DK200401821A - Fremgangsmåde til in vitro molekylær udvikling af proteinfunktion - Google Patents
Fremgangsmåde til in vitro molekylær udvikling af proteinfunktion Download PDFInfo
- Publication number
- DK200401821A DK200401821A DK200401821A DKPA200401821A DK200401821A DK 200401821 A DK200401821 A DK 200401821A DK 200401821 A DK200401821 A DK 200401821A DK PA200401821 A DKPA200401821 A DK PA200401821A DK 200401821 A DK200401821 A DK 200401821A
- Authority
- DK
- Denmark
- Prior art keywords
- stranded polynucleotide
- population
- reaction
- polynucleotide molecules
- parent
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract description 39
- 238000000338 in vitro Methods 0.000 title abstract 2
- 230000004853 protein function Effects 0.000 title abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract description 76
- 102000040430 polynucleotide Human genes 0.000 abstract description 76
- 108091033319 polynucleotide Proteins 0.000 abstract description 76
- 108060002716 Exonuclease Proteins 0.000 abstract description 17
- 102000013165 exonuclease Human genes 0.000 abstract description 17
- 239000011541 reaction mixture Substances 0.000 abstract description 14
- 239000012634 fragment Substances 0.000 abstract description 12
- 230000029087 digestion Effects 0.000 abstract description 7
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
- 108090000623 proteins and genes Proteins 0.000 abstract description 6
- 239000000203 mixture Substances 0.000 abstract description 4
- 238000000137 annealing Methods 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 description 15
- 102000004196 processed proteins & peptides Human genes 0.000 description 15
- 108090000765 processed proteins & peptides Proteins 0.000 description 15
- 239000003814 drug Substances 0.000 description 4
- 238000002703 mutagenesis Methods 0.000 description 3
- 231100000350 mutagenesis Toxicity 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 2
- 230000001079 digestive effect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003937 drug carrier Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000008194 pharmaceutical composition Substances 0.000 description 2
- 238000002560 therapeutic procedure Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 108010007577 Exodeoxyribonuclease I Proteins 0.000 description 1
- 108010046914 Exodeoxyribonuclease V Proteins 0.000 description 1
- 102100029075 Exonuclease 1 Human genes 0.000 description 1
- 102000019236 Exonuclease V Human genes 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 108010068698 spleen exonuclease Proteins 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biophysics (AREA)
- Bioinformatics & Computational Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Ecology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
reaktionsvolumenet, varigheden af fordøjelsesreaktionen, temperaturen af reaktionsblandingen, pH af reaktionsblandingen, længden af de enkeltstrengede moder-polynukleotidsekvenser, mængden af enkeltstrengede polynukleotidmolekyler og puffersammensætningen af reaktionsblandingen.
PATENTKRAV 1. Fremgangsmåde til frembringelse af en polynukleotidsekvens eller population af sekvenser ud fra enkeltstrengede moderpolynukleotidsekvenser, og som indkoder én eller flere proteinmotiver, idet fremgangsmåden omfatter trinnene, hvor man a) tiivejebringer en første population af enkeltstrengede polynukleotid-molekyler og en nummer to population af enkeltstrengede polynukleotid-molekyier, idet den første og nummer to populationen tilsammen udgør plus- og minusstrenge af moderpolynukleotidsekvenser; b) udfører en reaktion til fordøjelse af den første og nummer to populationen af enkeltstrengede polynukleotidmolekyler med en exonuklease for at frembringe tilsvarende populationer af enkeltstrengede polynukleotidfrag-menter; c) bringer de polynukleotidfragmenter, der er frembragt fra plusstrengene, i berøring med fragmenter, der er frembragt fra minusstrengene; og d) formerer de fragmenter, som hybridiserer til hinanden for at frembringe i det mindste én polynukleotidsekvens, der indkoder én eller flere protein-motiver, som har ændrede egenskaber sammenlignet med det eller de proteinmotiver, som indkodes af moderpolynukleotiderne, hvorved der i trin b) i det mindste er én parameter i den reaktion, der anvendes til fordøjelse af den første population af enkeltstrengede polynukleotidmolekyler, der er forskellig fra den eller de ækvivalente parametre, der anvendes i reaktionen til fordøjelse af nummer to populationen af enkeltstrengede polynukleotidmolekyler. 