DK2801363T3 - Fremgangsmåde til opbevaring af sorterede spermatozoer - Google Patents
Fremgangsmåde til opbevaring af sorterede spermatozoer Download PDFInfo
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- DK2801363T3 DK2801363T3 DK14002489.4T DK14002489T DK2801363T3 DK 2801363 T3 DK2801363 T3 DK 2801363T3 DK 14002489 T DK14002489 T DK 14002489T DK 2801363 T3 DK2801363 T3 DK 2801363T3
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- sperm
- dispersion
- cells
- spermatozoa
- mammal
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Classifications
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
- A01N1/12—Chemical aspects of preservation
- A01N1/122—Preservation or perfusion media
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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- A01N1/122—Preservation or perfusion media
- A01N1/126—Physiologically active agents, e.g. antioxidants or nutrients
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- A—HUMAN NECESSITIES
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
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- C—CHEMISTRY; METALLURGY
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C12N5/061—Sperm cells, spermatogonia
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Claims (12)
1. Fremgangsmåde til opbevaring af sorterede spermatozoer, hvilken fremgangsmåde omfatter a. at danne en sæddispersion, hvilken sæddispersion omfatter spermatozoer og en motilitetshæmmer, således at spermatozoerne har midlertidigt induceret immotilitet i forhold til endogene ejakulerede spermatozoer, hvor sæddispersionen omfatter et DNA-selektivt farvestof, b. at sortere sæddispersionen i separate populationer, hvor spermatozoerne i én af populationerne omfatter mindst 65% X-kromosombærende sædceller eller mindst 65% Y-kromosombærende sædceller, c. at opbevare denne ene population ved en temperatur på - 196°C til -4°C i mindst 24 timer med tilsætning af et kryobeskyttelsesmiddel, som omfatter en motilitetshæmmer, for at minimere indvirkningen på levedygtighed eller på motilitet efter optøning som følge af nedkøling eller frysning.
2. Fremgangsmåde ifølge krav 1, som endvidere omfatter følgende trin: d. at føre de immotile spermatozoer tilbage til en aktiv tilstand ved at separere cellerne fra motilitetshæmmeren eller ved at eksponere dem for luft, og eventuelt ved at fortynde cellerne i en fysiologisk saltvandsopløsning eller en buffer, såsom TCA-buffer eller PBS, hvor banehastigheden, progressionshastigheden eller begge hastigheder for mindst 20% af de genaktiverede celler er mindst 50% af banehastigheden, progressionshastigheden eller begge hastigheder for sædceller fra det friske ejakulat som målt ved HTM-IVOS-sædanalyse.
3. Fremgangsmåde ifølge krav 1 eller 2, hvor koncentrationen af spermatozoer i sæddispersionen er mindst 0,04 x 106 sæd/ml.
4. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor motilitetshæmmeren omfatter kalium og eventuelt natrium, idet det molære forhold mellem henholdsvis kalium og natrium er større end 1:1.
5. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor sæddispersionen omfatter en kilde til carbonat.
6. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor dispersionen omfatter NaHCCb, KHCO3 og C6H807‘H20.
7. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor sæddispersionen endvidere omfatter et kryokonserveringsmiddel.
8. Fremgangsmåde ifølge krav 6, hvor kryokonserveringsmidlet omfatter glycerol og/eller æggeblomme.
9. Fremgangsmåde ifølge et hvilket som helst af kravene 6 eller 7, hvor kryokonserveringsmidlet omfatter glycerol, TRIS, citronsyre og fructose.
10. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor spermatozoerne i sæddispersionen stammer fra mere end ét pattedyr fra samme art.
11. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor spermatozoerne i sæddispersionen stammer fra et pattedyr valgt fra gruppen bestående af pattedyr af oksefamilien, pattedyr af svinefamilien, pattedyr af hestefamilien og svin.
12. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, hvor sæddispersionen opbevares i mindst 24 timer inden inseminering af et hunpattedyr med sæddispersionen.
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Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2264427B1 (en) | 1997-01-31 | 2017-05-03 | Xy, Llc | Optical apparatus with focussing reflector for converging radiation onto a flow of particles, and related method of analysis |
| US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
| US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| PT1917974E (pt) | 1998-07-30 | 2011-02-22 | Xy Llc | Sistema de inseminação artificial não cirúrgica em equinos |
| US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
| IL152714A (en) | 2000-05-09 | 2014-03-31 | Xy Llc | High-purity spermatozoa populations carrying chromosome-x and chromosome-y |
| US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
| WO2002043486A1 (en) | 2000-11-29 | 2002-06-06 | Xy, Inc. | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
| US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
| JP4595067B2 (ja) | 2002-08-01 | 2010-12-08 | エックスワイ，エルエルシー | 低圧精子細胞分離システム |
| CA2534394C (en) | 2002-08-15 | 2013-01-08 | Xy, Inc. | High resolution flow cytometer |
| US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| BRPI0408857B1 (pt) | 2003-03-28 | 2018-09-11 | Inguran Llc | aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo |
| ES2541121T3 (es) | 2003-05-15 | 2015-07-16 | Xy, Llc | Clasificación eficiente de células haploides por sistemas de citometría de flujo |
| DK2801363T3 (da) | 2004-03-29 | 2018-05-28 | Inguran Llc | Fremgangsmåde til opbevaring af sorterede spermatozoer |
| CA2574499C (en) | 2004-07-22 | 2016-11-29 | Monsanto Technology Llc | Process for enriching a population of sperm cells |
| DE102005044530B4 (de) * | 2004-09-16 | 2010-02-18 | Masterrind Gmbh | Verfahren zur geschlechtsspezifischen Selektion von Säugerspermatozoen |
| US7618770B2 (en) | 2005-07-29 | 2009-11-17 | Xy, Inc. | Methods and apparatus for reducing protein content in sperm cell extenders |
| US9433484B2 (en) * | 2007-07-27 | 2016-09-06 | Brad K. Stroud | Artificial breeding techniques for bovines including semen diluents and AI apparatus |
| US8415094B2 (en) * | 2007-12-21 | 2013-04-09 | Jaffar Ali bin M. Abdullah | Protein-free gamete and embryo handling and culture media products |
| CN101554152B (zh) * | 2009-05-21 | 2012-05-09 | 西北农林科技大学 | 一种冷冻精液稀释液及其配制方法 |
| CA2781736A1 (en) * | 2009-11-24 | 2011-06-03 | Biassex Pty Ltd. | Method for influencing sex selection in artificial insemination and apparatus for same |
| US11622844B2 (en) | 2010-08-10 | 2023-04-11 | Maximate, Llc | Method, apparatus and kit for artificial insemination of bovine |
| JP5689968B2 (ja) | 2010-08-10 | 2015-03-25 | ストラウド，ブラツド・ケイ | 畜牛の人工授精において使用される精子の数を減少させるための方法および装置 |
| US10610343B2 (en) | 2013-07-03 | 2020-04-07 | Brad K. Stroud | Method, apparatus and kit for artificial insemination of bovine |
| CN103249829B (zh) | 2010-08-20 | 2015-08-19 | 孙钰 | 用于自动化精子操作的系统和方法 |
| WO2012167151A1 (en) | 2011-06-01 | 2012-12-06 | Inguran, Llc | Compositions and methods for improving the quality of processed sperm |
| US9781919B2 (en) | 2011-06-01 | 2017-10-10 | Inguran, Llc | Compositions and methods for improving the quality of processed sperm |
| US12595500B2 (en) | 2011-09-26 | 2026-04-07 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| CN107058059B (zh) | 2011-09-26 | 2020-08-07 | 基因技术股份公司 | 高效的小体积核酸合成 |
| WO2014153188A2 (en) | 2013-03-14 | 2014-09-25 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| AU2013232381B2 (en) | 2012-03-14 | 2018-08-30 | Membrane Protective Technologies, Inc. | System and substances for cryopreservation of viable cells |
| WO2013152220A2 (en) | 2012-04-04 | 2013-10-10 | Life Technologies Corporation | Tal-effector assembly platform, customized services, kits and assays |
| US9888990B2 (en) | 2012-06-06 | 2018-02-13 | Inguran, Llc | Methods for use of sex sorted semen to improve genetic management in swine |
| US9433195B2 (en) | 2012-06-06 | 2016-09-06 | Inguran, Llc | Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells |
| US9034161B2 (en) | 2012-08-23 | 2015-05-19 | Douglas T. Carrell | Sperm separation devices and associated methods |
| CA2886782C (en) | 2012-10-05 | 2020-03-10 | Inguran, Llc | High efficiency methods of sex sorting sperm |
| US10620213B2 (en) | 2012-10-05 | 2020-04-14 | Inguran, Llc | High pressure sperm sorting and flow cytometer methods |
| WO2016011174A1 (en) | 2014-07-15 | 2016-01-21 | Life Technologies Corporation | Compositions and methods for nucleic acid assembly |
| EP3209766A4 (en) * | 2014-10-20 | 2018-08-15 | University of Utah Research Foundation | Tissue sample processing system and associated methods |
| WO2016081864A1 (en) | 2014-11-20 | 2016-05-26 | Inguran, Llc | Low sugar sperm media and compositions |
| US10407676B2 (en) | 2014-12-09 | 2019-09-10 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| US20180195099A1 (en) | 2015-07-07 | 2018-07-12 | Thermo Fisher Scientific Geneart Gmbh | Enzymatic synthesis of nucleic acid sequences |
| US20180291413A1 (en) | 2015-10-06 | 2018-10-11 | Thermo Fisher Scientific Geneart Gmbh | Devices and methods for producing nucleic acids and proteins |
| PT110231B (pt) * | 2017-08-02 | 2021-02-22 | Universidade Do Porto | Potenciadores de mtor e suas utilizações para melhorar a qualidade e função do esperma durante o armazenamento |
| EP3814494B1 (en) | 2018-06-29 | 2023-11-01 | Thermo Fisher Scientific GENEART GmbH | High throughput assembly of nucleic acid molecules |
| CN113226204A (zh) * | 2018-11-02 | 2021-08-06 | 安卓威尔生命科学有限责任公司 | 通过确定精子获能鉴定男性能育性的状态和伴侣收集试剂盒 |
| US10603075B1 (en) | 2018-11-30 | 2020-03-31 | Ohana Biosciences, Inc. | Compositions and methods for enhancing sperm function |
| GB201905303D0 (en) | 2019-04-15 | 2019-05-29 | Thermo Fisher Scient Geneart Gmbh | Multiplex assembly of nucleic acid molecules |
| EP4114972A1 (en) | 2020-03-06 | 2023-01-11 | Life Technologies Corporation | High sequence fidelity nucleic acid synthesis and assembly |
| DE102021002257A1 (de) | 2021-04-29 | 2022-11-03 | Forschungsverbund Berlin E.V. | Mittel zur Konservierung von Säugetiersperma und Verwendung des Mittels |
| EP4638776A1 (en) | 2022-12-19 | 2025-10-29 | Thermo Fisher Scientific GENEART GmbH | Retrieval of sequence-verified nucleic acid molecules |
Family Cites Families (634)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3005756A (en) * | 1958-11-14 | 1961-10-24 | Noland L Van Demark | Diluter containing carbon dioxide for preserving semen |
| US3005759A (en) * | 1959-04-24 | 1961-10-24 | American Zinc Inst | Zinc electroplating |
| US3299354A (en) | 1962-07-05 | 1967-01-17 | Coulter Electronics | Aperture tube structure for particle study apparatus |
| US3499435A (en) | 1967-06-02 | 1970-03-10 | Paul E Rockwell | Esophageal probe for use in monitoring |
| US3547526A (en) | 1967-10-26 | 1970-12-15 | Kollsman Instr Corp | Optical beam cross-section converter |
| US3810010A (en) | 1968-11-02 | 1974-05-07 | Telefunken Patent | Particle analysis method and apparatus wherein liquid containing particles is sucked into a constricted flow path |
| US3829216A (en) | 1968-11-26 | 1974-08-13 | M Persidsky | Optical system and method for counting sperm cells |
| DE1815352C3 (de) | 1968-12-18 | 1975-03-20 | Wolfgang Prof. Dr. Dittrich | Automatisches MeB- und Zählgerät für die Teilchen einer Dispersion |
| US3894529A (en) | 1969-04-10 | 1975-07-15 | Bio Controls Inc | Method and means for controlling the sex of mammalian offspring and product therefor |
| US4474875A (en) | 1969-04-10 | 1984-10-02 | Wallace Shrimpton | Method and means for controlling the sex of mammalian offspring and product therefor |
