DK496489D0 - Fremgangsmaade til stabilisering af proteiner ved tilrettelaeggelse af indbyrdes elektrostatiske paavirkninger ved metalionbindingssteder - Google Patents

Fremgangsmaade til stabilisering af proteiner ved tilrettelaeggelse af indbyrdes elektrostatiske paavirkninger ved metalionbindingssteder

Info

Publication number
DK496489D0
DK496489D0 DK496489A DK496489A DK496489D0 DK 496489 D0 DK496489 D0 DK 496489D0 DK 496489 A DK496489 A DK 496489A DK 496489 A DK496489 A DK 496489A DK 496489 D0 DK496489 D0 DK 496489D0
Authority
DK
Denmark
Prior art keywords
metalion
organization
procedure
electrostatic effects
stabilizing proteins
Prior art date
Application number
DK496489A
Other languages
English (en)
Other versions
DK175917B1 (da
DK496489A (da
Inventor
Michael W Pantoliano
Barry C Finzel
Philip N Bryan
Original Assignee
Genex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21879773&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK496489(D0) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genex Corp filed Critical Genex Corp
Publication of DK496489D0 publication Critical patent/DK496489D0/da
Publication of DK496489A publication Critical patent/DK496489A/da
Application granted granted Critical
Publication of DK175917B1 publication Critical patent/DK175917B1/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Laminated Bodies (AREA)
  • Manufacture Of Macromolecular Shaped Articles (AREA)
  • Adhesives Or Adhesive Processes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
DK198904964A 1987-04-06 1989-10-06 Subtilisinvarianter med foröget termostabilitet DK175917B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US3496587A 1987-04-06 1987-04-06
US3496587 1987-04-06
PCT/US1988/001235 WO1988008028A1 (en) 1987-04-06 1988-04-06 The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US8801235 1988-04-06

Publications (3)

Publication Number Publication Date
DK496489D0 true DK496489D0 (da) 1989-10-06
DK496489A DK496489A (da) 1989-10-06
DK175917B1 DK175917B1 (da) 2005-06-27

Family

ID=21879773

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198904964A DK175917B1 (da) 1987-04-06 1989-10-06 Subtilisinvarianter med foröget termostabilitet

Country Status (6)

Country Link
EP (2) EP0916732B1 (da)
JP (2) JP3155984B2 (da)
AT (2) ATE371029T1 (da)
DE (3) DE3856593D1 (da)
DK (1) DK175917B1 (da)
WO (1) WO1988008028A1 (da)

