ES2101552T3 - Regulacion de la recombinacion especifica del sitio por proteinas de fusion recombinasa especifica del sitio/receptor nuclear. - Google Patents

Regulacion de la recombinacion especifica del sitio por proteinas de fusion recombinasa especifica del sitio/receptor nuclear.

Info

Publication number
ES2101552T3
ES2101552T3 ES94921628T ES94921628T ES2101552T3 ES 2101552 T3 ES2101552 T3 ES 2101552T3 ES 94921628 T ES94921628 T ES 94921628T ES 94921628 T ES94921628 T ES 94921628T ES 2101552 T3 ES2101552 T3 ES 2101552T3
Authority
ES
Spain
Prior art keywords
site
recombination
recombinase
fusion proteins
regulation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES94921628T
Other languages
English (en)
Inventor
Francis Stewart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Europaisches Laboratorium fuer Molekularbiologie EMBL
Original Assignee
Europaisches Laboratorium fuer Molekularbiologie EMBL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Europaisches Laboratorium fuer Molekularbiologie EMBL filed Critical Europaisches Laboratorium fuer Molekularbiologie EMBL
Application granted granted Critical
Publication of ES2101552T3 publication Critical patent/ES2101552T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/721—Steroid/thyroid hormone superfamily, e.g. GR, EcR, androgen receptor, oestrogen receptor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

SE DESCRIBE UNA INVENCION QUE PERMITE LA REGULACION DE RECOMBINACION EN CELULAS, ORGANISMOS O SISTEMAS LIBRES DE CELULAS APROPIADOS. LA INVENCION CONSISTE EN CREAR PROTEINAS DE FUSION ENTRE PROTEINA DE RECOMBINASA, O COMPONENTES DE SISTEMA DE RECOMBINASA, Y LIGANDOS QUE ENLAZAN CAMPOS DERIVADOS DE RECEPTORES NUCLEARES. LAS PROTEINAS FUSION MUESTRAN UNA PEQUEÑA ACTIVIDAD DE RECOMBINASA EN AUSENCIA DEL LIGANDO QUE SE ENLAZA AL CAMPO DE ENLACE DE LIGANDO. AL ENLAZAR EL LIGANDO, LA ACTIVIDAD DE RECOMBINASA SE REDUCE.LA INVENCION PROPORCIONA UN MEDIO PRACTICO PARA REGULAR LA RECOMBINACION EN LAS CELULAS Y ORGANISMOS Y, ENLAZANDO ENLACE DE LIGANDO A RECOMBINACION, SE PROPORCIONA UN MEDIO SENCILLO CON LO QUE EL ENLACE DE LIGANDO PUEDE SER MEDIDO COMO RECOMBINACION REALIZADA.
ES94921628T 1993-06-28 1994-06-28 Regulacion de la recombinacion especifica del sitio por proteinas de fusion recombinasa especifica del sitio/receptor nuclear. Expired - Lifetime ES2101552T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP93110298A EP0632054A1 (en) 1993-06-28 1993-06-28 Regulation of site-specific recombination by site-specific recombinase/nuclear receptor fusion proteins

Publications (1)

Publication Number Publication Date
ES2101552T3 true ES2101552T3 (es) 1997-07-01

Family

ID=8213015

Family Applications (1)

Application Number Title Priority Date Filing Date
ES94921628T Expired - Lifetime ES2101552T3 (es) 1993-06-28 1994-06-28 Regulacion de la recombinacion especifica del sitio por proteinas de fusion recombinasa especifica del sitio/receptor nuclear.

Country Status (9)

