BRPI0414959A - variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d - Google Patents
variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3dInfo
- Publication number
- BRPI0414959A BRPI0414959A BRPI0414959-9A BRPI0414959A BRPI0414959A BR PI0414959 A BRPI0414959 A BR PI0414959A BR PI0414959 A BRPI0414959 A BR PI0414959A BR PI0414959 A BRPI0414959 A BR PI0414959A
- Authority
- BR
- Brazil
- Prior art keywords
- protease variant
- protease
- nucleic acid
- composition
- animal feed
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/111—Antisense spanning the whole gene, or a large part of it
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Fodder In General (AREA)
- Detergent Compositions (AREA)
- Materials For Medical Uses (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
Abstract
"VARIANTE DE UMA PROTEASE PRECURSORA, SEQüêNCIA DE áCIDO NUCLEICO ISOLADO, CONSTRUçãO DE áCIDO NUCLEICO, VETOR DE EXPRESSãO E CéLULA HOSPEDEIRA RECOMBINANTES, MéTODOS PARA PRODUZIR A VARIANTE DE PROTEASE, PARA MELHORAR O VALOR NUTRICIONAL DE UMA RAçãO ANIMAL, E PARA TRATAR PROTEìNAS, PLANTA TRANSGêNICA, OU PARTE DA PLANTA, ANIMAL TRANSGêNICO, NãO HUMANO, OU PRODUTOS, OU ELEMENTOS DESTE, ADITIVO E COMPOSIçãO DE RAçãO ANIMAL, USO DA VARIANTE DE PROTEASE E/OU DA COMPOSIçãO, PROTEASE, E, ESTRUTURA 3D". A invenção diz respeito a uma nova estrutura 3D que codifica uma protease de Nocardiopsis, assim como às variantes da protease precursora homóloga às proteases de Nocardiopsis, preferivelmente de termoestabilidade melhorada e/ou com um perfil de atividade de temperatura aperfeiçoado. A invenção também diz respeito às seqüências de DNA que codificam tais variantes, sua produção em uma célula hospedeira recombinante, assim como os métodos de uso das variantes, em particular dentro do campo de ração animal e detergentes. A invenção além disso diz respeito aos métodos de gerar e preparar as variantes de protease de propriedades aperfeiçoadas.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200301494 | 2003-10-10 | ||
| DKPA200400333 | 2004-03-01 | ||
| PCT/DK2004/000688 WO2005035747A1 (en) | 2003-10-10 | 2004-10-08 | Protease variants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0414959A true BRPI0414959A (pt) | 2006-11-07 |
Family
ID=34436496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0414959-9A BRPI0414959A (pt) | 2003-10-10 | 2004-10-08 | variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d |
Country Status (11)
| Country | Link |
|---|---|
| US (4) | US7892808B2 (pt) |
| EP (3) | EP2308966A1 (pt) |
| JP (2) | JP2007509603A (pt) |
| CN (1) | CN1867668A (pt) |
| AT (1) | ATE491788T1 (pt) |
| AU (1) | AU2004279924B2 (pt) |
| BR (1) | BRPI0414959A (pt) |
| CA (1) | CA2539693A1 (pt) |
| DE (1) | DE602004030595D1 (pt) |
| ES (1) | ES2358092T3 (pt) |
| WO (1) | WO2005035747A1 (pt) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1594963B1 (en) * | 2003-02-07 | 2007-07-25 | Novozymes A/S | Proteases |
| EP1639107B1 (en) * | 2003-06-19 | 2013-08-14 | Novozymes A/S | Improved proteases and methods for producing them |