| US4327177A (en) | 1969-04-10 | 1982-04-27 | Wallace Shrimpton | Method and means for controlling the sex of mammalian offspring and product therefor |
| DE1919628C3 (de) | 1969-04-18 | 1975-04-10 | Wolfgang Prof. Dr. Dittrich | Anordnung zum automatischen Zählen und/oder Klassifizieren von in einem strömungsfähigen Medium dispergierten Teilchen |
| US3687806A (en) | 1969-11-04 | 1972-08-29 | Bio Controls Inc | Method for controlling sex of mammalian offspring |
| US3788744A (en) | 1970-01-14 | 1974-01-29 | Bio Physics Systems Inc | Method and apparatus for photoanalysis |
| US3661460A (en) | 1970-08-28 | 1972-05-09 | Technicon Instr | Method and apparatus for optical analysis of the contents of a sheathed stream |
| US3816249A (en) | 1970-11-23 | 1974-06-11 | B Bhattacharya | Universal medium and method for extending the useful life of semen in vitro |
| US3644128A (en) | 1970-12-28 | 1972-02-22 | Stuart Lipner | Method of preparing comminuted meat products |
| US3756459A (en) | 1971-01-12 | 1973-09-04 | Damon Corp | Method and apparatus for metering fluid utilizing pressure differentials |
| US3791384A (en) | 1971-07-15 | 1974-02-12 | Schaumann H | Artificial insemination of sows |
| US3833796A (en) | 1971-10-13 | 1974-09-03 | Georgia Tech Res Inst | Method and apparatus for chromosome digitizing |
| BE793185A (fr) | 1971-12-23 | 1973-04-16 | Atomic Energy Commission | Appareil pour analyser et trier rapidement des particules telles que des cellules biologiques |
| US3826364A (en) | 1972-05-22 | 1974-07-30 | Univ Leland Stanford Junior | Particle sorting method and apparatus |
| US3761941A (en) | 1972-10-13 | 1973-09-25 | Mead Corp | Phase control for a drop generating and charging system |
| CA1029833A (en) | 1973-02-23 | 1978-04-18 | Hildegarde Goehde | Apparatus for the automatic counting and measuring of suspended particles |
| US3791517A (en) | 1973-03-05 | 1974-02-12 | Bio Physics Systems Inc | Digital fluidic amplifier particle sorter |
| US4009260A (en) | 1973-04-19 | 1977-02-22 | Schering Aktiengesellschaft | Fractionation of sperm |
| USRE29141E (en) | 1973-06-14 | 1977-02-22 | Coulter Electronics, Inc. | Apparatus for orienting generally flat particles for sensing |
| US3893766A (en) | 1973-06-14 | 1975-07-08 | Coulter Electronics | Apparatus for orienting generally flat particles for slit-scan photometry |
| US3947093A (en) | 1973-06-28 | 1976-03-30 | Canon Kabushiki Kaisha | Optical device for producing a minute light beam |
| US3944917A (en) | 1973-08-13 | 1976-03-16 | Coulter Electronics, Inc. | Electrical sensing circuitry for particle analyzing device |
| US3909744A (en) | 1973-09-24 | 1975-09-30 | United Technologies Corp | Unstable resonator system producing a high irradiance beam in the far field |
| US3906929A (en) | 1973-11-23 | 1975-09-23 | Lynn Lawrence Augspurger | Processes for reproduction of cellular bodies |
| FR2254639B1 (da) | 1973-12-18 | 1978-06-02 | Agronomique Inst Nat Rech | |
| US4070617A (en) | 1974-05-08 | 1978-01-24 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Device for controlling the particle flow in an apparatus for measuring the properties of particles suspended in liquid |
| US3973196A (en) | 1974-05-24 | 1976-08-03 | Coulter Electronics, Inc. | Method and apparatus for ejecting a metered amount of particulate sample |
| US3963606A (en) | 1974-06-03 | 1976-06-15 | Coulter Electronics, Inc. | Semi-automatic adjusting delay for an electronic particle separator |
| US3877430A (en) | 1974-07-17 | 1975-04-15 | Horst K Wieder | Artificial insemination apparatus |
| US4083957A (en) | 1974-07-26 | 1978-04-11 | Lang John L | Process for the alteration of the sex-ratio of mammals |
| US4006360A (en) | 1974-08-21 | 1977-02-01 | Block Engineering, Inc. | Method of discriminating between dyed particles and background fluorescence of the dye |
| US4092229A (en) | 1975-12-17 | 1978-05-30 | Bhattacharya Bhairab C | Thermal convection counter streaming sedimentation and forced convection galvanization method for controlling the sex of mammalian offspring |
| US3976197A (en) | 1974-11-22 | 1976-08-24 | Bhattacharya Bhairab C | Thermal convection counter streaming sedimentation method and apparatus for controlling the sex of mammalian offspring |
| US4014611A (en) | 1975-04-30 | 1977-03-29 | Coulter Electronics, Inc. | Aperture module for use in particle testing apparatus |
| DE2521236C3 (de) | 1975-05-10 | 1978-12-14 | Hildegard Dr. 4400 Muenster Goehde Geb. Kuhl | Einrichtung zum Zählen und Messen von in einer Flüssigkeit suspendierten Teilchen |
| US3960449A (en) | 1975-06-05 | 1976-06-01 | The Board Of Trustees Of Leland Stanford Junior University | Measurement of angular dependence of scattered light in a flowing stream |
| US4058732A (en) | 1975-06-30 | 1977-11-15 | Analytical Radiation Corporation | Method and apparatus for improved analytical fluorescent spectroscopy |
| US4007087A (en) | 1975-10-17 | 1977-02-08 | Gametrics Limited | Sperm fractionation and storage |
| AU2154077A (en) | 1976-01-27 | 1978-07-27 | Univ Edinburgh | Control of sex ratio in mammalian offspring |
| US4302166A (en) | 1976-04-22 | 1981-11-24 | Coulter Electronics, Inc. | Droplet forming apparatus for use in producing uniform particles |
| US4162282A (en) | 1976-04-22 | 1979-07-24 | Coulter Electronics, Inc. | Method for producing uniform particles |
| GB1563856A (en) | 1976-06-10 | 1980-04-02 | Coulter Electronics | Methods and apparatus for delectively separating small particles suspended in a liquid |
| GB1583150A (en) | 1976-08-02 | 1981-01-21 | Milk Marketing Board | Apparatus for collecting eggs |
| US4110604A (en) | 1976-11-04 | 1978-08-29 | Becton, Dickinson And Company | Particle density measuring system |
| DE2709399C3 (de) | 1977-03-04 | 1980-07-24 | Goehde, Wolfgang, Dr., 4400 Muenster | Einrichtung zum Messen von Zelleigenschaften |
| DE2716095C2 (de) | 1977-04-12 | 1987-02-19 | Becton, Dickinson and Co., Paramus, N.J. | Verfahren zur Steuerung der Bewegungsbahn von in einem Elektrolyten suspendierten Teilchen und Vorrichtung zur Durchführung dieses Verfahrens |
| US4448767A (en) | 1977-10-11 | 1984-05-15 | Sumar Corporation | Preparation of monospecific male-specific antibody and the use thereof for increasing the percentage of mammalian offspring of either sex |
| US4191749A (en) | 1977-10-11 | 1980-03-04 | Bryant Bernard J | Method and material for increasing the percentage of mammalian offspring of either sex |
| US4189236A (en) | 1978-03-20 | 1980-02-19 | Coulter Electronics, Inc. | Ellipsoid-conic radiation collector and method |
| US4179218A (en) | 1978-05-15 | 1979-12-18 | The Boeing Company | Particle size analyzer |
| US4251733A (en) | 1978-06-29 | 1981-02-17 | Hirleman Jr Edwin D | Technique for simultaneous particle size and velocity measurement |
| DE2832091A1 (de) | 1978-07-21 | 1980-01-31 | Eidenschink Henning | Optisches verfahren zur bestimmung der teilchengroesse kolloidaler loesungen und messgeraet zur durchfuehrung des verfahrens |
| US4225405A (en) | 1978-08-16 | 1980-09-30 | Lawson Rommom L | Process for separation and collection of viable female and male spermatozoa |
| US4276139A (en) | 1978-08-16 | 1981-06-30 | Lawson Rommon L | Process for magnetic separation and collection of viable female and male spermatozoa |
| US4230558A (en) | 1978-10-02 | 1980-10-28 | Coulter Electronics, Inc. | Single drop separator |
| US4341471A (en) | 1979-01-02 | 1982-07-27 | Coulter Electronics, Inc. | Apparatus and method for measuring the distribution of radiant energy produced in particle investigating systems |
| US4267268A (en) | 1979-03-12 | 1981-05-12 | Nelson Jr Robert A | Spermatozoa extenders |
| US4274408A (en) | 1979-03-26 | 1981-06-23 | Beatrice Nimrod | Method for guide-wire placement and novel syringe therefor |
| US4200802A (en) | 1979-03-28 | 1980-04-29 | The United States Of America As Represented By The United States Department Of Energy | Parabolic cell analyzer |
| NO144002C (no) | 1979-04-10 | 1981-05-27 | Norsk Hydro S Inst For Kreftfo | Anordning for anvendelse ved vaeskestroemsfotometri |
| US4408877A (en) | 1979-04-10 | 1983-10-11 | Ernst Leitz Wetzlar Gmbh | Device for hydrodynamic focussing of a particle-suspension in a liquid flow cytophotometer |
| US4263508A (en) | 1979-04-20 | 1981-04-21 | Research Corporation | Pulse edge measurement for determining particle dimensional characteristics |
| US4255021A (en) | 1979-04-20 | 1981-03-10 | The United States Of America As Represented By The United States Department Of Energy | Optical device with conical input and output prism faces |
| US4318480A (en) | 1979-08-20 | 1982-03-09 | Ortho Diagnostics, Inc. | Method and apparatus for positioning the point of droplet formation in the jetting fluid of an electrostatic sorting device |
| US4318481A (en) | 1979-08-20 | 1982-03-09 | Ortho Diagnostics, Inc. | Method for automatically setting the correct phase of the charge pulses in an electrostatic flow sorter |
| US4317520A (en) | 1979-08-20 | 1982-03-02 | Ortho Diagnostics, Inc. | Servo system to control the spatial position of droplet formation of a fluid jet in a cell sorting apparatus |
| US4325483A (en) | 1979-08-20 | 1982-04-20 | Ortho Diagnostics, Inc. | Method for detecting and controlling flow rates of the droplet forming stream of an electrostatic particle sorting apparatus |
| US4318482A (en) | 1979-08-20 | 1982-03-09 | Ortho Diagnostics, Inc. | Method for measuring the velocity of a perturbed jetting fluid in an electrostatic particle sorting system |
| DE2943116C2 (de) | 1979-10-25 | 1986-06-19 | Gesellschaft für Strahlen- und Umweltforschung mbH, 8000 München | Einrichtung zur durchflußcytometrischen Reaktions- und/oder Diffusionsmessung |
| US4284355A (en) | 1979-10-29 | 1981-08-18 | Ortho Diagnostics, Inc. | Automated method for cell volume determination |
| US4400764A (en) | 1980-02-05 | 1983-08-23 | The Boeing Company | Low backscatter illumination system |
| US4367043A (en) | 1980-05-05 | 1983-01-04 | Leland Stanford Junior University | Method and means for delivering liquid samples to a sample scanning device |
| US4362246A (en) | 1980-07-14 | 1982-12-07 | Adair Edwin Lloyd | Method of treating collected mammal semen and separating sperm into X Y components |
| US4348107A (en) | 1980-07-18 | 1982-09-07 | Coulter Electronics, Inc. | Orifice inside optical element |
| EP0046345A3 (en) | 1980-08-15 | 1982-03-03 | Ortho Diagnostic Systems Inc. | Controlled hydrodynamic flow in flow cytometry systems |
| US4350410A (en) | 1980-10-08 | 1982-09-21 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Multiprism collimator |
| US4691829A (en) | 1980-11-03 | 1987-09-08 | Coulter Corporation | Method of and apparatus for detecting change in the breakoff point in a droplet generation system |
| US4487320A (en) | 1980-11-03 | 1984-12-11 | Coulter Corporation | Method of and apparatus for detecting change in the breakoff point in a droplet generation system |
| US4395676A (en) | 1980-11-24 | 1983-07-26 | Coulter Electronics, Inc. | Focused aperture module |
| US4361400A (en) | 1980-11-26 | 1982-11-30 | The United States Of America As Represented By The United States Department Of Energy | Fluidic assembly for an ultra-high-speed chromosome flow sorter |
| US4680258A (en) | 1981-03-24 | 1987-07-14 | Sloan-Kettering Institute For Cancer Research | Process for sex determination in man by use of monoclonal antibodies to the H-Y antigen |
| US4818103A (en) | 1981-05-15 | 1989-04-04 | Ratcom | Flow cytometry |
| US4673288A (en) | 1981-05-15 | 1987-06-16 | Ratcom, Inc. | Flow cytometry |
| EP0068404B1 (en) | 1981-06-24 | 1985-10-02 | Becton Dickinson and Company | Analyzer for simultaneously determining volume and light emission characteristics of particles |
