DK496489A - Fremgangsmaade til stabilisering af proteiner ved tilrettelaeggelse af indbyrdes elektrostatiske paavirkninger ved metalionbindingssteder - Google Patents

Fremgangsmaade til stabilisering af proteiner ved tilrettelaeggelse af indbyrdes elektrostatiske paavirkninger ved metalionbindingssteder Download PDF

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Publication number
DK496489A
DK496489A DK496489A DK496489A DK496489A DK 496489 A DK496489 A DK 496489A DK 496489 A DK496489 A DK 496489A DK 496489 A DK496489 A DK 496489A DK 496489 A DK496489 A DK 496489A
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DK
Denmark
Prior art keywords
metalion
organization
procedure
electrostatic effects
stabilizing proteins
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DK496489A
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English (en)
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DK496489D0 (da
DK175917B1 (da
Inventor
Michael W Pantoliano
Barry C Finzel
Philip N Bryan
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Genex Corp
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Publication of DK496489D0 publication Critical patent/DK496489D0/da
Publication of DK496489A publication Critical patent/DK496489A/da
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Laminated Bodies (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Detergent Compositions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK198904964A 1987-04-06 1989-10-06 Subtilisinvarianter med foröget termostabilitet DK175917B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US3496587A 1987-04-06 1987-04-06
US3496587 1987-04-06
US8801235 1988-04-06
PCT/US1988/001235 WO1988008028A1 (en) 1987-04-06 1988-04-06 The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins

Publications (3)

Publication Number Publication Date
DK496489D0 DK496489D0 (da) 1989-10-06
DK496489A true DK496489A (da) 1989-10-06
DK175917B1 DK175917B1 (da) 2005-06-27

Family

ID=21879773

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198904964A DK175917B1 (da) 1987-04-06 1989-10-06 Subtilisinvarianter med foröget termostabilitet

Country Status (6)

Country Link
EP (2) EP0916732B1 (da)
JP (2) JP3155984B2 (da)
AT (2) ATE371029T1 (da)
DE (3) DE3856362T2 (da)
DK (1) DK175917B1 (da)
WO (1) WO1988008028A1 (da)

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DE69133632D1 (de) * 1990-01-04 2010-05-27 Danisco Us Inc Glukose-Isomerasen mit geänderter Substratspezifität
DE69133491T2 (de) * 1990-01-04 2006-07-27 Genencor International, Inc., Palo Alto Glucose-Isomerase mit geändertem pH-Optimum
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US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
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CN1148444C (zh) 1997-08-29 2004-05-05 诺沃奇梅兹有限公司 蛋白酶变体及组合物
KR20010023468A (ko) 1997-08-29 2001-03-26 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 프로테아제 변이체 및 조성물
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ATE408678T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
DE60040149D1 (de) 1999-05-20 2008-10-16 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
JP2003505067A (ja) 1999-07-22 2003-02-12 ザ、プロクター、エンド、ギャンブル、カンパニー 特定のエピトープ領域にアミノ酸欠失及び置換を有するサブチリシンプロテアーゼの変異体
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JP7425459B2 (ja) * 2019-10-11 2024-01-31 株式会社シノテスト 安定化されたｈｍｇｂ１含有溶液
JP2023539234A (ja) 2020-08-24 2023-09-13 ノボザイムス アクティーゼルスカブ フルクタナーゼを含む口腔ケア組成物

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AU618675B2 (en) * 1989-05-17 1992-01-02 Amgen, Inc. Multiply mutated subtilisins

Also Published As

Publication number Publication date
EP0353250A4 (en) 1990-09-05
JP3155984B2 (ja) 2001-04-16
EP0916732A1 (en) 1999-05-19
EP0916732B1 (en) 2007-08-22
DK496489D0 (da) 1989-10-06
ATE184316T1 (de) 1999-09-15
DE3856593D1 (de) 2007-10-04
EP0353250A1 (en) 1990-02-07
DK175917B1 (da) 2005-06-27
WO1988008028A1 (en) 1988-10-20
DE3856362D1 (de) 1999-10-14
EP0353250B1 (en) 1999-09-08
ATE371029T1 (de) 2007-09-15
JPH02502874A (ja) 1990-09-13
JP2001029076A (ja) 2001-02-06
DE3856362T2 (de) 2000-05-18
DE353250T1 (de) 1998-05-28

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