2. Fremgangsmåde ifølge krav 1, hvorved reaktionsparameteren vælges blandt exonukleasetypen, exonukleasekoncentrationen, reaktionsvolumenet, varigheden af fordøjelsesreaktionen, temperaturen af reaktionsblandingen, pH af reaktionsblandingen, længden af de enkeltstrengede moderpolynukleotidsekvenser, mængden af enkeltstrengede polynukleotidmolekyler og puffersammensætningen af reaktionsblandingen. 3. Fremgangsmåde ifølge krav 1 eller 2, hvorved den exonuklease, der anvendes til fordøjelse af den første population af enkeltstrengede polynukleotidmolekyler, er forskellig fra den exonuklease, der anvendes til fordøjelse af nummer to populationen af enkeltstrengede polynukleotidmolekyler. 4. Fremgangsmåde ifølge krav 3, hvorved den exonuklase, der anvendes til fordøjelse af den første population af enkeltstrengede polynukleotidmolekyler, er en 3'-exo-nuklease og den exonuklease, der anvendes til fordøjelse af nummer to populationen af enkeltstrengede polynukleotidmolekyler er en 5'-exonuklease. 5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved den exonukleasekoncentration der anvendes til fordøjelse af den første population af enkeitstrengede polynukleotidmolekyler, er forskellig fra den exonukleasekoncentration, der anvendes til fordøjelse af nummer to populationen af enkeltstrengede polynukleotidmolekyler. 6. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved det reaktionsvolumen, der anvendes til fordøjelse af den første population af enkeltstrengede polynukleotidmolekyler, er forskellig fra det reaktionsvolumen, der anvendes til fordøjelse af nummer to populationen af enkeltstrengede polynukleotidmolekyler. 7. Fremgangsmåde ifølge et hvilket som helst af de fregående krav, hvorved varigheden af den fordøjelsesreaktion, der anvendes til fordøjelsen af den første population af enkeltstrengede polynukleotidmolekyler. er forskellig fra varigheden af den fordøjelsesreaktion, der anvendes til fordøjelse af nummer to populationen af enkeltstrengede polynukleotidmolekyler. 8. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved temperaturen af den reaktionsblanding der anvendes til fordøjelse af den første population af enkeltstrengede polynukleotidmolekyler, er forskellig fra temperaturen af den reaktionsblanding, der anvendes til fordøjelse af nummer to populationen af enkeltstrengede polynukleotidmolekyler. 9. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved pH af den reaktionsblanding, der anvendes ti! fordøjelse af den første population af enkeltstrengede polynukleotidmolekyler, er forskellig fra pH-værdien af den reaktions blanding, der anvendes til fordøjelse af nummer to populationen af enkeltstrengede polynukleotidmolekyler. 10. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved længden af polynukleotiderne i den første population af enkeltstrengede polynukleotidmolekyler er forskellig fra længden af polynukleotiderne i nummer to populationen af enkeltstrengede polynukleotidmolekyler. 11. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved puffersammensætningen af den reaktionsblanding, der anvendes til fordøjelse af den første population af enkeltstrengede polynukleotidmolekyler, er forskellig fra puffer-sammensætningen af den reaktionsblanding, der anvendes til fordøjelse af nummer to populationen af enkeltstrengede polynukleotidmolekyler. 12. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved mængden af enkeltstrengede polynukleotidmolekyler i den første population af enkeltstrengede polynukleotidmolekyler er forskellig fra mængden af enkeltstrengede polynukleotidmolekyler i nummer to populationen af enkeltstrengede polynukleotidmolekyler. 13. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved den første population af enkeltstrengede polynukleotidmolekyler udgør plusstrenge af moderpolynukleotidsekvenser, og nummer to populationen af enkeltstrengede polynukleotidmolekyler udgør minusstrenge af moderpolynukleotidsekvenser, 14. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved polynukleotidmolekylerne i trin a) er DNA-molekyler. 15. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved trin c) yderligere omfatter, at man tilsætter primersekvenser, som hybridisere til 3'- og/eller 5'-enderne af i det mindste et af moderpolynukleotiderne under hybridiseringsbetingelser. 16. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved den exonuklease, der anvendes til at fordøje den første og/eller nummre to populationen af enkeltstrengede polynukleotidmolekyler er valgt fra gruppen bestående af BAL31,
