DK1520012T3 - Monopropylenglycol tilsat til fermenteringsmedium - Google Patents

Monopropylenglycol tilsat til fermenteringsmedium

Info

Publication number
DK1520012T3
DK1520012T3 DK03761447T DK03761447T DK1520012T3 DK 1520012 T3 DK1520012 T3 DK 1520012T3 DK 03761447 T DK03761447 T DK 03761447T DK 03761447 T DK03761447 T DK 03761447T DK 1520012 T3 DK1520012 T3 DK 1520012T3
Authority
DK
Denmark
Prior art keywords
sub
fermentation medium
monopropylene glycol
glycol added
culture solution
Prior art date
Application number
DK03761447T
Other languages
English (en)
Inventor
Svend Kaasgaard
Niels Banke
Kim Uhre Hansen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK1520012T3 publication Critical patent/DK1520012T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Processing Of Solid Wastes (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DK03761447T 2002-07-01 2003-07-01 Monopropylenglycol tilsat til fermenteringsmedium DK1520012T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200201021 2002-07-01
PCT/DK2003/000455 WO2004003187A2 (en) 2002-07-01 2003-07-01 Mpg added to fermentation

Publications (1)

Publication Number Publication Date
DK1520012T3 true DK1520012T3 (da) 2009-04-27

Family

ID=29797022

Family Applications (1)

Application Number Title Priority Date Filing Date
DK03761447T DK1520012T3 (da) 2002-07-01 2003-07-01 Monopropylenglycol tilsat til fermenteringsmedium

Country Status (8)

Country Link
US (1) US20060127974A1 (da)
EP (1) EP1520012B1 (da)
CN (1) CN100529066C (da)
AT (1) ATE420161T1 (da)
AU (1) AU2003243925A1 (da)
DE (1) DE60325731D1 (da)
DK (1) DK1520012T3 (da)
WO (1) WO2004003187A2 (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006136164A1 (en) * 2005-06-24 2006-12-28 Novozymes A/S Mutant cells suitable for recombinant polypeptide production
CN100999742B (zh) * 2007-01-09 2011-05-11 清华大学 一种发酵生产1，3-丙二醇的高产工艺
US20110189344A1 (en) 2008-06-09 2011-08-04 Michael Bodo Recovery of insoluble enzyme from fermentation broth and formulation of insoluble enzyme
CN104480152B (zh) * 2014-11-20 2017-09-01 武汉华士特工业生物技术开发有限公司 一种提高裂殖壶菌油脂中二十二碳六烯酸含量的方法
JP2021504541A (ja) * 2017-11-29 2021-02-15 ビーエイエスエフ・ソシエタス・エウロパエアＢａｓｆ Ｓｅ 保存安定性酵素調製物、それらの製造およびそれらを使用する方法
EP3983425B1 (en) 2019-06-13 2025-10-01 Basf Se Method of recovering a protein from fermentation broth using a divalent cation

