ES2051890T3 - Procedimiento para aislar la enzima carnitinhidroliasa de cepas pertenecientes a la familia enterobacteriaceae y empleo de la enzima inmobilizada para producir l(-)-carnitina. - Google Patents

Procedimiento para aislar la enzima carnitinhidroliasa de cepas pertenecientes a la familia enterobacteriaceae y empleo de la enzima inmobilizada para producir l(-)-carnitina.

Info

Publication number
ES2051890T3
ES2051890T3 ES88830425T ES88830425T ES2051890T3 ES 2051890 T3 ES2051890 T3 ES 2051890T3 ES 88830425 T ES88830425 T ES 88830425T ES 88830425 T ES88830425 T ES 88830425T ES 2051890 T3 ES2051890 T3 ES 2051890T3
Authority
ES
Spain
Prior art keywords
enzyme
carnitinhidroliasa
carnitina
isolate
procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88830425T
Other languages
English (en)
Inventor
Heinrich Dr Jung
Kirsten Dr Jung
Hans-Peter Prof Dr Sc Kleber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sigma Tau Industrie Farmaceutiche Riunite SpA
Original Assignee
Sigma Tau Industrie Farmaceutiche Riunite SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DD30824287A external-priority patent/DD281919A7/de
Priority claimed from DD30824387A external-priority patent/DD281735A7/de
Application filed by Sigma Tau Industrie Farmaceutiche Riunite SpA filed Critical Sigma Tau Industrie Farmaceutiche Riunite SpA
Application granted granted Critical
Publication of ES2051890T3 publication Critical patent/ES2051890T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/007—Carnitine; Butyrobetaine; Crotonobetaine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE AISLA UNA ENZIMA CARNITINAHIDROLASA A PARTIR DE MICROORGANISMOS QUE PERTENECEN A LA FAMILIA DE LAS ENTEROBACTERIACEAE, EN PARTICULAR ESCHERICHIA COLI, PROTEUS VULGARIS, PROTEUS MIRABILIS, CITROBACTER FREUNDII, SALMONELLA TYPHIMURIUM, SALMONELLA ANATUM Y SALMONELLA COTTBUS, DESPUES DEL CULTIVO ANAEROBICO DE LOS MICROORGANISMOS EN PRESENCIA DE D, L CARNITINA Y/O CROTOMBETAINA POR DISRUPCION CELULAR Y CROMATOGRAFIA DEL ESTRACTO PROTEICO RESULTANTE. LA ENZIMA POR INMOVILIZACION Y ACTIVACION MEDIANTE UN EFECTOR QUE SE OBTIENE TAMBIEN DEL ESTRACTO PROTEINICO DESPUES DEL AISLAMIENTO DE LA ENZIMA, SE USA PARA HIDRATAR ESTEREOSELECTIVAMENTE CROTOMBETAINA A L(-) - CARNITINA.
ES88830425T 1987-10-26 1988-10-19 Procedimiento para aislar la enzima carnitinhidroliasa de cepas pertenecientes a la familia enterobacteriaceae y empleo de la enzima inmobilizada para producir l(-)-carnitina. Expired - Lifetime ES2051890T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DD30824287A DD281919A7 (de) 1987-10-26 1987-10-26 Verfahren zur herstellung eines enzyms
DD30824387A DD281735A7 (de) 1987-10-26 1987-10-26 Verfahren zur enzymatischen synthese von l(-)-carnitin

Publications (1)

Publication Number Publication Date
ES2051890T3 true ES2051890T3 (es) 1994-07-01

Family

ID=25748172

Family Applications (1)

Application Number Title Priority Date Filing Date
ES88830425T Expired - Lifetime ES2051890T3 (es) 1987-10-26 1988-10-19 Procedimiento para aislar la enzima carnitinhidroliasa de cepas pertenecientes a la familia enterobacteriaceae y empleo de la enzima inmobilizada para producir l(-)-carnitina.

Country Status (4)

Country Link
US (1) US4906568A (es)
EP (1) EP0320460B1 (es)
JP (1) JPH01144996A (es)
ES (1) ES2051890T3 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1240833B (it) * 1990-05-14 1993-12-17 Sigma Tau Ind Farmaceuti Procedimento biocatalitico per la produzione di l-(-)- carnitina da crotonilbetaina e ceppi di proteeae per l'uso in tale procedimento
JP2684238B2 (ja) * 1990-10-09 1997-12-03 旭化成工業株式会社 アシルカルニチンエステラーゼ及びその製造法
FR2715167B1 (fr) * 1994-01-20 1996-04-12 Inst Nat Sciences Appliq Lyon Procédé de production de la L-carnitine par voie microbiologique.
DE19749480A1 (de) * 1997-11-08 1999-05-20 Univ Leipzig Verfahren zur Herstellung von L-Carnitin aus Crotonobetain
DE19850433A1 (de) * 1998-10-27 2000-05-25 Sigma Tau Ind Farmaceuti Crotonobetainyl-Coenzym A, Verfahren zu seiner Herstellung
JP2002528468A (ja) 1998-10-27 2002-09-03 シグマ−タウ・インドゥストリエ・ファルマチェウチケ・リウニテ・ソシエタ・ペル・アチオニ Ｌ−カルニチンの合成に有用な補酵素
US6337197B2 (en) * 1998-10-27 2002-01-08 Sigma-Tau Industrie Farmaceutiche Riunite S.P.A. Coenzymes useful for the synthesis of L-carnitine

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59192095A (ja) * 1983-04-13 1984-10-31 Ajinomoto Co Inc Ｌ−カルニチンの製造法
DD221905B1 (de) * 1983-11-03 1987-03-18 Univ Leipzig Verfahren zur herstellung von l(-)-carnitin und seinen derivaten
JPS6167494A (ja) * 1984-09-06 1986-04-07 Seitetsu Kagaku Co Ltd Ｌ−カルニチンの製造方法

Also Published As

Publication number Publication date
EP0320460A3 (en) 1990-05-23
EP0320460B1 (en) 1994-04-27
US4906568A (en) 1990-03-06
JPH01144996A (ja) 1989-06-07
EP0320460A2 (en) 1989-06-14

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