Exonuklease I, Exonuklease V, Exonuklease VII, Exonuklease T7gen6, bakteriofag lambda exonuklease og Exonuklease Rec Jf. 17. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved en moderpolynukleotidsekvens eller -sekvenser underkastes mutagenese. 18. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved én eller begge af populationen af fragmenter, der er frembragt i trin b), underkastes mutagenese. 19. Fremgangsmåde ifølge krav 17 eller 18, hvorved mutagenesen er fejltilbøjelig PCR. 20. Fremgangsmåde ifølge et hvilket som helst af de foreågende krav, hvorved trin b) udføres for at frembringe populationer af enkeltstrengede fragmenter med varierende længder. 21. Fremgangsmåde ifølge krav 20, hvorved trin b) reguleres for at frembringe en population af enkeltstrengede fragmenter med en genemsnitiig længde på mere end omkring 50 nukleotider. 22. Fremgangsmåde ifølge et hvilket som helst af de foregående krav og som yderligere omfatter det trin, hvor man eksprimerer i det mindste én polynukleo-tidsekvens, der er frembragt i trin d), for at frembringe det indkodede polypeptid. 23. Fremgangsmåde ifølge krav 22 og som yderligere omfatter trinnet, hvor man tester det indkodede polypeptid for ønskede egenskaber. 24. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved moderpolynukleotidsekvensen indkoder et antistof eller fragment deraf. 25. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved moderpolynukleotidsekvensen indkoder et enzym. 26. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvorved moderpolynukleotidsekvensen indkoder et antigen. 27. Fremgangsmåde til fremstilling af et polypeptid med ønskede egenskaber, idet fremgangsmåden omfatter de følgende trin: a) frembringelse af forskellige former af et moderpolynukleotid ved anvendelse af en fremgangsmåde ifølge et hvilket som helst af kravene 1 til 26; b) eksprimerer de forskellige polynukleotider, der er frembragt i trin a), for at frembringe forskellige polypeptider; c) screening af de forskellige polypeptider for ønskede egenskaber; og d) udvælgelse af et polypeptid med ønskede egenskaber fra de forskellige polypeptider. 28. Polypeptid opnået ved en fremgangsmåde ifølge krav 27. 29. Farmaceutisk sammensætning omfattende et polypeptid ifølge krav 28 og en farmaceutisk acceptabel bærer. 30. Polypeptid ifølge krav 28 til anvendelse i medicin. 31. Anvendelse af et polypeptid ifølge krav 28 i fremstillingen af et lægemiddel til behandling, terapi og/eller diagnose afen sygdom. 32. Fremgangsmåde til fremstilling af en farmaceutisk sammensætning, hvilken fremgangsmåde omfatter, at man efter identifikationen af et polynukleotid og/eller et indkodet polypeptid med ønskede egenskaber ved en fremgangsmåde ifølge et hvilket som helst af kravene 1 til 26, tilsætter polynukleotidet og/eller det indkodede polypeptid til en farmaceutisk acceptabel bærer. 33. Fremgangsmåde som omfatter, at man efter identifikation af et polynukleotid og/eller et indkodet polypeptid med ønskede egenskaber ved en fremgangsmåde ifølge et hvilket som helst af kravene 1 til 26, anvender dette polynukleotid og/eller indkodede polypeptid helt eller delvis inden for medicinen. 34. Fremgangsmåpde ifølge krav 33, hvorved anvendelsen inde for medicinen er i behandlingen, terapien og/eller diagnosen af en sygdom. 35. Fremgangsmåde som omfatter, at man efter identifikation af et polynukleotid med ønskede egenskaber ifølge en fremgangsmåde ifølge et hvilket som helst af kravene 1 til 26, anvender dette polynukleotid til påvisning af og/eller formering af et målpolynukleotid i en prøve.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0211369A GB2388604B (en) | 2002-05-17 | 2002-05-17 | A method for in vitro molecular evolution of protein function |
| US10/321,195 US7153655B2 (en) | 1998-06-16 | 2002-12-17 | Method for in vitro molecular evolution of protein function involving the use of exonuclease enzyme and two populations of parent polynucleotide sequence |
| PCT/GB2003/002102 WO2003097834A2 (en) | 2002-05-17 | 2003-05-16 | A method for in vitro molecular evolution of protein function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK200401821A true DK200401821A (da) | 2004-11-23 |
Family
ID=29551433
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK03722867.3T DK1504098T4 (da) | 2002-05-17 | 2003-05-16 | Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion |
| DK200401821A DK200401821A (da) | 2002-05-17 | 2004-11-23 | Fremgangsmåde til in vitro molekylær udvikling af proteinfunktion |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK03722867.3T DK1504098T4 (da) | 2002-05-17 | 2003-05-16 | Fremgangsmåde til molekyulær udvikling in vitro af proteinfunktion |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7262012B2 (da) |
| EP (1) | EP1504098B2 (da) |
| JP (1) | JP4372679B2 (da) |
| AT (1) | ATE356871T1 (da) |
| AU (1) | AU2003230027A1 (da) |
| CA (1) | CA2485506C (da) |
