BRPI0414959A - variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d - Google Patents
variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3dInfo
- Publication number
- BRPI0414959A BRPI0414959A BRPI0414959-9A BRPI0414959A BRPI0414959A BR PI0414959 A BRPI0414959 A BR PI0414959A BR PI0414959 A BRPI0414959 A BR PI0414959A BR PI0414959 A BRPI0414959 A BR PI0414959A
- Authority
- BR
- Brazil
- Prior art keywords
- protease variant
- protease
- nucleic acid
- composition
- animal feed
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/11—Antisense
- C12N2310/111—Antisense spanning the whole gene, or a large part of it
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Detergent Compositions (AREA)
- Peptides Or Proteins (AREA)
- Fodder In General (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Materials For Medical Uses (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
Abstract
"VARIANTE DE UMA PROTEASE PRECURSORA, SEQüêNCIA DE áCIDO NUCLEICO ISOLADO, CONSTRUçãO DE áCIDO NUCLEICO, VETOR DE EXPRESSãO E CéLULA HOSPEDEIRA RECOMBINANTES, MéTODOS PARA PRODUZIR A VARIANTE DE PROTEASE, PARA MELHORAR O VALOR NUTRICIONAL DE UMA RAçãO ANIMAL, E PARA TRATAR PROTEìNAS, PLANTA TRANSGêNICA, OU PARTE DA PLANTA, ANIMAL TRANSGêNICO, NãO HUMANO, OU PRODUTOS, OU ELEMENTOS DESTE, ADITIVO E COMPOSIçãO DE RAçãO ANIMAL, USO DA VARIANTE DE PROTEASE E/OU DA COMPOSIçãO, PROTEASE, E, ESTRUTURA 3D". A invenção diz respeito a uma nova estrutura 3D que codifica uma protease de Nocardiopsis, assim como às variantes da protease precursora homóloga às proteases de Nocardiopsis, preferivelmente de termoestabilidade melhorada e/ou com um perfil de atividade de temperatura aperfeiçoado. A invenção também diz respeito às seqüências de DNA que codificam tais variantes, sua produção em uma célula hospedeira recombinante, assim como os métodos de uso das variantes, em particular dentro do campo de ração animal e detergentes. A invenção além disso diz respeito aos métodos de gerar e preparar as variantes de protease de propriedades aperfeiçoadas.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DKPA200301494 | 2003-10-10 | ||
| DKPA200400333 | 2004-03-01 | ||
| PCT/DK2004/000688 WO2005035747A1 (en) | 2003-10-10 | 2004-10-08 | Protease variants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0414959A true BRPI0414959A (pt) | 2006-11-07 |
Family
ID=34436496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0414959-9A BRPI0414959A (pt) | 2003-10-10 | 2004-10-08 | variante de uma protease precursora, sequência de ácido nucleico isolado, construção de ácido nucleico, vetor de expressão e célula hospedeira recombinantes, métodos para produzir a variante de protease, para melhorar o valor nutricional de uma ração animal, e para tratar proteìnas, planta transgênica, ou parte da planta, animal transgênico, não humano, ou produtos, ou elementos deste, aditivo e composição de ração animal, uso da variante de protease e/ou da composição, protease, e, estrutura 3d |
Country Status (11)
| Country | Link |
|---|---|
| US (4) | US7892808B2 (pt) |
| EP (3) | EP2308966A1 (pt) |
| JP (2) | JP2007509603A (pt) |
| CN (1) | CN1867668A (pt) |
| AT (1) | ATE491788T1 (pt) |
| AU (1) | AU2004279924B2 (pt) |
| BR (1) | BRPI0414959A (pt) |
| CA (1) | CA2539693A1 (pt) |
| DE (1) | DE602004030595D1 (pt) |
| ES (1) | ES2358092T3 (pt) |
| WO (1) | WO2005035747A1 (pt) |