Country Link
US (1) US6040430A (es)
EP (2) EP0632054A1 (es)
JP (1) JP2986915B2 (es)
AT (1) ATE152123T1 (es)
AU (1) AU7227994A (es)
CA (1) CA2166198C (es)
DE (1) DE69402863T2 (es)
ES (1) ES2101552T3 (es)
WO (1) WO1995000555A1 (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2322614A1 (en) * 1995-06-07 2011-05-18 Life Technologies Corporation Recombinational cloning using engineered recombination sites
US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
FR2749857B1 (fr) * 1996-06-12 1998-08-14 Centre Nat Rech Scient Generation de molecules replicatives in vivo
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
EP1025217B1 (en) * 1997-10-24 2006-10-04 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US7102055B1 (en) 1997-11-18 2006-09-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant
US5929301A (en) * 1997-11-18 1999-07-27 Pioneer Hi-Bred International Nucleic acid sequence encoding FLP recombinase
WO1999025821A1 (en) 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
ES2256972T3 (es) 1997-11-18 2006-07-16 Pioneer Hi-Bred International, Inc. Metodo novedoso para la integracion de un adn foraneo dentro de genomas de eucariotas.
US6720475B1 (en) 1997-11-18 2004-04-13 Pioneer Hi-Bred International, Inc. Modified nucleic acid sequence encoding FLP recombinase
AU745238C (en) 1997-11-18 2003-02-27 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from T-DNA using site-specific recombination systems
EP1173460B1 (en) * 1999-03-02 2009-09-16 Life Technologies Corporation Compositions and methods for use in recombinational cloning of nucleic acids
GB9924604D0 (en) * 1999-10-18 1999-12-22 European Molecular Biology Lab Embl Genetic switch
DE19956272B4 (de) * 1999-11-23 2004-09-16 Mpb Cologne Gmbh Molecular Plant & Protein Biotechnology Verfahren zur gesteuerten Nach-Ernte-Produktion von Proteinen in Wirtsorganismen
NZ539430A (en) 1999-12-10 2006-09-29 Invitrogen Corp Use of multiple recombination sites with unique specificity in recombinational cloning
EP1118668A1 (en) * 2000-01-07 2001-07-25 ARTEMIS Pharmaceuticals GmbH Transduction of recombinases for inducible gene targeting
DE10024740A1 (de) * 2000-05-19 2001-11-29 Mpb Cologne Gmbh Molecular Pla Verfahren zur gesteuerten Eliminierung gewünschter DNA-Sequenzen in Wirtsorganismen
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
DE10038573A1 (de) * 2000-08-03 2002-02-21 Mpb Cologne Gmbh Molecular Pla Verfahren zur Selektion auf Wirtszellen mit eliminierten DNA-Sequenzen
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
FR2814642B1 (fr) 2000-10-03 2005-07-01 Ass Pour Le Dev De La Rech En Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee
EP1322152A2 (en) * 2000-10-03 2003-07-02 ADEREGEM (Association pour le Développement de la Recherche en Génétique Moléculaire Transgenic mouse for targeted recombination mediated by modified cre-er
US7560622B2 (en) 2000-10-06 2009-07-14 Pioneer Hi-Bred International, Inc. Methods and compositions relating to the generation of partially transgenic organisms
JP2004531259A (ja) * 2001-04-19 2004-10-14 インヴィトロジェン コーポレーション 核酸分子の組換えクローニングのための組成物および方法
EP1264891A1 (en) * 2001-05-31 2002-12-11 Plant Research International B.V. Modification of plant genomes by inducible site-specific recombination of transgenes
JP2005521400A (ja) 2002-03-29 2005-07-21 シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト 植物におけるラムダインテグラーゼ媒介型組換え
JP2005532829A (ja) * 2002-07-18 2005-11-04 インヴィトロジェン コーポレーション 組換え部位を含むウイルスベクター
WO2005028615A2 (en) * 2003-06-26 2005-03-31 Invitrogen Corporation Methods and compositions for detecting promoter activity and expressing fusion proteins
WO2005054438A2 (en) * 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
US7935862B2 (en) 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
JP4485475B2 (ja) * 2004-02-12 2010-06-23 独立行政法人科学技術振興機構 核内レセプターのアゴニスト・アンタゴニスト検出用プローブとそれを用いた核内レセプターに対するアゴニストおよびアンタゴニストのスクリーニング方法
WO2008008160A2 (en) * 2006-07-07 2008-01-17 Fred Hutchinson Cancer Research Center High efficiency flp site-specific recombination in mammalian cells using an optimized flp gene
JP6215196B2 (ja) 2011-05-31 2017-10-18 キージーン・エン・フェー 有害生物耐性植物
US20150059018A1 (en) 2011-10-19 2015-02-26 Keygene N.V. Methods and compositions for producing drimenol
WO2014142647A1 (en) 2013-03-14 2014-09-18 Wageningen Universiteit Fungals strains with improved citric acid and itaconic acid production
IL257831B2 (en) 2015-09-04 2025-11-01 Keygene Nv Diplosporic garden
WO2020239984A1 (en) 2019-05-29 2020-12-03 Keygene N.V. Gene for parthenogenesis
IL301700A (en) 2020-10-13 2023-05-01 Keygene Nv Modified promoter of a parthenogenesis gene

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0146050B1 (en) * 1983-12-16 1990-07-04 Denis M. Callewaert Site selective plasminogen activator and method of making and using same
WO1993015191A1 (en) * 1992-01-24 1993-08-05 Life Technologies, Inc. Modulation of enzyme activities in the in vivo cloning of dna
US5589362A (en) * 1993-06-14 1996-12-31 Basf Aktiengesellschaft Tetracycline regulated transcriptional modulators with altered DNA binding specificities

Also Published As

Publication number Publication date
EP0707599A1 (en) 1996-04-24
CA2166198A1 (en) 1995-01-05
WO1995000555A1 (en) 1995-01-05
AU7227994A (en) 1995-01-17
EP0707599B1 (en) 1997-04-23
JPH08511681A (ja) 1996-12-10
DE69402863D1 (de) 1997-05-28
US6040430A (en) 2000-03-21
DE69402863T2 (de) 1997-07-31
EP0632054A1 (en) 1995-01-04
ATE152123T1 (de) 1997-05-15
CA2166198C (en) 2002-01-08
JP2986915B2 (ja) 1999-12-06

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