| BRPI0414959A (pt) | 2003-10-10 | 2006-11-07 | Novozymes As | variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d |
| DK1755656T3 (da) | 2004-05-24 | 2010-10-04 | Novozymes As | Enzymer til farmaceutisk anvendelse |
| CN101014704A (zh) | 2004-06-21 | 2007-08-08 | 诺维信公司 | 蛋白酶 |
| CN101489412B (zh) | 2006-07-13 | 2013-03-27 | 诺维信公司 | 细菌淀粉酶在牛类动物的饲料中的用途 |
| BRPI0720527B1 (pt) * | 2006-12-22 | 2017-06-13 | Novozymes A/S | Method for producing a yeast extract |
| CA2707658A1 (en) * | 2007-12-04 | 2009-06-11 | Novozymes A/S | Protease variants for pharmaceutical use |
| WO2009140481A1 (en) | 2008-05-14 | 2009-11-19 | Novozymes A/S | Liquid detergent compositions |
| US20110257087A1 (en) | 2008-12-31 | 2011-10-20 | Solae, Llc | Protein Hydrolysate Compositions Having Enhanced CCK Releasing Activity |
| CN102917600B (zh) * | 2010-03-26 | 2015-07-08 | 诺维信公司 | 热稳定性肌醇六磷酸酶变体 |
| CN103328654B (zh) | 2010-10-27 | 2017-07-11 | 哈佛学院院长等 | 立足点引物双链体的组合物及其使用方法 |
| CA2825119A1 (en) | 2011-02-23 | 2012-10-18 | Solae, Llc | Protein hydrolysate compositions having enhanced cck and glp-1 releasing activity |
| US20140295027A1 (en) | 2011-08-19 | 2014-10-02 | Novozymes A/S | Polypeptides Having Protease Activity |
| ES2628190T3 (es) | 2011-09-22 | 2017-08-02 | Novozymes A/S | Polipéptidos con actividad de proteasa y polinucleótidos que codifican los mismos |
| ES2667318T3 (es) | 2011-12-28 | 2018-05-10 | Novozymes A/S | Polipéptidos con actividad de proteasa |
| US9801398B2 (en) | 2012-01-26 | 2017-10-31 | Novozymes A/S | Use of polypeptides having protease activity in animal feed and detergents |
| MX364390B (es) * | 2012-06-20 | 2019-04-25 | Novozymes As | Uso de polipeptidos que tienen actividad proteasa en alimentos para animales y detergentes. |
| AU2013311668B2 (en) | 2012-09-05 | 2019-02-28 | Novozymes A/S | Polypeptides having protease activity |
| US9872937B2 (en) | 2012-09-19 | 2018-01-23 | Microvascular Tissues, Inc. | Compositions and methods for treating and preventing tissue injury and disease |
| US9551042B2 (en) | 2012-12-21 | 2017-01-24 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
| MX2015010078A (es) | 2013-02-06 | 2016-01-25 | Novozymes As | Uso de polipeptidos con actividad proteasa en alimentos para animales. |
| US10619145B2 (en) | 2014-11-04 | 2020-04-14 | Novozymes A/S | Polypeptides having serine protease activity and polynucleotides encoding same and their application in animal feed |
| ES2891085T3 (es) * | 2015-01-30 | 2022-01-26 | Harvard College | Obtención de imágenes sin microscopio |
| DE102016204815A1 (de) * | 2016-03-23 | 2017-09-28 | Henkel Ag & Co. Kgaa | Proteasen mit verbesserte Enzymstabilität in Waschmittel |
| DE102016204814A1 (de) * | 2016-03-23 | 2017-09-28 | Henkel Ag & Co. Kgaa | Verbesserte Reinigungsleistung an Protein sensitiven Anschmutzungen |
| US11492661B2 (en) | 2017-01-10 | 2022-11-08 | President And Fellows Of Harvard College | Multiplexed signal amplification |
| MX386582B (es) | 2017-03-15 | 2025-03-19 | Int N&H Denmark Aps | Métodos para usar una serina proteasa de arqueas. |
