ES2127697A1 - Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos. - Google Patents

Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

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Publication number
ES2127697A1
ES2127697A1 ES09700482A ES9700482A ES2127697A1 ES 2127697 A1 ES2127697 A1 ES 2127697A1 ES 09700482 A ES09700482 A ES 09700482A ES 9700482 A ES9700482 A ES 9700482A ES 2127697 A1 ES2127697 A1 ES 2127697A1
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Spain
Prior art keywords
promoters
expression
actine
sens
secretion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES09700482A
Other languages
English (en)
Other versions
ES2127697B1 (es
Inventor
Fuente Jose Luis Barredo
Saiz Marta Rodriguez
Valle Miguel Angel Moreno
De La Vieja Alfonso J Collados
Maldonado Francisco Salto
Garcia Bruno Diez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to ES009700482A priority Critical patent/ES2127697B1/es
Application filed by Antibioticos SA filed Critical Antibioticos SA
Priority to KR1019980708912A priority patent/KR20000065202A/ko
Priority to PL98329722A priority patent/PL329722A1/xx
Priority to SK1495-98A priority patent/SK149598A3/sk
Priority to CZ983535A priority patent/CZ353598A3/cs
Priority to ZA981896A priority patent/ZA981896B/xx
Priority to EE9800381A priority patent/EE9800381A/xx
Priority to CN98800237A priority patent/CN1219200A/zh
Priority to IL12664798A priority patent/IL126647A0/xx
Priority to PCT/ES1998/000056 priority patent/WO1998039459A1/es
Priority to JP10538200A priority patent/JP2000509613A/ja
Priority to CA002253250A priority patent/CA2253250A1/en
Priority to HU0000870A priority patent/HUP0000870A3/hu
Priority to US09/171,337 priority patent/US6300095B1/en
Priority to AU61011/98A priority patent/AU6101198A/en
Priority to ES009801858A priority patent/ES2149706B1/es
Priority to ES009801859A priority patent/ES2149707B1/es
Priority to LT98-152A priority patent/LT4557B/lt
Priority to LVP-98-268A priority patent/LV12264B/en
Publication of ES2127697A1 publication Critical patent/ES2127697A1/es
Application granted granted Critical
Publication of ES2127697B1 publication Critical patent/ES2127697B1/es
Priority to US09/631,022 priority patent/US6558921B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Promotores de los genes glutamato deshidrogenasa, {b -N-acetilhexosaminidasa y {y -actina y su utilización en sistemas de expresión, secreción y antisentido de hongos filamentosos. Se describe la utilización de los promotores de los genes que codifican: (I) glutamato deshidrogenasa NADP dependiente (EC.1.4.1.4) de Penicillium chrysogenum, (II) {b -N-acetilhexosaminidasa (EC.3.2.1.52) de Penicillium chrysogenum y (III) {y -actina de Penicillium chrysogenum y Acremonium chrysogenum, los cuales pueden ser utilizados para la construcción de potentes vectores de expresión y secreción útiles tanto para P. chrysogenum como para A. chrysogenum y especies relacionadas. Asimismo, estos promotores pueden utilizarse para el bloqueo de la expresión génica mediante construcciones antisentido. Bajo el control de los promotores anteriormente citados se puede dirigir la expresión de otros genes en hongos filamentosos, incrementándose la producción de antibióticos y/o proteínas inherentes a losimismos.
ES009700482A 1997-03-05 1997-03-05 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos. Expired - Fee Related ES2127697B1 (es)

Priority Applications (20)