Family Cites Families (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3111459A (en) * 1959-10-19 1963-11-19 Ajinomoto Kk Method for preparation of inosine
US3222257A (en) * 1961-04-19 1965-12-07 Ajinomoto Kk Process for producing nucleosides by microorganisms
GB1001173A (en) * 1962-10-22 1965-08-11 Miles Lab Process for the production of galactose oxidase
GB1296839A (da) 1969-05-29 1972-11-22
GB1372034A (en) 1970-12-31 1974-10-30 Unilever Ltd Detergent compositions
US3951937A (en) * 1973-12-20 1976-04-20 The Community Blood Council Of Greater New York, Inc. Large scale purification of hepatitis type B antigen using polyethylene glycol
DE2512735A1 (de) * 1975-03-22 1976-09-30 Henkel & Cie Gmbh Verfahren zur gewinnung von proteinen aus waessrigen protein- loesungen
US4069216A (en) * 1975-06-16 1978-01-17 Edward Shanbrom, Inc. Simplified methods for preparation of very high purity Factor VIII concentrate
JPS5953038B2 (ja) * 1979-04-07 1984-12-22 メルシャン株式会社 サイクロデキストリンの製造法
DD153495A3 (de) * 1979-07-03 1982-01-13 Petrolchemisches Kombinat Hilfsstoffkombination zur verbesserung mikrobiologischer verfahren
DK187280A (da) 1980-04-30 1981-10-31 Novo Industri As Ruhedsreducerende middel til et fuldvaskemiddel fuldvaskemiddel og fuldvaskemetode
JPS6030682A (ja) * 1983-07-30 1985-02-16 Amano Pharmaceut Co Ltd β−アミラ−ゼの製造法
US4588691A (en) * 1984-02-29 1986-05-13 Johal Sarjit S Method for preparation of Fraction I protein and by-products thereof
US4673647A (en) * 1985-05-06 1987-06-16 Miles Laboratories, Inc. Process to solubilize enzymes and an enzyme liquid product produced thereby
WO1987000859A1 (en) 1985-08-09 1987-02-12 Gist-Brocades N.V. Novel lipolytic enzymes and their use in detergent compositions
US4728613A (en) * 1985-09-04 1988-03-01 Miles Laboratories, Inc. Method for the recovery of extracellular enzymes from whole fermentation beer
EP0258068B1 (en) 1986-08-29 1994-08-31 Novo Nordisk A/S Enzymatic detergent additive
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
DE3854249T2 (de) 1987-08-28 1996-02-29 Novonordisk As Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen.
JPS6474992A (en) 1987-09-16 1989-03-20 Fuji Oil Co Ltd Dna sequence, plasmid and production of lipase
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
EP0394352B1 (en) 1988-01-07 1992-03-11 Novo Nordisk A/S Enzymatic detergent
CH667673A5 (en) * 1988-01-22 1988-10-31 Eidgenoess Tech Hochschule Prodn. of fermentation broth with lignolytic activity - by growing fungi under nutrient limited conditions in stirred reactor and in presence of cell wall stabiliser
JP3079276B2 (ja) 1988-02-28 2000-08-21 天野製薬株式会社 組換え体ｄｎａ、それを含むシュードモナス属菌及びそれを用いたリパーゼの製造法
US5648263A (en) 1988-03-24 1997-07-15 Novo Nordisk A/S Methods for reducing the harshness of a cotton-containing fabric
JP2728531B2 (ja) 1988-03-24 1998-03-18 ノボ ノルディスク アクティーゼルスカブ セルラーゼ調製品
US5260202A (en) * 1988-09-07 1993-11-09 Delta Biotechnology Limited Fermentation method
GB8915658D0 (en) 1989-07-07 1989-08-23 Unilever Plc Enzymes,their production and use
DK0528828T4 (da) 1990-04-14 1998-08-31 Genencor Internat Gmbh Alkaliske bacillus-lipaser, DNA-sekvenser, der koder herfor, og bacilli der producerer sådanne lipaser
CA2082279C (en) 1990-05-09 2007-09-04 Grethe Rasmussen Cellulase preparation comprising an endoglucanase enzyme
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
DK0548228T3 (da) 1990-09-13 1999-05-10 Novo Nordisk As Lipasevarianter
DE69133035T2 (de) 1991-01-16 2003-02-13 The Procter & Gamble Company, Cincinnati Kompakte Waschmittelzusammensetzungen mit hochaktiven Cellulasen
ES2121014T3 (es) 1991-05-01 1998-11-16 Novo Nordisk As Enzimas estabilizadas y composiciones detergentes.
US5371198A (en) * 1991-12-16 1994-12-06 Novo Nordisk A/S Method for protection of proteolysis-susceptible protein during protein production in a fluid medium