| DE (1) | DE60312507T3 (da) |
| DK (2) | DK1504098T4 (da) |
| ES (1) | ES2285118T5 (da) |
| WO (1) | WO2003097834A2 (da) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2270609T3 (es) * | 1998-07-10 | 2007-04-01 | Alligator Bioscience Ab (Publ) | Proteina inhibidora de quimotaxis de staphylococcus y su uso. |
| EP1118663A1 (en) * | 2000-01-07 | 2001-07-25 | Universiteit Utrecht | Nucleic acids encoding chemotaxis inhibitory polypeptides |
| US20020086292A1 (en) | 2000-12-22 | 2002-07-04 | Shigeaki Harayama | Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules |
| EP1586583A3 (en) * | 2004-04-16 | 2005-11-16 | Alligator Bioscience AB (publ) | Compounds that block C5a complement receptor and their use in therapy |
| GB2432366B (en) * | 2005-11-19 | 2007-11-21 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
| GB0607798D0 (en) | 2006-04-20 | 2006-05-31 | Alligator Bioscience Ab | Novel polypeptides and use thereof |
| GB0708376D0 (en) | 2007-05-01 | 2007-06-06 | Alligator Bioscience Ab | Novel polypeptides and uses thereof |
| GB0905790D0 (en) | 2009-04-03 | 2009-05-20 | Alligator Bioscience Ab | Novel polypeptides and use thereof |
| GB0905503D0 (en) * | 2009-03-31 | 2009-05-13 | Alligator Bioscience Ab | A method for in vitro molecular evolution of protein function |
| GB0920258D0 (en) | 2009-11-19 | 2010-01-06 | Alligator Bioscience Ab | New medical agents and use thereof |
| GB201019086D0 (en) | 2010-11-11 | 2010-12-29 | Imp Innovations Ltd | Bacterial methods |
| EP2681302A2 (en) | 2011-03-04 | 2014-01-08 | Evolvemol, Inc. | Apparatus and process for isolating specific physical items within a set of physical items |
| GB201115280D0 (en) | 2011-09-05 | 2011-10-19 | Alligator Bioscience Ab | Antibodies, uses and methods |
| GB201311475D0 (en) | 2013-06-27 | 2013-08-14 | Alligator Bioscience Ab | Polypeptides |
| SG11201701070WA (en) | 2014-08-12 | 2017-03-30 | Alligator Bioscience Ab | Combination therapies with anti cd40 antibodies |
| KR102770238B1 (ko) | 2015-09-30 | 2025-02-18 | 얀센 바이오테크 인코포레이티드 | 인간 cd40에 특이적으로 결합하는 작용성 항체 및 사용 방법 |
| CN113892654A (zh) * | 2021-10-21 | 2022-01-07 | 长春大学 | 一种以绿豆蛋白粉为原料制备免消化绿豆蛋白质的方法 |
Family Cites Families (151)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4888286A (en) | 1984-02-06 | 1989-12-19 | Creative Biomolecules, Inc. | Production of gene and protein analogs through synthetic gene design using double stranded synthetic oligonucleotides |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| DE3590766T (da) | 1985-03-30 | 1987-04-23 | ||
| US6492107B1 (en) | 1986-11-20 | 2002-12-10 | Stuart Kauffman | Process for obtaining DNA, RNA, peptides, polypeptides, or protein, by recombinant DNA technique |
| US4994368A (en) | 1987-07-23 | 1991-02-19 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
| AU634186B2 (en) | 1988-11-11 | 1993-02-18 | Medical Research Council | Single domain ligands, receptors comprising said ligands, methods for their production, and use of said ligands and receptors |
| WO1990007936A1 (en) | 1989-01-23 | 1990-07-26 | Chiron Corporation | Recombinant therapies for infection and hyperproliferative disorders |
| US5043272A (en) | 1989-04-27 | 1991-08-27 | Life Technologies, Incorporated | Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers |
| CA2016842A1 (en) | 1989-05-16 | 1990-11-16 | Richard A. Lerner | Method for tapping the immunological repertoire |
| US5023171A (en) | 1989-08-10 | 1991-06-11 | Mayo Foundation For Medical Education And Research | Method for gene splicing by overlap extension using the polymerase chain reaction |
| GB8919607D0 (en) | 1989-08-30 | 1989-10-11 | Wellcome Found | Novel entities for cancer therapy |
| WO1991007506A1 (en) | 1989-11-08 | 1991-05-30 | The United States Of America, Represented By The Secretary, United States Department Of Commerce | A method of synthesizing double-stranded dna molecules |
| US5573907A (en) | 1990-01-26 | 1996-11-12 | Abbott Laboratories | Detecting and amplifying target nucleic acids using exonucleolytic activity |
| ES2078518T3 (es) | 1990-04-05 | 1995-12-16 | Roberto Crea | Mutagenesis por desplazamiento completo. |
| EP0528881A4 (en) | 1990-04-24 | 1993-05-26 | Stratagene | Methods for phenotype creation from multiple gene populations |
| US5387505A (en) | 1990-05-04 | 1995-02-07 | Eastman Kodak Company | Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage |
| GB9015198D0 (en) | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
| US5858725A (en) | 1990-10-10 | 1999-01-12 | Glaxo Wellcome Inc. | Preparation of chimaeric antibodies using the recombinant PCR strategy |
| DE69229222D1 (de) | 1991-03-07 | 1999-06-24 | Bradbury | Auswahl spezifischer proteine durch ein biologisches verfahren |
