ES2010299A6 - Un procedimiento para la preparacion de analogos de insulina humana que presentan actividad biologica. - Google Patents

Un procedimiento para la preparacion de analogos de insulina humana que presentan actividad biologica.

Info

Publication number
ES2010299A6
ES2010299A6 ES888802408A ES8802408A ES2010299A6 ES 2010299 A6 ES2010299 A6 ES 2010299A6 ES 888802408 A ES888802408 A ES 888802408A ES 8802408 A ES8802408 A ES 8802408A ES 2010299 A6 ES2010299 A6 ES 2010299A6
Authority
ES
Spain
Prior art keywords
dna
circular
sequence
insulin
isolate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES888802408A
Other languages
English (en)
Inventor
Finn Benned Hansen
Per Balschmidt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nordisk Gentofte AS
Original Assignee
Nordisk Gentofte AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nordisk Gentofte AS filed Critical Nordisk Gentofte AS
Publication of ES2010299A6 publication Critical patent/ES2010299A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Obesity (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Emergency Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Hematology (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

UN PROCEDIMIENTO PARA LA PREPARACION DE ANALOGOS DE INSULINA HUMANA QUE PRESENTAN ACTIVIDAD BIOLOGICA. COMPRENDE: INSERTAR LA SECUENCIA DEL PRECURSOR DE INSULINA AISLADA DEL PLASMIDO DE EXPRESION EN UN VECTOR DEL BACTERIOFAGO M13 CIRCULAR MONOFILAMENTAR; FUSIONAR AL GENOMA MONOFILAMENTAR UNA HEBRA DE ADN COMPLEMENTARIA CICLADA QUE CONTIENE LA SECUENCIA DESEADA RODEADA DE SECUENCIAS COMPLETAMENTE HOMOLOGAS A LAS SECUENCIAS DE INSULINA EN EL ADN CIRCULAR; PROLONGAR IN VITRO EL CEBADOR SOBRE LA LONGITUD COMPLETA DEL GENOMA CIRCULAR BIOQUIMICAMENTE UTILIZANDO POLIMERASA KLENOW, OBTENIENDOSE FAGOS MONOFILAMENTARES QUE AL CRECER EN E.COLI PERMITEN AISLAR ADN DE DOBLE HEBRA CON LA SECUENCIA DESEADA; AISLAR UN FRAGMENTO DE RESTRICCION DE ESTE ADN Y REINSERTARLO EN EL VECTOR DE EXPRESION; TRANSFORMARLO EN UN HUESPED, CULTIVAR Y AISLAR EL PRECURSOR DE INSULINA DEL MEDIO DE CULTIVO. ESTOS PRODUCTOS TIENEN UTILIDAD EN EL TRATAMIENTO DE LA DIABETES MELLITUS.
ES888802408A 1988-07-20 1988-08-01 Un procedimiento para la preparacion de analogos de insulina humana que presentan actividad biologica. Expired ES2010299A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/DK1988/000124 WO1990001038A1 (en) 1988-07-20 1988-07-20 Human insulin analogs and preparations containing them

Publications (1)

Publication Number Publication Date
ES2010299A6 true ES2010299A6 (es) 1989-11-01

Family

ID=8153493

Family Applications (1)

Application Number Title Priority Date Filing Date
ES888802408A Expired ES2010299A6 (es) 1988-07-20 1988-08-01 Un procedimiento para la preparacion de analogos de insulina humana que presentan actividad biologica.

Country Status (10)

Country Link
US (1) US5149777A (es)
EP (1) EP0425482B1 (es)
JP (1) JPH03506023A (es)
KR (1) KR900701842A (es)
AT (1) ATE93238T1 (es)
AU (1) AU616205B2 (es)
ES (1) ES2010299A6 (es)
FI (1) FI910286A0 (es)
NO (1) NO905472L (es)
WO (1) WO1990001038A1 (es)