EP0571014B1 (en) * 1992-05-18 2004-03-31 Genencor International, Inc. Bacteria producing alkaline proteases, and production of these alkaline proteases
DK88892D0 (da) 1992-07-06 1992-07-06 Novo Nordisk As Forbindelse
KR100294361B1 (ko) 1992-07-23 2001-09-17 피아 스타르 돌연변이체알파-아밀라제,세정제,접시세척제,및액화제
JP3254457B2 (ja) * 1992-09-18 2002-02-04 株式会社日立製作所 無給油式スクリュー圧縮機のロータ形成方法およびそのロータを用いた無給油式スクリュー圧縮機
US5792641A (en) 1992-10-06 1998-08-11 Novo Nordisk A/S Cellulase variants and detergent compositions containing cellulase variants
HU219675B (hu) 1993-02-11 2001-06-28 Genencor International Inc. Oxidatívan stabil alfa-amiláz
DE69434242T2 (de) 1993-04-27 2006-01-12 Genencor International, Inc., Palo Alto Neuartige Lipasevarianten zur Verwendung in Reinigungsmitteln
DK52393D0 (da) 1993-05-05 1993-05-05 Novo Nordisk As
US5443981A (en) * 1993-06-09 1995-08-22 Rutgers, The State University Acremonium typhinum ATCC 74228 which exhibits endoproteolytic activity
JP2859520B2 (ja) 1993-08-30 1999-02-17 ノボ ノルディスク アクティーゼルスカブ リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物
JPH07143883A (ja) 1993-11-24 1995-06-06 Showa Denko Kk リパーゼ遺伝子及び変異体リパーゼ
MX9603542A (es) 1994-02-22 1997-03-29 Novo Nordisk As Metodo para preparar una variante de una enzima lipolitica.
DE69536145D1 (de) 1994-03-08 2011-04-07 Novozymes As Neuartige alkalische Zellulasen
CA2186592C (en) 1994-03-29 2008-02-19 Helle Outtrup Alkaline bacillus amylase
WO1995030744A2 (en) 1994-05-04 1995-11-16 Genencor International Inc. Lipases with improved surfactant resistance
AU2884595A (en) 1994-06-20 1996-01-15 Unilever Plc Modified pseudomonas lipases and their use
AU2884695A (en) 1994-06-23 1996-01-19 Unilever Plc Modified pseudomonas lipases and their use
US5919691A (en) 1994-10-06 1999-07-06 Novo Nordisk A/S Enzyme and enzyme preparation with endoglucanase activity
BE1008998A3 (fr) 1994-10-14 1996-10-01 Solvay Lipase, microorganisme la produisant, procede de preparation de cette lipase et utilisations de celle-ci.
CN1167503A (zh) 1994-10-26 1997-12-10 诺沃挪第克公司 一种具有脂解活性的酶
AR000862A1 (es) 1995-02-03 1997-08-06 Novozymes As Variantes de una ó-amilasa madre, un metodo para producir la misma, una estructura de adn y un vector de expresion, una celula transformada por dichaestructura de adn y vector, un aditivo para detergente, composicion detergente, una composicion para lavado de ropa y una composicion para la eliminacion del
JPH08228778A (ja) 1995-02-27 1996-09-10 Showa Denko Kk 新規なリパーゼ遺伝子及びそれを用いたリパーゼの製造方法
CN1182451A (zh) 1995-03-17 1998-05-20 诺沃挪第克公司 新的内切葡聚糖酶
EP0839186B1 (en) 1995-07-14 2004-11-10 Novozymes A/S A modified enzyme with lipolytic activity
JP4068142B2 (ja) 1995-08-11 2008-03-26 ノボザイムス アクティーゼルスカブ 新規の脂肪分解酵素
US5763385A (en) 1996-05-14 1998-06-09 Genencor International, Inc. Modified α-amylases having altered calcium binding properties
US5616693A (en) * 1996-07-01 1997-04-01 Alpha Therapeutic Corporation Process for seperating alpha-1-proteinase inhibitor from COHN IV1 +1V4 paste
AU3938997A (en) 1996-08-26 1998-03-19 Novo Nordisk A/S A novel endoglucanase
BR9711479B1 (pt) 1996-09-17 2009-08-11 variante de celulase tendo uma resistência aumentada a tensìdeo de ánion.
AU4772697A (en) 1996-11-04 1998-05-29 Novo Nordisk A/S Subtilase variants and compositions
CN1530443A (zh) 1996-11-04 2004-09-22 ŵ����÷�����޹�˾ 枯草杆菌酶变异体和组合物
WO1998034946A1 (en) 1997-02-12 1998-08-13 Massachusetts Institute Of Technology Daxx, a novel fas-binding protein that activates jnk and apoptosis
PL335227A1 (en) 1997-02-20 2000-04-10 Dsm Nv Industrial-scale production of valuable compounds by fermentation in a chemically defined medium
EP1002061A1 (en) 1997-07-04 2000-05-24 Novo Nordisk A/S FAMILY 6 ENDO-1,4-$g(b)-GLUCANASE VARIANTS AND CLEANING COMPOSIT IONS CONTAINING THEM
EP1263942B1 (en) 2000-03-08 2013-11-06 Novozymes A/S Variants with altered properties
DE60215823T2 (de) * 2001-08-07 2007-09-06 Novozymes A/S Kohlhydrate und polyole zum lösen der proteinkristalle