| US4339434A (en) | 1981-08-17 | 1982-07-13 | Gametrics Limited | Method of increasing the incidence of female offspring |
| US4395397A (en) | 1981-09-17 | 1983-07-26 | Sidney Farber Cancer Institute, Inc. | Apparatus and method for killing unwanted cells |
| US4422761A (en) | 1981-09-28 | 1983-12-27 | Frommer Joseph C | Photo-electric particle sensing system |
| US4515274A (en) | 1981-12-02 | 1985-05-07 | Coulter Corporation | Particle analyzing and sorting apparatus |
| JPS59500340A (ja) | 1982-03-08 | 1984-03-01 | モトロ−ラ・インコ−ポレ−テツド | 集積回路のリ−ドフレ−ム |
| US4511661A (en) | 1982-03-19 | 1985-04-16 | University Patents, Inc. | ATCC HB8116 And its monoclonal anti-H-Y antibody, Hyclonalan |
| US4498766A (en) | 1982-03-25 | 1985-02-12 | Becton, Dickinson And Company | Light beam focal spot elongation in flow cytometry devices |
| DE3315194A1 (de) | 1982-04-29 | 1983-11-03 | International Remote Imaging Systems Inc., 91311 Chatsworth, Calif. | Verfahren zum trennen von in einer fluidprobe stroemenden teilchen |
| DE3315195A1 (de) | 1982-04-29 | 1983-11-03 | International Remote Imaging Systems Inc., 91311 Chatsworth, Calif. | Verfahren zum ausrichten von teilchen in einer fluidprobe |
| US4629687A (en) | 1982-07-29 | 1986-12-16 | Board Of Trustees Of Michigan State University | Positive selection sorting of cells |
| GB2125181B (en) | 1982-08-11 | 1986-01-29 | Coulter Electronics | Flow cells for particle study |
| US4559309A (en) | 1982-09-01 | 1985-12-17 | Memorial Sloan Kettering Cancer Center | Flow cytometry-fluorescence measurements for characterizing sperm |
| EP0120854A4 (en) | 1982-10-05 | 1985-06-10 | Genetic Engineering Inc | PROCESS FOR TREATING SPERM RECOVERED FROM A MAMMAL AND SEPARATING THE SPERM INTO COMPONENTS X AND Y. |
| US4501366A (en) | 1982-12-14 | 1985-02-26 | Adolph Coors Company | Photomultiplier tube assembly |
| DE3372137D1 (en) | 1982-12-21 | 1987-07-23 | Crosfield Electronics Ltd | Light beam-splitter |
| US4492436A (en) | 1983-01-03 | 1985-01-08 | At&T Bell Laboratories | Polarization independent beam splitter |
| JPS59143146A (ja) | 1983-02-07 | 1984-08-16 | Nippon Kogaku Kk <Nikon> | ミラ−集光型照明光学系 |
| CA1206559A (en) | 1983-03-04 | 1986-06-24 | Robert E. Auer | Method of and apparatus for detecting change in the breakoff point of a droplet generation system |
| IT1197570B (it) | 1983-02-11 | 1988-12-06 | Serono Ist Farm | Miscele di fsh e lh da ipofisi porcine in rapporto definito |
| CH651930A5 (en) | 1983-03-24 | 1985-10-15 | Coulter Corp | Apparatus for analysis and sorting of particles |
| JPS59174742A (ja) | 1983-03-25 | 1984-10-03 | Agency Of Ind Science & Technol | 微小粒子を区分又は選別する方法及びその装置 |
| GB2145112B (en) | 1983-04-27 | 1987-02-18 | Milk Marketing Board | Sorting living spermatozoa |
| US4523809A (en) | 1983-08-04 | 1985-06-18 | The United States Of America As Represented By The Secretary Of The Air Force | Method and apparatus for generating a structured light beam array |
| NZ207393A (en) | 1983-08-05 | 1987-03-31 | Neal Lloyd First | Staining dna in living cells |
| US4538733A (en) | 1983-10-14 | 1985-09-03 | Becton, Dickinson And Company | Particle sorter with neutralized collection wells and method of using same |
| US4585736A (en) | 1983-10-18 | 1986-04-29 | The United States Of America As Represented By The United States Department Of Energy | Flow cytometric measurement of total DNA and incorporated halodeoxyuridine |
| US4780406A (en) | 1983-10-18 | 1988-10-25 | The Regents Of The University Of California | Flow cytometric measurement of total DNA and incorporated halodeoxyuridine |
| US4735504A (en) | 1983-10-31 | 1988-04-05 | Technicon Instruments Corporation | Method and apparatus for determining the volume & index of refraction of particles |
| ATE58017T1 (de) | 1983-12-24 | 1990-11-15 | Inotech Ag | Vorrichtung zum fuehren und sammeln von licht in der fotometrie od. dgl. |
| US4573796A (en) | 1984-01-06 | 1986-03-04 | The United States Of America As Represented By The United States Department Of Energy | Apparatus for eliminating background interference in fluorescence measurements |
| US4631483A (en) | 1984-02-01 | 1986-12-23 | Coulter Electronics, Inc. | Particle analyzing apparatus and method of moving particles in suspension through such apparatus |
| US4965204A (en) | 1984-02-06 | 1990-10-23 | The Johns Hopkins University | Human stem cells and monoclonal antibodies |
| US4714680B1 (en) | 1984-02-06 | 1995-06-27 | Univ Johns Hopkins | Human stem cells |
| EP0172242A1 (en) | 1984-02-29 | 1986-02-26 | Research Corporation | Flow cytometers |
| US4545677A (en) | 1984-03-05 | 1985-10-08 | Becton, Dickinson And Company | Prismatic beam expander for light beam shaping in a flow cytometry apparatus |
| US4600302A (en) | 1984-03-26 | 1986-07-15 | Becton, Dickinson And Company | Flow cytometry apparatus with uniform incoherent light excitation |
| DE3412620A1 (de) | 1984-04-04 | 1985-10-17 | Basf Ag, 6700 Ludwigshafen | Laseroptische anordnung zur messung des dispergiergrades in stroemenden systemen |
| US4609286A (en) | 1984-04-16 | 1986-09-02 | Becton, Dickinson And Company | Dispersion prism for separation of wavelengths of spectrally rich light in a flow cytometry apparatus |
| US4605558A (en) | 1984-04-20 | 1986-08-12 | Wallace Shrimpton | Process for cell separation |
| US4660971A (en) | 1984-05-03 | 1987-04-28 | Becton, Dickinson And Company | Optical features of flow cytometry apparatus |
| FR2563726B1 (fr) | 1984-05-04 | 1986-10-10 | Robert Cassou | Appareil d'insemination artificielle, notamment des carnivores |
| FR2566543B1 (fr) | 1984-06-20 | 1988-02-26 | Commissariat Energie Atomique | Dispositif optique a rendement de collection eleve et cytofluorimetre en faisant application |
| EP0171676B1 (en) | 1984-07-31 | 1990-11-07 | Hitachi, Ltd. | Free-flow electrophoretic separation method and apparatus therefor |
| US4661913A (en) | 1984-09-11 | 1987-04-28 | Becton, Dickinson And Company | Apparatus and method for the detection and classification of articles using flow cytometry techniques |
| EP0177718B1 (de) | 1984-09-11 | 1989-12-06 | Partec AG | Verfahren und Vorrichtung zur Sortierung von mikroskopischen Partikeln |
| US4598408A (en) | 1984-10-22 | 1986-07-01 | Trw Inc. | High extraction efficiency cylindrical ring resonator |
| JPS61139747A (ja) | 1984-12-12 | 1986-06-27 | Canon Inc | 粒子解析装置 |
| JPS61159135A (ja) | 1984-12-31 | 1986-07-18 | Canon Inc | 粒子解析装置 |
| US4702598A (en) | 1985-02-25 | 1987-10-27 | Research Corporation | Flow cytometer |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| CA1250808A (en) | 1985-04-29 | 1989-03-07 | David W. Dresser | Semen sexing |
| US4662742A (en) | 1985-05-10 | 1987-05-05 | Becton, Dickinson And Company | Scatter/fluorescene beam splitter in a flow cytometry apparatus |
| US4877965A (en) | 1985-07-01 | 1989-10-31 | Diatron Corporation | Fluorometer |
| USRE34782E (en) | 1985-07-01 | 1994-11-08 | Diatron Corporation | Fluorometer |
| US4744090A (en) | 1985-07-08 | 1988-05-10 | Trw Inc. | High-extraction efficiency annular resonator |
| NO156916C (no) | 1985-07-10 | 1987-12-16 | Harald B Steen | Stroemningskammer for vaeskestroemsfotometer. |
| NO156917C (no) | 1985-07-16 | 1987-12-16 | Harald B Steen | Anordning for maaling av biologiske cellers lysspredning i vaeskestroemsfotometere. |
| US4989977A (en) | 1985-07-29 | 1991-02-05 | Becton, Dickinson And Company | Flow cytometry apparatus with improved light beam adjustment |
| US4794086A (en) | 1985-11-25 | 1988-12-27 | Liquid Air Corporation | Method for measurement of impurities in liquids |
| US4770992A (en) | 1985-11-27 | 1988-09-13 | Den Engh Gerrit J Van | Detection of specific DNA sequences by flow cytometry |
| US4999283A (en) | 1986-01-10 | 1991-03-12 | University Of Kentucky Research Foundation | Method for x and y spermatozoa separation |
| US5756696A (en) | 1986-01-16 | 1998-05-26 | Regents Of The University Of California | Compositions for chromosome-specific staining |
| US5447841A (en) | 1986-01-16 | 1995-09-05 | The Regents Of The Univ. Of California | Methods for chromosome-specific staining |
| US4710635A (en) | 1986-04-14 | 1987-12-01 | Becton, Dickinson And Company | Dual laser excitation from single laser source |
| NL8601000A (nl) | 1986-04-21 | 1987-11-16 | Jan Greve T H Twente Afdeling | Het gebruik van gepolariseerd licht in stromingscytometrie. |
| JPS62274238A (ja) | 1986-05-22 | 1987-11-28 | ベクトン・デイツキンソン・アンド・カンパニ− | フロ−サイトメトリ−装置に用いる光学的結合用ゲル |
| US4790653A (en) | 1986-05-22 | 1988-12-13 | Becton Dickinson And Company | Housing for a flow cytometry apparatus with particle unclogging feature |
| US4786165A (en) | 1986-07-10 | 1988-11-22 | Toa Medical Electronics Co., Ltd. | Flow cytometry and apparatus therefor |
| US4867908A (en) | 1986-08-29 | 1989-09-19 | Becton, Dickinson And Company | Method and materials for calibrating flow cytometers and other analysis instruments |
| US4704891A (en) | 1986-08-29 | 1987-11-10 | Becton, Dickinson And Company | Method and materials for calibrating flow cytometers and other analysis instruments |
| FR2609885B1 (fr) | 1987-01-22 | 1989-04-14 | Cassou Robert | Instrument pour l'insemination artificielle, le transfert d'embryons ou le prelevement de liquides folliculaires chez les mammiferes |
| US4780451B1 (en) | 1987-01-23 | 1995-04-04 | Asua International Inc | Composition and method for producing superovulation in cattle |
| US5162306A (en) | 1987-01-23 | 1992-11-10 | Donaldson Lloyd E | Composition and method for producing superovulation in mammals |
| ATE91789T1 (de) | 1987-02-17 | 1993-08-15 | Ratcom Inc | Durchflusszytometrie. |
| US4764013A (en) | 1987-03-23 | 1988-08-16 | The United States Of America As Represented By The United States Department Of Energy | Interferometric apparatus and method for detection and characterization of particles using light scattered therefrom |
| US4765737A (en) | 1987-03-30 | 1988-08-23 | Cornell Research Foundation | Cell size measurements using light in flow cytometry and cell sorting |
| US5346990A (en) | 1987-04-08 | 1994-09-13 | Cytogam, Inc. | Sex-associated membrane proteins and methods for increasing the probability that offspring will be of a desired sex |
| EP0286088B1 (en) | 1987-04-08 | 1994-09-14 | Hitachi, Ltd. | A sheath flow type flow-cell device |
| US5021244A (en) | 1988-12-06 | 1991-06-04 | Cytogam, Inc. | Sex-associated membrane antibodies and their use for increasing the probability that offspring will be of a desired sex |
| JPS63262565A (ja) | 1987-04-20 | 1988-10-28 | Hitachi Ltd | フロ−セル |
| EP0289677A3 (en) | 1987-04-27 | 1989-05-10 | Fritz K. Preikschat | Apparatus and method for particle analysis |
| EP0289200B2 (en) | 1987-04-27 | 1998-07-22 | Fritz K. Preikschat | Apparatus and method for particle analysis |
| FR2614626B1 (fr) | 1987-04-30 | 1989-07-21 | Ranoux Claude | Conteneur pour fecondation des ovocytes et replacement des embryons chez l'homme et l'animal |
| JP2642632B2 (ja) | 1987-07-03 | 1997-08-20 | 株式会社日立製作所 | 微粒子計測装置および微粒子計測方法 |
| GB8716285D0 (en) | 1987-07-10 | 1987-08-19 | Medical Res Council | Light collecting device |
| US4979093A (en) | 1987-07-16 | 1990-12-18 | Cavro Scientific Instruments | XYZ positioner |
| US4987539A (en) | 1987-08-05 | 1991-01-22 | Stanford University | Apparatus and method for multidimensional characterization of objects in real time |
| US4796788A (en) | 1987-08-26 | 1989-01-10 | Liqui-Box Corporation | Bag-in-box packaging and dispensing of substances which will not readily flow by gravity |
| US4758729A (en) | 1987-08-28 | 1988-07-19 | Spectra-Physics, Inc. | Apparatus and method for measuring the included angle of a reflective cone |
| DE3832901A1 (de) | 1987-10-02 | 1989-04-20 | Hitachi Ltd | Teilchenmessvorrichtung |