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US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
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US5115102A (en) * 1989-07-21 1992-05-19 Monsanto Company Variant proteins and polypeptides possessing enhanced affinity for immobilized-metal affinity matrices
US6271012B1 (en) * 1989-10-11 2001-08-07 Genencor International, Inc. Protease muteins and their use in detergents
DE69133491T2 (de) * 1990-01-04 2006-07-27 Genencor International, Inc., Palo Alto Glucose-Isomerase mit geändertem pH-Optimum
EP0440273B1 (en) * 1990-01-04 2003-04-09 Genencor International Inc. A method for obtaining glucose isomerases having altered substrate specificity
US5340735A (en) 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
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DE69434962T2 (de) * 1993-10-14 2008-01-17 The Procter & Gamble Company, Cincinnati Proteasehaltige reinigungsmittel
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JP4044143B2 (ja) 1996-11-04 2008-02-06 ノボザイムス アクティーゼルスカブ ズブチラーゼ変異体及び組成物
BR9811248B1 (pt) 1997-08-29 2011-10-04 variante de enzima subtilase derivada de uma subtilase originária selecionada a partir do sub-grupo i-s1 ou do sub-grupo i-s2, dita variante tendo melhorado desempenho de lavagem em detergentes em comparação com a subtilase originária, sequência de dna isolada, vetor de expressão, célula hospedeira microbiana, processo para produzir uma variante, composição, uso de uma variante de subtilase.
JP2001514846A (ja) 1997-08-29 2001-09-18 ノボ ノルディスク アクティーゼルスカブ プロテアーゼ変異体及び組成物
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US6569663B1 (en) 1998-03-26 2003-05-27 The Procter & Gamble Company Serine protease variants having amino acid substitutions
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ATE402996T1 (de) 1999-05-20 2008-08-15 Novozymes As Subtilase-enzyme der i-s1 und i-s2 untergruppen mit wenigstens einem zusätzlichen aminosäurerest zwischen positionen 125 und 126
AU4393000A (en) 1999-05-20 2000-12-12 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additionalamino acid residue between positions 128 and 129
WO2000071688A1 (en) 1999-05-20 2000-11-30 Novozymes A/S Subtilase enzymes of the i-s1 and i-s2 sub-groups having at least one additional amino acid residue between positions 126 and 127
ATE408676T1 (de) 1999-05-20 2008-10-15 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 132 und 133
DE60040284D1 (de) 1999-05-20 2008-10-30 Novozymes As Subtilase enzyme der i-s1 und i-s2 untergruppen mit mindestens einer zusätzlichen aminosäure zwischen position 129 und 130
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
CN1373802A (zh) 1999-07-22 2002-10-09 宝洁公司 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体
BR0012660A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
AU7507900A (en) * 1999-10-14 2001-04-23 Novozymes A/S Detergent compositions having improved wash performance comprising a subtilase variant and a surfactant
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
GB0013643D0 (en) * 2000-05-31 2000-07-26 Unilever Plc Targeted moieties for use in bleach catalysts
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
JP4819670B2 (ja) 2003-05-07 2011-11-24 ノボザイムス アクティーゼルスカブ 変異体スブチリシン酵素（サブチラーゼ）
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DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
CN102250861A (zh) * 2004-02-13 2011-11-23 诺维信公司 蛋白酶变体
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US20090215663A1 (en) 2006-04-20 2009-08-27 Novozymes A/S Savinase variants having an improved wash performance on egg stains
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DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
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CN105189724A (zh) 2013-03-14 2015-12-23 诺维信公司 含有酶和抑制剂的水溶性膜
WO2014177709A1 (en) 2013-05-03 2014-11-06 Novozymes A/S Microencapsulation of detergent enzymes
EP3397061A1 (en) 2015-12-28 2018-11-07 Novozymes BioAG A/S Heat priming of bacterial spores
EP4567094A3 (en) 2017-09-27 2026-01-07 Novozymes A/S Lipase variants and microcapsule compositions comprising such lipase variants
WO2019154951A1 (en) 2018-02-08 2019-08-15 Novozymes A/S Lipases, lipase variants and compositions thereof
EP3749759A1 (en) 2018-02-08 2020-12-16 Novozymes A/S Lipase variants and compositions thereof
US20210002588A1 (en) 2018-03-13 2021-01-07 Novozymes A/S Microencapsulation Using Amino Sugar Oligomers
WO2020099491A1 (en) 2018-11-14 2020-05-22 Novozymes A/S Oral care composition comprising a polypeptide having dnase activity
EP3994255A1 (en) 2019-07-02 2022-05-11 Novozymes A/S Lipase variants and compositions thereof
JP7425459B2 (ja) * 2019-10-11 2024-01-31 株式会社シノテスト 安定化されたｈｍｇｂ１含有溶液
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JPS63502959A (ja) * 1986-02-12 1988-11-02 ジェネックス、コ−ポレ−ション 突然変異誘発及びスクリ−ニング方法並びに生成物
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
KR100188532B1 (ko) * 1989-05-17 1999-06-01 스티븐 엠. 오드리 돌연변이 섭티리신 및 그를 함유하는 조성물

Also Published As

Publication number Publication date
ATE184316T1 (de) 1999-09-15
DE3856593D1 (de) 2007-10-04
JP2001029076A (ja) 2001-02-06
EP0353250A1 (en) 1990-02-07
EP0353250A4 (en) 1990-09-05
EP0916732A1 (en) 1999-05-19
DK175917B1 (da) 2005-06-27
EP0916732B1 (en) 2007-08-22
WO1988008028A1 (en) 1988-10-20
DE3856362D1 (de) 1999-10-14
JP3155984B2 (ja) 2001-04-16
DE353250T1 (de) 1998-05-28
DE3856362T2 (de) 2000-05-18
JPH02502874A (ja) 1990-09-13
ATE371029T1 (de) 2007-09-15
EP0353250B1 (en) 1999-09-08
DK496489A (da) 1989-10-06

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