Application Number Priority Date Filing Date Title
ES009700482A ES2127697B1 (es) 1997-03-05 1997-03-05 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
CA002253250A CA2253250A1 (en) 1997-03-05 1998-03-05 Promoters of the genes glutamate deshydrogenase, .beta.-n-acetylhexosaminidase and $b(g)-actine, and their use in systems of expression, secretion and anti-sens in filamentary fungi
SK1495-98A SK149598A3 (en) 1997-03-05 1998-03-05 Promoters of the genes glutamate deshydrogenase, 'beta'-n- -acetylhexosaminidase and gamma-actine, and their use in systems of expression, secretion and anti-sens in filamentary fungi
CZ983535A CZ353598A3 (cs) 1997-03-05 1998-03-05 Způsob zvýšení nebo blokování genové exprese pomocí promotorů genů glutamátdehydrogenázy, ß-N-hexózoaminidázy a gama-aktinu a použití takových promotorů pro výrobu antibiotik
ZA981896A ZA981896B (en) 1997-03-05 1998-03-05 Glutamate dehydrogenase beta-n-acetylhexosaminidase and gamma-actin gene promoters and their use in expression secretion and antisense systems of filamentary fungi
EE9800381A EE9800381A (et) 1997-03-05 1998-03-05 Glutamaatdehüdrogenaasi, ß-N-atsetüülheksoosaminidaasi ja y-aktiini geenide promootorid ning nende kasutamine niitseente ekspressiooni-, sekretsiooni- ja antisenss-süsteemides
PL98329722A PL329722A1 (en) 1997-03-05 1998-03-05 Promotors of glutaminic dehydrogenase, beta-n-acetylhexosoaminmidaze and actin genes and their application for expansion, secretion and in antisensory systems of filiform fungi
IL12664798A IL126647A0 (en) 1997-03-05 1998-03-05 Promoters of the genes glutamate dehydrogenase beta-n-acetylhexosiminidase and gamma-actin and their use in systems of expression secretion and anti-sense in filamentary fungi
PCT/ES1998/000056 WO1998039459A1 (es) 1997-03-05 1998-03-05 PROMOTORES DE LOS GENES GLUTAMATO DESHIDROGENASA, β-N-ACETILHEXOSAMINIDASA η-ACTINA Y SU UTILIZACION EN SISTEMAS DE EXPRESION, SECRECION Y ANTISENTIDO DE HONGOS FILAMENTOSOS
JP10538200A JP2000509613A (ja) 1997-03-05 1998-03-05 遺伝子グルタミン酸デヒドロゲナーゼ、β―Ｎ―アセチルヘキソサミニダーゼ及びγ―アクチンのプロモーター並びにそれらの糸状菌発現、分泌及びアンチセンスシステムにおける使用
KR1019980708912A KR20000065202A (ko) 1997-03-05 1998-03-05 유전자 글루탐산 탈수소효소, β­N­아세틸헥소사미니다제,γ­액틴의 프로모터 및 그의 사상균 발현, 분비 및 안티센스시스템에의 사용
CN98800237A CN1219200A (zh) 1997-03-05 1998-03-05 谷氨酸脱氢酶、β－N－乙酰氨基己糖苷酶和γ－肌动蛋白基因的启动子以及它们在丝状真菌表达,分泌和反义系统中的应用
HU0000870A HUP0000870A3 (en) 1997-03-05 1998-03-05 Promoters of the genes glutamate deshydrogenase, betha-n-acetylhexosaminidase and gamma-actine, and their use in systems of expression, secretion and anti-sens in filamentary fungi
AU61011/98A AU6101198A (en) 1997-03-05 1998-03-05 Promoters of the genes glutamate deshydrogenase, beta-N-acetylhexosa minidase and$b(g)-actine, and their use in systems of expression, secretion an d anti-sens in filamentary fungi
US09/171,337 US6300095B1 (en) 1997-03-05 1998-03-05 Promoters of the genes glutamate dehydrogenase β-N-acetylhexosaminidase and γ-actin and their use in filamentous fungi expression, secretion and antisense systems
ES009801859A ES2149707B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosa,omodasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
ES009801858A ES2149706B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidas y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
LT98-152A LT4557B (lt) 1997-03-05 1998-10-23 Glutamato dehidrogenazės, beta-n-acetilheksozaminidazės ir gama-aktino genų promotoriai bei jų panaudojimas filamentinių grybų ekspresijos, sekrecijos ir antisensinėse sistemose
LVP-98-268A LV12264B (en) 1997-03-05 1998-11-05 Promoters of the genses glutamate deshydrogenase, beta-n-acetylhexosaminidase and gamma-actine, and their use in systems of expression, secretion and anti-sens in filamentary fungi
US09/631,022 US6558921B1 (en) 1997-03-05 2000-08-02 Promoters of the genes glutamate deshydrogenase, β-N-acetylhexosaminidase and γ-actin and their use in filamentous fungi expression, secretion and antisense systems

