ES2149707A1 - Promotores de los genes glutamato deshidrogenasa, b-n-acetilhexosaminidasa y y-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos. - Google Patents

Promotores de los genes glutamato deshidrogenasa, b-n-acetilhexosaminidasa y y-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

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Publication number
ES2149707A1
ES2149707A1 ES009801859A ES9801859A ES2149707A1 ES 2149707 A1 ES2149707 A1 ES 2149707A1 ES 009801859 A ES009801859 A ES 009801859A ES 9801859 A ES9801859 A ES 9801859A ES 2149707 A1 ES2149707 A1 ES 2149707A1
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Prior art keywords
promoters
expression
actine
sens
secretion
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Granted
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ES009801859A
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English (en)
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ES2149707B1 (es
Inventor
Fuente Jose Luis Barredo
Saiz Marta Rodriguez
Valle Miguel Angel Moreno
De La Vieja Alfonso J Collados
Maldonado Francisco Salto
Garcia Bruno Diez
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Antibioticos SA
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Antibioticos SA
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Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Promotores de los genes glutamato deshidrogenasa, {b -N-acetilhexosaminidasa y {y -actina y su utilización en sistemas de expresión, secrecion y antisentido de hongos filamentosos. Se describe la utilización de los promotores de los genes que codifican: (1) glutamato deshidrogenasa NADP dependiente (EC.1.4.1.4) de Penicillium chrysogenum, (II) {b -N-acetilhexosa-minidasa (EC.3.2.1.52) de Penicililum chrysogenum y (III) {y -actina de Penicillium chrysogenum y Acremonium chrysogenum, los cuales pueden ser utilizados para la construcción de potentes vectores de expresión y secreción útiles tanto para P. chrysogenum como para A. chrysogenum y especies relacionadas. Asimismo, estos promotores pueden utilizarse para el bloqueo de la expresión génica mediante construcciones antisentido. Bajo el control de los promotores anteriormente citados se puede dirigir la expresiónd e otros genes en hongos filamentosos, incrementándose la producción de antibióticos y/ o proteínas inherentes a los mismos.
ES009801859A 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosa,omodasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos. Expired - Fee Related ES2149707B1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009700482A ES2127697B1 (es) 1997-03-05 1997-03-05 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Publications (2)

Publication Number Publication Date
ES2149707A1 true ES2149707A1 (es) 2000-11-01
ES2149707B1 ES2149707B1 (es) 2001-05-16

Family

ID=8298517

Family Applications (3)

Application Number Title Priority Date Filing Date
ES009700482A Expired - Fee Related ES2127697B1 (es) 1997-03-05 1997-03-05 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
ES009801859A Expired - Fee Related ES2149707B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosa,omodasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
ES009801858A Expired - Fee Related ES2149706B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidas y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES009700482A Expired - Fee Related ES2127697B1 (es) 1997-03-05 1997-03-05 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES009801858A Expired - Fee Related ES2149706B1 (es) 1997-03-05 1998-09-03 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidas y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Country Status (17)

Country Link
US (2) US6300095B1 (es)
JP (1) JP2000509613A (es)
KR (1) KR20000065202A (es)
CN (1) CN1219200A (es)
AU (1) AU6101198A (es)
CA (1) CA2253250A1 (es)
CZ (1) CZ353598A3 (es)
EE (1) EE9800381A (es)
ES (3) ES2127697B1 (es)
HU (1) HUP0000870A3 (es)
IL (1) IL126647A0 (es)
LT (1) LT4557B (es)
LV (1) LV12264B (es)
PL (1) PL329722A1 (es)
SK (1) SK149598A3 (es)
WO (1) WO1998039459A1 (es)
ZA (1) ZA981896B (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6777203B1 (en) 1997-11-19 2004-08-17 Geron Corporation Telomerase promoter driving expression of therapeutic gene sequences
US6610839B1 (en) 1997-08-14 2003-08-26 Geron Corporation Promoter for telomerase reverse transcriptase
ES2127697B1 (es) * 1997-03-05 2000-03-16 Antibioticos Sau Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
US7378244B2 (en) * 1997-10-01 2008-05-27 Geron Corporation Telomerase promoters sequences for screening telomerase modulators
ES2143408B1 (es) * 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
CA2372285A1 (en) * 1999-03-03 2000-09-08 Marcus Hartmann .beta.-hexosaminidase, dna sequence from ciliates for coding the same and use thereof
US20090104165A1 (en) * 2004-09-22 2009-04-23 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
EP1801221A1 (en) * 2005-12-22 2007-06-27 Sandoz AG Promoter sequences
CN102127546B (zh) * 2010-11-24 2012-10-24 山东农业大学 一种骨骼肌特异性actin启动子及其应用
CN116904452A (zh) * 2021-10-28 2023-10-20 冯波 一种启动子及其在提高谷氨酸脱氢酶活性或氨基酸产量、糖酸转化率上的应用
CN116004892B (zh) * 2022-10-31 2025-04-29 中国热带农业科学院环境与植物保护研究所 一种香蕉灰纹病菌的pcr检测引物及检测方法
CN121574936B (zh) * 2025-12-01 2026-07-03 中国食品药品检定研究院 一种快速检测药物过敏和/或类过敏反应的发光细胞及其构建方法和应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0215539A2 (en) * 1985-04-04 1987-03-25 Glaxo Group Limited Plasmids and components thereof for use in filamentous fungi

Family Cites Families (2)

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Publication number Priority date Publication date Assignee Title
FI864473A7 (fi) * 1985-11-20 1987-05-21 Panlabs Inc Yhdistetyt ilmaisukasetit fungaalitransformaatiota varten.
ES2127697B1 (es) * 1997-03-05 2000-03-16 Antibioticos Sau Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0215539A2 (en) * 1985-04-04 1987-03-25 Glaxo Group Limited Plasmids and components thereof for use in filamentous fungi

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FIDEL, S. et al. "Aspergillus nidulans contains a single actin which has unique intron location and encodes a gamma-actin", GENE, 1988, Vol. 70, paginas 283-293. *
FREDERICK, G.D. et al. "Distant upstream regulatory sequences control the level of expression of de am (GDH) locus of Neurospora crassa", CURRENT GENETICS, 1990, Vol. 8, paginas 53-58. *

Also Published As

Publication number Publication date
WO1998039459A1 (es) 1998-09-11
LT98152A (en) 1999-06-25
HUP0000870A3 (en) 2001-09-28
US6558921B1 (en) 2003-05-06
ES2149706B1 (es) 2001-05-16
LT4557B (lt) 1999-10-25
KR20000065202A (ko) 2000-11-06
ZA981896B (en) 1998-09-14
ES2127697A1 (es) 1999-04-16
ES2149706A1 (es) 2000-11-01
HUP0000870A2 (en) 2000-07-28
LV12264B (en) 1999-10-20
JP2000509613A (ja) 2000-08-02
CN1219200A (zh) 1999-06-09
PL329722A1 (en) 1999-04-12
ES2127697B1 (es) 2000-03-16
IL126647A0 (en) 1999-08-17
US6300095B1 (en) 2001-10-09
CA2253250A1 (en) 1998-09-11
EE9800381A (et) 1999-04-15
ES2149707B1 (es) 2001-05-16
CZ353598A3 (cs) 1999-02-17
SK149598A3 (en) 2000-03-13
LV12264A (lv) 1999-04-20
AU6101198A (en) 1998-09-22

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