| US4793705A (en) | 1987-10-07 | 1988-12-27 | The United States Of America As Represented By The United States Department Of Energy | Single molecule tracking |
| US4831385A (en) | 1987-10-14 | 1989-05-16 | Burlington Industries, Inc. | Vacuum tray fluid-jet start-up system |
| US4980277A (en) | 1987-10-16 | 1990-12-25 | Cultor Ltd. | Cryoprotectant solution and method |
| US5712807A (en) | 1987-10-21 | 1998-01-27 | Bangham; James Andrew | Pulse analyzing method and apparatus |
| US5789155A (en) | 1987-10-30 | 1998-08-04 | California Institute Of Technology | Process for identifying nucleic acids and triple helices formed thereby |
| GB8726305D0 (en) | 1987-11-10 | 1987-12-16 | Secr Defence | Portable particle analysers |
| GB8726304D0 (en) | 1987-11-10 | 1987-12-16 | Secr Defence | Particle asymmetry analyser |
| US4845025A (en) | 1987-11-10 | 1989-07-04 | Coulter Corporation | Biological sample mixing apparatus and method |
| WO1989004470A1 (en) | 1987-11-10 | 1989-05-18 | The Secretary Of State For Defence In Her Britanni | Particle monitoring system |
| US5040890A (en) | 1987-11-25 | 1991-08-20 | Becton, Dickinson And Company | Sheathed particle flow controlled by differential pressure |
| US4988619A (en) | 1987-11-30 | 1991-01-29 | United States Department Of Energy | Flow cytometry apparatus |
| US4887721A (en) | 1987-11-30 | 1989-12-19 | The United States Of America As Represented By The United States Department Of Energy | Laser particle sorter |
| US4936465A (en) | 1987-12-07 | 1990-06-26 | Zoeld Tibor | Method and apparatus for fast, reliable, and environmentally safe dispensing of fluids, gases and individual particles of a suspension through pressure control at well defined parts of a closed flow-through system |
| US5219729A (en) | 1988-02-25 | 1993-06-15 | Serono Laboratories, Inc. | Fertility assay |
| US5622820A (en) | 1988-03-10 | 1997-04-22 | City Of Hope | Method for amplification and detection of RNA and DNA sequences |
| US4836038A (en) | 1988-03-18 | 1989-06-06 | Aim Instruments Ltd. | Automated sampler-injector apparatus and method for sampling a quantity of sample and testing portions of said quantity |
| US5057413A (en) | 1988-06-13 | 1991-10-15 | Becton, Dickinson And Company | Method for discriminating between intact and damaged cells in a sample |
| US5070080A (en) | 1988-08-10 | 1991-12-03 | Fahim Mostafa S | Method of inhibiting generation, maturation, motility and viability of sperm with minerals in bioavailable form |
| JPH0718785B2 (ja) | 1988-09-19 | 1995-03-06 | 株式会社日立製作所 | フローセル装置 |
| JP2635126B2 (ja) | 1988-09-30 | 1997-07-30 | 東亜医用電子株式会社 | 核の分葉指数を求めるための粒子分析装置及び方法 |
| JP2635125B2 (ja) | 1988-09-30 | 1997-07-30 | 東亜医用電子株式会社 | 核の分葉指数を求めるための粒子分析装置及び方法 |
| JPH02168160A (ja) | 1988-12-22 | 1990-06-28 | Omron Tateisi Electron Co | 細胞選別装置 |
| US5726009A (en) | 1989-03-20 | 1998-03-10 | Anticancer, Inc. | Native-state method and system for determining viability and proliferative capacity of tissues in vitro |
| JPH02289808A (ja) | 1989-04-28 | 1990-11-29 | Olympus Optical Co Ltd | 照明光学系 |
| US4981580A (en) | 1989-05-01 | 1991-01-01 | Coulter Corporation | Coincidence arbitration in a flow cytomery sorting system |
| CA2055494C (en) | 1989-05-10 | 2000-01-18 | Lawrence Arthur Johnson | Method to preselect the sex of offspring |
| US4942305A (en) | 1989-05-12 | 1990-07-17 | Pacific Scientific Company | Integrating sphere aerosol particle detector |
| DE68924275T2 (de) | 1989-05-12 | 1996-05-02 | Cytogam Inc | Mit dem geschlecht assoziierte membranproteine und methoden zur erhöhung der wahrscheinlichkeit, dass die nachkommenschaft das gewünschte geschlecht besitzt. |
| US5055393A (en) | 1989-06-13 | 1991-10-08 | Salk Institute Biotechnology/Industrial Associates, Inc. | Prenatal sex determination of bovine cells using male-specific oligonucleotides |
| US4954715A (en) | 1989-06-26 | 1990-09-04 | Zoeld Tibor | Method and apparatus for an optimized multiparameter flow-through particle and cell analyzer |
| JPH0353164A (ja) | 1989-07-20 | 1991-03-07 | Canon Inc | サンプル供給装置及びこれを用いたサンプル測定装置 |
| US5098657A (en) | 1989-08-07 | 1992-03-24 | Tsi Incorporated | Apparatus for measuring impurity concentrations in a liquid |
| US5030002A (en) | 1989-08-11 | 1991-07-09 | Becton, Dickinson And Company | Method and apparatus for sorting particles with a moving catcher tube |
| US5005981A (en) | 1989-09-08 | 1991-04-09 | Becton, Dickinson And Company | Apparatus for method for causing vortices in a test tube |
| US5215376A (en) | 1989-09-08 | 1993-06-01 | Becton, Dickinson And Company | Method for causing vortices in a test tube |
| DE69014507T2 (de) | 1989-09-13 | 1995-04-13 | Tiyoda Seisakusho Koushoku Kk | Gerät zur Zellenvorbehandlung für Durchfluss-Zytometrie. |
| JP2808321B2 (ja) | 1989-09-19 | 1998-10-08 | 東亜医用電子株式会社 | 細胞分析方法及び装置 |
| US5072382A (en) | 1989-10-02 | 1991-12-10 | Kamentsky Louis A | Methods and apparatus for measuring multiple optical properties of biological specimens |
| US5275787A (en) | 1989-10-04 | 1994-01-04 | Canon Kabushiki Kaisha | Apparatus for separating or measuring particles to be examined in a sample fluid |
| DE69025256T2 (de) | 1989-10-11 | 1996-06-27 | Canon Kk | Gerät und Verfahren zur Trennung von Teilchen aus flüssigkeitssuspendierten Teilchen in Zusammenhang mit deren Eigenschaften |
| JPH03140840A (ja) | 1989-10-26 | 1991-06-14 | Hitachi Ltd | 流動細胞分析装置 |
| FR2653885B1 (fr) | 1989-10-27 | 1994-01-14 | Abx | Appareil pour le comptage et la determination d'au moins une sous-population leucocytaire. |
| US5034613A (en) | 1989-11-14 | 1991-07-23 | Cornell Research Foundation, Inc. | Two-photon laser microscopy |
| US5101978A (en) | 1989-11-27 | 1992-04-07 | The United States Of America As Represented By The Secretary Of The Army | Fluidic sorting device for two or more materials suspended in a fluid |
| AU647741B2 (en) | 1989-12-01 | 1994-03-31 | Regents Of The University Of California, The | Methods and compositions for chromosome-specific staining |
| US5274240A (en) | 1990-01-12 | 1993-12-28 | The Regents Of The University Of California | Capillary array confocal fluorescence scanner and method |
| EP0448931B1 (en) | 1990-01-26 | 1996-04-03 | Canon Kabushiki Kaisha | Method for measuring a specimen by the use of fluorescence light |
| JP3049254B2 (ja) | 1990-02-08 | 2000-06-05 | シスメックス株式会社 | ２種類の光源を備えた光学式粒子分析装置 |
| IL93634A0 (en) | 1990-03-05 | 1990-12-23 | Galai Lab Ltd | Particle size analyzer |
| US5153117A (en) | 1990-03-27 | 1992-10-06 | Genetype A.G. | Fetal cell recovery method |
| US5492534A (en) | 1990-04-02 | 1996-02-20 | Pharmetrix Corporation | Controlled release portable pump |
| US5150313A (en) | 1990-04-12 | 1992-09-22 | Regents Of The University Of California | Parallel pulse processing and data acquisition for high speed, low error flow cytometry |
| US5076472A (en) | 1990-06-13 | 1991-12-31 | Becton, Dickinson And Company | Cleaning cycle for flow cytometers |
| US5087295A (en) | 1990-06-13 | 1992-02-11 | Becton Dickinson And Company | Cleaning cycle for flow cytometers |
| JPH0710226B2 (ja) | 1990-06-20 | 1995-02-08 | 株式会社ピーシーシーテクノロジー | プロトプラスト攪拌装置並びにそれを含む細胞融合装置及びフローサイトメーター装置 |
| JPH0716392B2 (ja) | 1990-06-20 | 1995-03-01 | 株式会社ピーシーシーテクノロジー | 細胞融合及び融合細胞選別システム |
| JPH04126065A (ja) | 1990-06-25 | 1992-04-27 | Saburo Okonogi | 食品素材の棒状体成型装置 |
| US5160974A (en) | 1990-06-25 | 1992-11-03 | Flow Science, Inc. | Closed sample cell for use in flow cytometry |
| US5559032A (en) | 1990-06-29 | 1996-09-24 | Pomeroy; Patrick C. | Method and apparatus for post-transfer assaying of material on solid support |
| JP3375957B2 (ja) | 1990-07-09 | 2003-02-10 | アムラド・コーポレイション・リミテッド | 白血病抑制因子を用いる胚の増強移植、増殖及び維持 |
| JP2939647B2 (ja) | 1990-07-24 | 1999-08-25 | シスメックス株式会社 | フローイメージングサイトメータにおける自動焦点調整方法 |
| IE76732B1 (en) | 1990-08-07 | 1997-11-05 | Becton Dickinson Co | One step test for absolute counts |
| US5259593A (en) | 1990-08-30 | 1993-11-09 | University Of Southern California | Apparatus for droplet stream manufacturing |
| JPH04115136A (ja) | 1990-09-05 | 1992-04-16 | Hitachi Ltd | 粒子計測装置 |
| US5132548A (en) | 1990-09-14 | 1992-07-21 | High Yield Technology | High sensitivity, large detection area particle sensor for vacuum applications |
| JP2957246B2 (ja) | 1990-09-14 | 1999-10-04 | 大和化成株式会社 | 微生物起源カルボキシペプチダーゼｂ様酵素 |
| JPH04126064A (ja) | 1990-09-14 | 1992-04-27 | Nitto Shokai:Kk | 二重包餡食品の製造法 |
| JPH04126080A (ja) | 1990-09-14 | 1992-04-27 | Juzo Udaka | 耐熱性キシロースイソメラーゼの製造方法 |
| US5204884A (en) | 1991-03-18 | 1993-04-20 | University Of Rochester | System for high-speed measurement and sorting of particles |
| US5273527A (en) | 1992-05-12 | 1993-12-28 | Ovamed Corporation | Delivery catheter |
| US5663048A (en) | 1990-10-04 | 1997-09-02 | University Of Calgary | Y-chromosome specific polynucleotide probes for prenatal sexing |
| US5840482A (en) | 1990-10-10 | 1998-11-24 | The Regents Of The University Of California | Y chromosome specific nucleic acid probe and method for determining the Y chromosome in situ |
| WO1992008120A1 (en) | 1990-10-29 | 1992-05-14 | Macquarie University | Pulsed laser flow cytometry |
| US5116125A (en) | 1990-10-31 | 1992-05-26 | Biophos Medical Ab | Fertility analyzer |
| DE69013366T2 (de) | 1990-10-31 | 1995-04-20 | Biophos Medical Ab | Fruchtbarkeitsanalysegerät. |
| JP2874746B2 (ja) | 1990-11-22 | 1999-03-24 | シスメックス株式会社 | フローイメージングサイトメータにおけるフローセル機構 |
| US5991028A (en) | 1991-02-22 | 1999-11-23 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for cell classification |
| JPH0734012B2 (ja) | 1991-02-27 | 1995-04-12 | 東亜医用電子株式会社 | フローイメージサイトメータ |
| JP3121849B2 (ja) | 1991-02-27 | 2001-01-09 | シスメックス株式会社 | フローイメージサイトメータ |
| US5144224A (en) | 1991-04-01 | 1992-09-01 | Larsen Lawrence E | Millimeter wave flow cytometer |
| US5199576A (en) | 1991-04-05 | 1993-04-06 | University Of Rochester | System for flexibly sorting particles |
| EP0515211A3 (en) | 1991-05-23 | 1993-04-07 | Becton Dickinson And Company | Apparatus and method for phase resolved fluorescence lifetimes of independent and varying amplitude pulses |
| DE9107792U1 (de) | 1991-06-25 | 1991-09-12 | Labotect-Labor-Technik, Göttingen, GmbH, 3406 Bovenden | Instrumentensatz zum uterinen Embryonentransfer |
| FR2678506B1 (fr) | 1991-07-01 | 2000-03-10 | Claude Ranoux | Procede de fecondation en cycle spontane. |
| US5548661A (en) | 1991-07-12 | 1996-08-20 | Price; Jeffrey H. | Operator independent image cytometer |
| US5412466A (en) | 1991-07-26 | 1995-05-02 | Toa Medical Electronics Co., Ltd. | Apparatus for forming flattened sample flow for analyzing particles |
| US5488469A (en) | 1991-08-30 | 1996-01-30 | Omron Corporation | Cell analyzing apparatus |
| DE69230902D1 (de) | 1991-08-30 | 2000-05-18 | Omron Tateisi Electronics Co | Vorrichtung zur Zellenanalyse |
| US5548395A (en) | 1991-09-20 | 1996-08-20 | Toa Medical Electronics Co., Ltd. | Particle analyzer |
| US5578449A (en) | 1991-10-03 | 1996-11-26 | Hilding Ohlsson, S.A. | Procedure for the sex determination of embryos in mammals especially applied to bovine embryos |
| US5919621A (en) | 1991-10-24 | 1999-07-06 | Brown; David B. | Methods for diagnosing human male infertility |
| JP3212647B2 (ja) | 1991-10-24 | 2001-09-25 | シスメックス株式会社 | イメージングフローサイトメータ |
| US5866344A (en) | 1991-11-15 | 1999-02-02 | Board Of Regents, The University Of Texas System | Antibody selection methods using cell surface expressed libraries |
| US5370842A (en) | 1991-11-29 | 1994-12-06 | Canon Kabushiki Kaisha | Sample measuring device and sample measuring system |
| JP2593021B2 (ja) | 1991-12-13 | 1997-03-19 | 伊藤ハム株式会社 | ウシ胚の性の識別方法 |
| JP3130628B2 (ja) | 1992-01-30 | 2001-01-31 | シスメックス株式会社 | 粒子判定装置 |
| US5400179A (en) | 1992-02-18 | 1995-03-21 | Asahi Kogaku Kogyo Kabushiki Kaisha | Optical multilayer thin film and beam splitter |