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009700482A ES2127697B1 (es) 1997-03-05 1997-03-05 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Publications (2)

Publication Number Publication Date
ES2127697A1 true ES2127697A1 (es) 1999-04-16
ES2127697B1 ES2127697B1 (es) 2000-03-16

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ID=8298517

Family Applications (3)

Application Number Title Priority Date Filing Date
ES009700482A Expired - Fee Related ES2127697B1 (es) 1997-03-05 1997-03-05 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
ES009801858A Expired - Fee Related ES2149706B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidas y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
ES009801859A Expired - Fee Related ES2149707B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosa,omodasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES009801858A Expired - Fee Related ES2149706B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidas y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
ES009801859A Expired - Fee Related ES2149707B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosa,omodasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Country Status (17)

Country Link
US (2) US6300095B1 (es)
JP (1) JP2000509613A (es)
KR (1) KR20000065202A (es)
CN (1) CN1219200A (es)
AU (1) AU6101198A (es)
CA (1) CA2253250A1 (es)
CZ (1) CZ353598A3 (es)
EE (1) EE9800381A (es)
ES (3) ES2127697B1 (es)
HU (1) HUP0000870A3 (es)
IL (1) IL126647A0 (es)
LT (1) LT4557B (es)
LV (1) LV12264B (es)
PL (1) PL329722A1 (es)
SK (1) SK149598A3 (es)
WO (1) WO1998039459A1 (es)
ZA (1) ZA981896B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6777203B1 (en) * 1997-11-19 2004-08-17 Geron Corporation Telomerase promoter driving expression of therapeutic gene sequences
US6610839B1 (en) 1997-08-14 2003-08-26 Geron Corporation Promoter for telomerase reverse transcriptase
ES2127697B1 (es) * 1997-03-05 2000-03-16 Antibioticos Sau Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
US7378244B2 (en) * 1997-10-01 2008-05-27 Geron Corporation Telomerase promoters sequences for screening telomerase modulators
ES2143408B1 (es) * 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
CA2372285A1 (en) * 1999-03-03 2000-09-08 Marcus Hartmann .beta.-hexosaminidase, dna sequence from ciliates for coding the same and use thereof
EP2009093A3 (en) * 2004-09-22 2009-04-08 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
EP1801221A1 (en) * 2005-12-22 2007-06-27 Sandoz AG Promoter sequences
CN102127546B (zh) * 2010-11-24 2012-10-24 山东农业大学 一种骨骼肌特异性actin启动子及其应用
CN116004892B (zh) * 2022-10-31 2025-04-29 中国热带农业科学院环境与植物保护研究所 一种香蕉灰纹病菌的pcr检测引物及检测方法

Citations (2)

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Publication number Priority date Publication date Assignee Title
EP0215539A2 (en) * 1985-04-04 1987-03-25 Glaxo Group Limited Plasmids and components thereof for use in filamentous fungi
EP0225078A2 (en) * 1985-11-20 1987-06-10 Panlabs, Inc. Composite expression cassettes for fungal transformation

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HUP0000870A2 (en) 2000-07-28
KR20000065202A (ko) 2000-11-06
HUP0000870A3 (en) 2001-09-28
IL126647A0 (en) 1999-08-17
ES2149707B1 (es) 2001-05-16
US6300095B1 (en) 2001-10-09
CZ353598A3 (cs) 1999-02-17
ES2127697B1 (es) 2000-03-16
WO1998039459A1 (es) 1998-09-11
LV12264A (lv) 1999-04-20
EE9800381A (et) 1999-04-15
LT4557B (lt) 1999-10-25
ES2149707A1 (es) 2000-11-01
CA2253250A1 (en) 1998-09-11
US6558921B1 (en) 2003-05-06
ES2149706B1 (es) 2001-05-16
ZA981896B (en) 1998-09-14
AU6101198A (en) 1998-09-22
PL329722A1 (en) 1999-04-12
CN1219200A (zh) 1999-06-09
SK149598A3 (en) 2000-03-13
ES2149706A1 (es) 2000-11-01
LV12264B (en) 1999-10-20
JP2000509613A (ja) 2000-08-02
LT98152A (en) 1999-06-25

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