| DE4112440C1 (da) | 1991-04-16 | 1992-10-22 | Diagen Institut Fuer Molekularbiologische Diagnostik Gmbh, 4000 Duesseldorf, De | |
| US5512463A (en) | 1991-04-26 | 1996-04-30 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction library mutagenesis |
| US5514568A (en) | 1991-04-26 | 1996-05-07 | Eli Lilly And Company | Enzymatic inverse polymerase chain reaction |
| US5858657A (en) | 1992-05-15 | 1999-01-12 | Medical Research Council | Methods for producing members of specific binding pairs |
| US5871907A (en) | 1991-05-15 | 1999-02-16 | Medical Research Council | Methods for producing members of specific binding pairs |
| US5223408A (en) | 1991-07-11 | 1993-06-29 | Genentech, Inc. | Method for making variant secreted proteins with altered properties |
| WO1993006213A1 (en) | 1991-09-23 | 1993-04-01 | Medical Research Council | Production of chimeric antibodies - a combinatorial approach |
| IL103059A0 (en) | 1991-09-30 | 1993-02-21 | Boehringer Ingelheim Int | Conjugates for introducing nucleic acid into higher eucaryotic cells |
| US5521291A (en) | 1991-09-30 | 1996-05-28 | Boehringer Ingelheim International, Gmbh | Conjugates for introducing nucleic acid into higher eucaryotic cells |
| US5733731A (en) | 1991-10-16 | 1998-03-31 | Affymax Technologies N.V. | Peptide library and screening method |
| US5270170A (en) | 1991-10-16 | 1993-12-14 | Affymax Technologies N.V. | Peptide library and screening method |
| US5252479A (en) | 1991-11-08 | 1993-10-12 | Research Corporation Technologies, Inc. | Safe vector for gene therapy |
| AU3274793A (en) | 1991-12-12 | 1993-07-19 | Hybritech Incorporated | Enzymatic inverse polymerase chain reaction library mutagenesis |
| US5395750A (en) | 1992-02-28 | 1995-03-07 | Hoffmann-La Roche Inc. | Methods for producing proteins which bind to predetermined antigens |
| WO1994012632A1 (en) | 1992-11-27 | 1994-06-09 | University College London | Improvements in nucleic acid synthesis by pcr |
| US5733753A (en) | 1992-12-22 | 1998-03-31 | Novo Nordisk A/S | Amplification of genomic DNA by site specific integration of a selectable marker construct |
| US5491074A (en) | 1993-04-01 | 1996-02-13 | Affymax Technologies Nv | Association peptides |
| SE9304060D0 (sv) | 1993-12-06 | 1993-12-06 | Bioinvent Int Ab | Sätt att selektera specifika bakteriofager |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6406855B1 (en) | 1994-02-17 | 2002-06-18 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US6395547B1 (en) | 1994-02-17 | 2002-05-28 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5834252A (en) | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
| US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6165793A (en) | 1996-03-25 | 2000-12-26 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US6309883B1 (en) | 1994-02-17 | 2001-10-30 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5928905A (en) | 1995-04-18 | 1999-07-27 | Glaxo Group Limited | End-complementary polymerase reaction |
| US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
| WO1996017056A1 (en) | 1994-12-02 | 1996-06-06 | Institut Pasteur | Hypermutagenesis |
| US5869451A (en) | 1995-06-07 | 1999-02-09 | Glaxo Group Limited | Peptides and compounds that bind to a receptor |
| ATE390439T1 (de) | 1995-06-07 | 2008-04-15 | Glaxo Group Ltd | An einem thrombopoietin rezeptor bindende peptide und zusammensetzungen |
| WO1997007205A1 (en) | 1995-08-11 | 1997-02-27 | Novo Nordisk A/S | Method for preparing polypeptide variants |
| DK1143006T3 (da) | 1995-08-18 | 2008-07-14 | Morphosys Ip Gmbh | Vektorer/DNA-sekvenser fra humane kombinatoriske antistofbiblioteker |
| US6479258B1 (en) | 1995-12-07 | 2002-11-12 | Diversa Corporation | Non-stochastic generation of genetic vaccines |
| US6489145B1 (en) | 1996-07-09 | 2002-12-03 | Diversa Corporation | Method of DNA shuffling |
| US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
| US6361974B1 (en) | 1995-12-07 | 2002-03-26 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
| WO2000058517A1 (en) | 1999-03-26 | 2000-10-05 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
| US6368798B1 (en) | 1995-12-07 | 2002-04-09 | Diversa Corporation | Screening for novel bioactivities |
| US6537776B1 (en) | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
| US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
| US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
| US6238884B1 (en) | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
| US6352842B1 (en) | 1995-12-07 | 2002-03-05 | Diversa Corporation | Exonucease-mediated gene assembly in directed evolution |