| DE69321748T2 (de) | 1992-02-20 | 1999-06-17 | Canon K.K., Tokio/Tokyo | Verfahren und Messapparatur zur Handhabung von Teilchen |
| JP3248910B2 (ja) | 1992-02-21 | 2002-01-21 | イギリス国 | 粒子特性の分析 |
| US5558998A (en) | 1992-02-25 | 1996-09-24 | The Regents Of The Univ. Of California | DNA fragment sizing and sorting by laser-induced fluorescence |
| US6120735A (en) | 1992-02-26 | 2000-09-19 | The Ohio States University | Fractional cell sorter |
| JPH084601B2 (ja) * | 1992-04-10 | 1996-01-24 | 岡山県 | 精子凍結方法及び精子凍結装置 |
| US5298967A (en) | 1992-06-02 | 1994-03-29 | Pacific Scientific Company | Measurement of concentrations of dissolved solvent |
| JP3145486B2 (ja) | 1992-06-12 | 2001-03-12 | シスメックス株式会社 | イメージングフローサイトメータ |
| AT406638B (de) | 1992-07-13 | 2000-07-25 | Pall Corp | Automatisiertes behandlungssystem für biologische fluide und verfahren hiezu |
| JP3215175B2 (ja) | 1992-08-10 | 2001-10-02 | シスメックス株式会社 | 粒子分析装置 |
| US5315122A (en) | 1992-08-25 | 1994-05-24 | Becton, Dickinson And Company | Apparatus and method for fluorescent lifetime measurement |
| US5466572A (en) | 1992-09-03 | 1995-11-14 | Systemix, Inc. | High speed flow cytometric separation of viable cells |
| AU681597B2 (en) | 1992-09-03 | 1997-09-04 | Regents Of The University Of California, The | High speed flow cytometric separation of viable mammalian cells |
| US5736410A (en) | 1992-09-14 | 1998-04-07 | Sri International | Up-converting reporters for biological and other assays using laser excitation techniques |
| US5275933A (en) | 1992-09-25 | 1994-01-04 | The Board Of Trustees Of The Leland Stanford Junior University | Triple gradient process for recovering nucleated fetal cells from maternal blood |
| US5371585A (en) | 1992-11-10 | 1994-12-06 | Pacific Scientific Company | Particle detecting instrument with sapphire detecting cell defining a rectangular flow path |
| US5359907A (en) | 1992-11-12 | 1994-11-01 | Horiba Instruments, Inc. | Method and apparatus for dry particle analysis |
| US5395588A (en) | 1992-12-14 | 1995-03-07 | Becton Dickinson And Company | Control of flow cytometer having vacuum fluidics |
| GB2290668B (en) | 1993-01-16 | 1997-07-23 | James Andrew Bangham | Signal processing system |
| JP2525713B2 (ja) | 1993-01-19 | 1996-08-21 | 農林水産省畜産試験場長 | 低分子チオ―ル化合物を用いた牛胚の培養法および輸送法 |
| US5467189A (en) | 1993-01-22 | 1995-11-14 | Venturedyne, Ltd. | Improved particle sensor and method for assaying a particle |
| JP3052665B2 (ja) | 1993-01-26 | 2000-06-19 | 株式会社日立製作所 | フローセル装置 |
| CA2113957A1 (en) | 1993-01-29 | 1994-07-30 | University Of Guelph | Nucleotide sequences for bovine sex determination |
| CA2155186A1 (en) | 1993-02-01 | 1994-08-18 | Kevin M. Ulmer | Methods and apparatus for dna sequencing |
| US5453575A (en) | 1993-02-01 | 1995-09-26 | Endosonics Corporation | Apparatus and method for detecting blood flow in intravascular ultrasonic imaging |
| US5367474A (en) | 1993-02-08 | 1994-11-22 | Coulter Corporation | Flow cytometer |
| US5547849A (en) | 1993-02-17 | 1996-08-20 | Biometric Imaging, Inc. | Apparatus and method for volumetric capillary cytometry |
| US5556764A (en) | 1993-02-17 | 1996-09-17 | Biometric Imaging, Inc. | Method and apparatus for cell counting and cell classification |
| US5563059A (en) | 1993-02-23 | 1996-10-08 | Genentech, Inc. | Use of human inhibin and human activin to increase the number of mature primate oocytes |
| EP0648348B1 (en) | 1993-03-01 | 1999-04-28 | General Signal Corporation | Variable annular illuminator for photolithographic projection imager. |
| NO930980L (no) | 1993-03-18 | 1994-09-19 | Flowtech As | Optisk konfigurasjon for væskeströmcytofotometer |
| US5658751A (en) | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5494795A (en) | 1993-05-05 | 1996-02-27 | The United States Of America As Represented By The Secretary Of The Navy | Specific oligonucleotide primers for detection of pathogenic campylobacter bacteria by polymerase chain reaction |
| US5439578A (en) | 1993-06-03 | 1995-08-08 | The Governors Of The University Of Alberta | Multiple capillary biochemical analyzer |
| EP0627643B1 (en) | 1993-06-03 | 1999-05-06 | Hamamatsu Photonics K.K. | Laser scanning optical system using axicon |
| PL307305A1 (en) | 1993-06-04 | 1995-05-15 | Kwahak International Co Ltd | Apparatus for arificial fertilisation and transfer of embryos |
| NO932088L (no) | 1993-06-08 | 1995-01-05 | Oddbjoern Gjelsnes | Anordning for anvendelse ved væskeströmscytometri |
| US5483469A (en) | 1993-08-02 | 1996-01-09 | The Regents Of The University Of California | Multiple sort flow cytometer |
| US5464581A (en) | 1993-08-02 | 1995-11-07 | The Regents Of The University Of California | Flow cytometer |
| US6328071B1 (en) | 1993-08-06 | 2001-12-11 | Cary Austin | Well pressure tank |
| DE69434551T2 (de) | 1993-09-16 | 2006-06-14 | Sysmex Corp | Teilchen-Analysegerät |
| US5503994A (en) | 1993-10-08 | 1996-04-02 | The Board Of Trustees Of The Leland Stanford Junior University | System for sample detection with compensation for difference in sensitivity to detection of components moving at different velocities |
| US5480774A (en) | 1993-10-14 | 1996-01-02 | A/F Protein, Inc. | Determination of genomic sex in salmonids |
| JP3290786B2 (ja) | 1993-11-26 | 2002-06-10 | シスメックス株式会社 | 粒子分析装置 |
| GB9324938D0 (en) | 1993-12-04 | 1994-01-26 | Atomic Energy Authority Uk | Aerosol generator |
| FI96452C (fi) | 1994-01-26 | 1996-06-25 | Pekka Haenninen | Menetelmä väriaineiden virittämiseksi |
| EP0748316B1 (en) | 1994-02-28 | 2002-05-08 | Sunkyong Industries Co., Ltd. | Pyrimidine acyclonucleoside derivatives |
| KR100402564B1 (ko) | 1994-03-25 | 2003-10-22 | 마루킨 츄유 가부시키가이샤 | 에피머라제 |
| US5475487A (en) | 1994-04-20 | 1995-12-12 | The Regents Of The University Of California | Aqueous carrier waveguide in a flow cytometer |
| DE4414940C2 (de) | 1994-04-28 | 1998-07-02 | Pekka Haenninen | Lumineszenz-Rastermikroskop mit zwei Photonen Anregung |
| US5595866A (en) | 1994-05-27 | 1997-01-21 | Methodist Hospital Of Indiana, Inc. | Step-wise method to remove cryoprotectant from sperm |
| DE4419894A1 (de) | 1994-06-07 | 1995-12-14 | Gip Medizin Technik Gmbh | Endoskopische Punktionsnadelvorrichtung |
| EP0687956B2 (de) | 1994-06-17 | 2005-11-23 | Carl Zeiss SMT AG | Beleuchtungseinrichtung |
| US5601234A (en) | 1994-08-01 | 1997-02-11 | Abbott Laboratories | Fluid nozzle and method of introducing a fluid |
| US5891734A (en) | 1994-08-01 | 1999-04-06 | Abbott Laboratories | Method for performing automated analysis |
| JP3375203B2 (ja) | 1994-08-08 | 2003-02-10 | シスメックス株式会社 | 細胞分析装置 |
| FR2723735B1 (fr) | 1994-08-18 | 1996-10-31 | Abx Sa | Boitier a branchement automatique pour distribution de reactifs dans un appareil notamment un analyseur hematologique. |
| US20020186874A1 (en) | 1994-09-07 | 2002-12-12 | Jeffrey H. Price | Method and means for image segmentation in fluorescence scanning cytometry |
| US5790692A (en) | 1994-09-07 | 1998-08-04 | Jeffrey H. Price | Method and means of least squares designed filters for image segmentation in scanning cytometry |
| US5700692A (en) | 1994-09-27 | 1997-12-23 | Becton Dickinson And Company | Flow sorter with video-regulated droplet spacing |
| US5934885A (en) | 1994-10-07 | 1999-08-10 | Bayer Corporation | Reagent pump assembly |
| IL115327A (en) | 1994-10-07 | 2000-08-13 | Bayer Ag | Diaphragm pump |
| US5602349A (en) | 1994-10-14 | 1997-02-11 | The University Of Washington | Sample introduction system for a flow cytometer |
| US6861265B1 (en) | 1994-10-14 | 2005-03-01 | University Of Washington | Flow cytometer droplet formation system |
| US5643796A (en) | 1994-10-14 | 1997-07-01 | University Of Washington | System for sensing droplet formation time delay in a flow cytometer |
| DE69532416T2 (de) | 1994-10-14 | 2004-12-02 | University Of Washington, Seattle | Methode und vorrichtung zur tröpfchenerzeugung mit hoher rate bei der durchfluss-cytometrie |
| US5602039A (en) | 1994-10-14 | 1997-02-11 | The University Of Washington | Flow cytometer jet monitor system |
| US5495719A (en) | 1994-11-14 | 1996-03-05 | Gray, Jr.; Carl O. | Method of preserving spermatozoa |
| JP3347495B2 (ja) | 1994-11-14 | 2002-11-20 | シスメックス株式会社 | 粒子分析装置 |
| US5514537A (en) | 1994-11-28 | 1996-05-07 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Process and apparatus for sorting spermatozoa |
| DE69530072T2 (de) | 1994-12-08 | 2004-03-04 | Molecular Dynamics, Sunnyvale | System zur fluoreszenzabbildung unter verwendung eines objektivs mit makroabtastung |
| US5632754A (en) | 1994-12-23 | 1997-05-27 | Devices For Vascular Intervention | Universal catheter with interchangeable work element |
| DE69533469T2 (de) | 1994-12-26 | 2005-09-22 | Sysmex Corp. | Durchflusszytometer |
| US5835262A (en) | 1994-12-28 | 1998-11-10 | Research Development Corporation Of Japan | Multi-wavelength optical microscope |
| FI98765C (fi) | 1995-01-16 | 1997-08-11 | Erkki Soini | Virtaussytometrinen menetelmä ja laite |
| US5608519A (en) | 1995-03-20 | 1997-03-04 | Gourley; Paul L. | Laser apparatus and method for microscopic and spectroscopic analysis and processing of biological cells |
| US5793485A (en) | 1995-03-20 | 1998-08-11 | Sandia Corporation | Resonant-cavity apparatus for cytometry or particle analysis |
| US5620842A (en) | 1995-03-29 | 1997-04-15 | Becton Dickinson And Company | Determination of the number of fluorescent molecules on calibration beads for flow cytometry |
| US5786560A (en) | 1995-03-31 | 1998-07-28 | Panasonic Technologies, Inc. | 3-dimensional micromachining with femtosecond laser pulses |
| US5699152A (en) | 1995-04-03 | 1997-12-16 | Alltrista Corporation | Electro-optical inspection system and method |
| US5682038A (en) | 1995-04-06 | 1997-10-28 | Becton Dickinson And Company | Fluorescent-particle analyzer with timing alignment for analog pulse subtraction of fluorescent pulses arising from different excitation locations |
| US5528045A (en) | 1995-04-06 | 1996-06-18 | Becton Dickinson And Company | Particle analyzer with spatially split wavelength filter |
| US5641457A (en) | 1995-04-25 | 1997-06-24 | Systemix | Sterile flow cytometer and sorter with mechanical isolation between flow chamber and sterile enclosure |
| US5687727A (en) | 1995-05-01 | 1997-11-18 | Danforth Biomedical Incorporated | Catheter adaptor with slitting blade and improved manual control and method of use |
| CA2220550C (en) | 1995-05-09 | 2001-04-17 | Curators Of The University Of Missouri | A system for introducing a fluid into the uterus of an animal |
| FR2734637B1 (fr) | 1995-05-24 | 1997-08-14 | Abx Sa | Dispositif d'inspection optique d'un fluide, notamment pour analyses hematologiques |
| US5798276A (en) | 1995-06-07 | 1998-08-25 | Molecular Probes, Inc. | Reactive derivatives of sulforhodamine 101 with enhanced hydrolytic stability |
| US5650847A (en) | 1995-06-14 | 1997-07-22 | Erkki Soini | Method and device for determination of parameters of individual microparticles |
| US6454945B1 (en) | 1995-06-16 | 2002-09-24 | University Of Washington | Microfabricated devices and methods |
| US5716852A (en) | 1996-03-29 | 1998-02-10 | University Of Washington | Microfabricated diffusion-based chemical sensor |
| US5589457A (en) | 1995-07-03 | 1996-12-31 | Ausa International, Inc. | Process for the synchronization of ovulation |
| US6411835B1 (en) | 1997-01-13 | 2002-06-25 | Medispectra, Inc. | Spectral volume microprobe arrays |
| BR9610235A (pt) | 1995-08-11 | 1999-07-27 | Univ Guelph | Processos para identificar moléculas especificas e não especificas do sexo para tirar uma molécula específica do sexo para diferenciar entre machos e fêmeas para separar esperma determinado sexo mascukino ou feminino de sêmen nativo e para preparar uma preparação enriquecida par moléculas n o especificas do sexo feminino e do masculino anticorpo contra um epítopo de uma molécula específica do sexo molécula especifica do sexo isolada e purificada kit contraceptivo e uso de uma molécula n o especifica do sexo. |
