BRPI0414959A - variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d - Google Patents

variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d

Info

Publication number
BRPI0414959A
BRPI0414959A BRPI0414959-9A BRPI0414959A BRPI0414959A BR PI0414959 A BRPI0414959 A BR PI0414959A BR PI0414959 A BRPI0414959 A BR PI0414959A BR PI0414959 A BRPI0414959 A BR PI0414959A
Authority
BR
Brazil
Prior art keywords
protease variant
protease
nucleic acid
composition
animal feed
Prior art date
Application number
BRPI0414959-9A
Other languages
English (en)
Inventor
Leonardo De Maria
Carsten Andersen
Lars Lehmann Hylli Christensen
Soren Flensted Lassen
Peter Ranbek Ostergaard
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of BRPI0414959A publication Critical patent/BRPI0414959A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Detergent Compositions (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Fodder In General (AREA)
  • Peptides Or Proteins (AREA)
  • Materials For Medical Uses (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)

Abstract

"VARIANTE DE UMA PROTEASE PRECURSORA, SEQüêNCIA DE áCIDO NUCLEICO ISOLADO, CONSTRUçãO DE áCIDO NUCLEICO, VETOR DE EXPRESSãO E CéLULA HOSPEDEIRA RECOMBINANTES, MéTODOS PARA PRODUZIR A VARIANTE DE PROTEASE, PARA MELHORAR O VALOR NUTRICIONAL DE UMA RAçãO ANIMAL, E PARA TRATAR PROTEìNAS, PLANTA TRANSGêNICA, OU PARTE DA PLANTA, ANIMAL TRANSGêNICO, NãO HUMANO, OU PRODUTOS, OU ELEMENTOS DESTE, ADITIVO E COMPOSIçãO DE RAçãO ANIMAL, USO DA VARIANTE DE PROTEASE E/OU DA COMPOSIçãO, PROTEASE, E, ESTRUTURA 3D". A invenção diz respeito a uma nova estrutura 3D que codifica uma protease de Nocardiopsis, assim como às variantes da protease precursora homóloga às proteases de Nocardiopsis, preferivelmente de termoestabilidade melhorada e/ou com um perfil de atividade de temperatura aperfeiçoado. A invenção também diz respeito às seqüências de DNA que codificam tais variantes, sua produção em uma célula hospedeira recombinante, assim como os métodos de uso das variantes, em particular dentro do campo de ração animal e detergentes. A invenção além disso diz respeito aos métodos de gerar e preparar as variantes de protease de propriedades aperfeiçoadas.
BRPI0414959-9A 2003-10-10 2004-10-08 variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d BRPI0414959A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200301494 2003-10-10
DKPA200400333 2004-03-01
PCT/DK2004/000688 WO2005035747A1 (en) 2003-10-10 2004-10-08 Protease variants

Publications (1)

Publication Number Publication Date
BRPI0414959A true BRPI0414959A (pt) 2006-11-07

Family

ID=34436496

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0414959-9A BRPI0414959A (pt) 2003-10-10 2004-10-08 variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d

Country Status (11)

Country Link
US (4) US7892808B2 (pt)
EP (3) EP2284259A3 (pt)
JP (2) JP2007509603A (pt)
CN (1) CN1867668A (pt)
AT (1) ATE491788T1 (pt)
AU (1) AU2004279924B2 (pt)
BR (1) BRPI0414959A (pt)
CA (1) CA2539693A1 (pt)
DE (1) DE602004030595D1 (pt)
ES (1) ES2358092T3 (pt)
WO (1) WO2005035747A1 (pt)

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AU2004279924A1 (en) 2005-04-21
US20130189732A1 (en) 2013-07-25
US8772011B2 (en) 2014-07-08
US20140255550A1 (en) 2014-09-11
US20110097445A1 (en) 2011-04-28
CA2539693A1 (en) 2005-04-21
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US7892808B2 (en) 2011-02-22
US8377677B2 (en) 2013-02-19
EP2308966A1 (en) 2011-04-13
US9131711B2 (en) 2015-09-15
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JP2012095666A (ja) 2012-05-24
US20090047387A1 (en) 2009-02-19
EP2284259A3 (en) 2011-06-15
DE602004030595D1 (de) 2011-01-27
EP1673451B1 (en) 2010-12-15
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ATE491788T1 (de) 2011-01-15
EP2284259A2 (en) 2011-02-16

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