| US6358709B1 (en) | 1995-12-07 | 2002-03-19 | Diversa Corporation | End selection in directed evolution |
| US6171820B1 (en) | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
| US6096548A (en) | 1996-03-25 | 2000-08-01 | Maxygen, Inc. | Method for directing evolution of a virus |
| US5851804A (en) | 1996-05-06 | 1998-12-22 | Apollon, Inc. | Chimeric kanamycin resistance gene |
| US5877001A (en) | 1996-06-17 | 1999-03-02 | Diverso Corporation | Amidase |
| US5763239A (en) | 1996-06-18 | 1998-06-09 | Diversa Corporation | Production and use of normalized DNA libraries |
| JPH1066576A (ja) | 1996-08-07 | 1998-03-10 | Novo Nordisk As | 突出末端を有する２本鎖ｄｎａ及びこれを用いたｄｎａのシャフリング方法 |
| AU4597197A (en) | 1996-09-27 | 1998-04-17 | Introgene B.V. | Methods for optimization of gene therapy by recursive sequence shuffling and selection |
| WO1998013487A1 (en) | 1996-09-27 | 1998-04-02 | Maxygen, Inc. | Methods for optimization of gene therapy by recursive sequence shuffling and selection |
| US5925544A (en) | 1996-11-18 | 1999-07-20 | Novo Nordisk A/S | Method of homologous recombination followed by in vivo selection of DNA amplification |
| WO1998028416A1 (en) | 1996-12-20 | 1998-07-02 | Novo Nordisk A/S | In vivo recombination |
| AU743305C (en) | 1997-01-17 | 2006-03-30 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| WO1998031816A1 (en) | 1997-01-17 | 1998-07-23 | Regents Of The University Of Minnesota | Dna molecules and protein displaying improved triazine compound degrading ability |
| US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
| GB9701425D0 (en) | 1997-01-24 | 1997-03-12 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
| DK1015575T3 (da) | 1997-03-18 | 2010-08-23 | Novozymes As | Shuffling af heterologe DNA-sekvenser |
| US6159688A (en) | 1997-03-18 | 2000-12-12 | Novo Nordisk A/S | Methods of producing polynucleotide variants |
| DE69840382D1 (de) | 1997-03-18 | 2009-02-05 | Novozymes As | Methode zur herstellung einer bibliothek mittels dna-shuffling |
| DK0973940T3 (da) | 1997-03-18 | 2008-10-20 | Novozymes As | En in vitro-fremgangsmåde til konstruktion af et DNA-bibliotek |
| US6159687A (en) | 1997-03-18 | 2000-12-12 | Novo Nordisk A/S | Methods for generating recombined polynucleotides |
| US6291165B1 (en) | 1997-03-18 | 2001-09-18 | Novo Nordisk A/S | Shuffling of heterologous DNA sequences |
| US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
| GB9712512D0 (en) | 1997-06-16 | 1997-08-20 | Bioinvent Int Ab | A method for in vitro molecular evolution of protein function |
| WO1999021979A1 (en) | 1997-10-28 | 1999-05-06 | Maxygen, Inc. | Human papillomavirus vectors |
| EP1030861A4 (en) | 1997-10-31 | 2001-09-05 | Maxygen Inc | MODIFICATION OF VIRUSTROPISMIUS AND THE ECONOMIC SPECTRUM BY VIRAL GENOM MIXING |
| WO1999033965A1 (en) | 1997-12-24 | 1999-07-08 | Diatech Pty. Ltd. | Bifunctional molecules |
| WO1999041383A1 (en) | 1998-02-11 | 1999-08-19 | Maxygen, Inc. | Antigen library immunization |
| WO1999041402A2 (en) | 1998-02-11 | 1999-08-19 | Maxygen, Inc. | Targeting of genetic vaccine vectors |
| US20010006950A1 (en) | 1998-02-11 | 2001-07-05 | Juha Punnonen | Genetic vaccine vector engineering |
| AUPP221098A0 (en) | 1998-03-06 | 1998-04-02 | Diatech Pty Ltd | V-like domain binding molecules |
| WO1999057128A1 (en) | 1998-05-01 | 1999-11-11 | Maxygen, Inc. | Optimization of pest resistance genes using dna shuffling |
| US6562622B1 (en) | 1998-05-08 | 2003-05-13 | Diatech Pty, Ltd | Continuous in vitro evolution |
| IL140125A0 (en) | 1998-06-17 | 2002-02-10 | Maxygen Inc | Method for producing polynucleotides with desired properties |
| US6365408B1 (en) | 1998-06-19 | 2002-04-02 | Maxygen, Inc. | Methods of evolving a polynucleotides by mutagenesis and recombination |
| US20020059659A1 (en) | 1998-08-12 | 2002-05-16 | Maxygen, Inc. | DNA shuffling to produce herbicide selective crops |
| FR2782323B1 (fr) | 1998-08-12 | 2002-01-11 | Proteus | Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues |
| KR20010083077A (ko) | 1998-08-12 | 2001-08-31 | 추후제출 | 제초제 선택성 작물을 생성하는 ｄｎａ 재편성 |
| WO2000012680A1 (en) | 1998-08-31 | 2000-03-09 | Maxygen, Inc. | Transformation, selection, and screening of sequence-shuffled polynucleotides for development and optimization of plant phenotypes |
| IL140441A0 (en) | 1998-09-29 | 2002-02-10 | Maxygen Inc | Shuffling of codon altered genes |
| US6500639B2 (en) | 1998-10-07 | 2002-12-31 | Maxygen, Inc. | DNA shuffling to produce nucleic acids for mycotoxin detoxification |
| AU1720300A (en) | 1998-11-10 | 2000-05-29 | Maxygen, Inc. | Modified phosphoenolpyruvate carboxylase for improvement and optimization of plant phenotypes |
