LT4557B - Glutamato dehidrogenazės, beta-n-acetilheksozaminidazės ir gama-aktino genų promotoriai bei jų panaudojimas filamentinių grybų ekspresijos, sekrecijos ir antisensinėse sistemose - Google Patents

Glutamato dehidrogenazės, beta-n-acetilheksozaminidazės ir gama-aktino genų promotoriai bei jų panaudojimas filamentinių grybų ekspresijos, sekrecijos ir antisensinėse sistemose Download PDF

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Publication number
LT4557B
LT4557B LT98-152A LT98152A LT4557B LT 4557 B LT4557 B LT 4557B LT 98152 A LT98152 A LT 98152A LT 4557 B LT4557 B LT 4557B
Authority
LT
Lithuania
Prior art keywords
chrysogenum
gene
gly
seq
ala
Prior art date
Application number
LT98-152A
Other languages
English (en)
Lithuanian (lt)
Other versions
LT98152A (en
Inventor
Jose Luis Barredo-Fuente
Marta Rodriguez-Saiz
Miguel Angel Moreno-Valle
Alfonso J. Collados-De-La-Vieja
Francisco Salto-Maldonado
Bruno Diez-Garcia
Original Assignee
Antibioticos, S.A.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos, S.A. filed Critical Antibioticos, S.A.
Publication of LT98152A publication Critical patent/LT98152A/xx
Publication of LT4557B publication Critical patent/LT4557B/lt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01002—Glutamate dehydrogenase (1.4.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
LT98-152A 1997-03-05 1998-10-23 Glutamato dehidrogenazės, beta-n-acetilheksozaminidazės ir gama-aktino genų promotoriai bei jų panaudojimas filamentinių grybų ekspresijos, sekrecijos ir antisensinėse sistemose LT4557B (lt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009700482A ES2127697B1 (es) 1997-03-05 1997-03-05 Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Publications (2)

Publication Number Publication Date
LT98152A LT98152A (en) 1999-06-25
LT4557B true LT4557B (lt) 1999-10-25

Family

ID=8298517

Family Applications (1)

Application Number Title Priority Date Filing Date
LT98-152A LT4557B (lt) 1997-03-05 1998-10-23 Glutamato dehidrogenazės, beta-n-acetilheksozaminidazės ir gama-aktino genų promotoriai bei jų panaudojimas filamentinių grybų ekspresijos, sekrecijos ir antisensinėse sistemose

Country Status (17)

Country Link
US (2) US6300095B1 (es)
JP (1) JP2000509613A (es)
KR (1) KR20000065202A (es)
CN (1) CN1219200A (es)
AU (1) AU6101198A (es)
CA (1) CA2253250A1 (es)
CZ (1) CZ353598A3 (es)
EE (1) EE9800381A (es)
ES (3) ES2127697B1 (es)
HU (1) HUP0000870A3 (es)
IL (1) IL126647A0 (es)
LT (1) LT4557B (es)
LV (1) LV12264B (es)
PL (1) PL329722A1 (es)
SK (1) SK149598A3 (es)
WO (1) WO1998039459A1 (es)
ZA (1) ZA981896B (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6610839B1 (en) * 1997-08-14 2003-08-26 Geron Corporation Promoter for telomerase reverse transcriptase
US6777203B1 (en) * 1997-11-19 2004-08-17 Geron Corporation Telomerase promoter driving expression of therapeutic gene sequences
ES2127697B1 (es) * 1997-03-05 2000-03-16 Antibioticos Sau Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.
US7378244B2 (en) * 1997-10-01 2008-05-27 Geron Corporation Telomerase promoters sequences for screening telomerase modulators
ES2143408B1 (es) * 1998-04-02 2000-12-01 Urquima Sa Promotor y construcciones para expresion de proteinas recombinantes en hongos filamentosos.
WO2000052176A1 (de) * 1999-03-03 2000-09-08 Cilian Ag β-HEXOSAMINIDASE SOWIE DIESE KODIERENDE DNA-SEQUENZ AUS CILIATEN UND DEREN VERWENDUNG
US20090104165A1 (en) * 2004-09-22 2009-04-23 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
EP1801221A1 (en) * 2005-12-22 2007-06-27 Sandoz AG Promoter sequences
CN102127546B (zh) * 2010-11-24 2012-10-24 山东农业大学 一种骨骼肌特异性actin启动子及其应用
CN116004892B (zh) * 2022-10-31 2025-04-29 中国热带农业科学院环境与植物保护研究所 一种香蕉灰纹病菌的pcr检测引物及检测方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8508800D0 (en) * 1985-04-04 1985-05-09 Glaxo Group Ltd Microbiological process
FI864473L (fi) * 1985-11-20 1987-05-21 Panlabs Inc Sammansatta yttryckskassetter foer fungaltransformering.
ES2127697B1 (es) * 1997-03-05 2000-03-16 Antibioticos Sau Promotores de los genes glutamato deshidrogenasa, beta-n-acetilhexosaminidasa y gamma-actina y su utilizacion en sistemas de expresion, secrecion y antisentido de hongos filamentosos.

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
JOHN ME: "Structural characterization of genes corresponding to cotton fiber mRNA, E6: reduced E6 protein in transgenic plants by antisense gene", PLANT MOL BIOL., 1996, pages 297 - 306
KINNAIRD JH, FINCHAM JR.: "The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) gene.", GENE, 1983, pages 253 - 260, XP023542610, DOI: doi:10.1016/0378-1119(83)90195-6
KOLAR M. ET AL.: "Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene", GENE, 1988, pages 127 - 134, XP023544459, DOI: doi:10.1016/0378-1119(88)90586-0
KUNKEL, W. ET AL.: "Genetic instability of industrial strains of Penicillium chrysogenum", APPL. MICROBIOL. BIOTECHNOL, 1992, pages 499 - 502

Also Published As

Publication number Publication date
US6300095B1 (en) 2001-10-09
ES2149706B1 (es) 2001-05-16
KR20000065202A (ko) 2000-11-06
LT98152A (en) 1999-06-25
ES2149707A1 (es) 2000-11-01
CZ353598A3 (cs) 1999-02-17
ES2149706A1 (es) 2000-11-01
US6558921B1 (en) 2003-05-06
EE9800381A (et) 1999-04-15
ZA981896B (en) 1998-09-14
CN1219200A (zh) 1999-06-09
PL329722A1 (en) 1999-04-12
LV12264A (lv) 1999-04-20
AU6101198A (en) 1998-09-22
HUP0000870A3 (en) 2001-09-28
IL126647A0 (en) 1999-08-17
WO1998039459A1 (es) 1998-09-11
ES2149707B1 (es) 2001-05-16
LV12264B (en) 1999-10-20
ES2127697A1 (es) 1999-04-16
SK149598A3 (en) 2000-03-13
ES2127697B1 (es) 2000-03-16
HUP0000870A2 (en) 2000-07-28
CA2253250A1 (en) 1998-09-11
JP2000509613A (ja) 2000-08-02

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