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DK336188D0 (da) * 1988-06-20 1988-06-20 Nordisk Gentofte Propeptider
IL94718A (en) * 1989-07-05 1994-10-21 Schering Ag Carboxamide non-ionic contrast media and method for the preparation thereof
DK10191D0 (da) * 1991-01-22 1991-01-22 Novo Nordisk As Hidtil ukendte peptider
DK33591D0 (es) * 1991-02-27 1991-02-27 Novo Nordisk As
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
DK72793D0 (da) * 1993-06-21 1993-06-21 Novo Nordisk As Nyt produkt
WO1995016708A1 (en) * 1993-12-17 1995-06-22 Novo Nordisk A/S Proinsulin-like compounds
US6001604A (en) * 1993-12-29 1999-12-14 Bio-Technology General Corp. Refolding of proinsulins without addition of reducing agents
US5461031A (en) * 1994-06-16 1995-10-24 Eli Lilly And Company Monomeric insulin analog formulations
US5504188A (en) * 1994-06-16 1996-04-02 Eli Lilly And Company Preparation of stable zinc insulin analog crystals
US5474978A (en) * 1994-06-16 1995-12-12 Eli Lilly And Company Insulin analog formulations
US5547929A (en) * 1994-09-12 1996-08-20 Eli Lilly And Company Insulin analog formulations
US5597893A (en) * 1994-10-31 1997-01-28 Eli Lilly And Company Preparation of stable insulin analog crystals
US5948751A (en) * 1996-06-20 1999-09-07 Novo Nordisk A/S X14-mannitol
JP2001521006A (ja) 1997-10-24 2001-11-06 イーライ・リリー・アンド・カンパニー 不溶性インシュリン組成物
US6531448B1 (en) 1997-12-23 2003-03-11 Eli Lilly And Company Insoluble compositions for controlling blood glucose
AUPP609198A0 (en) * 1998-09-22 1998-10-15 Curtin University Of Technology Use of non-peptidyl compounds for the treatment of insulin related ailments
US6933272B1 (en) 1998-09-22 2005-08-23 Erik Helmerhorst Use of non-peptidyl compounds for the treatment of insulin related ailments
WO2001000223A2 (en) * 1999-06-25 2001-01-04 Minimed Inc. Multiple agent diabetes therapy
WO2002067969A2 (en) * 2001-02-21 2002-09-06 Medtronic Minimed, Inc. Stabilized insulin formulations
US6737401B2 (en) 2001-06-28 2004-05-18 Metronic Minimed, Inc. Methods of evaluating protein formulation stability and surfactant-stabilized insulin formulations derived therefrom
ES2642214T3 (es) 2004-01-21 2017-11-15 Novo Nordisk Health Care Ag Conjugación de péptidos mediante transglutaminasa
US7833513B2 (en) 2004-12-03 2010-11-16 Rhode Island Hospital Treatment of Alzheimer's Disease
CN102660614A (zh) 2005-08-16 2012-09-12 诺沃-诺迪斯克有限公司 制备成熟胰岛素多肽的方法
WO2007081824A2 (en) * 2006-01-06 2007-07-19 Case Western Reserve University Fibrillation resistant proteins
CN101573133B (zh) 2006-07-31 2014-08-27 诺沃-诺迪斯克有限公司 Peg化延长的胰岛素
RU2524150C2 (ru) 2006-09-22 2014-07-27 Ново Нордиск А/С Аналоги инсулина, устойчивые к протеазам
EP2069502B1 (en) 2006-09-27 2014-02-26 Novo Nordisk A/S Method for making maturated insulin polypeptides
WO2008043033A2 (en) 2006-10-04 2008-04-10 Case Western Reserve University Fibrillation-resistant insulin and insulin analogues
JP5496082B2 (ja) 2007-04-30 2014-05-21 ノボ・ノルデイスク・エー／エス タンパク質組成物を乾燥させる方法、乾燥タンパク質組成物、及び乾燥タンパク質を含有する薬学的組成物
EP2017288A1 (en) * 2007-07-16 2009-01-21 Novo Nordisk A/S Protease stabilized, pegylated insulin analogues