Families Citing this family (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1774504B (zh) * | 2003-02-07 | 2013-04-17 | 诺维信公司 | 蛋白酶 |
| US20060236414A1 (en) * | 2003-06-19 | 2006-10-19 | Novozymes A/S | Proteases and methods for producing them |
| ES2358092T3 (es) * | 2003-10-10 | 2011-05-05 | Novozymes A/S | Variantes de proteasa. |
| BRPI0510817A (pt) | 2004-05-24 | 2007-11-20 | Novozymes As | protease, uso de uma protease, composição farmacêutica, e, método para o tratamento de uma doença |
| ES2380105T3 (es) * | 2004-06-21 | 2012-05-08 | Novozymes A/S | Proteasas de Nocardiopsis |
| US9668501B2 (en) | 2006-07-13 | 2017-06-06 | Dsm Ip Assets B.V. | Use of bacterial amylases in feed for bovine animals |
| CN101558148B (zh) * | 2006-12-22 | 2014-05-21 | 诺维信公司 | 用于产生酵母提取物的方法 |
| WO2009071550A1 (en) * | 2007-12-04 | 2009-06-11 | Novozymes A/S | Protease variants for pharmaceutical use |
| US8691743B2 (en) | 2008-05-14 | 2014-04-08 | Novozymes A/S | Liquid detergent compositions |
| EP2384125B1 (en) | 2008-12-31 | 2013-07-17 | Solae, Llc | Protein hydrolysate compositions having enhanced cck releasing ability |
| EP2552232B1 (en) * | 2010-03-26 | 2016-07-06 | Novozymes A/S | Thermostable phytase variants |
| JP5960709B2 (ja) | 2010-10-27 | 2016-08-02 | プレジデント アンド フェローズ オブ ハーバード カレッジ | トーホールドプライマーデュプレックスの組成物およびその使用方法 |
| BR112013021468A2 (pt) | 2011-02-23 | 2017-07-04 | Solae Llc | composição de proteína hidrolisada, produto alimentício e método de indução da saciedade |
| CN103764822B (zh) | 2011-08-19 | 2016-11-23 | 诺维信公司 | 具有蛋白酶活性的多肽 |
| JP2014530598A (ja) | 2011-09-22 | 2014-11-20 | ノボザイムスアクティーゼルスカブ | プロテアーゼ活性を有するポリペプチドおよびこれをコードするポリヌクレオチド |
| MX358963B (es) | 2011-12-28 | 2018-09-11 | Novozymes As | Polipeptidos con actividad proteasa. |
| BR112014017919A2 (pt) | 2012-01-26 | 2017-06-27 | Novozymes As | uso de um poliptídeo variante, composição, polinucleotídeo isolado, constructo de ácido nucleico ou vetor de expressão, célula hospedeira de expressão recombinante, métodos de produção do pilipeptídeo, para melhoria do valor nutricional de uma ração animal, e para o tratamento de proteínas, planta, parte de planta ou célula de planta transgênica, aditivo de ração animal, ração animal, e, composição de ração animal ou detergente |
| AU2013279440B2 (en) * | 2012-06-20 | 2016-10-06 | Novozymes A/S | Use of polypeptides having protease activity in animal feed and detergents |
| CN110229802A (zh) | 2012-09-05 | 2019-09-13 | 诺维信公司 | 具有蛋白酶活性的多肽 |
| US9872937B2 (en) | 2012-09-19 | 2018-01-23 | Microvascular Tissues, Inc. | Compositions and methods for treating and preventing tissue injury and disease |
| WO2014096259A1 (en) | 2012-12-21 | 2014-06-26 | Novozymes A/S | Polypeptides having protease activiy and polynucleotides encoding same |
| WO2014122161A2 (en) | 2013-02-06 | 2014-08-14 | Novozymes A/S | Polypeptides having protease activity |
| US10619145B2 (en) | 2014-11-04 | 2020-04-14 | Novozymes A/S | Polypeptides having serine protease activity and polynucleotides encoding same and their application in animal feed |
| US11639522B2 (en) * | 2015-01-30 | 2023-05-02 | President And Fellows Of Harvard College | Microscope-free imaging |
| DE102016204815A1 (de) * | 2016-03-23 | 2017-09-28 | Henkel Ag & Co. Kgaa | Proteasen mit verbesserte Enzymstabilität in Waschmittel |