| EP1129184A1 (en) | 1998-11-10 | 2001-09-05 | Maxygen, Inc. | Modified adp-glucose pyrophosphorylase for improvement and optimation of plant phenotypes |
| EP1129182A1 (en) | 1998-11-10 | 2001-09-05 | Maxygen, Inc. | Modified ribulose 1,5-bisphosphate carboxylase/oxygenase |
| FR2786787B1 (fr) | 1998-12-08 | 2002-04-19 | Proteus | Methode d'analyse in vitro d'un phenotype connu a partir d'un echantillon d'acides nucleiques |
| US6358712B1 (en) | 1999-01-05 | 2002-03-19 | Trustee Of Boston University | Ordered gene assembly |
| US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
| US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| US6368861B1 (en) | 1999-01-19 | 2002-04-09 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
| AU2415200A (en) | 1999-01-18 | 2000-08-01 | Maxygen, Inc. | Methods of populating data structures for use in evolutionary simulations |
| ATE465459T1 (de) | 1999-01-19 | 2010-05-15 | Maxygen Inc | Durch oligonukleotide-vermittelte nukleinsäuren- rekombination |
| WO2000052155A2 (en) | 1999-03-05 | 2000-09-08 | Maxygen, Inc. | Recombination of insertion modified nucleic acids |
| EP1161529A2 (en) | 1999-03-09 | 2001-12-12 | Diversa Corporation | End selection in directed evolution |
| WO2000061740A1 (en) | 1999-04-10 | 2000-10-19 | Maxygen, Inc. | Modified lipid production |
| AU4456200A (en) | 1999-04-13 | 2000-11-14 | Maxygen, Inc. | Modified starch metabolism enzymes and encoding genes for improvement and optimization of plant phenotypes |
| US7332308B1 (en) | 1999-05-21 | 2008-02-19 | The Penn State Research Foundation | Incrementally truncated nucleic acids and methods of making same |
| AU5030900A (en) | 1999-05-21 | 2000-12-12 | Penn State Research Foundation, The | Construction of incremental truncation libraries |
| JP2003509010A (ja) | 1999-05-31 | 2003-03-11 | ノボザイムス アクティーゼルスカブ | ペプチドのスクリーニング方法 |
| AU5880900A (en) | 1999-06-25 | 2001-01-31 | Maxygen, Inc. | Methods and compositions for engineering of attenuated vaccines |
| WO2001002865A1 (en) | 1999-07-06 | 2001-01-11 | Maxygen, Inc. | Cyclotron mass spectrometry screening |
| WO2001004287A1 (en) | 1999-07-07 | 2001-01-18 | Maxygen Aps | A method for preparing modified polypeptides |
| EP1208193A1 (en) | 1999-08-12 | 2002-05-29 | Maxygen, Inc. | Dna shuffling of dioxygenase genes for production of industrial chemicals |
| WO2001025438A2 (en) | 1999-10-07 | 2001-04-12 | Maxygen, Inc. | Ifn-alpha homologues |
| EP1222304B1 (en) | 1999-10-13 | 2009-12-09 | The Board of Trustees of The Leland Stanford Junior University | Biosynthesis of polyketide synthase substrates |
| EP1230269A2 (en) | 1999-11-03 | 2002-08-14 | Maxygen, Inc. | Antibody diversity generation |
| DE19953854C2 (de) | 1999-11-09 | 2002-01-17 | Max Planck Gesellschaft | Verfahren zur Herstellung von Biopolymeren mit veränderten Eigenschaften |
| WO2001038504A2 (en) | 1999-11-23 | 2001-05-31 | Maxygen, Inc. | Homologous recombination in plants |
| WO2001038513A2 (en) | 1999-11-23 | 2001-05-31 | Maxygen, Inc. | Shuffling of agrobacterium and viral genes, plasmids and genomes for improved plant transformation |
| AU2257401A (en) | 1999-12-08 | 2001-06-18 | California Institute Of Technology | Directed evolution of biosynthetic and biodegration pathways |
| AU2594301A (en) | 1999-12-23 | 2001-07-03 | Maxygen, Inc. | Alteration of hydrolase genes and screening of the resulting libraries for the ability to catalyze specific reactions |
| JP2003519495A (ja) | 2000-01-11 | 2003-06-24 | マキシジェン， インコーポレイテッド | 多様性生成およびスクリーニングのための一体化されたシステムおよび方法 |
| US6329178B1 (en) | 2000-01-14 | 2001-12-11 | University Of Washington | DNA polymerase mutant having one or more mutations in the active site |
| AU2001241939A1 (en) | 2000-02-28 | 2001-09-12 | Maxygen, Inc. | Single-stranded nucleic acid template-mediated recombination and nucleic acid fragment isolation |
| AU2001243376A1 (en) | 2000-02-29 | 2001-09-12 | Maxygen, Inc. | Triazine degrading enzymes |
| WO2001068803A2 (en) | 2000-03-02 | 2001-09-20 | Maxygen, Inc. | Enzymes, pathways and organisms for making a polymerizable monomer by whole cell bioprocess |
| AU2001243670A1 (en) | 2000-03-20 | 2001-10-03 | Maxygen, Inc. | Method for generating recombinant dna molecules in complex mixtures |
| EP1276861A2 (en) | 2000-03-24 | 2003-01-22 | Maxygen, Inc. | Methods for modulating cellular and organismal phenotypes |
| EP1272967A2 (en) | 2000-03-30 | 2003-01-08 | Maxygen, Inc. | In silico cross-over site selection |
| ATE500323T1 (de) | 2000-04-03 | 2011-03-15 | Maxygen Inc | Subtilisin-variante |
| WO2001096551A2 (en) | 2000-06-14 | 2001-12-20 | Diversa Corporation | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