| CA2231114A1 (en) | 1995-09-06 | 1997-03-13 | The Research Foundation Of State University Of New York | Two-photon upconverting dyes and applications |
| DE19533092A1 (de) | 1995-09-07 | 1997-03-13 | Basf Ag | Vorrichtung zur parallelisierten Zweiphotonen-Fluoreszenz-Korrelations-Spektroskopie (TPA-FCS) und deren Verwendung zum Wirkstoff-Screening |
| US6329158B1 (en) | 1995-09-15 | 2001-12-11 | Becton Dickinson And Company | Use of dimly fluorescing nucleic acid dyes in the identification of nucleated cells |
| US6040139A (en) | 1995-09-19 | 2000-03-21 | Bova; G. Steven | Laser cell purification system |
| US5726751A (en) | 1995-09-27 | 1998-03-10 | University Of Washington | Silicon microchannel optical flow cytometer |
| US5780230A (en) | 1995-10-06 | 1998-07-14 | Coriell Institute For Medical Research | Compositions and methods for human sperm activation and quantitative assessment of human sperm genome replication |
| US5736330A (en) | 1995-10-11 | 1998-04-07 | Luminex Corporation | Method and compositions for flow cytometric determination of DNA sequences |
| US6117068A (en) | 1995-10-19 | 2000-09-12 | Elite Genetics, Inc | Artificial insemination system |
| CA2235069C (en) | 1995-10-19 | 2010-12-14 | Advanced Reproduction Technologies, Inc. | Methods and compositions to improve germ cell and embryo survival and function |
| AU722176B2 (en) | 1995-11-09 | 2000-07-27 | Medical Research Council | Use of CYB medium for the transportation and storage of sperm |
| DE19549015C1 (de) | 1995-12-28 | 1997-04-03 | Siemens Ag | Verfahren und Anordnung zur Überwachung eines abreißenden Flüssigkeitstrahls |
| JP3584108B2 (ja) | 1996-01-08 | 2004-11-04 | キヤノン株式会社 | レンズ鏡筒 |
| US6641708B1 (en) | 1996-01-31 | 2003-11-04 | Board Of Regents, The University Of Texas System | Method and apparatus for fractionation using conventional dielectrophoresis and field flow fractionation |
| WO1997029354A1 (de) | 1996-02-05 | 1997-08-14 | Bayer Aktiengesellschaft | Verfahren und vorrichtung zum sortieren und zur gewinnung von planar ausgebrachten biologischen objekten wie biologische zellen bzw. zellorganellen, histologischen schnitten, chromosomenteilchen etc. mit laserstrahlen |
| BR9600722A (pt) | 1996-02-14 | 1997-12-30 | Jorge Antonio Rodrigues Claro | Bomba de infusão para contidos em bolsas plásticas flexíveis |
| AU1852297A (en) | 1996-02-16 | 1997-09-02 | Inphocyte, Inc. | System and method for rapid analysis of cells using spectral cytometry |
| JP3127111B2 (ja) | 1996-02-22 | 2001-01-22 | 株式会社日立製作所 | フロー式粒子画像解析方法および装置 |
| US6143901A (en) | 1996-07-31 | 2000-11-07 | Genesoft, Inc. | Complex formation between dsDNA and pyrrole imidazole polyamides |
| US6090947A (en) | 1996-02-26 | 2000-07-18 | California Institute Of Technology | Method for the synthesis of pyrrole and imidazole carboxamides on a solid support |
| US5895922A (en) | 1996-03-19 | 1999-04-20 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence | Fluorescent biological particle detection system |
| US5701012A (en) | 1996-03-19 | 1997-12-23 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence | Fluorescent biological particle detection system |
| US5747349A (en) | 1996-03-20 | 1998-05-05 | University Of Washington | Fluorescent reporter beads for fluid analysis |
| JP3590470B2 (ja) | 1996-03-27 | 2004-11-17 | アルプス電気株式会社 | 洗浄水生成方法および洗浄方法ならびに洗浄水生成装置および洗浄装置 |
| JP3640461B2 (ja) | 1996-04-03 | 2005-04-20 | シスメックス株式会社 | 粒子分析装置 |
| US5707808A (en) | 1996-04-15 | 1998-01-13 | The Regents Of The University Of California | Optical selection and collection of DNA fragments |
| JP2000510582A (ja) | 1996-04-25 | 2000-08-15 | ゼニコン・サイエンシーズ・コーポレーション | 微粒子標識を使用した分析物アッセイ |
| WO1997043620A1 (en) | 1996-05-15 | 1997-11-20 | International Remote Imaging Systems, Inc. | Selectively emphasizing particles of interest from a fluid sample for analysis |
| JP2963393B2 (ja) | 1996-06-14 | 1999-10-18 | 浜松ホトニクス株式会社 | 光電子増倍管用電圧分割回路 |
| US5846737A (en) | 1996-07-26 | 1998-12-08 | Molecular Probes, Inc. | Conjugates of sulforhodamine fluorophores with enhanced fluorescence |
| US5909278A (en) | 1996-07-29 | 1999-06-01 | The Regents Of The University Of California | Time-resolved fluorescence decay measurements for flowing particles |
| US5872627A (en) | 1996-07-30 | 1999-02-16 | Bayer Corporation | Method and apparatus for detecting scattered light in an analytical instrument |
| US5883378A (en) | 1996-07-30 | 1999-03-16 | Bayer Corporation | Apparatus and methods for transmitting electrical signals indicative of optical interactions between a light beam and a flowing suspension of particles |
| US6042249A (en) | 1996-07-30 | 2000-03-28 | Bayer Corporation | Illuminator optical assembly for an analytical instrument and methods of alignment and manufacture |
| US5719667A (en) | 1996-07-30 | 1998-02-17 | Bayer Corporation | Apparatus for filtering a laser beam in an analytical instrument |
| EP0822401A3 (en) | 1996-07-30 | 1999-05-06 | Bayer Corporation | Hydraulic system for a hematology analytical instrument |
| EP0822404B1 (en) | 1996-07-30 | 2009-06-17 | Siemens Healthcare Diagnostics Inc. | Optical system for a hematology analytical instrument |
| US6074827A (en) | 1996-07-30 | 2000-06-13 | Aclara Biosciences, Inc. | Microfluidic method for nucleic acid purification and processing |
| US5844685A (en) | 1996-07-30 | 1998-12-01 | Bayer Corporation | Reference laser beam sampling apparatus |
| US5745308A (en) | 1996-07-30 | 1998-04-28 | Bayer Corporation | Methods and apparatus for an optical illuminator assembly and its alignment |
| US5998140A (en) | 1996-07-31 | 1999-12-07 | The Scripps Research Institute | Complex formation between dsDNA and oligomer of cyclic heterocycles |
| US5804436A (en) | 1996-08-02 | 1998-09-08 | Axiom Biotechnologies, Inc. | Apparatus and method for real-time measurement of cellular response |
| US5919360A (en) | 1996-08-07 | 1999-07-06 | Cuno, Inc. | Additive dispensing apparatus |
| DK0925494T3 (da) | 1996-09-04 | 2002-07-01 | Scandinavian Micro Biodevices | Mikrostrømningssystem til partikelseparation og analyse |
| EP0929300B1 (en) | 1996-09-06 | 2004-05-26 | Medarex, Inc. | Cyanidin compositions and therapeutic and diagnostic uses therefor |
| US5873254A (en) | 1996-09-06 | 1999-02-23 | Interface Multigrad Technology | Device and methods for multigradient directional cooling and warming of biological samples |
| US6221654B1 (en) | 1996-09-25 | 2001-04-24 | California Institute Of Technology | Method and apparatus for analysis and sorting of polynucleotides based on size |
| US5759767A (en) | 1996-10-11 | 1998-06-02 | Joseph R. Lakowicz | Two-photon and multi-photon measurement of analytes in animal and human tissues and fluids |
| US6002471A (en) | 1996-11-04 | 1999-12-14 | California Institute Of Technology | High resolution scanning raman microscope |
| US5799830A (en) | 1996-11-08 | 1998-09-01 | Carroll; David C. | Pressure vessel access port |
| US5696157A (en) | 1996-11-15 | 1997-12-09 | Molecular Probes, Inc. | Sulfonated derivatives of 7-aminocoumarin |
| EP2264427B1 (en) | 1997-01-31 | 2017-05-03 | Xy, Llc | Optical apparatus with focussing reflector for converging radiation onto a flow of particles, and related method of analysis |
| US6753161B2 (en) | 1997-03-27 | 2004-06-22 | Oncosis Llc | Optoinjection methods |
| US6534308B1 (en) | 1997-03-27 | 2003-03-18 | Oncosis, Llc | Method and apparatus for selectively targeting specific cells within a mixed cell population |
| US5874266A (en) | 1997-03-27 | 1999-02-23 | Palsson; Bernhard O. | Targeted system for removing tumor cells from cell populations |
| GB9707096D0 (en) | 1997-04-08 | 1997-05-28 | Smithkline Beecham Plc | Novel device |
| JP2968231B2 (ja) | 1997-04-11 | 1999-10-25 | 株式会社荏原製作所 | 空調システム |
| US6050935A (en) | 1997-05-09 | 2000-04-18 | Biofertec | Container assembly for intravaginal fertilization and culture and embryo transfer and method of intravaginal fertilization and culture employing such a container |
| US6133995A (en) | 1997-05-09 | 2000-10-17 | Sysmex Corporation | Particle measuring apparatus |
| EP0988523B1 (en) | 1997-06-09 | 2013-08-14 | EMD Millipore Corporation | Method and apparatus for detecting microparticles in fluid samples |
| US6139800A (en) | 1997-06-23 | 2000-10-31 | Luminex Corporation | Interlaced lasers for multiple fluorescence measurement |
| DE69834527T2 (de) | 1997-07-01 | 2007-05-10 | VLP Watertown Limited Partnership, Watertown | Verfahren zur Geschlechtsbestimmung von Säuger-Nachkommenschaft |
| US6111398A (en) | 1997-07-03 | 2000-08-29 | Coulter International Corp. | Method and apparatus for sensing and characterizing particles |
| BR9704313A (pt) | 1997-07-08 | 1999-04-06 | Alves Elias Walter | Utilização de ig-y de ovo de galinha associado a anticorpos monoclonais contra antigenos sexo especifico na imunosexagem de espermatozóides de bovinos |
| US5899848A (en) | 1997-07-14 | 1999-05-04 | Haubrich; Mark A. | Device and process for artificial insemination of animals |
| US5985216A (en) | 1997-07-24 | 1999-11-16 | The United States Of America, As Represented By The Secretary Of Agriculture | Flow cytometry nozzle for high efficiency cell sorting |
| US5876942A (en) | 1997-07-24 | 1999-03-02 | National Science Council Of Republic Of China | Process for sexing cow embryos |
| US5985538A (en) | 1997-08-01 | 1999-11-16 | Saint Barnabas Medical Center | Cryopreservation and cell culture medium comprising less than 50 mM sodium ions and greater than 100 mM choline salt |
| US6003678A (en) | 1997-08-21 | 1999-12-21 | University Of Washington | Particle separating apparatus and method |
| US5819948A (en) | 1997-08-21 | 1998-10-13 | Van Den Engh; Gerrit J. | Particle separating apparatus and method |
| US5880474A (en) | 1997-08-29 | 1999-03-09 | Becton Dickinson And Company | Multi-illumination-source flow particle analyzer with inter-location emissions crosstalk cancelation |
| CA2304197C (en) | 1997-09-22 | 2009-02-03 | University Of Guelph | Reduction of sperm sensitivity to chilling |
| US6540895B1 (en) | 1997-09-23 | 2003-04-01 | California Institute Of Technology | Microfabricated cell sorter for chemical and biological materials |
| US6322901B1 (en) | 1997-11-13 | 2001-11-27 | Massachusetts Institute Of Technology | Highly luminescent color-selective nano-crystalline materials |
| US6201628B1 (en) | 1997-11-19 | 2001-03-13 | University Of Washington | High throughput optical scanner |
| US6086574A (en) | 1997-11-21 | 2000-07-11 | Hyclone Laboratories, Inc. | Fluid delivery systems with diptube connector |
| US5895764A (en) | 1997-11-24 | 1999-04-20 | University Of New Mexico | Controlled sheath flow injection cytometry |
| US6207392B1 (en) | 1997-11-25 | 2001-03-27 | The Regents Of The University Of California | Semiconductor nanocrystal probes for biological applications and process for making and using such probes |
| US5990479A (en) | 1997-11-25 | 1999-11-23 | Regents Of The University Of California | Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes |
| US6221671B1 (en) | 1997-12-12 | 2001-04-24 | Chemunex S.A. | Digital flow cytometer and method |
| US6071689A (en) | 1997-12-31 | 2000-06-06 | Xy, Inc. | System for improving yield of sexed embryos in mammals |
| US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
| EP1058688A4 (en) | 1998-02-03 | 2004-10-06 | Xy Inc | USE OF SPECIFIC OLIGONUCLEOTIDE PRIMERS TO DETECT THE PRESENCE OF MALE BOVINE CHROMOSOME BY CHAIN AMPLIFICATION BY POLYMERASE |
| US6746873B1 (en) | 1998-02-20 | 2004-06-08 | Xy, Inc. | Vibratory system for a sorting flow cytometer |
