DK3688189T3 - Fejldetektering under hybridisering af dobbeltstrenget målnukleinsyre - Google Patents

Fejldetektering under hybridisering af dobbeltstrenget målnukleinsyre Download PDF

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Publication number
DK3688189T3
DK3688189T3 DK18782786.0T DK18782786T DK3688189T3 DK 3688189 T3 DK3688189 T3 DK 3688189T3 DK 18782786 T DK18782786 T DK 18782786T DK 3688189 T3 DK3688189 T3 DK 3688189T3
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Denmark
Prior art keywords
nucleic acid
target nucleic
error detection
acid hybridization
stranded target
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DK18782786.0T
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English (en)
Inventor
Matthew James Hayes
Raquel Maria Sanches-Kuiper
Daniel Adrian Bygrave
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Evonetix Ltd
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Publication of DK3688189T3 publication Critical patent/DK3688189T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1031Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/107Temperature of melting, i.e. Tm
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2545/00Reactions characterised by their quantitative nature
    • C12Q2545/10Reactions characterised by their quantitative nature the purpose being quantitative analysis
    • C12Q2545/107Reactions characterised by their quantitative nature the purpose being quantitative analysis with a competitive internal standard/control

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK18782786.0T 2017-09-29 2018-09-27 Fejldetektering under hybridisering af dobbeltstrenget målnukleinsyre DK3688189T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1715852.8A GB2566986A (en) 2017-09-29 2017-09-29 Error detection during hybridisation of target double-stranded nucleic acid
GB1721441.2A GB2568974A (en) 2017-09-29 2017-12-20 Error detection during hybridisation of target double-stranded nucleic acid
PCT/GB2018/052753 WO2019064006A1 (en) 2017-09-29 2018-09-27 ERROR DETECTION DURING TARGET DOUBLE-BIT NUCLEIC ACID HYBRIDIZATION

Publications (1)

Publication Number Publication Date
DK3688189T3 true DK3688189T3 (da) 2022-07-11

Family

ID=60270222

Family Applications (1)

Application Number Title Priority Date Filing Date
DK18782786.0T DK3688189T3 (da) 2017-09-29 2018-09-27 Fejldetektering under hybridisering af dobbeltstrenget målnukleinsyre

Country Status (13)

Country Link
US (1) US11629377B2 (da)
EP (1) EP3688189B1 (da)
JP (1) JP7201674B2 (da)
KR (1) KR102888533B1 (da)
CN (1) CN111164220B (da)
AU (1) AU2018343116B2 (da)
CA (1) CA3077106A1 (da)
DK (1) DK3688189T3 (da)
ES (1) ES2920355T3 (da)
GB (2) GB2566986A (da)
IL (1) IL273298B2 (da)
SG (1) SG11202002188YA (da)
WO (1) WO2019064006A1 (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
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CN111143235A (zh) * 2018-11-06 2020-05-12 爱思开海力士有限公司 多内核存储器系统中的逻辑地址分配
WO2022261366A1 (en) * 2021-06-09 2022-12-15 Avery Digital Data, Inc. Electronic devices for polymer synthesis and assembly
GB2621385A (en) * 2022-08-11 2024-02-14 Evonetix Ltd Reaction duration control for reaction performed on biomolecule samples
GB202300626D0 (en) 2023-01-16 2023-03-01 Evonetix Ltd Hybridisation detection

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Also Published As

Publication number Publication date
US20200248254A1 (en) 2020-08-06
AU2018343116A1 (en) 2020-03-26
SG11202002188YA (en) 2020-04-29
WO2019064006A1 (en) 2019-04-04
JP2020537511A (ja) 2020-12-24
US11629377B2 (en) 2023-04-18
GB2568974A (en) 2019-06-05
GB201715852D0 (en) 2017-11-15
ES2920355T3 (es) 2022-08-03
EP3688189B1 (en) 2022-05-04
GB2566986A (en) 2019-04-03
JP7201674B2 (ja) 2023-01-10
IL273298B2 (en) 2024-10-01
AU2018343116B2 (en) 2024-07-04
IL273298B1 (en) 2024-06-01
GB201721441D0 (en) 2018-01-31
EP3688189A1 (en) 2020-08-05
KR102888533B1 (ko) 2025-11-21
CN111164220B (zh) 2023-12-12
IL273298A (en) 2020-04-30
CA3077106A1 (en) 2019-04-04
CN111164220A (zh) 2020-05-15
KR20200058461A (ko) 2020-05-27

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