JP5688969B2 (ja) * 2007-07-16 2015-03-25 ノボ・ノルデイスク・エー／エス プロテアーゼに対して安定しているペグ化インスリンアナログ
WO2009112583A2 (en) * 2008-03-14 2009-09-17 Novo Nordisk A/S Protease-stabilized insulin analogues
HUE032284T2 (en) 2008-03-18 2017-09-28 Novo Nordisk As Protease-stabilized, acylated insulin analogues
US8993516B2 (en) * 2008-04-14 2015-03-31 Case Western Reserve University Meal-time insulin analogues of enhanced stability
EP2296692A4 (en) * 2008-04-22 2012-06-06 Univ Case Western Reserve ISOFORM-SPECIFIC INSULIN ANALOG
US9200053B2 (en) 2008-07-31 2015-12-01 Case Western Reserve University Insulin analogues containing penta-fluoro-Phenylalanine at position B24
MX2011001181A (es) 2008-07-31 2011-04-05 Univ Case Western Reserve Insulina estabilizada con halogeno.
KR20120129875A (ko) * 2008-07-31 2012-11-28 케이스 웨스턴 리저브 유니버시티 염소화 아미노산을 갖는 인슐린 유사체
JP2012511506A (ja) * 2008-12-09 2012-05-24 ノヴォ・ノルディスク・アー／エス 新規インスリンアナログ
MA33248B1 (fr) 2009-04-01 2012-05-02 Genentech Inc Traitement de troubles résistant à l'insuline
US8399407B2 (en) * 2009-09-17 2013-03-19 Case Western Reserve University Non-standard insulin analogues
AU2010327949A1 (en) 2009-12-11 2012-06-28 Case Western Reserve University Insulin analogues with chlorinated amino acids
JP2014509603A (ja) * 2011-03-15 2014-04-21 ノヴォ ノルディスク アー／エス システイン置換を含むヒトインスリン類似体およびヒトインスリン誘導体
KR20150021011A (ko) 2011-10-27 2015-02-27 케이스 웨스턴 리저브 유니버시티 초고도 농축된, 속효성 인슐린 유사체 제형
KR20140104994A (ko) 2011-12-15 2014-08-29 샹하이 헨그루이 파마수티컬 컴퍼니 리미티드 인간 인슐린 유사체 및 이의 아실화 유도체
CN104364260B (zh) 2012-04-11 2017-02-22 诺和诺德股份有限公司 胰岛素制剂
EP2877200B1 (en) 2012-07-17 2019-05-08 Case Western Reserve University O-linked carbohydrate-modified insulin analogues
JP6499184B2 (ja) 2013-10-07 2019-04-10 ノヴォ ノルディスク アー／エス インスリン類似体の新規な誘導体
BR112017004544A2 (pt) 2014-10-06 2018-01-23 Univ Case Western Reserve insulina de cadeia simples, composição farmacêutica e método para tratar diabetes mellitus
EP3260548A1 (en) 2016-06-21 2017-12-27 Enzypep B.V. Enzymatic coupling of (oligo)peptides to the b-chain of an insulin receptor ligand
PE20210857A1 (es) 2016-12-16 2021-05-18 Novo Nordisk As Composiciones farmaceuticas que contienen insulina

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DK113585D0 (da) * 1985-03-12 1985-03-12 Novo Industri As Nye peptider
DK347086D0 (da) * 1986-07-21 1986-07-21 Novo Industri As Novel peptides
DK119785D0 (da) * 1985-03-15 1985-03-15 Nordisk Gentofte Insulinpraeparat
PH25772A (en) * 1985-08-30 1991-10-18 Novo Industri As Insulin analogues, process for their preparation
DK437786D0 (da) * 1986-09-12 1986-09-12 Nordisk Gentofte Insulinprecursorer

Also Published As

Publication number Publication date
AU2263688A (en) 1990-02-19
WO1990001038A1 (en) 1990-02-08
US5149777A (en) 1992-09-22
AU616205B2 (en) 1991-10-24
FI910286A7 (fi) 1991-01-18
KR900701842A (ko) 1990-12-04
NO905472L (no) 1991-03-20
JPH03506023A (ja) 1991-12-26
EP0425482B1 (en) 1993-08-18
EP0425482A1 (en) 1991-05-08
FI910286A0 (fi) 1991-01-18
NO905472D0 (no) 1990-12-19
ATE93238T1 (de) 1993-09-15

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