| EP1062494B1 (en) | 1998-02-20 | 2015-06-24 | Xy, Llc | A vibratory system for a sorting flow cytometer |
| US6154276A (en) | 1998-02-23 | 2000-11-28 | The Regents Of The University Of California | Waveguide detection of right-angle-scattered light in flow cytometry |
| US6211477B1 (en) | 1998-02-26 | 2001-04-03 | Becton Dickinson And Company | Electrostatic deceleration system for flow cytometer |
| US6248590B1 (en) | 1998-02-27 | 2001-06-19 | Cytomation, Inc. | Method and apparatus for flow cytometry |
| US6042025A (en) | 1998-03-13 | 2000-03-28 | Smith Et Al. | Two hole dispenser with baffles |
| ATE254762T1 (de) | 1998-03-16 | 2003-12-15 | Partec Partikelzaehlgeraete Gm | Elektronisches gerät zur präzisen abgabe kleiner flüssigkeitsmengen |
| JP3594794B2 (ja) | 1998-03-24 | 2004-12-02 | 独立行政法人 科学技術振興機構 | ナノ秒時間ゲート分光診断装置 |
| US6642018B1 (en) * | 1998-03-27 | 2003-11-04 | Oncosis Llc | Method for inducing a response in one or more targeted cells |
| NZ506887A (en) | 1998-03-30 | 2003-12-19 | Bioshaf Ltd | Flow cytometer analysis of cells and body fluids for fertility testing |
| US6175409B1 (en) | 1999-04-02 | 2001-01-16 | Symyx Technologies, Inc. | Flow-injection analysis and variable-flow light-scattering methods and apparatus for characterizing polymers |
| JP3350442B2 (ja) | 1998-04-09 | 2002-11-25 | 科学技術振興事業団 | 顕微鏡システム |
| CA2328408C (en) | 1998-05-14 | 2005-02-15 | Luminex Corporation | Zero dead time architecture and method for flow cytometer |
| CA2320296A1 (en) | 1998-05-18 | 1999-11-25 | University Of Washington | Liquid analysis cartridge |
| ATE530891T1 (de) | 1998-05-22 | 2011-11-15 | California Inst Of Techn | Miniaturisierter zellsortierer |
| US6079836A (en) | 1998-07-20 | 2000-06-27 | Coulter International Corp. | Flow cytometer droplet break-off location adjustment mechanism |
| PT1917974E (pt) | 1998-07-30 | 2011-02-22 | Xy Llc | Sistema de inseminação artificial não cirúrgica em equinos |
| US20040107150A1 (en) | 1998-07-31 | 2004-06-03 | Chata Biosystems, Inc. Of Colorado | Apparatus and method with vessel for containing/transporting a fluent substance |
| US6729369B2 (en) | 1998-07-31 | 2004-05-04 | Chata Biosystems, Inc. | Vessel for containing/transporting a fluent substance |
| DE29813921U1 (de) | 1998-08-04 | 1998-10-08 | Kisfeld, Alfons, 48691 Vreden | Vorrichtung zur Besamung von Tieren, insbesondere Sauen |
| US6309815B1 (en) * | 1998-08-07 | 2001-10-30 | University Of Kansas Medical Center | Composition and method for preparation, storage and activation of large populations of immotile sperm |
| US6087352A (en) | 1998-08-17 | 2000-07-11 | Trout; William E. | Use of Zeranol to modulate reproductive cycles |
| CA2341231C (en) | 1998-08-21 | 2003-10-21 | Union Biometrica, Inc. | Instrument for selecting and depositing multicellular organisms and other large objects |
| US6326144B1 (en) | 1998-09-18 | 2001-12-04 | Massachusetts Institute Of Technology | Biological applications of quantum dots |
| US6495333B1 (en) | 1998-09-22 | 2002-12-17 | Becton Dickinson And Company | Flow cytometric, whole blood dendritic cell immune function assay |
| US6208411B1 (en) | 1998-09-28 | 2001-03-27 | Kla-Tencor Corporation | Massively parallel inspection and imaging system |
| US6707555B1 (en) | 1998-10-15 | 2004-03-16 | Sysmex Corporation | Optical information measuring apparatus |
| US6423505B1 (en) | 1998-12-03 | 2002-07-23 | Becton Dickinson And Company | Methods and reagents for quantitation of HLA-DR and CD11b expression on peripheral blood cells |
| US7116407B2 (en) | 1998-12-15 | 2006-10-03 | Union Biometrica, Inc. | System for axial pattern analysis of multicellular organisms |
| US6128133A (en) | 1998-12-22 | 2000-10-03 | Lucent Technologies Inc. | Optical beamsplitter |
| EP1018644A2 (en) | 1999-01-06 | 2000-07-12 | Bayer Corporation | Variable rate particle counter and method of use |
| US6256096B1 (en) | 1999-01-11 | 2001-07-03 | Softray | Flow cytometry apparatus and method |
| US20010006416A1 (en) | 1999-01-11 | 2001-07-05 | Johnson Paul E. | Ribbon flow cytometry apparatus and methods |
| US6150119A (en) | 1999-01-19 | 2000-11-21 | Caliper Technologies Corp. | Optimized high-throughput analytical system |
| US6671044B2 (en) | 1999-01-25 | 2003-12-30 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells in broad flat flow |
| US6580504B1 (en) | 1999-01-25 | 2003-06-17 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
| US6473176B2 (en) | 1999-01-25 | 2002-10-29 | Amnis Corporation | Imaging and analyzing parameters of small moving objects such as cells |
| US6119465A (en) | 1999-02-10 | 2000-09-19 | Mullens; Patrick L. | Shipping container for storing materials at cryogenic temperatures |
| FR2789778B1 (fr) | 1999-02-12 | 2001-09-14 | France Telecom | Procede pour associer des references d'acheminement a des paquets de donnees au moyen d'une memoire trie, et routeur de paquets appliquant ce procede |
| JP2000239101A (ja) * | 1999-02-18 | 2000-09-05 | Japan Science & Technology Corp | ラット精子の凍結保存法 |
| US6323632B1 (en) | 1999-08-13 | 2001-11-27 | Coulter International Corp. | Solid state RF oscillator-detector for flow cytometer |
| CH690557A5 (de) | 1999-03-01 | 2000-10-13 | Arson Ag | Beleuchtungseinrichtung. |
| US6097485A (en) | 1999-03-08 | 2000-08-01 | Integrated Waveguides, Inc. | Microchip optical transport technology for use in a personal flow cytometer |
| FR2791645B1 (fr) | 1999-04-02 | 2001-06-15 | Valois Sa | Echantillon de produit fluide destine a la presse |
| US6193647B1 (en) | 1999-04-08 | 2001-02-27 | The Board Of Trustees Of The University Of Illinois | Microfluidic embryo and/or oocyte handling device and method |
| US6793387B1 (en) | 1999-05-08 | 2004-09-21 | Chata Biosystems, Inc. | Apparatus for automatic preparation of a mixture and method |
| FR2793495B1 (fr) | 1999-05-14 | 2001-08-10 | Imv Technologies | Dilueur pour la cryoconservation de spermatozoides de bovins |
| FR2793708B1 (fr) | 1999-05-21 | 2001-08-03 | Valois Sa | Dispositif de distribution de produit fluide |
| US6372506B1 (en) | 1999-07-02 | 2002-04-16 | Becton, Dickinson And Company | Apparatus and method for verifying drop delay in a flow cytometer |
| JP3361778B2 (ja) * | 1999-07-09 | 2003-01-07 | 社団法人家畜改良事業団 | 家畜の人工授精用精子または受精卵移植用卵子の注入器及びその操作方法 |
| IT1307787B1 (it) | 1999-07-26 | 2001-11-19 | Univ Firenze | Processo per incrementare la motilita' degli spermatozoi e spermatozoia motilita' superiore cosi' ottenuti. |
| DE19935766A1 (de) | 1999-07-29 | 2001-02-01 | Friedrich Schiller Uni Jena Bu | Verfahren zur optischen Anregung von Fluorophor-markierter DNA und RNA |
| US6664550B2 (en) | 1999-08-30 | 2003-12-16 | Sandia National Laboratories | Apparatus to collect, classify, concentrate, and characterize gas-borne particles |
| US6495366B1 (en) | 1999-09-03 | 2002-12-17 | Therakos, Inc. | Uninterrupted flow pump apparatus and method |
| US6813017B1 (en) | 1999-10-20 | 2004-11-02 | Becton, Dickinson And Company | Apparatus and method employing incoherent light emitting semiconductor devices as particle detection light sources in a flow cytometer |
| EP1227898A4 (en) | 1999-10-21 | 2011-11-16 | Beckman Coulter Inc | SYSTEM FOR ANALYSIS BY FLOW CYTOMETRY WITH TEMPORARY DYNAMICS |
| US6472153B1 (en) | 1999-10-26 | 2002-10-29 | Epoch Biosciences, Inc. | Hybridization-triggered fluorescent detection of nucleic acids |
| US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
| CA2392534A1 (en) | 1999-11-30 | 2001-06-07 | Oncosis | Method and apparatus for selectively targeting specific cells within a cell population |
| US6263745B1 (en) | 1999-12-03 | 2001-07-24 | Xy, Inc. | Flow cytometer nozzle and flow cytometer sample handling methods |
| US6558911B1 (en) | 1999-12-10 | 2003-05-06 | Oregon Health Sciences University | Sperm quality assay |
| DE60014440T2 (de) | 2000-01-03 | 2005-11-17 | Ibérica de Reproducción Asistida, S.L. | Vorrichtung zur künstlichen befruchtung von schweinen |
| ES2174778T3 (es) | 2000-01-14 | 2002-11-16 | Exelixis Deutschland Gmbh | Procedimiento de crioconservacion de esperma de pez cebra. |
| US6465169B2 (en) | 2000-01-14 | 2002-10-15 | Artemis Pharmaceuticals Gmbh | Method for cryoconservation of Zebrafish sperm |
| IT1317724B1 (it) | 2000-01-14 | 2003-07-15 | Istituto Sperimentale Italiano | Procedimento per la produzione di embrioni non umani di sessopredeterminato ad alto valore genetico. |
| CA2398107C (en) | 2000-01-28 | 2013-11-19 | Althea Technologies, Inc. | Methods for analysis of gene expression |
| US6587203B2 (en) | 2000-02-17 | 2003-07-01 | Cornell Research Foundation, Inc. | Sort stream stabilizer for flow cytometer |
| US6618143B2 (en) | 2000-02-18 | 2003-09-09 | Idexx Laboratories, Inc. | High numerical aperture flow cytometer and method of using same |
| US6646742B1 (en) | 2000-02-19 | 2003-11-11 | Mwi, Inc. | Optical device and method for multi-angle laser light scatter |
| GB2360360B (en) | 2000-03-14 | 2002-03-20 | Univ Bristol | A method of sorting cells |
| JP3587755B2 (ja) | 2000-03-14 | 2004-11-10 | シスメックス株式会社 | 粒子測定装置およびその方法 |
| US6482652B2 (en) | 2000-03-23 | 2002-11-19 | The Board Of Trustees Of The Leland Stanford Junior University | Biological particle sorter |
| CA2403992A1 (en) | 2000-03-30 | 2001-10-11 | Max F. Rothschild | Genetic markers for improved meat characteristics in animals |
| JP2003530568A (ja) | 2000-04-11 | 2003-10-14 | シェモメテック・アクティーゼルスカブ | 試料の蛍光を検出するための方法および装置 |
| EP1147774A1 (en) | 2000-04-20 | 2001-10-24 | Stichting Dienst Landbouwkundig Onderzoek | Method for improving the quality of sperm for artificial insemination of animals |
| IL152714A (en) | 2000-05-09 | 2014-03-31 | Xy Llc | High-purity spermatozoa populations carrying chromosome-x and chromosome-y |
| AU2001274864A1 (en) | 2000-05-19 | 2001-12-03 | Cytomation, Inc. | A rapid multi-material sample input system |
| JP2001328901A (ja) * | 2000-05-19 | 2001-11-27 | Seatsuku Yoshitomi Kk | マウス精子凍結保存用キット |
| US6590911B1 (en) | 2000-06-02 | 2003-07-08 | Coherent, Inc. | Passively modelocked harmonic-generating laser |
| US6700130B2 (en) | 2001-06-29 | 2004-03-02 | Honeywell International Inc. | Optical detection system for flow cytometry |
| US6784981B1 (en) | 2000-06-02 | 2004-08-31 | Idexx Laboratories, Inc. | Flow cytometry-based hematology system |
| US7351376B1 (en) | 2000-06-05 | 2008-04-01 | California Institute Of Technology | Integrated active flux microfluidic devices and methods |
| US6503698B1 (en) | 2000-06-16 | 2003-01-07 | The United States Of America As Represented By The Secretary Of Agriculture | Cryopreservation of swine embryos |
| CN1633259A (zh) | 2000-06-12 | 2005-06-29 | Xy公司 | 利用分离的带x－染色体和带y－染色体的精子群的综合兽群管理系统 |
| DE10031028B4 (de) | 2000-06-26 | 2008-09-04 | Gnothis Holding Sa | Verfahren zur Selektion von Partikeln |
| GB0016920D0 (en) | 2000-07-10 | 2000-08-30 | Univ Cambridge Tech | Decondensation of DNA |
| US20040005582A1 (en) | 2000-08-10 | 2004-01-08 | Nanobiodynamics, Incorporated | Biospecific desorption microflow systems and methods for studying biospecific interactions and their modulators |
| NZ523911A (en) | 2000-08-10 | 2005-03-24 | Gtc Biotherapeutics Inc | Cryopreservation of sperm |
| FR2813283B1 (fr) | 2000-08-25 | 2003-02-14 | Valois Sa | Distributeur a pompe integree |
| EP1190684A1 (en) | 2000-09-05 | 2002-03-27 | Universiteit Gent | Device and method for artificial insemination of bovines and other animals |
| US20020028434A1 (en) | 2000-09-06 | 2002-03-07 | Guava Technologies, Inc. | Particle or cell analyzer and method |
| EP1354061A2 (en) | 2000-09-08 | 2003-10-22 | Iowa State University Research Foundation, Inc. | Novel prkag3 alleles and use of the same as genetic markers for reproductive and meat quality traits |
| WO2002023163A1 (en) | 2000-09-15 | 2002-03-21 | California Institute Of Technology | Microfabricated crossflow devices and methods |
