder einer pflanze mit einer derartigen pflanzenzelle
DE69033957T2 (de)	2002-11-28	Herstellung von hybridem saatgut
DE3889546T2 (de)	1994-09-08	Transformation von keimenden pflanzensamen mit hilfe von agrobacterium.
JP2580499B2 (ja)	1997-02-12	ハロアリ―ルニトリル特異性細菌ニトリラ―ゼおよびその製法
DE69034081T2 (de)	2004-02-12	Krankheitsresistente transgene Pflanze
DE68923728T2 (de)	1996-02-22	Insektenresistente Salatpflanzen.
DE69028479T2 (de)	1997-04-03	Genetische Manipulationen mit von RNA-Viren abgeleiteten Sequenzen enthaltendem rekombinantem DNA
DE69333801T2 (de)	2006-01-19	Verfahren zur Herstellung von hybridem Saatgut
DE69318558T2 (de)	1998-09-10	Brassica regulatorische sequenz für wurzelspezifische oder wurzelreichliche gen expression
DE69024979T2 (de)	1996-10-17	Expression von unkrautvertilgungsmittel p450 metabolisierenden cytochromen
DE69636392T2 (de)	2006-11-23	Polypeptide und polynukleotide bezüglich alpha-und-beta-glutamat-dehydrogenase-untereinheiten und deren verwendung
DE69636225T2 (de)	2006-10-12	Stimulierung der homologen rekombination in pflanzlichen organismen mittels rekombinations fördernder enzyme
DE4004800C2 (de)	2000-12-21	Im Habitus und Ertrag veränderte transgene Pflanzen
EP0375091A1 (de)	1990-06-27	Wundinduzierbare und kartoffelknollenspezifische transkriptionale Regulation
EP0298918B1 (de)	2001-09-05	Induzierbare Virusresistenz bei Pflanzen
DE69333079T2 (de)	2004-05-13	Expressionskassette und plasmid für eine expression spezifisch für schliesszellen und ihre verwendung zur einführung in transgene pflanzenzellen und pflanzen
US6372888B1 (en)	2002-04-16	Antifungal proteins
EP0256223A1 (de)	1988-02-24	Herbizid-tolerante Pflanzen, enthaltend ein Glutathion-S-Transferase-Gen
DE68922669T2 (de)	1995-10-05	DNS-Konstruktionen und enthaltende Pflanzen.
EP0462065B1 (de)	2005-03-02	Neue Signalsequenzen
EP0418695A1 (de)	1991-03-27	Regulatorische DNA-Sequenz
DE69132935T2 (de)	2002-10-17	Histidinol Dehydrogenase Protein, DNS und Muteine und diese enthaltende transgenische Pflanzen
DE69633568T2 (de)	2005-12-22	Expressionskontrollesequenz zur allgemeinen und effektiven genexpression in pflanzen
DE69930629T2 (de)	2007-02-08	Induzierbare promotoren
DE69332148T2 (de)	2003-03-27	Verwendung einer dna sequenz, kodierende für ein protein mit der fäligkeit oxalsäure abzubauen, zur genselektion