ATE184316T1 - Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen - Google Patents

Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen

Info

Publication number
ATE184316T1
ATE184316T1 AT88904113T AT88904113T ATE184316T1 AT E184316 T1 ATE184316 T1 AT E184316T1 AT 88904113 T AT88904113 T AT 88904113T AT 88904113 T AT88904113 T AT 88904113T AT E184316 T1 ATE184316 T1 AT E184316T1
Authority
AT
Austria
Prior art keywords
metal ion
regulation
ion binding
binding sites
electrostatic interactions
Prior art date
Application number
AT88904113T
Other languages
English (en)
Inventor
Michael W Pantoliano
Barry C Finzel
Philip N Bryan
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21879773&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE184316(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE184316T1 publication Critical patent/ATE184316T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Laminated Bodies (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Detergent Compositions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT88904113T 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen ATE184316T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US3496587A 1987-04-06 1987-04-06

Publications (1)

Publication Number Publication Date
ATE184316T1 true ATE184316T1 (de) 1999-09-15

Family

ID=21879773

Family Applications (2)

Application Number Title Priority Date Filing Date
AT98610039T ATE371029T1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen
AT88904113T ATE184316T1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT98610039T ATE371029T1 (de) 1987-04-06 1988-04-06 Regelung von elektrostatischen interaktionen an metallionen-bindungsstellen zur stabilisierung von proteinen

Country Status (6)

Country Link
EP (2) EP0916732B1 (de)
JP (2) JP3155984B2 (de)
AT (2) ATE371029T1 (de)
DE (3) DE3856362T2 (de)
DK (1) DK175917B1 (de)
WO (1) WO1988008028A1 (de)

Families Citing this family (81)

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US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
AU618675B2 (en) * 1989-05-17 1992-01-02 Amgen, Inc. Multiply mutated subtilisins
DE69034159T2 (de) * 1989-06-26 2005-02-17 Unilever N.V. Enzymatische Waschmittelzusammensetzungen
US5665587A (en) 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
BR9006832A (pt) * 1989-06-26 1991-08-06 Unilever Nv Composicao detergente enzimatica
US5115102A (en) * 1989-07-21 1992-05-19 Monsanto Company Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
DE69133632D1 (de) * 1990-01-04 2010-05-27 Danisco Us Inc Glukose-Isomerasen mit geänderter Substratspezifität
DE69133491T2 (de) * 1990-01-04 2006-07-27 Genencor International, Inc., Palo Alto Glucose-Isomerase mit geändertem pH-Optimum
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
ATE245185T1 (de) * 1993-10-14 2003-08-15 Procter & Gamble Proteasen enthaltende bleichmittelzusammensetzungen
HU219851B (hu) * 1993-10-14 2001-08-28 The Procter And Gamble Company Proteáz tartalmú tisztítókészítmények
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
EP0687733A1 (de) * 1994-06-16 1995-12-20 The Procter & Gamble Company Detergenzusammensetzung die Wolle-kompatible-hochalkalische Proteasen enthält
US5922082A (en) 1994-06-16 1999-07-13 Procter & Gamble Company Detergent composition containing wool compatible high alkaline proteases
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
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CN1148444C (zh) 1997-08-29 2004-05-05 诺沃奇梅兹有限公司 蛋白酶变体及组合物
KR20010023468A (ko) 1997-08-29 2001-03-26 한센 핀 베네드, 안네 제헤르, 웨이콥 마리안느 프로테아제 변이체 및 조성물
US6773907B2 (en) 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
AU1225099A (en) 1997-11-21 1999-06-15 Novo Nordisk A/S Protease variants and compositions
US6780629B2 (en) 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
BR9909130A (pt) 1998-03-26 2000-12-19 Procter & Gamble Variantes de protease de serina tendo substituições de amino-ácido
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
EP1082443A1 (de) * 1998-03-26 2001-03-14 The Procter & Gamble Company Serinproteasevarianten mit deletierten und substituierten aminosäuren
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EP1183342B2 (de) 1999-05-20 2012-06-13 Novozymes A/S Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 128 und 129
AU4393100A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 125 and 126
DE60040282D1 (de) 1999-05-20 2008-10-30 Novozymes As SUBTILASE ENZYME DER I-S1 UND I-S2 UNTERGRUPPEN MIT MINDESTENS EINER ZUSäTZLICHEN AMINOSäURE ZWISCHEN POSITION 132 UND 133
ATE408678T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
DE60040149D1 (de) 1999-05-20 2008-10-16 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 126 und 127
JP2003505067A (ja) 1999-07-22 2003-02-12 ザ、プロクター、エンド、ギャンブル、カンパニー 特定のエピトープ領域にアミノ酸欠失及び置換を有するサブチリシンプロテアーゼの変異体
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
WO2001007578A2 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
EP1644471A2 (de) * 1999-10-14 2006-04-12 Novozymes A/S Waschmittelzusammensetzungen mit verbesserter waschleistung enthaltend eine subtilase-variante und ein tensid
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
GB0013643D0 (en) * 2000-05-31 2000-07-26 Unilever Plc Targeted moieties for use in bleach catalysts
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DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
JP4819670B2 (ja) 2003-05-07 2011-11-24 ノボザイムス アクティーゼルスカブ 変異体スブチリシン酵素（サブチラーゼ）
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CN118910005A (zh) 2017-09-27 2024-11-08 诺维信公司 脂肪酶变体和包含此类脂肪酶变体的微囊组合物
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WO2020099491A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising a polypeptide having dnase activity
EP3994255A1 (de) 2019-07-02 2022-05-11 Novozymes A/S Lipasevarianten und zusammensetzungen davon
JP7425459B2 (ja) * 2019-10-11 2024-01-31 株式会社シノテスト 安定化されたｈｍｇｂ１含有溶液
JP2023539234A (ja) 2020-08-24 2023-09-13 ノボザイムス アクティーゼルスカブ フルクタナーゼを含む口腔ケア組成物

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IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
JPS63502959A (ja) * 1986-02-12 1988-11-02 ジェネックス、コ−ポレ−ション 突然変異誘発及びスクリ−ニング方法並びに生成物
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US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
AU618675B2 (en) * 1989-05-17 1992-01-02 Amgen, Inc. Multiply mutated subtilisins

Also Published As

Publication number Publication date
EP0353250A4 (en) 1990-09-05
JP3155984B2 (ja) 2001-04-16
EP0916732A1 (de) 1999-05-19
EP0916732B1 (de) 2007-08-22
DK496489D0 (da) 1989-10-06
DK496489A (da) 1989-10-06
DE3856593D1 (de) 2007-10-04
EP0353250A1 (de) 1990-02-07
DK175917B1 (da) 2005-06-27
WO1988008028A1 (en) 1988-10-20
DE3856362D1 (de) 1999-10-14
EP0353250B1 (de) 1999-09-08
ATE371029T1 (de) 2007-09-15
JPH02502874A (ja) 1990-09-13
JP2001029076A (ja) 2001-02-06
DE3856362T2 (de) 2000-05-18
DE353250T1 (de) 1998-05-28

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