| GB0023041D0 (en) | 2000-09-20 | 2000-11-01 | Univ Manchester | Identification apparatus |
| AU2001295086A1 (en) | 2000-09-27 | 2002-04-08 | Mark W. Bitensky | Cellular diagnostic arrays, methods of using and processes for producing same |
| CN1325909C (zh) | 2000-09-27 | 2007-07-11 | 清华大学 | 用于微粒操纵与微粒导向的装置及其使用方法 |
| BR0005045A (pt) | 2000-09-28 | 2002-05-14 | Marcos Fernando De Resende Mat | Método para sexagem de espermatozóides usando a via clássica do sistema complemento, com eliminação da via alternativa pela inativação da proteina "b" |
| AU2002211389A1 (en) | 2000-10-03 | 2002-04-15 | California Institute Of Technology | Microfluidic devices and methods of use |
| MXPA03002710A (es) | 2000-10-05 | 2004-01-26 | Xy Inc | Sistema de inseminacion histeroscopica de yeguas. |
| US20040031071A1 (en) | 2000-10-05 | 2004-02-12 | Xy, Inc. | System of hysteroscopic insemination of mares |
| WO2002031467A1 (en) | 2000-10-12 | 2002-04-18 | Amnis Corporation | Multipass cavity for illumination and excitation of moving objects |
| JP2004537712A (ja) | 2000-10-18 | 2004-12-16 | バーチャル・アレイズ・インコーポレーテッド | 多重細胞分析システム |
| MXPA03003556A (es) | 2000-10-23 | 2005-04-11 | Medical Instill Tech Inc | Distribuidor de fluido que tiene un frasco rigido y camara de aire interna flexible. |
| CA2428326A1 (en) | 2000-11-09 | 2002-05-16 | University Of Guelph | Mammalian sex selection using genetic modification |
| SE0004777D0 (sv) | 2000-12-22 | 2000-12-22 | Amersham Pharm Biotech Ab | Separation of X-and Y-sperm cells |
| CA2454340A1 (en) * | 2000-11-22 | 2002-05-30 | Pharmacia Corporation | Methods and apparatus for producing gender enriched sperm |
| WO2002043486A1 (en) | 2000-11-29 | 2002-06-06 | Xy, Inc. | System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations |
| US20020064808A1 (en) | 2000-11-29 | 2002-05-30 | Mutz Mitchell W. | Focused acoustic energy for ejecting cells from a fluid |
| US6849423B2 (en) | 2000-11-29 | 2005-02-01 | Picoliter Inc | Focused acoustics for detection and sorting of fluid volumes |
| US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
| US20020064809A1 (en) | 2000-11-29 | 2002-05-30 | Mutz Mitchell W. | Focused acoustic ejection cell sorting system and method |
| AU2002216063A1 (en) | 2000-11-30 | 2002-06-11 | The Netherlands Cancer Institute | Membrane molecule indicator compositions and methods |
| US6576291B2 (en) | 2000-12-08 | 2003-06-10 | Massachusetts Institute Of Technology | Preparation of nanocrystallites |
| US20040171163A1 (en) | 2000-12-15 | 2004-09-02 | Lopez Peter A. | Electrical conductive containment system |
| US6577387B2 (en) | 2000-12-29 | 2003-06-10 | Johnson & Johnson Vision Care, Inc. | Inspection of ophthalmic lenses using absorption |
| US6911450B1 (en) | 2001-01-29 | 2005-06-28 | Universite De Geneve | Pyrimidine acyclonucleoside derivatives, preparation method and use thereof |
| US6673095B2 (en) | 2001-02-12 | 2004-01-06 | Wound Healing Of Oklahoma, Inc. | Apparatus and method for delivery of laser light |
| US7029916B2 (en) | 2001-02-21 | 2006-04-18 | Maxcyte, Inc. | Apparatus and method for flow electroporation of biological samples |
| WO2002077011A2 (en) | 2001-03-12 | 2002-10-03 | The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon | Oxidation-reduction sensitive green fluorescent protein variants |
| US6706163B2 (en) | 2001-03-21 | 2004-03-16 | Michael Seul | On-chip analysis of particles and fractionation of particle mixtures using light-controlled electrokinetic assembly of particles near surfaces |
| CA2442019A1 (en) | 2001-03-22 | 2002-10-03 | Infigen, Inc. | Sex-specific selection of sperm from transgenic animals |
| EP1245944B1 (en) | 2001-03-29 | 2007-02-14 | Sysmex Corporation | Flow cytometer |
| CA2442282A1 (en) | 2001-03-29 | 2002-10-10 | Cellect Technologies Corp. | Methods devices and systems for sorting and separating particles |
| JP2002308897A (ja) | 2001-03-30 | 2002-10-23 | Council Scient Ind Res | 精子の運動不能性を引き起こす、インドのミミズの体腔液由来のタンパク質 |
| WO2002081934A2 (en) | 2001-04-03 | 2002-10-17 | Micronics, Inc. | Pneumatic valve interface for use in microfluidic structures |
| JP2002311027A (ja) | 2001-04-09 | 2002-10-23 | Hitachi Software Eng Co Ltd | ビーズ、ビーズ製造方法、フローサイトメータ及びプログラム |
| US6416190B1 (en) | 2001-04-27 | 2002-07-09 | University Of Chicago | Apparatus for using optical tweezers to manipulate materials |
| US20020186375A1 (en) | 2001-05-01 | 2002-12-12 | Asbury Charles L. | Device and methods for detecting samples in a flow cytometer independent of variations in fluorescence polarization |
| WO2002092161A1 (en) | 2001-05-10 | 2002-11-21 | Biophan, Llc | Miniaturized particle analyzer |
| US7345758B2 (en) | 2001-05-17 | 2008-03-18 | Cytopeia | Apparatus for analyzing and sorting biological particles |
| EP1395374B1 (en) | 2001-05-17 | 2013-04-17 | Beckman Coulter, Inc. | Flow cytometer with active automated optical alignment system |
| US20020182590A1 (en) | 2001-05-25 | 2002-12-05 | Vanderbilt University | Determining protein function in cell culture using RNA interference |
| US20030048433A1 (en) | 2001-06-01 | 2003-03-13 | Jean-Marie Desjonqueres | Cytometer signal processing system and method |
| FR2825987B1 (fr) | 2001-06-19 | 2003-12-12 | Valois Sa | Distributeur de produit fluide |
| WO2003008102A1 (en) | 2001-07-18 | 2003-01-30 | The Regents Of The University Of Michigan | Microfluidic gravity pump with constant flow rate |
| AU2002318269A1 (en) | 2001-07-18 | 2003-03-03 | The Regents Of The University Of Michigan | Gas-focusing flow cytometer cell and flow cytometer detection system with waveguide optics |
| FR2828281B1 (fr) | 2001-08-02 | 2004-12-31 | Biocytex | Dispositif pour l'analyse d'un echantillon notamment par cytometrie de flux |
| WO2003020877A2 (en) | 2001-09-01 | 2003-03-13 | Pharmacia Corporation | Staining and sorting genomes and chromosomes using sequence-specific polyamides |
| DE10151216A1 (de) | 2001-10-16 | 2003-04-24 | Zeiss Carl Jena Gmbh | Verfahren zur optischen Erfassung von charakteristischen Größen einer beleuchteten Probe |
| US20030078703A1 (en) | 2001-10-19 | 2003-04-24 | Surromed, Inc. | Cytometry analysis system and method using database-driven network of cytometers |
| US6838289B2 (en) | 2001-11-14 | 2005-01-04 | Beckman Coulter, Inc. | Analyte detection system |
| US6831279B2 (en) | 2001-11-27 | 2004-12-14 | Her Majesty The Queen In Right Of Canada, As Represented By The Minister Of National Defence | Laser diode-excited biological particle detection system |
| MXPA04006065A (es) | 2001-12-18 | 2004-09-27 | Thomson Licensing Sa | Sub-titulos y tele-texto generado en forma interna para menus de ajuste de un aparato de procesamiento de senal de capacidad de red. |
| AU2002361220A1 (en) | 2001-12-31 | 2003-07-15 | Institut Fur Physikalische Hochtechnologie E.V. | Micro-recess array for size-dependent sorting or separation of cells suspended in a flowing medium and method for analysing the functional activity of individual cells |
| WO2003056330A2 (de) | 2001-12-31 | 2003-07-10 | Institut für Physikalische Hochtechnologie e.V. | Zellsortiersystem zur grössenabhängigen sortierung oder separation von in einer strömenden flüssigkeit suspendierten zellen |
| WO2003062796A1 (en) | 2002-01-22 | 2003-07-31 | Dakocytomation Denmark A/S | Environmental containment system for a flow cytometer |
| US6698627B2 (en) | 2002-02-19 | 2004-03-02 | Valois S.A.S. | Fluid dispenser |
| US6849394B2 (en) * | 2002-02-21 | 2005-02-01 | Minitube Of America | Compositions comprising reproductive cell media and methods for using such compositions |
| AU2003219836A1 (en) | 2002-02-27 | 2003-09-09 | The Regents Of The University Of Michigan | Process for sorting motile particles from lesser-motile particles and apparatus suitable therefor |
| US20030175980A1 (en) | 2002-03-14 | 2003-09-18 | Hayenga Jon W. | Ribbon flow cytometry and cell sorting |
| US7223371B2 (en) | 2002-03-14 | 2007-05-29 | Micronics, Inc. | Microfluidic channel network device |
| JP2004000144A (ja) | 2002-03-29 | 2004-01-08 | Aisin Seiki Co Ltd | 細胞分離選別装置、細胞整列用基板 |
| US7312085B2 (en) | 2002-04-01 | 2007-12-25 | Fluidigm Corporation | Microfluidic particle-analysis systems |
| MXPA04012850A (es) | 2002-06-28 | 2005-02-24 | Monsanto Technology Llc | Sistema de negocios para genetica porcina. |
| AU2003255529B2 (en) | 2002-07-10 | 2008-11-20 | Laboratoires Serono Sa | Use of compounds for increasing spermatozoa motility |
| CA2531176C (en) | 2002-07-22 | 2018-04-24 | Xy, Inc. | Sperm cell process system |
| CN102539303B (zh) | 2002-07-31 | 2016-06-15 | 普瑞姆遗传(英国)有限公司 | 利用全息激光控制分类物质的系统和方法 |
| JP4595067B2 (ja) | 2002-08-01 | 2010-12-08 | エックスワイ，エルエルシー | 低圧精子細胞分離システム |
| CA2534394C (en) | 2002-08-15 | 2013-01-08 | Xy, Inc. | High resolution flow cytometer |
| US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
| JP3983151B2 (ja) | 2002-09-25 | 2007-09-26 | ダイワ精工株式会社 | 魚釣用スピニングリール |
| US20040061853A1 (en) | 2002-09-27 | 2004-04-01 | Blasenheim Barry J. | Prism-based flow cytometry excitation optics |
| US7201875B2 (en) | 2002-09-27 | 2007-04-10 | Becton Dickinson And Company | Fixed mounted sorting cuvette with user replaceable nozzle |
| US6941005B2 (en) | 2002-11-01 | 2005-09-06 | Coulter International Corp. | Monitoring and control of droplet sorting |
| EP1565737A4 (en) | 2002-11-20 | 2007-02-14 | Univ Virginia | SEPARATION OF SPERM CELLS FROM OTHER BIOLOGICAL SUBSTANCES USING MICRO-PRODUCED EQUIPMENT, AND RELATED METHODS |
| AU2003297304A1 (en) | 2002-12-19 | 2004-07-22 | Monsanto Technology Llc | Method and means for early detection of pregnancy in animals by combination testing |
| BRPI0408857B1 (pt) | 2003-03-28 | 2018-09-11 | Inguran Llc | aparelho, métodos e processos para separar partículas e para prover esperma de animal separado por sexo |
| WO2004087177A1 (en) * | 2003-03-28 | 2004-10-14 | Monsanto Technology Llc | Process for the staining of sperm |
| ES2541121T3 (es) | 2003-05-15 | 2015-07-16 | Xy, Llc | Clasificación eficiente de células haploides por sistemas de citometría de flujo |
| US20050011582A1 (en) | 2003-06-06 | 2005-01-20 | Haug Jeffrey S. | Fluid delivery system for a flow cytometer |
| US7460223B2 (en) | 2003-09-19 | 2008-12-02 | Applied Biosystems Inc. | Inverted orientation for a microplate |
| DE602005018907D1 (de) | 2004-03-29 | 2010-03-04 | Inguran Llc | Verwendung einer oxidations-/reduktionsreaktionen intrazellulär und/oder extrazellulär regulierenden zusammensetzung in einem anfärbe- oder sortierungsverfahren für spermatozoen |
| DK2801363T3 (da) | 2004-03-29 | 2018-05-28 | Inguran Llc | Fremgangsmåde til opbevaring af sorterede spermatozoer |
| CA2574499C (en) | 2004-07-22 | 2016-11-29 | Monsanto Technology Llc | Process for enriching a population of sperm cells |
| DK1771729T3 (da) | 2004-07-27 | 2015-11-23 | Beckman Coulter Inc | Forbedring af flowcytometridiskrimination ved hjælp af geometrisk transformation |
| US20060118167A1 (en) | 2004-12-03 | 2006-06-08 | Xy, Inc. | Pressure regulated continuously variable volume container for fluid delivery |
| US20060147894A1 (en) | 2004-12-30 | 2006-07-06 | Vicam, L.P. | Jacketed vessel for holding semen for sex biasing mammals through artificial insemination and systems and methods for enhancing the probability of sex biasing using the same |
| US7591064B2 (en) | 2005-07-20 | 2009-09-22 | Hitachi Global Storage Technologies Netherlands B.V. | Method of fabrication for tape medium read head with unitary formation of multiple elements |
| US7618770B2 (en) | 2005-07-29 | 2009-11-17 | Xy, Inc. | Methods and apparatus for reducing protein content in sperm cell extenders |
| CA2626518A1 (en) | 2005-10-19 | 2007-04-26 | Scott Josephson | Improved reproductive management |
-
2005
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