| WO2019147945A1 (en) | 2018-01-26 | 2019-08-01 | President And Fellows Of Harvard College | Proximity detection methods and compositions |
| US12142104B2 (en) | 2018-03-22 | 2024-11-12 | President And Fellows Of Harvard College | Methods and compositions for molecular authentication |
| CN110727780A (zh) * | 2019-10-17 | 2020-01-24 | 福建天晴数码有限公司 | 一种相识文本自动扩展的系统及其方法 |
| WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
| CN115074345B (zh) * | 2021-03-10 | 2024-04-05 | 中国农业科学院农业资源与农业区划研究所 | 耐热β-1,4-内切木聚糖酶及其编码基因与应用 |
| CN115772531B (zh) * | 2022-11-11 | 2024-11-12 | 吉林农业大学 | 一种果胶酸裂解酶基因突变体及其克隆方法和应用 |
| CN116103264B (zh) * | 2022-12-28 | 2025-05-09 | 厦门蓝湾科技有限公司 | 一种提高磷脂酶d合成ps活性的理性定向进化方法 |
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| BRPI0414959A (pt) * | 2003-10-10 | 2006-11-07 | Novozymes As | variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d |
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-
2004
- 2004-10-08 BR BRPI0414959-9A patent/BRPI0414959A/pt not_active Application Discontinuation
- 2004-10-08 EP EP10182980A patent/EP2308966A1/en not_active Ceased
- 2004-10-08 EP EP10182999A patent/EP2284259A3/en not_active Withdrawn
- 2004-10-08 CA CA002539693A patent/CA2539693A1/en not_active Abandoned
- 2004-10-08 ES ES04762907T patent/ES2358092T3/es not_active Expired - Lifetime
- 2004-10-08 WO PCT/DK2004/000688 patent/WO2005035747A1/en not_active Ceased
- 2004-10-08 AT AT04762907T patent/ATE491788T1/de not_active IP Right Cessation
- 2004-10-08 EP EP04762907A patent/EP1673451B1/en not_active Expired - Lifetime
- 2004-10-08 AU AU2004279924A patent/AU2004279924B2/en not_active Ceased
- 2004-10-08 DE DE602004030595T patent/DE602004030595D1/de not_active Expired - Lifetime
- 2004-10-08 US US10/574,554 patent/US7892808B2/en active Active
- 2004-10-08 CN CNA2004800297722A patent/CN1867668A/zh active Pending
- 2004-10-08 JP JP2006529655A patent/JP2007509603A/ja active Pending
-
2011
- 2011-01-05 US US12/984,826 patent/US8377677B2/en not_active Expired - Lifetime
-
2012
- 2012-01-27 JP JP2012014730A patent/JP2012095666A/ja active Pending
-
2013
- 2013-01-10 US US13/738,490 patent/US8772011B2/en not_active Expired - Fee Related
-
2014
- 2014-05-23 US US14/286,089 patent/US9131711B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| ES2358092T3 (es) | 2011-05-05 |
| US9131711B2 (en) | 2015-09-15 |
| US20090047387A1 (en) | 2009-02-19 |
| US20110097445A1 (en) | 2011-04-28 |
| EP2308966A1 (en) | 2011-04-13 |
| US7892808B2 (en) | 2011-02-22 |
| EP1673451B1 (en) | 2010-12-15 |
| CA2539693A1 (en) | 2005-04-21 |
| JP2012095666A (ja) | 2012-05-24 |
| WO2005035747A1 (en) | 2005-04-21 |
| JP2007509603A (ja) | 2007-04-19 |
| US8377677B2 (en) | 2013-02-19 |
| AU2004279924B2 (en) | 2010-09-30 |
| ATE491788T1 (de) | 2011-01-15 |
| CN1867668A (zh) | 2006-11-22 |
| US20130189732A1 (en) | 2013-07-25 |
| AU2004279924A1 (en) | 2005-04-21 |
| EP2284259A2 (en) | 2011-02-16 |
| US20140255550A1 (en) | 2014-09-11 |
| US8772011B2 (en) | 2014-07-08 |
| EP1673451A1 (en) | 2006-06-28 |
| DE602004030595D1 (de) | 2011-01-27 |
| EP2284259A3 (en) | 2011-06-15 |
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