| WO2002004629A2 (en) | 2000-07-07 | 2002-01-17 | Maxygen, Inc. | Molecular breeding of transposable elements |
| AU2001279135A1 (en) | 2000-07-31 | 2002-02-13 | Maxygen, Inc. | Biosensors, reagents and diagnostic applications of directed evolution |
| FR2813314B1 (fr) | 2000-08-25 | 2004-05-07 | Biomethodes | Procede de mutagenese dirigee massive |
| US20020137889A1 (en) | 2000-09-18 | 2002-09-26 | Soong Nay Wei | Stress resistant retroviruses |
| WO2002029032A2 (en) | 2000-09-30 | 2002-04-11 | Diversa Corporation | Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating |
| US7560622B2 (en) | 2000-10-06 | 2009-07-14 | Pioneer Hi-Bred International, Inc. | Methods and compositions relating to the generation of partially transgenic organisms |
| JP3777158B2 (ja) | 2000-11-10 | 2006-05-24 | アミコージェン・インコーポレイテッド | 一方向性の一本鎖ｄｎａ切片を用いた組換えｄｎａライブラリーの製造方法 |
| JP4430303B2 (ja) | 2000-12-12 | 2010-03-10 | アリゲーター・バイオサイエンス・アーベー | タンパク質機能のインビトロ分子進化方法 |
| US6958213B2 (en) * | 2000-12-12 | 2005-10-25 | Alligator Bioscience Ab | Method for in vitro molecular evolution of protein function |
-
2003
- 2003-05-16 DE DE60312507T patent/DE60312507T3/de not_active Expired - Lifetime
- 2003-05-16 EP EP03722867A patent/EP1504098B2/en not_active Expired - Lifetime
- 2003-05-16 WO PCT/GB2003/002102 patent/WO2003097834A2/en not_active Ceased
- 2003-05-16 AU AU2003230027A patent/AU2003230027A1/en not_active Abandoned
- 2003-05-16 JP JP2004506491A patent/JP4372679B2/ja not_active Expired - Fee Related
- 2003-05-16 DK DK03722867.3T patent/DK1504098T4/da active
- 2003-05-16 AT AT03722867T patent/ATE356871T1/de active
- 2003-05-16 CA CA2485506A patent/CA2485506C/en not_active Expired - Fee Related
- 2003-05-16 US US10/514,399 patent/US7262012B2/en not_active Expired - Lifetime
- 2003-05-16 ES ES03722867T patent/ES2285118T5/es not_active Expired - Lifetime
-
2004
- 2004-11-23 DK DK200401821A patent/DK200401821A/da not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| WO2003097834A2 (en) | 2003-11-27 |
| EP1504098B1 (en) | 2007-03-14 |
| DK1504098T3 (da) | 2007-06-11 |
| EP1504098B2 (en) | 2011-02-09 |
| WO2003097834A3 (en) | 2004-02-19 |
| ATE356871T1 (de) | 2007-04-15 |
| ES2285118T5 (es) | 2012-09-21 |
| ES2285118T3 (es) | 2007-11-16 |
| DK1504098T4 (da) | 2011-05-23 |
| DE60312507D1 (de) | 2007-04-26 |
| AU2003230027A1 (en) | 2003-12-02 |
| CA2485506C (en) | 2012-02-28 |
| JP4372679B2 (ja) | 2009-11-25 |
| US7262012B2 (en) | 2007-08-28 |
| DE60312507T2 (de) | 2007-12-06 |
| CA2485506A1 (en) | 2003-11-27 |
| JP2005525820A (ja) | 2005-09-02 |
| EP1504098A2 (en) | 2005-02-09 |
| US20060166198A1 (en) | 2006-07-27 |
| DE60312507T3 (de) | 2011-08-18 |
| AU2003230027A8 (en) | 2003-12-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK200401821A (da) | Fremgangsmåde til in vitro molekylær udvikling af proteinfunktion | |
| EP3436581B1 (en) | Methods of whole transcriptome amplification | |
| KR102458022B1 (ko) | 혼합물 중 핵산의 서열분석 방법 및 그와 관련된 조성물 | |
| EP3027775B1 (en) | Dna sequencing and epigenome analysis | |
| US20100323404A1 (en) | Method for recombining dna sequences and compositions related thereto | |
| EP2396430A4 (en) | TEMPLATE INDEPENDENT LIGATION OF SINGLE-STRAND DNA | |
| EP3607065B1 (en) | Method and kit for constructing nucleic acid library | |
| EP2589657A9 (en) | Method for detection of target molecule | |
| EP2354243A1 (en) | Complexity reduction method | |
| CA2430582A1 (en) | A method for in vitro molecular evolution of protein function | |
| KR20170012390A (ko) | 시퀀싱 공정 | |
| CN109312391A (zh) | 生成用于单分子测序的单链环状dna文库的方法 | |
| US11339427B2 (en) | Method for target specific RNA transcription of DNA sequences | |
| US12378603B2 (en) | Nucleic acid sequencing methods and computer-readable media for practicing same | |
| JP6756835B2 (ja) | ウイルス性出血性敗血症ウイルスの遺伝子変異の検出方法 | |
| US20180291369A1 (en) | Error-proof nucleic acid library construction method and kit | |
| CN112301020A (zh) | Iii类脱氧核酶突变体及其制备方法与应用 | |
| CN113614228A (zh) | 使用poly(a)聚合酶对rna的尺寸选择 | |
| ATE516351T1 (de) | Verfahren zur molekularen evolution der proteinfunktion in vitro | |
| KR20240172319A (ko) | 구조가 변환된 덤벨형 템플릿 및 이를 이용한 회전환 반응 | |
| US20210115435A1 (en) | Error-proof nucleic acid library construction method | |
| CN115896243A (zh) | 基于混合酶体系的超灵敏反转录方法及其应用 | |
| CN107893120A (zh) | 检测运动基因snp的引物组及其应用和产品以及检测运动基因snp的检测方法及应用 | |
| EP4512906A1 (en) | Method for vector insertion site detection and clonal quantification using tagmentation | |
| US20250154501A1 (en) | Method for vector insertion site detection and clonal quantification using tagmentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| AHS | Application shelved for other reasons than non-payment |