Also Published As

Publication number Publication date
US20060127974A1 (en) 2006-06-15
ATE420161T1 (de) 2009-01-15
DE60325731D1 (en) 2009-02-26
EP1520012A2 (en) 2005-04-06
CN100529066C (zh) 2009-08-19
AU2003243925A8 (en) 2004-01-19
AU2003243925A1 (en) 2004-01-19
WO2004003187A3 (en) 2004-03-18
CN1665923A (zh) 2005-09-07
EP1520012B1 (en) 2009-01-07
WO2004003187A2 (en) 2004-01-08

Similar Documents

Publication Publication Date Title
BR9912875A (pt) Método para a recuperação de proteìnas a partir do fluido intersticial de tecidos vegetais
NO961812D0 (no) Humanproteaseinhibitorer av Kunitz-type
DK1007503T3 (da) Ny kemisk forbindelse egnet til anvendelse som et sprængstof, et mellemprodukt og fremgangsmåde til fremstilling af forbindelsen
DK1419255T3 (da) En ny gruppe af alfa-amylaser såvel som en fremgangsmåde til at identificere og udvinde nye alfa-amylaser.
ATE323759T1 (de) Rückgewinnung eines proteins bei hohem ph-wert
DK1422237T3 (da) Fremgangsmåder til rekombinant frembringelse af antifusogene peptider
DK1513952T3 (da) Sammensætninger og fremgangsmåder til frembringelse af nukleinsyre af sputum
ATE340857T1 (de) Verfahren zur herstellung von l-aminosäuren durch erhöhung des zellulären nadph
DK0751144T3 (da) Nye antracyclin-pro-drugs, fremgangsmåde til fremstilling deraf samt antitumorsammensætninger og et komponentkit indeholdende disse pro-drugs
WO2004052855A3 (en) Substituted 3-carbonyl-1h-indol-1-yl acetic acid derivatives as inhibitors of plasminogen activator inhibitor-1 (pai-1)
BRPI0410861A (pt) métodos e composições para a produção de aminoácidos
DK1077218T3 (da) Peptidomimetiske forbindelser omfattende RGD-sekvenser, som er nyttige som integrininhibitorer
CY1108804T1 (el) Μεθοδος παρασκευης της 4β-αμινο-4'-δεμεθυλ-4- δεσοξυποδοφυλλοτοξινης.
ATE420161T1 (de) Monopropylenglykol als fermentationszusatz
PL1654284T3 (pl) Sposób uzyskiwania roztworu alfa-1-antytrypsyny
EP1420028A3 (fr) Pocédé de synthèse du perindopril et de ses sels pharmaceutiquement acceptables
NO20002600D0 (no) Mellomprodukter og fremgangsmÕter for anvendelse ved semisyntesen av paclitaxel og analoger
DK1395661T3 (da) Fremgangsmåde til fremstilling af D-pantothensyre og/eller salte deraf
Kim et al. Production of $\alpha $-Glucosidase Inhibitor by $\beta $-Glucosidase Inhibitor-Producing Bacillus lentimorbus B-6
BR0005798A (pt) Processo para preparação fermentativa del-aminoácidos usando bactérias corineforme
DK1417301T3 (da) Carbonhydrater og polyoler til oplösning af proteinkrystaller
DE60120379D1 (de) HYDROPEROXIDLYASE der WARZENMELONE (CUCUMIS MELO) SOWIE DErEN VERWENDUNGEN
BR0309526B1 (pt) “Metiltiofenocarboxanilidas, processo para sua preparação e sua aplicação, composição para combater microorganismos indesejados compreendendo as referidas metiltiofenocarboxanilidas e seu processo de preparação, bem como processo para combater microorganismos indesejados ”
LU90992I2 (fr) Xigris-drotrécogine alfa(activée) et ses dérivés hpharmaceutiquement acceptables
DK0710290T3 (da) Fremgangsmåde til præcipitering af naturlige avermectiner og fermentativ fremstilling af samme