| DE102016204814A1 (de) * | 2016-03-23 | 2017-09-28 | Henkel Ag & Co. Kgaa | Verbesserte Reinigungsleistung an Protein sensitiven Anschmutzungen |
| JP7116062B2 (ja) | 2017-01-10 | 2022-08-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 多重化シグナル増幅 |
| MX386582B (es) | 2017-03-15 | 2025-03-19 | Int N&H Denmark Aps | Métodos para usar una serina proteasa de arqueas. |
| CN111630179B (zh) | 2018-01-26 | 2024-07-19 | 哈佛学院院长及董事 | 邻近检测方法和组合物 |
| US12142104B2 (en) | 2018-03-22 | 2024-11-12 | President And Fellows Of Harvard College | Methods and compositions for molecular authentication |
| CN110727780A (zh) * | 2019-10-17 | 2020-01-24 | 福建天晴数码有限公司 | 一种相识文本自动扩展的系统及其方法 |
| CN115074345B (zh) * | 2021-03-10 | 2024-04-05 | 中国农业科学院农业资源与农业区划研究所 | 耐热β-1,4-内切木聚糖酶及其编码基因与应用 |
| CA3257053A1 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | COMPOSITIONS AND METHODS FOR THE PREVENTION, TREATMENT, SUPPRESSION AND/OR ELIMINATION OF PHYTOPATHOGENIC INFESTATIONS AND INFECTIONS |
| CN115772531B (zh) * | 2022-11-11 | 2024-11-12 | 吉林农业大学 | 一种果胶酸裂解酶基因突变体及其克隆方法和应用 |
| CN116103264B (zh) * | 2022-12-28 | 2025-05-09 | 厦门蓝湾科技有限公司 | 一种提高磷脂酶d合成ps活性的理性定向进化方法 |
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-
2004
- 2004-10-08 ES ES04762907T patent/ES2358092T3/es not_active Expired - Lifetime
- 2004-10-08 EP EP10182980A patent/EP2308966A1/en not_active Ceased
- 2004-10-08 US US10/574,554 patent/US7892808B2/en active Active
- 2004-10-08 JP JP2006529655A patent/JP2007509603A/ja active Pending
- 2004-10-08 BR BRPI0414959-9A patent/BRPI0414959A/pt not_active Application Discontinuation
- 2004-10-08 CN CNA2004800297722A patent/CN1867668A/zh active Pending
- 2004-10-08 DE DE602004030595T patent/DE602004030595D1/de not_active Expired - Lifetime
- 2004-10-08 AU AU2004279924A patent/AU2004279924B2/en not_active Ceased
- 2004-10-08 EP EP04762907A patent/EP1673451B1/en not_active Expired - Lifetime
- 2004-10-08 AT AT04762907T patent/ATE491788T1/de not_active IP Right Cessation
- 2004-10-08 CA CA002539693A patent/CA2539693A1/en not_active Abandoned
- 2004-10-08 WO PCT/DK2004/000688 patent/WO2005035747A1/en not_active Ceased
- 2004-10-08 EP EP10182999A patent/EP2284259A3/en not_active Withdrawn
-
2011
- 2011-01-05 US US12/984,826 patent/US8377677B2/en not_active Expired - Lifetime
-
2012
- 2012-01-27 JP JP2012014730A patent/JP2012095666A/ja active Pending
-
2013
- 2013-01-10 US US13/738,490 patent/US8772011B2/en not_active Expired - Lifetime
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- 2014-05-23 US US14/286,089 patent/US9131711B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1673451A1 (en) | 2006-06-28 |
| DE602004030595D1 (de) | 2011-01-27 |
| US9131711B2 (en) | 2015-09-15 |
| EP2284259A3 (en) | 2011-06-15 |
| CN1867668A (zh) | 2006-11-22 |
| JP2012095666A (ja) | 2012-05-24 |
| EP1673451B1 (en) | 2010-12-15 |
| AU2004279924A1 (en) | 2005-04-21 |
| US20140255550A1 (en) | 2014-09-11 |
| US20090047387A1 (en) | 2009-02-19 |
| US8772011B2 (en) | 2014-07-08 |
| CA2539693A1 (en) | 2005-04-21 |
| JP2007509603A (ja) | 2007-04-19 |
| AU2004279924B2 (en) | 2010-09-30 |
| US20110097445A1 (en) | 2011-04-28 |
| ES2358092T3 (es) | 2011-05-05 |
| ATE491788T1 (de) | 2011-01-15 |
| US8377677B2 (en) | 2013-02-19 |
| WO2005035747A1 (en) | 2005-04-21 |
| EP2284259A2 (en) | 2011-02-16 |
| EP2308966A1 (en) | 2011-04-13 |
| US20130189732A1 (en) | 2013-07-25 |
| US7892808B2 (en) | 2011-02-22 |
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